EFFECT OF THE STRUCTURE OF ISOMERS OF MERCAPTOVALERIC ACID ON OPTICAL PROPERTIES OF CdTe QUANTUM DOTS

KAI-GUO MA,JIN-YI BAI,TAN FANG,Haiqing Guo 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2013 NANO Vol.8 No.5

4-Mercaptovaleric acid (4MVA) and 3-mercaptovaleric acid (3MVA), two branched isomers of 5-mercaptovaleric acid (MVA), were designed and synthesized. They were used as capping agents in the aqueous synthesis of CdTe quantum dots (QDs) by a modified hydrothermal method with sodium tellurite as Te source. Compared with MVA-CdTe, either 4MVA-QDs or 3MVA-QDs exhibited higher fluorescent quantum yield (QY) and sharper absorption peak, which confirmed the superiority of using branched mercapto acids in the aqueous synthesis of QDs for improved optical properties. The fluorescence of QDs capped with 4MVA was higher than that of QDs capped with 4-mercaptobutyric acid (MBA) but lower than that of QDs capped with 3-mercaptobutyric acid (3MBA), which further confirmed the advantage of designing methyl side group rather than methylene group in the main chain for desired optical properties. However, the lower fluorescence, less sharp absorption peak and faster growth rate of 3MVA-QDs than that of 3MBA-QDs indicated adverse effect of side group on the aqueous synthesis of QDs if the size of side group is very bulky. Our results are helpful for selecting and designing appropriate capping agents for QDs with excellent properties.

An Automatic Urban Function District Division Method Based on Big Data Analysis of POI

( Hao Guo ),( Haiqing Liu ),( Shengli Wang ),( Yu Zhang ) 한국정보처리학회 2021 Journal of information processing systems Vol.17 No.3

Along with the rapid development of the economy, the urban scale has extended rapidly, leading to the formation of different types of urban function districts (UFDs), such as central business, residential and industrial districts. Recognizing the spatial distributions of these districts is of great significance to manage the evolving role of urban planning and further help in developing reliable urban planning programs. In this paper, we propose an automatic UFD division method based on big data analysis of point of interest (POI) data. Considering that the distribution of POI data is unbalanced in a geographic space, a dichotomy-based data retrieval method was used to improve the efficiency of the data crawling process. Further, a POI spatial feature analysis method based on the mean shift algorithm is proposed, where data points with similar attributive characteristics are clustered to form the function districts. The proposed method was thoroughly tested in an actual urban case scenario and the results show its superior performance. Further, the suitability of fit to practical situations reaches 88.4%, demonstrating a reasonable UFD division result.

Relationship between Sport Sponsorship Motivation, Attitude and Brand Loyalty among Chinese University Students

( Ce Guo ),( Wanghui Li ),( Qingyang Shao ),( Haiqing Yang ),( Zixiang Zhou ) 한국융합과학회 2023 한국융합과학회 국제학술대회자료집 Vol.2023 No.0

Root coverage using a coronally advanced flap with or without acellular dermal matrix: a meta-analysis

Wei Guan,Haiqing Liao,Li Guo,Changning Wang,Zhengguo Cao 대한치주과학회 2016 Journal of Periodontal & Implant Science Vol.46 No.1

Purpose: Gingival recession is a major esthetic concern and may lead to root sensitivity during periodontal treatment. Coronally advanced flaps (CAFs) with and without acellular dermal matrix (ADM) are widely used in root coverage procedures. The aim of this study was to analyze the efficacy of CAF in combination with ADM in the treatment of gingival recession. Methods: PubMed, The Cochrane Library, and Embase were used to identify relevant articles. The articles were screened, data were extracted, and the quality of the studies was assessed by three reviewers with expertise in clinical practice, trials, statistics, and biomedical editing. The clinical endpoints of interest included changes in recession, probing depth (PD), clinical attachment level (CAL), and keratinized tissue (KT). Results: Ten randomized controlled trials were identified, including six studies that compared CAFs with ADM and CAFs using connective tissue grafting (CTG) and four studies that compared CAFs with or without ADM. No statistically significant differences were found between the use of ADM and CTG, whereas statistically significant differences were found between groups in which ADM and CAF were combined and groups that underwent CAF alone with regard to recession coverage, CAL, and KT. The combination of CAF with an ADM allograft achieved more favorable recession coverage and recovery of CAL and KT than CAF alone. Conclusions: The results from the ADM and CTG groups suggest that both procedures may be equally effective in clinical practice. Given the limitations of this study, further investigation is needed to clarify the effectiveness of ADM and CAF in clinical practice.

