四君子湯 抽出液이 家兎의 體液性 免疫反應에 미치는 影響

林圭庠,文炳淳,李起男 圓光大學校 醫科學硏究所 1987 圓光醫科學 Vol.3 No.1

The present experiment was investigated on the effects of Sagunjatang extract on the humoral immune response of rabbit. In order to understand the immune respone of the extract, one of the experimental groups was treated with the antigen, E. coli Ry 13 suspension and the extract. And the serum separated from the treated group was analyzed. The results obtained were as follows : 1. The body weight of the rabbit treated with Sagunjatang extract was increased remarkably. 2. Sagunjatang extract did not affect the number of erythrocytes, however, it increased the number of WBC in small amount. It means that the extract activates the WBC related with producing antibody. 3. The synthesis of γ-globulin was stimulated by the Sagunjatang extract in the present experiment.

四君子湯 抽出液이 筋小胞體의 ATPase 活性에 미치는 影響에 關한 硏究

李起男,林圭庠 圓光大學校 醫科學硏究所 1985 圓光醫科學 Vol.1 No.2

The present experiment was investigated on the effects of SaGunJaTang extract(SGJex) on the sarcoplasmic reticulum ATPase activity. The result demonstrated that the ATPase activity of sarcoplasmic reticulum was activated in the presence of SaGunJa extract, the activation effect of the extract was analyzed with time course, Lineweaver -Burk plot, and Arrhenius plot. The activation mechanism of SGJex on the enzyme was proposed by increasing the affinity between active site of the enzyme and ATP prolecules, and the energy of activation was decreased in the presence of SaGunJa extract. The results were obtained as follows : 1. The sarcoplasmic reticulum ATPase was free from the mitochondrial contamination in the present experiment, the optimum pH was 6.8 and the optimum concentrations of Ca^++, Mg^++ and SaGunJa extract were 0.1mM 4mM and 1% respectively. 2. The Km value of the enzyme was 7.81, however the Km values effected by SaGunJa extracts of 0.1% and 1% were decreased to 5.44 and 3.85 respectively. 3. The energy of activation of the control was 16.2 ㎉/mole, and the values were decreased to 15.4 ㎉/mole and 14.88 ㎉/mole in the presence of 0.1% and 1% SGJex respectively.

Luria-Nebraska 神經心理檢査의 韓國版 標準化硏究(Ⅱ)

金在煥,南正鉉,金光日,金二泳,黃淳澤,김지혜,金南奎,朴康圭,林東洙,金圭泰 漢陽大學校 精神健康硏究 1990 精神健康硏究 Vol.9 No.-

동종 골수이식 후 만성 이식편대숙주질환과 동반되어 신증후군으로 발현된 IgA 신병증 1예

정철권,김현수,박준성,정성현,조도연,최진혁,남동기,임호영,김명성,신규태,임현이,김효철 대한조혈모세포이식학회 1999 대한조혈모세포이식학회지 Vol.4 No.2

동종 골수이식 후 발생하는 신기능부전은 원인이 다양하여 감별에 어려움이 있다. 만성 GVHD는 흉선의 기능저하에 따른 면역관용의 실패로 발생되는 자가면역현상에 의해 발생되는 것으로 알려져 있으며 드물게 신장을 침범하여 신증후군을 일으키기도 한다. CaA의 용량을 줄이거나 투약중지 후 발생한 만성 GVHD에 동반된 신증후군의 경우 조기에 신조직검사를 시행하여 감별진단하고 CsA의 용량을 증량하거나 재투약하여 좋은 치료 결과를 보일 것으로 기대된다. 저자들은 문헌고찰을 통해 동종 골수이식 후 발생된 신증후군이 유사한 임상양상을 보이며 발생기전에 만성 GVHD와 자가면역현상이 연관되어 있음을 확인하였으며 동종 골수이식 후 신증후군의 임상양상을 보이는 환자에서 병리조직학적으로 lgA 신병증을 진단하고 이러한 lgA 신병증이 발생기전에 만성 GVHD와 자가 면역현상이 연관되어 있을 가능성을 최초로 확인하였으며 CsA를 이용하여 성공적으로 치료한 경험을 하였기에 보고하는 바이다. Renal insufficiency is occasionally encountered in allogeneic bone marrow transplantation and its cause is difficult to ascertain. Chronic graft-versus-host disease (GVHD) related to thymic dysfunction is immune-mediated and involves autoreactivity of T-lymphocytes derived from donor marrow to recipient's major histocompatibility complex(MHC) minor antigens. The clinical mainifestations of chronic GVHD are similar to those of autoimmune disease but kidney involvement is rare. Few cases of nephrotic syndrome with membranous nephropathy or minimal change nephrotic syndrome have been reported to be associated with chronic GVHD in allogeneic bone marrow transplant recipient especially after cyclosporine A(CsA) withdrawal, and these cases have responded well to CsA. Therefore, it is prudent to differentiate the cause of post-transplant renal insufficiency using renal biopsy and to start CsA in a case of nephrotic syndrome associated with chronic GVHD as early as possible. We report a case who had a massive proteinuria during the post-allogeneic marrow transplantation period. The cause of nephrotic syndrome was diagnosed as IgA nephropathy by renal biopsy. The patient was successfully treated with reintroduction of CsA.

