Influence of Kluyveromyces marxianus on proteins, peptides, and amino acids in Lactobacillus-fermented milk

Dong-Dong Zhang,Jing-Lan Liu,Tie-Min Jiang,Lu Li,Guo-Zhen Fang,Yan-Pin Liu,Li-Jun Chen 한국식품과학회 2017 Food Science and Biotechnology Vol.26 No.3

With increasing application of yeast in fermented milk, in order to study the effect of yeast on milk protein during the fermentation process, the effects of the presence of Kluyveromyces marxianus in milk fermented by Streptococcus thermophilus and Lactobacillus bulgaricus were investigated. After fermentation, the amino acid, protein, and peptide contents were analyzed by ultra-performance liquid chromatography, two-dimensional gel electrophoresis, and liquid chromatography–mass spectrometry, respectively. After the addition of K. marxianus for fermentation, 25 protein spots changed significantly. These were mostly caseins and bovine serum proteins, and the content of total free amino acids increased by 16.30%; ten types of bioactive peptides were identified. Furthermore, the number of peptide types in milk fermented by K. marxianus increased significantly compared with milk fermented by Lactobacillus. K. marxianus is considered to promote proteometabolism in milk when added with Lactobacillus, generate flavor compounds, and improve the digestion and absorption character of milk.

Comparative transcriptomic and physiological analyses of contrasting hybrid cultivars ND476 and ZX978 identify important differentially expressed genes and pathways regulating drought stress tolerance in maize

Guo Liu,Tinashe Zenda,Songtao Liu,Xuan Wang,Hongyu Jin,Anyi Dong,Yatong Yang,Huijun Duan 한국유전학회 2020 Genes & Genomics Vol.42 No.8

Background Drought is the major abiotic stress factor that negatively influences growth and yield in cereal grain crops such as maize (Zea mays L.). A multitude of genes and pathways tightly modulate plant growth, development and responses to environmental stresses including drought. Therefore, crop breeding efforts for enhanced drought resistance require improved knowledge of plant drought responses. Objective Here, we sought to elucidate the molecular and physiological mechanisms underpinning maize drought stress tolerance. Methods We therefore applied a 12-day water-deficit stress treatment to maize plants of two contrasting (drought tolerant ND476 and drought sensitive ZX978) hybrid cultivars at the late vegetative (V12) growth stage and performed a large-scale RNA sequencing (RNA-seq) transcriptome analysis of the leaf tissues. Results A comparative analysis of the two genotypes leaf transcriptomes and physiological parameters revealed the key differentially expressed genes (DEGs) and metabolic pathways that respond to drought in a genotype-specific manner. A total of 3114 DEGs were identified, with 21 DEGs being specifically expressed in tolerant genotype ND476 in response to drought stress. Of these, genes involved in secondary metabolites biosynthesis, transcription factor regulation, detoxification and stress defense were highly expressed in ND476. Physiological analysis results substantiated our RNA-seq data, with ND476 exhibiting better cell water retention, higher soluble protein content and guaiacol peroxidase activity, along with low lipid peroxidation extent than the sensitive cultivar ZX978 under drought conditions. Conclusion Our findings enrich the maize genetic resources and enhance our further understanding of the molecular mechanisms regulating drought stress tolerance in maize. Additionally, the DEGs screened in this study may provide a foundational basis for our future targeted cloning studies.

Mechanical evaluation of polymer microneedles for transdermal drug delivery: In vitro and in vivo

Rui Xuan Liu,Yu Ting He,Ling Liang,Liu Fu Hu,Yue Liu,Rui-xing Yu,Bo Zhi Chen,Yong Cui,Xin Dong Guo 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.114 No.-

In this study, we reported two types of PMNs based on polylactic acid (PLA) and polyvinyl alcohol (PVA),respectively. Parafilm M film, porcine skin, and rats’ models were operated to evaluate the mechanicalproperties in vitro and in vivo to find optimal parameters for efficient insertion. Insertion depth was measuredusing Digital Force Gauge by changing insertion force and speed, respectively. Results showed thatincreasing the insertion force and speed used for PMNs application led to a significant increase in thedepth of insertion. A force of 18 N under a speed of 330 mm/min was the optimal condition for insertingPMNs array into ParafilmM film and porcine skin. In addition, PLA-MNs exhibited higher robustness andenhanced homogeneity in insertion depth compared with PVA-MNs, but PVA-MNs were able to reachmuch deeper insertion depth. Moreover, Sprague Dawley (SD) rat experiments confirmed the effectivenessof optimal insertion parameters for transdermal drug delivery. This study illustrated not only thedevelopment of novel PMNs but also the mechanical evaluation for the design of PMNs.

