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Anticancer effects of D-pinitol in human oral squamous carcinoma cells
Shin, Hyun-Chul,Bang, Tea-Hyun,Kang, Hae-Mi,Park, Bong-Soo,Kim, In-Ryoung The Korean Academy of Oral Biology 2020 International Journal of Oral Biology Vol.45 No.4
D-pinitol is an analog of 3-methoxy-D-chiro-inositol found in beans and plants. D-pinitol has anti-inflammatory, antidiabetic, and anticancer effects. Additionally, D-pinitol induces apoptosis and inhibits metastasis in breast and prostate cancers. However, to date, no study has investigated the anticancer effects of D-pinitol in oral cancer. Therefore, in this study, whether the anticancer effects of D-pinitol induce apoptosis, inhibit the epithelial-to-mesenchymal transition (EMT), and arrest cell cycle was investigated in squamous epithelial cells. D-pinitol decreased the survival and cell proliferation rates of CAL-27 and Ca9-22 oral squamous carcinoma cells in a concentration- and time-dependent manner. Evidence of apoptosis, including nuclear condensation, poly (ADP-ribose) polymerase, and caspase-3 fragmentation, was also observed. D-pinitol inhibited the migration and invasion of both cell lines. In terms of EMT-related proteins, E-cadherin was increased, whereas N-cadherin, Snail, and Slug were decreased. D-pinitol also decreased the expression of cyclin D1, a protein involved in the cell cycle, but increased the expression of p21, a cyclin-dependent kinase inhibitor. Hence, D-pinitol induces apoptosis and cell cycle arrest in CAL-27 and Ca9-22 cells, demonstrating an anticancer effect by decreasing the EMT.
Anorectal Malformations Associated with Esophageal Atresia in Neonates
Byun, Shin Yun,Lim, Ryoung Kyoung,Park, Kyung Hee,Cho, Yong Hoon,Kim, Hae Young The Korean Society of Pediatric Gastroenterology 2013 Pediatric gastroenterology, hepatology & nutrition Vol.16 No.1
Purpose: Anorectal malformations are often associated with other anomalies, reporting frequency with 40-70%. Gastrointestinal anomalies have been known to be relatively less common than associated anomalies of other organ system. This study was performed to assess a distinctive feature of cases associated with esophageal atresia. Methods: Clinical data (from January 2000 through December 2011) on the 196 subjects with anorectal malformations, managed in our Hospital, were reviewed. Total 14 neonates were identified with accompanying esophageal atresia and retrospective analysis was conducted. Results: The incidence was 7.1% and there were 8 male and 6 female subjects. Only 2 cases were associated with esophageal atresia without tracheoesophageal fistula. Although variable cases of anorectal malformation in female subjects, almost cases were anorectal malformations with rectourethral fistula in male. Other associated anomalies were identified in all cases, with more than 3 anomalies in 10 cases. There were 4 VACTERL (Vertebral abnormalities, Anal atresia, Cardiac anomalies, Tracheoesophageal fistula, Esophageal atresia, Renal and Limb anomalies) associations accounting for 28.6%, but could not identify chromosomal anomaly. Most cases were managed with staged procedure, usually primary repair of esophageal atresia and diverting colostomy. Overall mortality rate was 21.4%, mainly caused by heart problems. Conclusion: This study shows that early diagnosis and rational surgical approach with multidisciplinary plan are mandatory in managing anorectal malformations with esophageal atresia, when considering a high frequency of associated anomaly and a relative high mortality.
The Effects of Kaempferol-Inhibited Autophagy on Osteoclast Formation
Kim, Chang-Ju,Shin, Sang-Hun,Kim, Bok-Joo,Kim, Chul-Hoon,Kim, Jung-Han,Kang, Hae-Mi,Park, Bong-Soo,Kim, In-Ryoung MDPI 2018 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.19 No.1
<P>Kaempferol, a flavonoid compound, is derived from the rhizome of <I>Kaempferia galanga L</I>., which is used in traditional medicine in Asia. Autophagy has pleiotropic functions that are involved in cell growth, survival, nutrient supply under starvation, defense against pathogens, and antigen presentation. There are many studies dealing with the inhibitory effects of natural flavonoids in bone resorption. However, no studies have explained the relationship between the autophagic and inhibitory processes of osteoclastogenesis by natural flavonoids. The present study was undertaken to investigate the inhibitory effects of osteoclastogenesis through the autophagy inhibition process stimulated by kaempferol in murin macrophage (RAW 264.7) cells. The cytotoxic effect of Kaempferol was investigated by MTT assay. The osteoclast differentiation and autophagic process were confirmed via tartrate-resistant acid phosphatase (TRAP) staining, pit formation assay, western blot, and real-time PCR. Kaempferol controlled the expression of autophagy-related factors and in particular, it strongly inhibited the expression of p62/SQSTM1. In the western blot and real time-PCR analysis, when autophagy was suppressed with the application of 3-Methyladenine (3-MA) only, osteoclast and apoptosis related factors were not significantly affected. However, we found that after cells were treated with kaempferol, these factors inhibited autophagy and activated apoptosis. Therefore, we presume that kaempferol-inhibited autophagy activated apoptosis by degradation of p62/SQSTM1. Further study of the <I>p62/SQSTM1</I> gene as a target in the autophagy mechanism, may help to delineate the potential role of kaempferol in the treatment of bone metabolism disorders.</P>
