β-Carotene과 알코올을 섭취한 쥐의 혈액 성분변화의 상관관계

고재웅,조만희,이상한,우기민,염윤기,김창세 순천향의학연구소 2000 Journal of Soonchunhyang Medical Science Vol.6 No.2

The study was carried out to investigate the effects of dietary carotenids and β-carotene on the hematological changes caused by alcohol administration into the rats. The results were analyzed using several statistical methods; SPSS computer program for measuring the mean values and standard deviations, one way ANOVA for the test of significance, Duncan method for post HOC multiple comparison, and Pearson method for measuring the correlation coefficient. The following are the conclusion from those statistical analysis. 1. ALB and TB were shown to have the most significant correlation among the blood compositions f개m the basic dietary groups. 2. BUN and CHO were shown to have the most positive correlation among those from the carrot-fed groups. 3. CA and AG were shown positively correlated, and CHO and IP negatively correlated, respectively among those from the cheese-fed groups. 4. AP was shown positively correlated among those from the alcohol-administrated groups. 5. CHO, CA and BUN were shown positively correlated, and CA was negatively correlated among those from the β-Carotene -fed groups. 6. TB and TP were not affected by the correlations between the blood composition from all the experimental groups.

다중 제어루프에 의한 자기부상용 DC-DC 전원장치에 관한 연구

조주현,조정민,전기영,이상집,이승환,오봉환,이훈구,한경희 明知大學校 産業技術硏究所 2004 産業技術硏究所論文集 Vol.23 No.-

The author present a modified multi-loop algorithm including feedforward for controlling a 55kW step down chopper in the power supply of Maglev. The control law for the duty cycle consists of three terms. The first is the feedforward term which compensates for variations in the input voltage. The second term consists of the difference between the slowly moving inductor current and output current. The third term consists of proportional and integral terms involving the perturbation in the output voltage. This perturvation is derived by subtracting the desired output voltage from the actual output voltage. The proportional and integral action stabilizes the system and minimizes output voltage error. In order to verify the validity of the proposed multi-loop controller, simulation study was tried using Matlab simulink.

Al-Zn-Mg合金의 加工熱處理에 미치는 遷移元素의 影響

趙顯驥,李基大,金熙都 慶北大學校 1982 論文集 Vol.33 No.-

An investigation for influence of a small additions of transition metals (Mn, Zr, Cr) on the thermomechanical treatment (TMT) in Al-Zn-Mg alloys has been carried out by means of the measurements of tensile strength and micro-vickers hardness. The results obtained from this experiment are summarized as follows; 1) Hardness and strength of Al-Zn-Mg alloys are increased remarkable by a small additions of transition metals (Mn, Zr, Cr) at as-quenched state. 2) Influence of a small additions of transition metals like Mn, Zr and Cr added to Al-Zn-Mg alloys on ageing phenomena was retarding the ageing character. 3) The mechanical properties (hardness and strength) of Al-Zn-Mg alloys which were processed by TMT are increased when the deformation rate is lower than 30%, on the other hand those of Al-Zn-Mg alloys added transition metals were decreased with increasing deformation rate.

Carotenoids 식이와 알코올이 혈액학적 성분과 간조직에 미치는 영향에 관한 연구

조만희,김연선,이상한,우기민,장예진,김창세 순천향의학연구소 1999 Journal of Soonchunhyang Medical Science Vol.5 No.2