Research and Application of MAS

Guo Ruifeng,Yu Dong,Liu Minglie 대한전자공학회 1998 ICEIC:International Conference on Electronics, Inf Vol.1 No.1

Inhibition of Nemo-like Kinase Increases Taxol Sensitivity in Laryngeal Cancer

Dong, Ji-Rui,Guo, Nan,Zhao, Jian-Pu,Liu, Pin-Duo,Feng, Hui-Hong,Li, Yan Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.12

Several studies have shown that nemo-like kinase (NLK) plays a vital role in apoptosis of cancer cells. The present research concerned effects and mechanisms of Taxol on NLK knockdown human laryngeal cancerHep-2 cell lines in vitro. Using RNAi, methyl-thiazoltetrazolium (MTT) assays, real-time RT-PCR, Western blotting and flow cytometry analysis, growth and the cell cycle progression of NLK knockdown Hep-2 cells and expression of downstream molecules were observed. Cell growth was obviously suppressed in the Taxol treated group (P<0.001, 48 hours). Cell numbers of combined Taxol-based chemotherapy with lentivirus mediated RNAi treatment group (Lv-shNLK+Taxol goup) were significantly different from NLK-specific siRNA lentivirus infected group (Lv-shNLK group) (p<0.001). Flow cytometry analysis revealed that Lv-shNLK+Taxol caused the G0/G1-phase DNA content to decrease from 44.1 to 3.33% (p<0.001) and the S-phase DNA content to increase from 38.4 to 82.0% (p<0.001), in comparison with the Lv-shNLK+Taxol group. Immunoblot analysis showed that knockdown of NLK led to significant reduction in the levels of cyclin D1, PCNA and PARP, whereas cyclin B1 was elevated in. Cell growth was also obviously suppressed in the Hep-2 cell line, knockdown of NLK making them more sensitive to Taxol treatment. NLK is expected to become a target of new laryngeal cancer gene therapies.

Molecular Orbital Gating Surface-Enhanced Raman Scattering

Guo, Chenyang,Chen, Xing,Ding, Song-Yuan,Mayer, Dirk,Wang, Qingling,Zhao, Zhikai,Ni, Lifa,Liu, Haitao,Lee, Takhee,Xu, Bingqian,Xiang, Dong American Chemical Society 2018 ACS NANO Vol.12 No.11

<P>One of the promising approaches to meet the urgent demand for further device miniaturization is to create functional devices using single molecules. Although various single-molecule electronic devices have been demonstrated recently, single-molecule optical devices which use external stimulations to control the optical response of a single molecule have rarely been reported. Here, we propose and demonstrate a field-effect Raman scattering (FERS) device with a single molecule, an optical counterpart to field-effect transistors (a key component of modern electronics). With our devices, the gap size between electrodes can be precisely adjusted at subangstrom accuracy to form single molecular junctions as well as to reach the maximum performance of Raman scattering via plasmonic enhancement. Based on this maximum performance, we demonstrated that the intensity of Raman scattering can be further enhanced by an additional ∼40% if the orbitals of the molecules bridged two electrodes were shifted by a gating voltage. This finding not only provides a method to increase the sensitivity of Raman scattering beyond the limit of plasmonic enhancement, but also makes it feasible to realize addressable functional FERS devices with a gate electrode array.</P> [FIG OMISSION]</BR>

Accuracy of the Different Calculation Methods of Specific Edge Load

LIU Huan,DONG Jixian,LUO Chong,DUAN Chuanwu,GUO Xiya,QI Kai,QIAO Lijie,ZHAO Zhiming 한국펄프·종이공학회 2020 펄프.종이기술 Vol.52 No.5

Modification of DC Flashover Voltage at High Altitude on the Basis of Molecular Gas Dynamics