덱스트란과 젤라틴을 고정화한 생의학용 산화철 나노입자의 제조

이상민,강인규,김선정,Haiqing Guo 한국생체재료학회 2008 생체재료학회지 Vol.12 No.1

Superparamagnetic iron oxide nanoparticles with suitable biocompatible molecules have been widely used experimentally for numerous in vivo applications such as magnetic resonance imaging contrast enhancement, tissue engineering, immunoassay, detoxification of biological fluids, hyperthermia, drug delivery, and in cell separation etc. Surface-functionalized magnetite nanoparticles are suitable for receptor targeting because of their colloidal stability, biocompatibility, biodegradability, and non-toxicity for the body. We have prepared gelatin-immobilized magnetite nanoparticles in order to examine their possible application to molecular imaging. The presence of gelatin on the surface of nanoparticles was confirmed by observing amine groups using FTIR and ESCA. The size of DGNP particles measured by TEM and DLS was about 7.5 and 25 nm, respectively. In the cell culture experiment, it is revealed that DGNPs are more effectively uptaken by fibroblasts when compared to DNP.

Root coverage using a coronally advanced flap with or without acellular dermal matrix: a meta-analysis

Guan, Wei,Liao, Haiqing,Guo, Li,Wang, Changning,Cao, Zhengguo Korean Academy of Periodontology 2016 Journal of Periodontal & Implant Science Vol.46 No.1

Purpose: Gingival recession is a major esthetic concern and may lead to root sensitivity during periodontal treatment. Coronally advanced flaps (CAFs) with and without acellular dermal matrix (ADM) are widely used in root coverage procedures. The aim of this study was to analyze the efficacy of CAF in combination with ADM in the treatment of gingival recession. Methods: PubMed, The Cochrane Library, and Embase were used to identify relevant articles. The articles were screened, data were extracted, and the quality of the studies was assessed by three reviewers with expertise in clinical practice, trials, statistics, and biomedical editing. The clinical endpoints of interest included changes in recession, probing depth (PD), clinical attachment level (CAL), and keratinized tissue (KT). Results: Ten randomized controlled trials were identified, including six studies that compared CAFs with ADM and CAFs using connective tissue grafting (CTG) and four studies that compared CAFs with or without ADM. No statistically significant differences were found between the use of ADM and CTG, whereas statistically significant differences were found between groups in which ADM and CAF were combined and groups that underwent CAF alone with regard to recession coverage, CAL, and KT. The combination of CAF with an ADM allograft achieved more favorable recession coverage and recovery of CAL and KT than CAF alone. Conclusions: The results from the ADM and CTG groups suggest that both procedures may be equally effective in clinical practice. Given the limitations of this study, further investigation is needed to clarify the effectiveness of ADM and CAF in clinical practice.

Anti-proliferation and pro-apoptosis effects of miR-582-5p in chronic lymphocytic leukemia via targeting HNRNPA1 and suppression of NF-κB

Wang Zengsheng,Li Yan,Kuang Xiaochuan,Guo Fang,Lang Tao,Mao Min,Zhang Xiaoyan,Yang Haiqing 대한독성 유전단백체 학회 2021 Molecular & cellular toxicology Vol.17 No.3

Background MicroRNAs (miRNAs) function as post-transcriptional mediators for genes involved in cancer progression, including chronic lymphocytic leukemia. MiR-582-5p has been identified as a tumor suppressor in various tumors. The antioncogenic role of miR-582-5p was then validated in this study. Objective Mononuclear cells were isolated from peripheral blood samples of patients with chronic lymphocytic leukemia and healthy donors. Expression of miR-582-3p in the mononuclear cells was examined by qRT-PCR. CCK8 assay was performed to detect cell viability, and cell cycle and apoptosis were evaluated by flow cytometry. Dual luciferase activity assay was performed to determine the targeting relationship between miR-582-3p and HNRNPA1, and western blot was performed to unravel the mechanism. Results MiR-582-5p was reduced in mononuclear cells of patients with chronic lymphocytic leukemia compared to healthy donors. Forced miR-582-5p expression reduced cell viability, and promoted apoptosis of chronic lymphocytic leukemia cells. Cell cycle was arrested in G0/G1 phase via miR-582-5p mimic. MiR-582-5p bound to HNRNPA1 (heterogeneous nuclear ribonucleoprotein A1) and down-regulated its expression. Silence of HNRNPA1 decreased cell viability, promoted apoptosis, and blocked cell cycle at G0/G1 phase through up-regulation of IκBα (IkappaBalpha). Moreover, HNRNPA1 silencing attenuated the promotive effect induced by miR-582-5p inhibitor on the progression of chronic lymphocytic leukemia. Conclusion MiR-582-5p demonstrated anti-proliferative and pro-apoptotic roles in chronic lymphocytic leukemia cell growth via down-regulation of HNRNPA1 and up-regulation of IκBα, thus inactivating NF-κB. Background MicroRNAs (miRNAs) function as post-transcriptional mediators for genes involved in cancer progression, including chronic lymphocytic leukemia. MiR-582-5p has been identified as a tumor suppressor in various tumors. The antioncogenic role of miR-582-5p was then validated in this study. Objective Mononuclear cells were isolated from peripheral blood samples of patients with chronic lymphocytic leukemia and healthy donors. Expression of miR-582-3p in the mononuclear cells was examined by qRT-PCR. CCK8 assay was performed to detect cell viability, and cell cycle and apoptosis were evaluated by flow cytometry. Dual luciferase activity assay was performed to determine the targeting relationship between miR-582-3p and HNRNPA1, and western blot was performed to unravel the mechanism. Results MiR-582-5p was reduced in mononuclear cells of patients with chronic lymphocytic leukemia compared to healthy donors. Forced miR-582-5p expression reduced cell viability, and promoted apoptosis of chronic lymphocytic leukemia cells. Cell cycle was arrested in G0/G1 phase via miR-582-5p mimic. MiR-582-5p bound to HNRNPA1 (heterogeneous nuclear ribonucleoprotein A1) and down-regulated its expression. Silence of HNRNPA1 decreased cell viability, promoted apoptosis, and blocked cell cycle at G0/G1 phase through up-regulation of IκBα (IkappaBalpha). Moreover, HNRNPA1 silencing attenuated the promotive effect induced by miR-582-5p inhibitor on the progression of chronic lymphocytic leukemia. Conclusion MiR-582-5p demonstrated anti-proliferative and pro-apoptotic roles in chronic lymphocytic leukemia cell growth via down-regulation of HNRNPA1 and up-regulation of IκBα, thus inactivating NF-κB.

