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Kim, Ji Yeon,Hwang, Joo-Yeon,Lee, Dae Yeon,Song, Eun Hyun,Park, Keon Jae,Kim, Gyu Hee,Jeong, Eun Ae,Lee, Yoo Jeong,Go, Min Jin,Kim, Dae Jin,Lee, Seong Su,Kim, Bong-Jo,Song, Jihyun,Roh, Gu Seob,Gao, Bi American Society for Biochemistry and Molecular Bi 2014 The Journal of biological chemistry Vol.289 No.39
<▼1><P><B>Background:</B> Chronic ethanol consumption induces pancreatic β-cell dysfunction and metabolic syndrome.</P><P><B>Results:</B> Ethanol-induced Atf3 inhibits glucokinase transcriptional activity through direct binding or Atf3/Pdx-1/Hdac1 axis on glucokinase promoter.</P><P><B>Conclusion:</B> ATf3 fosters β-cell dysfunction via <I>Gck</I> down-regulation and triggers T2D, which is ameliorated by <I>in vivo Atf3</I> silencing.</P><P><B>Significance:</B> The presented data uncover a new role for Atf3 as a potential therapeutic target in treating type 2 diabetes.</P></▼1><▼2><P>Chronic ethanol consumption induces pancreatic β-cell dysfunction through glucokinase (Gck) nitration and down-regulation, leading to impaired glucose tolerance and insulin resistance, but the underlying mechanism remains largely unknown. Here, we demonstrate that <I>Gck</I> gene expression and promoter activity in pancreatic β-cells were suppressed by chronic ethanol exposure <I>in vivo</I> and <I>in vitro</I>, whereas expression of activating transcription factor 3 (Atf3) and its binding to the putative Atf/Creb site (from −287 to −158 bp) on the <I>Gck</I> promoter were up-regulated. Furthermore, <I>in vitro</I> ethanol-induced Atf3 inhibited the positive effect of Pdx-1 on <I>Gck</I> transcriptional regulation, enhanced recruitment of Hdac1/2 and histone H3 deacetylation, and subsequently augmented the interaction of Hdac1/Pdx-1 on the <I>Gck</I> promoter, which were diminished by <I>Atf3</I> siRNA. <I>In vivo Atf3</I>-silencing reversed ethanol-mediated <I>Gck</I> down-regulation and β-cell dysfunction, followed by the amelioration of impaired glucose tolerance and insulin resistance. Together, we identified that ethanol-induced <I>Atf3</I> fosters β-cell dysfunction via <I>Gck</I> down-regulation and that its loss ameliorates metabolic syndrome and could be a potential therapeutic target in treating type 2 diabetes. The <I>Atf3</I> gene is associated with the induction of type 2 diabetes and alcohol consumption-induced metabolic impairment and thus may be the major negative regulator for glucose homeostasis.</P></▼2>
Eun-Bi Park(박은비),Hye-Sun Kim(김혜선),So-Yun Shin(신소연),In-Ae Ji(지인해),Ji-Hyun Kim(김지현),Sung-Goo Kim(김성구),Byung Hong Yoo(유병홍),Byung-Woo Kim(김병우),Inseok Kwak(곽인석),Moon-Moo Kim(김문무),Kyung Tae Chung(정경태) 한국생명과학회 2012 생명과학회지 Vol.22 No.11
산수유는 다양한 생리적 기능을 가지는 것으로 보고되어 있으며 또한 최근에 관심의 증가로 산수유로부터 유효성분의 추출 방법 개발은 산업적으로 매우 중요하며, 유효성분의 존재 여부를 분석하는 것은 산업적 상품의 진위와 품질을 판정하는데 중요한 수단이 된다. 본 연구는 loganin을 지표 물질로 하여 산수유로부터 유효성분을 추출하는 여러 방법을 비교 분석하였으며, 각 방법에 따라 추출한 시료의 기능성의 차이를 항산화 작용으로 분석하였다. 산수유로부터 항산화 성분을 추출하기 위하여 네 가지 방법을 적용하였다. 추출된 유효성분 지표물질로 loganin의 함량을 HPLC로 분석하였을 때 열수를 사용한 추출 방법이 알코올 추출 또는 효소 처리 방법 보다효율적인 결과를 보여 주었다. 각 추출 시료의 항산화 기능은 DPPH radical 소거능, hydrogen peroxide scavenging assay, reducing power 측정으로 분석하였다. 추출 방법에 따라 항산화 기능이 차이가 있었으며, 열수 추출 시료가 DPPH radical 소거능과 hydrogen peroxide scavenging 효과가 우수하였고, 효소 처리 추출 시료는 reducing power가 우수하게 나타났다. 추출 방법에 따라 시료에 함유된 성분의 차이가 항산화 기능이 차이와 연관이 있다고 생각된다. 