Alcohol is well known agent which can damage the human tissues such as liver via stimulating lipid peroxidation and storage, denaturation of macromolecules, and inhibiting protein metabolism. On the other hand, carotenoids in addition to vitamins A, C, E and lipoic acid, play important roles in protecting these oxidative damages as well as preventing the production of free radicals. This study was carried out to elucidate the precise effects of alcohol administration into rats on the antioxidative functions of dietary carotenoids and isolated β-carotene, and to find out any parameters to uncover more detailed biochemical mechanisms of these agents. For these purposes, two different approaches were performed: 1) examination of the changes in hematological parameters (e.g., total proteins, A/G ratio, ALT/AST et. al) and statistical correlations among inter- and intragroups, 2) examination of the histopathological changes by an electron microscope. The results were analyzed and summarized as following; (1) Carrot diet for one week caused a slight increase in albumin. A/G ratio and AST levels, and a slight decrease in ALT, ALP, BUN and uric acid levels. Two-week alcohol administration following carrot diet increased total proteins, albumin A/G ratio and BUN levels. However, both groups were shown to have little significant changes in cholesterol concentrations. (2) Cheese diet for one week caused a significant increase in total proteins, albumin, AST, ALP, uric acid and total cholesterol levels, and a significant decrease in A/G ratio and glucose concentrations. However, two-week β-carotene diet following cheese intake increased total proteins albumin, A/G ratio, BUN and total cholesterol levels, whereas AST, ALT, ALP and uric acid levels were decreased. (3) Alcohol administration for one week caused an increase in AST activities and a decrease in total proteins, albumin, ALT, ALP and BUN levels. A successive β-carotene diet following alcohol administration increased total proteins, albumin, ALT, ALP and BUN levels, whereas decreased AST and uric acid levels. However, there were not significant changes in A/G ratio, glucose and total cholesterol levels in both groups. (4) β-carotene diet for one week caused a slight increase in albumin, glucose, AST and uric acid levels, and a slight decrease in ALP and BUN levels. Two-weeks alcohol administration following β-carotene diet increased albumin, glucose, BUN and total cholesterol levels, whereas decreased AST, ALT, ALP and uric acid levels. (5) Although the pathological investigation on the liver did not reveal significant changes, cheese diet group (CH-BC/1-CH) was shown to have some lipid deposits. Some results were unexpected and different from typical hematological changes shown by other researchers. Nonetheless, these results strongly suggest that the liver damage or hepatism caused by alcohol intake affects many kinds of biochemical metabolisms, which results in significant changes in many hematological parameters. In addition, dietary carotenoid and isolated β-carotene were shown to have protective roles against the biochemical changes by alcohol intake.

알코올 및 베타 카로틴이 혈액 성분에 미치는 영향에 관한 연구

조만희,우기민,김창세,장예진,이상한,조성호 순천향의학연구소 1997 Journal of Soonchunhyang Medical Science Vol.3 No.2

Human take in many kinds of foods, and also are exposed to various chemicals which may be harmful. Especially, some food and chemicals may give rise to free radicals during metabolism in the tissues. These free radicals, such as superoxide. hydroxy radical and hydrogen peroxide, induce oxidations of the macromolecules, which can cause a variety of damages in the body. But human have anti-oxidative mechanisms well developed against these damages. Anti-oxidants such as vitamines A, C, E, and β-carotene are believed to have a major role in these mechanisms acting as a cofactor of anti-oxidative system. On the basis that alcohol is one of the most common chemicals that induce oxidative damages in liver, this study was carried out to elucidate biochemical changes of blood after alcohol administration as well as to investigate the effects of β-carotene on the alcohol-injected Sprague-Dawley rats. The following results were obtained from this study. At first, the number of WBC after one week were lowest in the alcohol-treated group. But, β-carotene treatment by oral injection was shown to diminish the increasing rate of WBC in the alcohol-treated group. Second, RBC was increased all the time in all groups tested with alcohol and/or β-carotene. It is of interest that there was a sudden increase of RBC in the alcohol-treated group. Third, Hb was increased in the alcohol-treated groups on the second week. On the other hand, the groups of β-carotene treatment showed more higher rate of increase in Ht and MCV. It is of note worthy that these effects were maximized in the group of β-carotene treatment followed by diet with cheese for a week. Finally, alcohol had little or no effect on MCH and MCHC, although β-carotene showed atypical effects. These results made it clear that alcohol can cause damages in liver, resulting in inflammation and increase of RBC. Also β-carotene is believed to protect liver from oxidative effects by alcohol. In according to these results, it is suggested that diet of anti-oxidants such as Vt. A, B, C, E, and β-carotene is strongly recommended to prevent certain diseases caused by oxidative damages.