Albumin-conjugated Cadmium Sulfide Nanoparticles and their Interaction with KB Cells

Selim, K.M. Kamruzzaman,Kang, Inn-Kyu,Guo, Haiqing The Polymer Society of Korea 2009 Macromolecular Research Vol.17 No.6

Cytotoxicity is a severe problem of cadmium sulfide nanoparticles(CSNPs) for use in biological systems. In the present study, mercaptoacetic acid-coated CSNPs were conjugated with bovine serum albumin (BSA) to improve biocompatibility. The surface properties of the CSNPs and albumin-conjugated CSNPs (ACSNPs) were characterized by XRD, UV, FTIR, EA, TEM and DLS. Human breast cancer cells (KB cells) were then cultured in the presence of the nanoparticles to evaluate the cytotoxicity of CSNPs and ACSNPs. Finally, the fluorescence intensity of the nanoparticles' aqueous solution was examined using a fluorescence spectrometer. The results showed that the cell compatibility and fluorescence intensity of ACSNPs were higher than those of CSNPs. The strongly luminescent features of the biocompatible ACSNPs are promising for use in biological fields such as cellular labeling, intracellular tracking and molecular imaging.

Immobilization of lactobionic acid on the surface of cadmium sulfide nanoparticles and their interaction with hepatocytes.

Kamruzzaman Selim, K M,Xing, Zhi-Cai,Guo, Haiqing,Kang, Inn-Kyu Chapman and Hall ; Kluwer Academic Publishers 2009 Journal of materials science, Materials in medicin Vol.20 No.9

<P>In the current study, beta-galactose-carrying lactobionic acid (LA) was conjugated on the surface of mercaptoacetic acid-coated cadmium sulfide nanoparticles (CSNPs) to ensure specific recognition of liver cells (hepatocytes) and to enhance biocompatibility. Maltotrionic acid-coated CSNPs (MCSNPs) were also prepared for use as a control. The results showed that LA-immobilized CSNPs (LCSNPs) were selectively and rapidly internalized into hepatocytes and emitted more intense fluorescence images as well as demonstrated increased biocompatible behavior in vitro than those of CSNPs and MCSNPs. Furthermore, the uptake amount of LCSNPs into hepatocytes was higher than that of CSNPs and MCSNPs. All these results indicate that LCSNPs may find ever-growing applications in biological labels and detection or contrast agents in life science and medical diagnostics.</P>

Surface Modification of Magnetites Using Maltotrionic Acid and Folic Acid for Molecular Imaging

Selim, K.M.Kamruzzaman,Lee, Joo-Hee,Kim, Sun-Jung,Xing, Zhicai,Kang, Inn-Kyu,Chang, Yong-Min,Guo, Haiqing The Polymer Society of Korea 2006 Macromolecular Research Vol.14 No.6

Highly hydrophilic, uniform, superparamagnetic and nontoxic maltotrionic acid (MA)-coated magnetite nano-particles (MAM) were prepared and characterized by TEM, DLS, XRD and VSM. MA was used to improve the biocompatibility, monodispersity and non-specific intracellular uptake of nanoparticles. Folic acid (FA) was subsequently conjugated to the MAM to preferentially target KB cells (cancer cells) that have folate receptors expressed on their surfaces and to facilitate nanoparticles in their transit across the cell membrane. Finally, fluorescence isothiocyanate (FITC) was added to the nanoparticles to visualize the nanoparticle internalization into KB cells. After the cells were cultured in a media containing the MAM and MAM-folate conjugate (FAMAM), the results of fluorescence and confocal microscopy showed that both types of nanoparticles were internalized into the cells. Nevertheless, the amount of FAMAM uptake was higher than that of MAM. This result indicated that nanoparticles modified with MA and FA could be used to facilitate the nanoparticle uptake to specific KB cells (cancer cells) for molecular imaging.