Dong-Ming Liu,Fu-Sheng Guo,Wen-xia Sima 대한전기학회 2015 Journal of Electrical Engineering & Technology Vol.10 No.2

The effect of altitude on thermal conduction, surface temperature, and thermal radiation of partial arc was investigated on the basis of molecular gas dynamics to facilitate a deep understanding of the pollution surface discharge mechanism. The DC flashover model was consequently modified at high altitude. The validity of the modified DC flashover model proposed in this paper was proven through a comparison with the results of high-altitude simulation experiments and earlier models. Moreover, the modified model was found to be better than the earlier modified models in terms of forecasting the flashover voltage. Findings indicated that both the thermal conduction coefficient and the surface thermodynamics temperature of partial arc had a linear decrease tendency with the altitude increasing from 0 m to 3000 m, both of which dropped by approximately 30% and 3.6%, respectively. Meanwhile, the heat conduction and the heat radiation of partial arc both had a similar linear decrease of approximately 15%. The maximum error of DC pollution flashover voltage between the calculation value according to the modified model and the experimental value was within 6.6%, and the pollution flashover voltage exhibited a parabola downtrend with increasing of pollution.

The transcription factor FoxM1 activates Nurr1 to promote intestinal regeneration after ischemia/ reperfusion injury

Guo Zu,Jing Guo,Tingting Zhou,Ningwei Che,Baiying Liu,Dong Wang,Xiangwen Zhang 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-

FoxM1 is involved in the regeneration of several organs after injury and expressed in the intestinal mucosa. The intrinsic mechanism of FoxM1 activity in the mucosa after intestinal ischemia/reperfusion (I/R) injury has not been reported. Therefore, we investigated the role of FoxM1 in mediating intestinal mucosa regeneration after I/R injury. Expression of FoxM1 and the proliferation of intestinal mucosa epithelial cells were examined in rats with intestinal I/R injury and an IEC-6 cell hypoxia/reperfusion (H/R) model. The effects of FoxM1 inhibition or activation on intestinal epithelial cell proliferation were measured. FoxM1 expression was consistent with the proliferation of intestinal epithelial cells in the intestinal mucosa after I/R injury. Inhibition of FoxM1 expression led to the downregulation of Ki67 expression mediated by the inhibited expression of Nurr1, and FoxM1 overexpression promoted IEC-6 cell proliferation after H/R injury through activating Nurr1 expression. Furthermore, FoxM1 directly promoted the transcription of Nurr1 by directly binding the promoter of Nurr1. Further investigation showed low expression levels of FoxM1, Nurr1, and Ki-67 in the intestinal epithelium of patients with intestinal ischemic injury. FoxM1 acts as a critical regulator of intestinal regeneration after I/R injury by directly promoting the transcription of Nurr1. The FoxM1/ Nurr1 signaling pathway represents a promising therapeutic target for intestinal I/R injury and related clinical diseases.

Modification of DC Flashover Voltage at High Altitude on the Basis of Molecular Gas Dynamics

Liu, Dong-Ming,Guo, Fu-Sheng,Sima, Wen-Xia The Korean Institute of Electrical Engineers 2015 Journal of Electrical Engineering & Technology Vol.10 No.2

The effect of altitude on thermal conduction, surface temperature, and thermal radiation of partial arc was investigated on the basis of molecular gas dynamics to facilitate a deep understanding of the pollution surface discharge mechanism. The DC flashover model was consequently modified at high altitude. The validity of the modified DC flashover model proposed in this paper was proven through a comparison with the results of high-altitude simulation experiments and earlier models. Moreover, the modified model was found to be better than the earlier modified models in terms of forecasting the flashover voltage. Findings indicated that both the thermal conduction coefficient and the surface thermodynamics temperature of partial arc had a linear decrease tendency with the altitude increasing from 0 m to 3000 m, both of which dropped by approximately 30% and 3.6%, respectively. Meanwhile, the heat conduction and the heat radiation of partial arc both had a similar linear decrease of approximately 15%. The maximum error of DC pollution flashover voltage between the calculation value according to the modified model and the experimental value was within 6.6%, and the pollution flashover voltage exhibited a parabola downtrend with increasing of pollution.