난포기 및 황체기에 있어서 돼지 난관 협부 평활근의 neurotransmitter들의 수축효과에 대한 estradiol-17β및 progesterone의 영향

이민기,노규진,심철수,남윤정,김주현,최상용 慶尙大學校 1991 論文集 Vol.30 No.2

The effects of estradiol-17β or progesterone on the contractility of neurotransmitters on pig oviductal isthmic smooth muscle were investigated. The motility of the isolated smooth muscle was recorded by using physiological recording system. The results were summarized as follows : 1. The contractility induced by histamine or prostaglandin F2α was depressed by the pretreatment of estradiol-17β but was not by the preteatment of progesterone in follicular or luteal stage. 2. The contractility induced by acetylcholine was depressed un follicular stage but excited in luteal stage by the pretreatment of estradiol-17β or progesterone. 3. The contractility induced by norepinephrine was depressed by the pretreatment of progesterone in follicular stage but was excited by the pretreatment estradiol-17β in luteal stage.

In Vitro and In Vivo Differentiation of Porcine Ad비t Stem Cells Derived from Skin, Adipose and Ovarian Tissues into Oocyte-like Cells

Gyu Jin Rho 한국동물생명공학회(구 한국동물번식학회) 2010 Reproductive & developmental biology Vol.34 No.2

Enhanced development of porcine embryos cloned from bone marrow mesenchymal stem cells

Jin, Hai-feng,Kumar, B Mohana,Kim, Jung-Gon,Song, Hye-Jin,Jeong, Yeon-Ji,Cho, Seong-Keun,Balasubramanian, Sivasankaran,Choe, Sang-Yong,Rho, Gyu-Jin UPV/EHU Press 2007 The international journal of developmental biology Vol.51 No.1

<P>In the present study, we have characterized an isolated population of porcine bone marrow mesenchymal stem cells (MSCs) for multilineage commitment and compared the developmental potential of cloned embryos with porcine MSCs and fetal fibroblasts (FFs). MSCs exhibited robust alkaline phosphatase activity and later transformed into mineralized nodules following osteoinduction. Furthermore, MSCs underwent adipogenic and chondrogenic differentiation by producing lipid droplets and proteoglycans, respectively. Primary cultures of FFs from a female fetus at ~30 day of gestation were established. Donor cells at 3-4 passage were employed for nuclear transfer (NT). Cell cycle analysis showed that the majority of MSCs in confluence were in the G0/G1 stage. Cumulus-oocyte complexes were matured and fertilized in vitro (IVF) as control. The cleavage rate was significantly (P<0.05) higher in IVF than in NT embryos with MSCs and FFs (84.54.6% vs. 52.25.4% and 50.85.2%, respectively). However, blastocyst rates in IVF and NT embryos derived from MSCs (20.62.5% and 18.43.0%) did not differ, but were significantly (P<0.05) higher than NT derived from FFs (9.52.1%). Total cell number and the ratio of ICM to total cells among blastocysts cloned from MSCs (34.45.2 and 0.380.08, respectively) were significantly (P<0.05) higher than those from FFs (22.65.5 and 0.180.12, respectively). Proportions of TUNEL positive cells in NT embryos from FFs (7.31.8%) were significantly (P<0.05) higher than in MSCs (4.61.3%) and IVF (2.50.9%). The results clearly demonstrate that multipotent bone marrow MSCs have a greater potential as donor cells than FFs in achieving enhanced production of cloned porcine embryos.</P>

In Vitro and In Vivo Differentiation of Porcine Ad비t Stem Cells Derived from Skin, Adipose and Ovarian Tissues into Oocyte-like Cells

Gyu Jin Rho 한국동물번식학회 2010 Reproductive & Developmental Biology(Supplement) Vol.34 No.2s

Human Umbilical Cord Blood-Derived CD34-Positive Endothelial Progenitor Cells Stimulate Osteoblastic Differentiation of Cultured Human Periosteal-Derived Osteoblasts

Lee, Jin-Ho,Hah, Young-Sool,Cho, Hee-Young,Kim, Jin-Hyun,Oh, Se-Heang,Park, Bong-Wook,Kang, Young-Hoon,Choi, Mun-Jeoung,Shin, Jeong-Kyu,Rho, Gyu-Jin,Jeon, Ryoung-Hoon,Lee, Hee-Chun,Kim, Gyoo-Cheon,Kim Mary Ann Liebert 2014 Tissue engineering. Part A Vol.20 No.5

Comparisons of Development Potential in Bovine SCNT Embryos using Donor Cells treated with Different Demethylating Inhibitors

Byeong-Gyun Jeon,Gie-Joon Jeong,Gyu-Jin Rho 한국수정란이식학회 2015 한국동물생명공학회지 Vol.30 No.3