이 연구에서 얻은 결과는 산수유는 대조군으로 사용한 vitamin C에 준하는 항산화 성분을 함유하고 있으며, 이들 성분은 열수 추출 방법으로 효율적으로 추출되었으며, 열수 추출 방법은 산업적으로 응용 될 수 있음을 제시하고 있다. Oxidative stress leads to damage in all components of the cell, including proteins, lipids, and DNA due to imbalance between reactive oxygen species production and cellular detoxification. Phytochemicals are well-known to contain antioxidants, and their physiological role has been intensively studied. The fruit of Cornus officianalis has been used in oriental medicine and has been reported to have many functions. In this study, four different extraction techniques were applied to extract functional components from the fruit of Cornus officianalis, and the content of loganin, which is an antioxidant having DPPH radical and hydrogen peroxide scavenging activity and reducing power, was analyzed in each extract. Extraction techniques employed in this study were heat extraction by water, 70% ethanol extraction, enzyme treatment, and combination of enzyme treatment and heat extraction by water. All extracts contained 11.8-18.0 mg/g loganin and showed antioxidation function assayed by measuring DPPH radical and hydrogen peroxide scavenging activity and reducing power. Among them, heat extraction was the most effective technique, showing a maximum amount of loganin (18.0 mg/g) and antioxidative activity at 100 mg/ml concentration. Each extract showed very low cytotoxicity up to at 500 mg/ml but 10-20% cytotoxicity at 1,000 mg/ml by in vitro MTT assay.
김은비(Eun Bi Kim),전태웅(Eun Bi Kim),한창희(Chang Hee Han) 한국산업경영시스템학회 2012 한국산업경영시스템학회지 Vol.35 No.2
In the manufacturing industry, fostering talented workers has become a core activity as well as an important resource leading to manufacturing enterprises' innovative activities, which has come to the fore as one of the biggest success factors for innovative activities indeed. In other countries, there are many researches actively conducted on talent-fostering programs related to manufacture innovation. In Korea, however, manufacturers or several different education agencies don't actively carry out manufacture innovation instructions and have failed to faster special competent workers since their instructions focus on theories most of the time. Therefore, this study was conducted to understand the present conditions of manufacture-innovative instructions in Korea by classifying them in a method of educational technology and to investigate various teaching methods through oversea cases and cases from non-manufacture fields so that it might make a proposal for a better manufacture-innovative instruction method.
Kim, Hyun‐,Ouk,Kim, Eunjung,An, Yonghee,Choi, Jihye,Jang, Eunji,Choi, Eun Bi,Kukreja, Aastha,Kim, Myeong‐,Hoon,Kang, Byunghoon,Kim, Dong‐,Joo,Suh, Jin‐,Suck,Huh, Yong‐,Mi WILEY‐VCH Verlag 2013 Macromolecular bioscience Vol.13 No.6
<P><B>Abstract</B></P><P>Combined cancer treatment via co‐delivery of siRNAs and an anticancer drug can be a promising strategy due to the synergistic effect of simultaneously minimizing gene/drug administration. In this study, Bcl‐xL siRNA and doxorubicin (DOX) are encapsulated into designed methoxy‐poly(ethylene glycol)‐<I>block</I>‐poly(<SMALL>D</SMALL>,<SMALL>L</SMALL>‐lactic acid) (mPEG‐<I>b</I>‐PLA) block copolymer polymersomes (PSomes). A study of the cytotoxicity of Bcl‐xL siRNA and DOX co‐encapsulated PSomes (CPSomes) shows more inhibited proliferation of MKN‐45 and MKN‐28 human gastric cancer cell lines than only gene‐ and drug‐loaded ones. Consequently, these results demonstrate that co‐delivery of genes and drugs using PSomes results in a synergistic efficacy and indicates the potential of PSomes as efficient nanocarriers for combined cancer therapy. </P>