원발성 위암조직에서 fragile histidine triad 유전자의 이상 발현에 대한 연구

조이현,이상한,우기민,송호연,조만희 순천향의학연구소 2001 Journal of Soonchunhyang Medical Science Vol.7 No.2

Background : The fragile histidine triad(FHIT) gene, located at human chromosome 3p14.2, has been shown to produce aberrant transcripts in a number of human cancers. Also, FHIT protein is supposed to have tumor suppressor function. Methods : To determine the role of the FHIT gene in gastric carcinoma, FHIT mRNA transcripts were examined in 16 primary adenocarcinomas by reverse transcription-polymerase chain reaction(RT-PCR). Expression of FHIT protein was measured by immunohistochemistry. Results : In this study, we found normal and abnormal RT-PCR products in 7 cases, and no transcript in one case. Positive immunostaining was observed in normal gastic gland, whereas FHIT immunoreactivity was significantly decreased or absent in most cases tested. Gastric cancer is thought to be develop from carcinogenic exposure, possibly accompanying with the high frequency of the FHIT gene abnormalities. Conclusion : The above results suggest that the FHIT gene can be a preferential target of environmental carcinogens, and the FHIT inactivation may play a role in the gastric carcinogenicity.

노인정신장애 평가척도(Psychogeriatric Assessment Scale)의 한국판 표준화 연구

조맹제,박임순,신영민,김무진,정희연,정은기,최용성,조성진,서국희,함봉진 大韓神經精神醫學會 2000 신경정신의학 Vol.39 No.1

연구목적 : 본 연구는 치매와 우울증을 다차원적으로 평가할 수 있는 도구인 Psychogeriatric Assessment Scale(PAS)의 한국판(PAS-K)을 개발하기 위한 것이다. 방 법 : 번역, 역번역, 그리고 세차례의 예비조사를 통하여 PAS-K를 제작하였다. 60세 이상의 노인 291명(임상환자군 : 58명, 지역사회노인군 : 168명, 수용시설 거주자 : 67명)과 그들의 정보제공자들을 대상으로 PAS-K, Geriatric Depression Scale(GDS), Korean version of Mini-Mental State Examination(MMSE-K), Hamilton Rating Scale for Depression(HRSD), 그리고 Hachinski Ischemic Score 를 적용하고, Diagnostic Interview chedule(DIS-Ⅲ-R)을 사용하여 치매와 우울증을 진단하였다. PAS-K의 신뢰도와 타당도를 평가하고, 예민도와 특이도, Kappa값, 그리고 ROC커브 분석법을 이용하여 PAS-K의 6개의 소척도들(피검자면접 : 인지기능 장애척도, 우울척도, 뇌졸중척도, 정보제공자면접 : 인지기능 저하척도, 행동변화척도, 뇌졸중척도)의 최적 절단점을 구하였다. 결 과 : PAS-K는 높은 내적 일관성을 보였고, 검사자간 신뢰도도 만족할 만한 수준이었다. PAS-K의 소척도들을 다른 표준화된 검사도구들과 비교하였을 때 인지기능 장애척도와 인지기능 저하척도는 MMSE-K와, 우울척도는 GDS 및 HRSD와 유의한 상관관계를 보였으며, 뇌졸중척도는 Hachinski Ischemic Score와 통계적으로 의미있는 상관관계를 보였다. 최적절단점은 인지기능 장애척도 10점, 인지기능 저하척도 3점, 우울척도 5점, 피검자면접과 정보제공자 뇌졸중척도 모두 1점, 그리고 행동변화척도 2점으로 추정하였다. 결 론 : 본 연구를 통해 PAS-K의 신뢰도와 타당도가 검증되었고, 노인정신의학 역학연구나 보건분야에서 치매와 우울증의 임상경과관찰 및 일차 선별도구로서 유용하게 사용될 수 있게 되었다. Objectives : This study was designed to develop the Korean version of the Psychogeriatric Assessment Scale(PAS-K), a multidimensional screening tool for the dementia and depression. Methods : Through three times preliminary trials, the authors translated PAS into Korean. The PAS-K, Geriatric Depression Scale, Hamilton Depression Rating Scale, Hachinski Ischemic Scale and Mini-Mental State Examination-Korean version were administered to 291 subjects over the age of 60 and the same number of their informants(67 subjects were institutionalized, 168 subjects were in community, 58 subjects were psychiatric hospital patients). And the Diagnostic Interview Schedule(DSM-Ⅲ-R) was independently administered to exactly diagnose dementia and depression. The reliability and validity test, optimal cut-off point estimation for six each scale and ROC curve analyses were done to investigate the diagnostic validity of PAS-K. Results : Internal consistency and interrater reliability of the PAS-K were high. Concurrent validity of each scales of PAS-K was good in being measured with other standardized scales. The optimal cut-off points of each scale of the PAS-K were estimated as follows : 1) Stroke Scale of subject interview : 1, 2) Cognitive Impairment Scale of subject interview: 10, 3) Depression Scale of subject interview : 5, 4) Stroke Scale of informant interview : 1, 5) Cognitive Decline Scale of informant interview : 3, and 6) Behavioral Change Scale of informant : 2. conclusions : The PAS-K was valid and reliable screening tools for detecting dementia and depression. Therefore the PAS-K could be widely and extensively used in psychogeriatric epidemiological research or clinical setting of primary screening for dementia and depression.