Understanding of Relation Gas Transport Properties with Free Volume Elements in Fluorinated Polyimide Membranes

Nam Gyu Lim,Hyo Won Kim 한국막학회 2023 한국막학회 총회 및 학술발표회 Vol.2023 No.05

Comparison of Anti-Inflammatory Effects of Flavonoid-Rich Common and Tartary Buckwheat Sprout Extracts in Lipopolysaccharide-Stimulated RAW 264.7 and Peritoneal Macrophages

Nam, Tae Gyu,Lim, Tae-Gyu,Lee, Bong Han,Lim, Sol,Kang, Hee,Eom, Seok Hyun,Yoo, Miyoung,Jang, Hae Won,Kim, Dae-Ok Hindawi 2017 Oxidative medicine and cellular longevity Vol.2017 No.-

<P>Buckwheat sprouts have been widely consumed all around world due to their great abundance of bioactive compounds. In this study, the anti-inflammatory effects of flavonoid-rich common buckwheat sprout (CBS) and tartary buckwheat sprout (TBS) extracts were evaluated in lipopolysaccharide- (LPS-) stimulated RAW 264.7 murine macrophages and primary peritoneal macrophages from male BALB/c mice. Based on the reversed-phase HPLC analysis, the major flavonoids in CBS were determined to be <I>C</I>-glycosylflavones (orientin, isoorientin, vitexin, and isovitexin), quercetin-3-<I>O</I>-robinobioside, and rutin, whereas TBS contained only high amounts of rutin. The TBS extract exhibited higher inhibitory activity as assessed by the production of proinflammatory mediators such as nitric oxide and cytokines including tumor necrosis factor-<I>α</I>, interleukin- (IL-) 6, and IL-12 in LPS-stimulated RAW 264.7 macrophages than CBS extract. In addition, TBS extract suppressed nuclear factor-kappa B activation by preventing inhibitor kappa B-alpha degradation and mitogen-activated protein kinase phosphorylation in LPS-stimulated RAW 264.7 macrophages. Moreover, the TBS extract markedly reduced LPS-induced cytokine production in peritoneal macrophages. Taken together, these findings suggest that TBS extract can be a potential source of anti-inflammatory agents that may influence macrophage-mediated inflammatory disorders.</P>

Right side fixation of sigmoid colon with a hepato-sigmoidocolic fistula in patient with hepatocellular carcinoma and midgut malrotation

Nam Gyu Choi,Ok In Moon,Jin Ha Kim,Sharon Lim,Sung Chul Lim,Jun Lee,Kyung Jong Kim 대한외과학회 2013 Annals of Surgical Treatment and Research(ASRT) Vol.84 No.4

The location of the sigmoid colon varies within the abdominal cavity, but its mesocolon is fixed to the left side. Right side fixation of the sigmoid colon is a very rare congenital positional anomaly. In addition, it has been reported that hepatocolic fistula is also a very rare disease that may present lower gastrointestinal bleeding. Here, the authors describe a case of a 71-year-old man who underwent surgery for hepato-sigmoidocolic fistula complicated by hepatocellular carcinoma and the right side fixation of the sigmoid colon.

Engineering Gas Separation Properties in Fluorinated Polyimide Membranes via Free Volume Engineering

Nam Gyu Lim,Jae Sung Park,Jae Gi Lee,Hyo Won Kim 한국막학회 2023 한국막학회 총회 및 학술발표회 Vol.2023 No.11

20-O-β-D-glucopyranosyl-20(S)-protopanaxadiol, a metabolite of ginsenoside Rb1, enhances the production of hyaluronic acid through the activation of ERK and Akt mediated by Src tyrosin kinase in human keratinocytes.