Effect of Salinity, Temperature, and Glucose on the Production of Vibrio vulnificus Hemolysin
Kim, Hyun-Soo,Shin, Sung-Heui,Park, Hae-Ryoung,Lee, Shee-Eun,Kim, Choon-Mee,Kim, Soo-Young,Kim, Young-Ran,Lee, Hyun-Chul,Chung, Sun-Sik,Rhee, Joon-Haeng The Korean Society for Microbiology 2002 Journal of Bacteriology and Virology Vol.32 No.4
Among the exotoxins produced by V. vulnificus, hemolysin (HS) has been reported to be the most potent one. To investigate the factors up- or down-regulating HS production in the context of pathogenesis, we observed the effects of salinity or/and temperature shifting, glucose, and acidic pH on the production of HS by V. vulnificus C7184 strain in vitro. Significantly more HS was produced when V. vulnificus was cultured in 0.9% salinity and $37^{\circ}C$ than in 2.5% and $25^{\circ}C$. When the culture condition reflecting natural habitat of V. vulnificus (2.5% salinity and $25^{\circ}C$) was changed into that reflecting human body (0.9% salinity and $37^{\circ}C$), 2.5 fold or more HS was produced than in the V. vulnificus being cultured continuously in 0.9% NaCl at $37^{\circ}C$. This result suggests that V. vulnificus somehow recognizes the shifting in salinity and temperature and stimulate HS production. Glucose addition in the culture medium resulted in a dose-dependent decrease in the HS production. Glucose itself and acidic pH resulting from its metabolism both appeared to inhibit the HS production. Glucose in itself had more dominant role in suppressing the HS production than the lowered pH accompanying the metabolism of glucose. This result suggests that HS production is down-regulated in the presence of glucose and under environmental acidic pH.
Joung, Hosouk,Kwon, Jin-Sook,Kim, Ju-Ryoung,Shin, Sera,Kang, Wanseok,Ahn, Youngkeun,Kook, Hyun,Kee, Hae Jin Elsevier 2012 Atherosclerosis Vol.222 No.1
<P><B>Highlights</B></P><P>► Enhancer of polycomb1 (Epc1) attenuates VSMC proliferation. ► Epc1 induces VSMC differentiation. ► Epc1 interact with myocardin to activate smooth muscle 22α promoter activity. ► Epc1 gene delivery reduces neointima formation induced by balloon injury.</P> <P><B>Abstract</B></P><P><B>Objective</B></P><P>Previously, we reported that enhancer of polycomb1 (Epc1) induces skeletal muscle differentiation through the serum response factor (SRF). Considering that SRF plays a critical role in vascular smooth muscle cell (VSMC) differentiation, we expected that Epc1 also works in VSMCs. Here we examined the effect of Epc1 on neointima formation after arterial balloon injury and the underlying mechanism.</P><P><B>Methods</B></P><P>Epc1 expression was examined in carotid artery injury or VSMC models. Interaction with myocardin (Myocd), a master regulator of smooth muscle differentiation, was examined by immunoprecipitation or promoter analysis with smooth muscle (SM) 22α promoter. Finally, we investigated whether local delivery of Epc1 regulated neointimal formation after injury.</P><P><B>Results</B></P><P>Epc1 expression was down-regulated during proliferation induced by platelet-derived growth factor BB, whereas it was upregulated during differentiation in VSMCs. Forced expression of Epc1 induced VSMC differentiation. Epc1 physically interacted with Myocd to synergistically activate SM22α promoter activity. Transient transfection of Epc1 enhanced the physical interaction between Myocd and SRF, whereas that interaction was reduced when A10 cells were treated with siRNA for Epc1. Local delivery of Epc1 significantly reduced neointima formation induced by balloon injury.</P><P><B>Conclusions</B></P><P>Our results indicate that Epc1 induces VSMC differentiation by interacting with Myocd to induce SRF-dependent smooth muscle genes. We propose that Epc1 acts as a novel negative regulator of neointima formation after carotid injury.</P>
5, 8-Quinolinedione derivatives의 항진균 작용과 합성
권영,김진영,신성희,이해령,김희정 이화여자대학교약학회 1994 梨花藥學硏究 Vol.- No.33
In order to evaluate the antifungal, antibacterial, anticancer effect of 5, 8-quinolinedione derivatives, we newly synthesized several 6-(N-arylamino)-7-chloro-5, 8-quinolinedione derivatives. 6, 7-Dichloro-5, 8-quinolinediones were prepared by treating 5-amino-8-hydroxyquinoline with KClO_3 in HCl, substitution of 6, 7-dichloro-5, 8-quinolinedione with aryl amines in ethanol gave a 6-(N-arylamino)-7chlore-5, 8-quinolinedionse with 80~90% yield. These derivatives were subjected to antibacterial and antifungal activity test, in vitro, against Candida albicans ATCC 10231, Candida albicans Local, Aspergillus niger KCTC 1231, Tricophyton mentagrophytes KCTC 6085. The MIC value was determined by the two-fold agar/streak dilution method. 6-(N-arylamino)-7-chloro-5, 8-quiolinedione derivatives showed good antifungal activities in vitro. These derivatives, R1-4 showed more potent antifungal activities than fluconazole, ketoconazole and griseofulvin.