Transcriptional response of Methoprene-tolerant (Met) gene to three insect growth disruptors in Leptinotarsa decemlineata (Say)

Qing-Wei Meng,Qing-Yu Xu,Pan Deng,Kai-Yun Fu,Wen-Chao Guo,Guo-Qing Li 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.2

Some insect growth disruptors (IGDs), such as pyriproxyfen and halofenozide, may be used to control Leptinotarsa decemlineata. However, their mechanism of action remains elusive. Methoprene-tolerant (Met) mediates juvenile hormone (JH) signal to control numerous essential physiological processes. In the present paper, we identified a Met gene (LdMet). LdMet protein was a typical basic helix-loop-helix/Per-Arnt-Sim (bHLHPAS) transcription factor with a bHLH domain, two PAS domains (PAS-A and PAS-B) and a region called PAS associated C terminal (PAC). Eight conserved amino acids critical for JH binding were located in PAS-B and PAC domains. The temporal expression pattern of LdMet was in accordance with the variation of circulating JH titers. Feeding of juvenoid methoprene or pyriproxyfen, or provide for JH dose-dependently stimulated the expression of LdMet. RNA interference-mediated knockdown of two JH degradation genes increased the transcription of LdMet, while silencing of a JH biosynthesis gene repressed the transcription. Conversely, ingestion of an ecdysteroid agonist halofenozide or 20-hydroxyecdysone (20E) reduced the mRNA levels of LdMet, in a dosedependent manner, whereas knockdown of either ecdysteroidogenesis or 20E signaling genes increased the mRNA accumulation. Providing that the expression of LdMet can be disturbed by methoprene, pyriproxyfen and halofenozide, LdMet may be a potential target of these IGDs in L. decemlineata larvae.

Control of Electrically Excited Synchronous Motors with a Low Switching Frequency

Qing-qing Yuan,Xiao-jie Wu,Peng Dai,Xiao Fu 전력전자학회 2012 JOURNAL OF POWER ELECTRONICS Vol.12 No.4

The switching frequency of the power electronic devices used in large synchronous motor drives is usually kept low (less than 1 kHz) to reduce the switching losses and to improve the converter power capability. However, this results in a couple of problems, e.g. an increase in the harmonic components of the stator current, and an undesired cross-coupling between the magnetization current component (im) and the torque component (it). In this paper, a novel complex matrix model of electrically excited synchronous motors (EESM) was established with a new control scheme for coping with the low switching frequency issues. First, a hybrid observer was proposed to identify the instantaneous fundamental component of the stator current, which results in an obvious reduction of both the total harmonic distortion (THD) and the low order harmonics. Then, a novel complex current controller was designed to realize the decoupling between im and it. Simulation and experimental results verify the effectiveness of this novel control system for EESM drives.

ATM NETWORK MANAGEMENT IN CHINA

Qing Fu Hou,Lu Ming Chen 대한전자공학회 1998 대한전자공학회 워크샵 Vol.1998 No.-

Plant genetic resources in China and their application to plant biotechnology

Qing Fu Chen 한국작물학회 2007 한국작물학회 학술발표대회 논문집 Vol.2007 No.11

Plant germplasm resources are the plant materials containing useful inheritable characters of actual or potential value. Generally speaking, plant resources include cultivated and wild plants. China is a megadiversity country. A great lot of different complex climate environments in China such as dryness, wetness, clearness, clouding, shade, plain, hill, mountain, and so on, have gestated many Chinese endemic special plants. Some of them have special genes, such as resistance to drought, chilliness, disease, pests, and saltness etc, elite quality, high yield characteristics and so on. There are 470 families、about 3 7000 genus、more than 30 000 species of lichen, fern、seed plants in China. Special species in China involve 200 genus and about 10 000 species. Plant resources in China are conservated in situ and ex situ, by means of reforestation, regrassing, natural protection zones, arboreta, campus, parks, and institutes, etc. National Crop Gene Bank in Beijing, built in 1986, has 3200m2, two long-term rooms (T=-18℃±1℃, RH<50%) with the capability 400,000. There are more than 340,000 accessions in long-term banks of the National Crop Gene Bank of China, involving 35 families, 192 genus, 725 species. Among them, 80% are native to China. Up to now, there have been more than 210 main tree species for artificial forestation in China. Among them, there are more than 120 species for reforestation of barren slopes, deserts, and stony mountains. There are about 800-1000 Chinese traditional medicine plants, which were mainly conservated in situ and ex situ. The application of plant germplasm in China in plant biotechnology were reviewed on the following aspects: (1)Core germplasm construction by molecular marker technology (2)Plant germplasm preservation by biotechnology (3) Plant germplasm reproduction and industrialization by biotechnology (4) Elite gene discoverfrom plant germplasm by biotechnology (5) Plant germplasm in China and gene engineering technology. At last, some problems of plant resources in China were put forward.

High Efficiency Photovoltaic Pump System with Double Pumps

Fu Qing,Chen Xiaogao,Xie Lei 전력전자학회 2007 ICPE(ISPE)논문집 Vol.- No.-

The efficiency of Photovoltaic Pump System (PVPS) varies with the solar irradiation and it is important to select the suitable pump to improve the efficiency of PVPS. Pump with higher head works longer but is in lower efficiency when solar irradiation is powerful in noon. The paper proposes a Maximum Power Trace (MPPT) controller for PVPS and a novel scheme with double pumps to improve efficiency of PVPS. The pump with higher head works when solar irradiation is weak while the pump with lower head is automatically switched to work when solar irradiation is powerful. The experiment proves the higher efficiency of PVPS with double pumps.