Lim, Tae-Gyu,Jeon, Ae Ji,Yoon, Ji Hye,Song, Dasom,Kim, Jong-Eun,Kwon, Jung Yeon,Kim, Jong Rhan,Kang, Nam Joo,Park, Jun-Seong,Yeom, Myeong Hun,Oh, Deok-Kun,Lim, Yoongho,Lee, Charles C,Lee, Chang Yong,L UNKNOWN 2015 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Vol.35 No.5

<P>The aim of the present study was to determine the mechanisms through which 20-O-beta-D-glucopyranosy120(S)-protopanaxadiol (20GPPD) promotes the production of hyaluronic acid (HA) in human keratinocytes. 20GPPD is the primary bioactive metabolite of Rb1, a major ginsenoside found in ginseng (Panax ginseng). We sought to elucidate the underlying mechanisms behind the 20GPPD-induced production of HA. We found that 20GPPD induced an increase in HA production by elevating hyaluronan synthase 2 (HAS2) expression in human keratinocytes. The phosphorylation of extracellular signal-regulated kinase (ERK) and Akt was also enhanced by 20GPPD in a dose-dependent manner. The pharmacological inhibition of ERK (using U0126) or Akt (using LY294002) suppressed the 20GPPD-induced expression of HAS2, whereas treatment with an epidermal growth factor receptor (EGFR) inhibitor (AG1478) or an intracellular Ca2+ chelator (BAPTA/AM) did not exert any observable effects. The increased Src phosphorylation was also confirmed following treatment with 20GPPD in the human keratinocytes. Following pre-treatment with the Src inhibitor, PP2, both HA production and HAS2 expression were attenuated. Furthermore, the 20GPPD-enhanced ERK and Akt signaling decreased following treatment with PP2. Taken together, our results suggest that Src kinase plays a critical role in the 20GPPD-induced production of HA by acting as an upstream modulator of ERK and Akt activity in human keratinocytes.</P>

Quercetin과 Rutin을 함유하는 PCL-b-PEG 고분자 미셀의 특성 및 피부 흡수에 관한 In vitro 연구

임규남(Gyu Nam Lim),김선영(Sun Young Kim),김민지(Min Ji Kim),박수남(Soo Nam Park) 한국고분자학회 2012 폴리머 Vol.36 No.4

본 연구에서는 항산화 물질로 잘 알려진 quercetin과 그 배당체인 rutin을 함유하는 poly(ε-caprolactone)-bpoly (ethylene glycol) 미셀을 제조하여, 활성물질(quercetin, rutin)의 in vitro 피부 흡수 증진에 관한 연구를 수행하였다. 입자크기는 PCL-b-PEG 고분자의 농도가 증가함에 따라 미셀의 초기 입자 크기가 증가하는 경향을 보였다. 고 분자 미셀의 표면 전위(Zeta potential)는 비교적 일정함을 확인하였다. 제조한 고분자 미셀의 피부 흡수력을 평가하기 위하여, 용액 상태의 활성물질을 미셀의 대조군으로 하여 Franz cell을 이용한 투과실험을 진행한 결과 용액 상 태보다 미셀에서 더 높게 나타났음을 확인하였다. 또한 화장품 소재로서의 안전성 평가를 위한 인체 피부 일차자극 실험(patch test) 결과 어떠한 피부 자극도 관찰되지 않았다. In this study, we prepared polymer micelles containing quercetin and rutin, known as antioxidants, using poly (ε-caprolactone)-b-poly (ethylene glycol), and evaluated in vitro skin permeation of the active materials. Quercetin and rutin loaded micelles were characterized by DSC (differential scanning calorimetry), HPLC (high performance liquid chromatography) and DLS (dynamic light scattering) measurements. The particle size of the polymer micelles increased in a concentration dependent manner (0.5~2.0% PCL-b-PEG). The Zeta potential of quercetin and rutin loaded micelles remained constant. To evaluate the skin penetration of PCL-b-PEG micelles, Franz diffusion cell experiment was performed. The aqueous solutions of quercetin and rutin were used as the control groups. Quercetin and rutin loaded PCL-b- PEG micelles showed more efficient skin permeation than the control groups. Safety assessment (patch test) of quercetin and rutin loaded PCL-b-PEG micelles on skin was performed to test application possibility of the polymer micelles to cosmetics. Any adverse symptoms were not observed.