면역학적 특성에 따른 yeast cytochrome c의 구조에 관한 연구

조경환,우기민,조만희,이상한,박현경,김창세 순천향의학연구소 2000 Journal of Soonchunhyang Medical Science Vol.6 No.2

The antiserum against yeast cytochrome c and horse heart cytochrome c was reacted immunologically with those from various species. The results were as followings; 1. The antiserum against horse heart cytochrome c was reacted immunologically with heart cytochrome c of horse, cow, rabbit, mouse, chicken, pigeon, frog, tuna, and guanaco, and were not with yeast and neurospora cytochrome c. 2. However, antiserum against yeast cytochrome c was only reacted immunologically with yeast cytochrome c, and was not mammals and vertebrates horse, cow, rabbit, mouse, chicken, pigeon, frog, tuna, guanaco, even neurospora 3. CNBr-digested peptides of horse heart cytochrome c were obtained 5 peaks, Ⅰ(1-80), Ⅱ(1-65), Ⅲ(66-104), Ⅳ(81-104), Ⅴ(66-80), and other peptide (1-50). Among them peptides Ⅰ and Ⅱ were weakly reacted immunologically with antiserum against horse heart cytochrome c. CNBr-digested peptides of horse heart cytochrome c were not reacted with antiserum against yeast cytochrome c. 4. From the above, the immunologically positive cross reaction was thought to be related with Ω loop D.