To improve the developmental potential of bovine somatic cell nuclear transfer (SCNT) embryos, this study compared the developmental rates to blastocyst stage in the SCNT embryos using donor fibroblasts treated with 5-azacytidine (5AC) and S-adenosylhomocysteine (SAH) at different concentrations. Their reprogramming efficiency level was investigated with level of telomerase activity. Donor fibroblasts isolated from adult ear skin of a cow were exposed to 5AC and SAH at different concentrations during 2 passages. After nuclear transfer into enucleated recipient oocytes, the cleavage and developmental rates were significantly (p<0.05) decreased in the SCNT embryos using 5AC-treated fibroblasts (5AC-SCNT embryos), compared with those of non-treated control (control-SCNT embryos) and SAH-treated fibroblasts (SAH-SCNT embryos). The developmental rates to blastocyst stage tended to be slightly increased in the SAH-SCNT embryos at each of the concentrations, and especially, the developmental rates in the SCNT embryos using 1.0 mM SAH-treated fibroblasts were significantly (p<0.05) higher than that of control SCNT embryos. The mean numbers of total and ICM cell in blastocysts were also significantly (p<0.05) decreased in the 5AC-SCNT embryos, compared with those of other SCNT blastocysts. Further, the level of telomerase activity was also significantly (p< 0.05) decreased in the 5AC-SCNT embryos than those of control and SAH-SCNT embryos. Whereas, a significantly (p<0.05) up-regulated telomerase activity was observed in SAH-SCNT embryos, compare with that of control-SCNT embryos. In conclusion, SCNT embryos using hypomethylated donor cells with SAH, not 5AC, may improve the developmental potential and reprogramming efficiency.

Comparison of Developmental Competency of Porcine Embryos Cloned with Mesenchymal Stem Cells and Somatic Cells

Jin Hai-Feng,Kumar B. Mohana,Cho Sung-Keun,Ock Sun-A,Jeon Byeong-Gyun,Balasubramanian S.,Choe Sang-Yong,Rho Gyu-Jin The Korean Society of Animal Reproduction 2006 Reproductive & developmental biology Vol.30 No.2

The present study compared the developmental potential of cloned porcine embryos with mesenchymal stem cells (MSCs), fetal fibroblasts (FFs) and cumulus cells (CCs) by assessing the cleavage and blastocyst rate, total cell number, inner cell mass (ICM) ratio and apoptosis. MSCs were isolated by ficoll gradients from femur of -6 month old female pig, and maintained for primary cultures. FFs from a female fetus at ${\sim}30$ day of gestation were established, and CCs were obtained from cumulus oocyte complexes (COCs) aspirated from $3{\sim}6$ mm follicles in diameter. Donor cells at $3{\sim}4$ passage were employed for nuclear transfer (NT). COCs were matured and fertilized in vitro(IVF) as control. Cleavage rate was significantly (P<0.05) higher in IVF than in NT embryos with MSCs, FFs and CCs ($82.7{\pm}8.9%\;vs\;70.6{\pm}5.4,\;68.7{\pm}5.1\;and\;63.4{\pm}5.6%$, respectively). However, blastocyst rates in IVF and NT embryos derived from MSCs ($24.5{\pm}2.8\;and\;20.4{\pm}8.3%$) did not differ, but were significantly (P<0.05) higher than NT derived from FFs and CCs ($10.6{\pm}2.7\;and\;9.8{\pm}2.1%$). Total cell number and the ratio of ICM to total cells among blastocysts cloned from MSCs ($35.4{\pm}5.2\;and\;0.40{\pm}0.09%$, respectively) were significantly (P<0.05) higher than those from FFs and CCs ($24.9{\pm}6.2%\;vs\;0.19{\pm}0.16,\;23.6{\pm}5.5\;and\;0.17{\pm}0.16%$, respectively). Proportions of TUNEL positive cells in NT embryos from FFs and CCs ($6.9{\pm}1.5\;and\;7.4{\pm}1.7%$, respectively) were significantly (P<0.05) higher than in MSCs ($4.8{\pm}1.4%$) and IVF ($2.3{\pm}0.9%$). The results demonstrate that MSCs have a greater potential as donor cells than FFs and CCs in achieving enhanced production of cloned porcine embryos.