Fracture property of steel fiber reinforced concrete at early age

Chuan-Qing Fu,Qin-yong Ma,Xianyu Jin,A.A. Shah,Ye Tian 사단법인 한국계산역학회 2014 Computers and Concrete, An International Journal Vol.13 No.1

This research is focused on obtaining the fracture property of steel fiber reinforced concrete(SFRC) specimens at early ages of 1, 2, 3 and 7-day, respectively. For this purpose, three point bending tests of nine groups of SFRC beams with notch of 40mm depth and different steel fiber ratios were conducted. The experimental results of early age specimens were compared with the 28-day hardened SFRC specimens. The test results indicated that the steel fiber ratios and curing age significantly influenced the fracture properties of SFRC. A reasonable addition of steel fiber improved the fracture toughness of SFRC, while the fracture energy of SFRC developed with curing age. Moreover, a quadratic relationship between splitting strength and fracture toughness was established based on the experiment results. Additionally, afinite element (FE) method was used to investigate the fracture properties of SFRC.A comparison between the FE analysis and experiment results was also made. The numerical analysis fitted well with the test results, and further details on the failure behaviors of SFRC could be revealed by the suggested numerical simulation method.

Three new diphenylpropanes from Celastrus hindsii

Xian Qing Hu,Wei Han,Zhu Zhen Han,Qing Xin Liu,Xi-Ke Xu,Peng Fu,Hui-Liang Li 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.11

Three new diphenylpropanes, HindsiipropaneA–C (1–3), together with one known arylpropyl quinoneGriffithane D (4), were isolated from Celastrus hindsii. Their structures were established by 1D and 2D nuclearmagnetic resonance spectroscopic analysis, and massspectroscopy. Compound 4 was firstly obtained in thisgenus. All the isolated compounds were evaluated in vitrofor cytotoxicity against four human tumor cell lines (A549,HCT116, MDA-MB-231, BEL7404) by the MTT assay.

Control of Electrically Excited Synchronous Motors with a Low Switching Frequency

Yuan, Qing-Qing,Wu, Xiao-Jie,Dai, Peng,Fu, Xiao The Korean Institute of Power Electronics 2012 JOURNAL OF POWER ELECTRONICS Vol.12 No.4

The switching frequency of the power electronic devices used in large synchronous motor drives is usually kept low (less than 1 kHz) to reduce the switching losses and to improve the converter power capability. However, this results in a couple of problems, e.g. an increase in the harmonic components of the stator current, and an undesired cross-coupling between the magnetization current component ($i_m$) and the torque component ($i_t$). In this paper, a novel complex matrix model of electrically excited synchronous motors (EESM) was established with a new control scheme for coping with the low switching frequency issues. First, a hybrid observer was proposed to identify the instantaneous fundamental component of the stator current, which results in an obvious reduction of both the total harmonic distortion (THD) and the low order harmonics. Then, a novel complex current controller was designed to realize the decoupling between $i_m$ and $i_t$. Simulation and experimental results verify the effectiveness of this novel control system for EESM drives.

MAGED4 Expression in Glioma and Upregulation in Glioma Cell Lines with 5-Aza-2'-Deoxycytidine Treatment

Zhang, Qing-Mei,Shen, Ning,Xie, Sha,Bi, Shui-Qing,Luo, Bin,Lin, Yong-Da,Fu, Jun,Zhou, Su-Fang,Luo, Guo-Rong,Xie, Xiao-Xun,Xiao, Shao-Wen Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.8

Melanoma-associated antigen (MAGE) family genes have been considered as potentially promising targets for anticancer immunotherapy. MAGED4 was originally identified as a glioma-specific antigen. Current knowledge about MAGED4 expression in glioma is only based on mRNA analysis and MAGED4 protein expression has not been elucidated. In the present study, we investigated this point and found that MAGED4 mRNA and protein were absent or very lowly expressed in various normal tissues and glioma cell line SHG44, but overexpressed in glioma cell lines A172,U251,U87-MG as well as glioma tissues, with significant heterogeneity. Furthermore, MAGED4 protein expression was positively correlated with the glioma type and grade. We also found that the expression of MAGED4 inversely correlated with the overall methylation status of the MAGED4 promoter CpG island. Furthermore, when SHG44 and A172 with higher methylation were treated with the DNA demethylating agent 5-aza-2'-deoxycytidine (5-AZA-CdR) reactivation of MAGED4 mRNA was mediated by significant demethylation in SHG44 instead of A172. However, 5-AZA-CdR treatment had no effect on MAGED4 protein in both SHG44 and A172 cells. In conclusion, MAGED4 is frequently and highly expressed in glioma and is partly regulated by DNA methylation. The results suggest that MAGED4 might be a promising target for glioma immunotherapy combined with 5-AZA-CdR to enhance its expression and eliminate intratumor heterogeneity.

MFCC 기반의 햅틱 질감 인식 및 분류

한경복(Qing-Fu Han),김형국(Hyoung-Gook Kim) 대한전자공학회 2015 대한전자공학회 학술대회 Vol.2015 No.6