단백질 및 1가 양이온을 통한 proteasome의 활성조절에 관한 연구

우기민,이상한,조만희 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.1

Proteasome은 일반적으로 세포내 환경과 매우 상이한 조건하에서 연구된다 (실험용 완충액에는 1기가 이온들이나 여러 단백질들이 결여되어 있다). 특이하게도 본 연구결과 낮은 농도의 Na^(+)와 K^(+)는 20S proteasome의 단백질 분해 활성을 현저히 저하시키지만 26S proteasome의 단백질 분해 활성에는 크게 영향을 미치지 못하였다. Na^(+)와 K^(+)는 20S proteasome의 chymotrypsin 유사 분해활성을 1-2 mM의 Ki 값으로 매우 민감하게 억제하였다. 이에 비해 기타 다른 1가 양이온 (Li^(+), Rb^(+), 또는 Cs^(+))은 미미한 억제효과를 나타내고, 2가 양이온 (Mg^(2+))은 20S proteasome의 chymotrypsin 유사 분해활성을 오히려 활성화시켰다. 이러한 이온들은 trypsin 유사 활성과 post-acidic 활성에도 유사한 억제효과를 보였다. 또한, Na^(+)와 K^(+)는 20S proteasome에 의한 casein, oxidized RNase A, a-lactalbumin 같은 단백질들의 분해를 강력하게 억제시키지만, 26S proteasome에 의한 ATP-의존적 단백질 분해 활성에는 영향을 미치지 못한다. 일반적으로 세포내 단백질농도는 매우 높은 것으로 알려져 있는 바, proteasome의 분해활성에 미치는 단백질의 영향을 조사한 결과, 무작위의 다양한 단백질들(0.1㎎/㎖)도 1가 양이온(Na^(+)와 K^(+))의 경우와 같이 20S proteasome의 펩타이드 분해활성을 억제하였지만 26S proteasome의 활성에는 아무런 영향을 미치지 않았다. 이러한 억제작용은 경쟁적이지 않다. BSA나 ovalbumin 같은 비가수분해 단백질들이 20S proteasome의 펩타이드 분해활성을 강력히 억제한였으므로 이러한 억제현상이 비경쟁적이라는 것을 알 수 있다. 비록 Na^(+), K^(+)와 단백질들이 20S proteasome을 비활성화 형태로 유지시키지만, 0.02% SDS에 의한 20S proteasome의 활성증가를 막거나 억제시키지는 못하였다. 이 결과로 고농도의 이온과 단백질이 존재하는 세포내 조건에서는, 독립적인 20S proteasome은 잠재적으로 비활성화된 형태로 존재하며, 19S ATPase 복합체나 11S 같은 proteasome 활성복합체가 결합한 후에 비로소 26S proteasome의 활성화된 형태로 전환되는 기작을 갖는 것으로 여겨진다. 또한, Na^(+)나 K^(+), BSA나 ovalbuim의 첨가는 비활성화 형태의 20S proteasome을 이용한 많은 연구를 보다 용이하게 할 것으로 기대된다.

Lumbricus rubellus에 존재하는 키틴분해효소에 관한 연구

우기민,이상한,조만희 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.1

본 연구를 통하여 Lumbricus rubellus 지렁이가 chitinase, N-acetylglucos aminidase, 그리고 cellulase 등의 탄수화물 분해효소들을 지니고 있는 것으로 밝혀졌다. 30~80% Ammonium sulfate 분획법, 이온교환수지법, 소수성수지법, 젤여과수집법들을 이용하여 chitinase와 N-acetylglucosaminidase들을 부분적으로 정제하였다. 4-Methyl umberriferone이 결합된 형광성 기질을 이용하여 키틴 분해효소의 활성을 측정한 결과 triacetyl-chitotrioside, diacetyl-chitobioside와 N-acetylglucosamine등의 대표적인 기질들을 매우 높은 활성도로 분해하였다. 또한 키틴 분해효소들은 60℃의 높은 온도에서도 한 시간이상 변성되지 않고 기질분해 능력을 잃지 않았다. 더 놀라운 사실은 많은 효소들을 변성시키고 활성을 감소시키는 여러 가지 억제제들(SDS, sodium deoxycholate, Triton X-100, NaCl과 DTT)이 효소의 활성에 거의 영향을 미치지 않는다는 것이다. 특히 대부분의 효소를 완전히 변성시키는 4% 농도의 SDS도 chitinase의 활성을 겨우 30% 정도만 억제하였다. Lumbricus rubellus chitinase의 이러한 특이하고 독특한 생화학적 성질들은 Lumbricus가 산업적으로 매우 유용한 대사효소들(chitinases, lipases와 proteases)을 지니고 있으며, 의학을 비롯한 환경정화, 생물공정, 건강보조식품, 식품, 세제 등의 다양한 산업에 적용 가능하게 할 것으로 기대된다.