액정셀의 전기광학적 편광투과특성을 이용한 액정표시소자의 최적설계 : Ⅰ. 단색광의 경우 Ⅰ. Monochromatic Case

노봉규,김규석,김진승 全北大學校 基礎科學硏究所 1994 基礎科學 Vol.17 No.-

액정층을 지나는 빛의 편광상태의 변화를 뽀앙카레공에서의 회전변환으로 나타내어, 액정층에 걸어준 전압에 대한 회전변환의 회전각과 회전축의 변화를 쟀다. 이로부터 액정셀에 걸린 전압이 비선택전압에서 선택전압으로 바뀔 때의 회전변환의 회전각이 180˚에 가장 가까운 구동전압을 결정하고, 이 전압으로 액정셀을 구동할 때 화면의 콘트라스트 및 투과율이 최대가 되는 평광판과 검광판의 투과축의 방향을 회전변환의 일반적인 특성을 써서 결정하고, 실험적으로 확인하였다. The electro-optic polarization transmission characteristics of a liquid crystal (LC) cell is represented by a rotational transformation on the Poincare sphere, and its characteristic parameters, the angle and the axis of the rotation, are experimentally determined with respect to the voltage applied to the LC cell. From the experimental data obtained, we determine driving voltage for the LC cell give the rotation angle near 180˚ of the rotation for the change of the state of the LC cell from non-select to select state, which is a necessary condition for the realization of maximally bright images. The directions of the transmission axes of the polarizer and the analyzer which form a liquid crystal display panel together with the LC cell, are determined from the general characteristics of the rotational transformation of the LC cell.

The Prevalence and Awareness of Hypertension and the Relationship between Hypertension and Snoring in the Korean Population

(Jin Seok Kim),(Woo Hyuk Song),(Chol Shin),(Chang Gyu Park),(Hong Seog Seo),(Wan Joo Shim),(Dong Joo Oh),(Sae Hwa Ryu),(Young Moo Rho) 대한내과학회 2001 The Korean Journal of Internal Medicine Vol.16 No.2

N/A Background: Hypertension is the most important, and yet modifiable, risk factor for cardiovascular diseases. But in many countries, hypertension remains poorly controlled. Moreover, sleep apnea syndrome has shown that it is correlated with hypertension. The purpose of this study was to investigate the prevalence, awareness and control of hypertension among the Korean people and to evaluate the relationship between hypertension and snoring. Methods: A total of 640 subjects living in Ansan, a regional city in Korea, were selected randomly, and trained nursing students investigated their age, sex, medical history, blood pressure, body mass index (BMI) and snoring score. Blood pressures were measured three times with a 10 - minute interval and then averaged. The degree of snoring was estimated using a questionnaire. We divided the subjects into hypertensive (BP≥140/90 mmHg) versus normotensive group and snorer versus non-snorer group, and correlated hypertension with snoring. Results: Of 640 subjects, 311 were male. The mean age was 39.7±14.6 years (18-77 years), the mean BMI (body mass index) was 22.4±3.0 kg/m2. The mean systolic and diastolic blood pressure was 121±15.7 mmHg and 79.5±11.6 mmHg. The prevalence and awareness of hypertension were 22.2% and 16.9%, respectively, and the prevalence of snoring was 35.2%. With the increment of age, in the male, the prevalence of hypertension and snoring were higher, and the snorer group showed a higher risk of hypertension than the non-snorer group (Odds ratio 2.32, CI=1.56-3.39, p=0.0001). Conclusion: In Korea, the prevalence of hypertension was similar to that in the western countries, but the awareness of hypertension was much lower compared with western countries. The prevalence of hypertension was higher in the snorer group, so more research on the correlation between the two conditions should be advanced in the future.

유니타리 존즈행렬의 기하학적 해석과 벡터표현

노봉규,김진승 全北大學校 基礎科學硏究所 1994 基礎科學 Vol.17 No.-

투명한 비등방성 매질의 편광투과특성을 나타내는 유니타리 존즈행렬과 뽀앙카레공의 표면에서의 회전변환이 일대일 대응되는 것을 보여주는 공식을 끌어내었다. 이 공식들을 쓰면 유니타리 존즈행렬의 세 매개변수로부터 이에 대응되는 회전변환의 벡터표현으로부터 이에 대응되는 유니타리 존즈행렬의 매개변수를 결정할 수 있다. 빛이 투명한 비등방성 선형매질을 지날 때 편광상태의 변화를 살펴보려면 먼저 매질전체의 편광투과특성을 나타내는 존즈행렬을 계산하고, 이로부터 뽀앙카레공에서의 회전변환을 결정하여 뽀앙카레공 위의 점들이 어떻게 회전이동하는가 보면 된다. We derive a set of formulae which show one-to-one correspondence between the the unitary Jones matrices of transparent anisotropic media and the rotational transformations on the Poincare sphere. By using the formulae one can determine the vectorial representation of the rotational transformation on the Poincare sphere which specifies the direction of the axis and the angle of the rotation in terms of the three parameters specific to the corresponding unitary Jones matrix, and conversely the the three parameters of the unitary Jones matrix in terms of the vectorial representation of the corresponding rotational transformation on the Poincare sphere. To understand the polarization transmission characteristics of an optical system consisting of transparent linear anisotropic media, start with the Jones calculus to get the unitary Jones matrix for the whole system and then convert it to a rotational transformation on the Poincare sphere, from which we can intuitively understand the effect of the optical system on the polarization state of the light passing through the system.