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        The Cement Content Measurement of Cement Mixing Piles with EDTA Titration Method

        Fuhai Zhang,Lei Zhang,Liguo Yang,Tugen Feng,Lei Chen,Xiaochun Zhong 대한토목학회 2018 KSCE Journal of Civil Engineering Vol.22 No.11

        The uniformity of cement mixing piles is visually tested on the drilled core samples, significantly relying on experience and extremely inaccurate. The cement content of core samples is an important parameter to quantify the uniformity of cement mixing piles. However, there were no well-accepted methods of testing the cement content of core samples. EDTA titration method is widely used as an effective way to test the cement content of cement-stabilized soil in highway subgrade. However, the cement mixing piles cannot be drilled until 28 days after construction, which causes remarkable change of water content and cement hydration during this period. In addition, factors affecting the EDTA titration results should be investigated before standard EDTA titration curves are constructed. In this paper, effects of different factors including curing method, curing period, soil type, content of water glass, and cement type, on EDTA titration testing results were investigated. It is found that cement type and soil type will significantly affect the test result. It is critical to keep the curing environment and soil unsieved before testing. Water glass will reduce the EDTA solution consumptions at early stages of curing. The EDTA titration results are verified by the proposed density estimation method.

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        Aerosol Measurement and Property Analysis Based on Data Collected by a Micro-pulse LIDAR over Shanghai, China

        Xingyou Huang,Xiaowu Yang,Fuhai Geng,Hua Zhang,Qianshan He,Lingbing Bu 한국광학회 2010 Current Optics and Photonics Vol.14 No.3

        A micro-pulse LIDAR system (MPL) was employed to measure the aerosol over Pudong, Shanghai from July 2008 to January 2009. Based on Fernald method, aerosol optical variables such as extinction coefficient were retrieved and analyzed. Results show that aerosol exists mainly in low layers; aerosol loading reaches its maximum in the afternoon, and then decreases with time until its minimum at night. Most of the aerosol concentrates in the layer below 3 km, and optical extinction coefficient in the layer below 2 km contributes 84.25% of that below 6 km. Two extinction coefficient peaks appear in the near surface layer up to 500 m and in the level around 1000 m. Aerosol extinction coefficient shows a seasonal downward trend from summer to winter.

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        Nanoarchitectonics of tannic acid based injectable hydrogel regulate the microglial phenotype to enhance neuroplasticity for poststroke rehabilitation

        Zongjian Liu,Shulei Zhang,Yuanyuan Ran,Huimin Geng,Fuhai Gao,Guiqin Tian,Zengguo Feng,Jianing Xi,Lin Ye,Wei Su 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00

        Background Stroke is the second leading cause of mortality and disability worldwide. Poststroke rehabilitation is still unsatisfactory in clinics, which brings great pain and economic burdens to stroke patients. In this study, an injectable hydrogel in which tannic acid (TA) acts as not only a building block but also a therapeutic drug, was developed for poststroke rehabilitation. Methods TA is used as a building block to form an injectable hydrogel (TA gel) with carboxymethyl chitosan (CMCS) by multivalent hydrogen bonds. The morphology, rheological properties, and TA release behavior of the hydrogel were characterized. The abilities of the TA gel to modulate microglial (BV2 cells) polarization and subsequently enhance the neuroplasticity of neuro cells (N2a cells) were assessed in vitro. The TA gel was injected into the cavity of stroke mice to evaluate motor function recovery, microglial polarization, and neuroplasticity in vivo. The molecular pathway through which TA modulates microglial polarization was also explored both in vitro and in vivo. Results The TA gel exhibited sustainable release behavior of TA. The TA gel can suppress the expression of CD16 and IL-1β, and upregulate the expression of CD206 and TGF-β in oxygen and glucose-deprived (OGD) BV2 cells, indicating the regulation of OGD BV2 cells to an anti-inflammatory phenotype in vitro. This finding further shows that the decrease in synaptophysin and PSD95 in OGD N2a cells is effectively recovered by anti-inflammatory BV2 cells. Furthermore, the TA gel decreased CD16/iNOS expression and increased CD206 expression in the periinfarct area of stroke mice, implying anti-inflammatory polarization of microglia in vivo. The colocalization of PSD95 and Vglut1 stains, as well as Golgi staining, showed the enhancement of neuroplasticity by the TA gel. Spontaneously, the TA gel successfully recovered the motor function of stroke mice. The western blot results in vitro and in vivo suggested that the TA gel regulated microglial polarization via the NF-κB pathway. Conclusion The TA gel serves as an effective brain injectable implant to treat stroke and shows promising potential to promote poststroke rehabilitation in the clinic.

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