초고온 호기성 퇴비화 공정의 복합 악취 및 악취 물질 평가

박세용(Park Seyong),정대혁(Jung Dai-Hyuck),유의상(Yoo Eui-Sang),김문일(Kim Moonil) 한국지반환경공학회 2009 한국지반환경공학회논문집 Vol.10 No.5

본 연구는 정읍시에서 배출되는 하수 슬러지, 축산 분뇨, 음식물 쓰레기를 대상으로 Pilot Scale(100㎥) 초고온 호기성 퇴비화 공정에서의 복합 악취 및 12개 지정 악취물질을 평가하였다. 각각의 대상 물질은 종균과 혼합하였고 1차 50일, 2차 60일 기간동안 운전하였다. 호기성 퇴비화가 진행됨에 따라 1차, 2차 퇴비화공정에서 온도가 상승하여 약 90~95℃가 되었다. 악취 분석을 위한 시료는 1차의 경우 퇴비상부에서, 2차의 경우 Pilot Plant 경계지점에서 채취하였다. 지정 악취 물질 12개 항목의 기기 분석에서 악취의 주요 원인으로는 암모니아, 메틸머캅탄, 디메틸다이설파이드, 트리메틸아민이었다. 초기 복합악취의 농도는 퇴비화 공정이 끝난 후의 농도보다 높았지만 규제기준을 넘지 않았다. 초고온 호기성 퇴비화 공정에서는 적절한 교반시점과 발효 온도가 악취 발생의 중요한 인자라고 판단된다. This study was conducted to evaluate production of complex odor and 12 specific odorous compounds in a pilot-scale (capacity : 100 ㎥) ultra thermophilic aerobic composting. There were three types input: municipal wasted sludge, livestock manure and slurry, and food waste produced in Jung-Eb city. Each raw material was mixed with seed material and operated for two periods (1st : 50 days, 2nd : 60days). During composting, the temperature hit 90~95℃ after every mixing in both periods. Therefore, it was concluded that increasing temperature also saves the time which required for composting and high reduction of organics and water contents. The primary odorous compounds were ammonia, methyl mercaltan, dimethyl disulfide and trimethylamine. The concentration of the primary compounds and complex odor during the operation were higher than those on final day and most compounds did not exceed the allowable exhaust standard for odor. Also, it was found that optimal mixing time and control of high temperature are the most important parameters for odor control in ultra thermophilic aerobic composting.

Establishment of inducible cAMP early repressor transgenic fibroblasts in a porcine model of human type 1 diabetes mellitus.

Jung, Eui-Man,Kim, Yu-Kyung,Lee, Geun-Shik,Hyun, Sang-Hwan,Hwang, Woo-Suk,Jeung, Eui-Bae D. A. Spandidos 2012 MOLECULAR MEDICINE REPORTS Vol.6 No.1

<P>Diabetes mellitus is a metabolic disease caused by impaired insulin secretion from the pancreatic β cells and increased insulin resistance in peripheral tissues. Recently, the overexpression of inducible cyclic AMP (cAMP) early repressor (ICER) Iγ in rodent pancreatic β cells was found to induce insulin deficiency and glucagon overproduction similar to that found in human diabetes mellitus. ICER Iγ with only a DNA binding domain interrupts the transcriptional regulation of the cAMP responsive element-binding protein (CREB) target genes. Based on this information, we hypothesized that the overexpression of ICER Iγ, the most powerful competitor to CREB, could be useful for generating a pig model of diabetes. First, we evaluated the promoter activities of the human insulin gene for the β cell-specific overexpression of ICER Iγ in the pig pancreas. The maximum promoter activity region [-1,431 nucleotides (nt) to +1 nt, +1 = the transcriptional start site] of the insulin gene presented an activity level 3-fold higher than a promoterless construct. Second, ICER Iγ overexpression controlled by this promoter region significantly blocked the glucose-mediated insulin transcription, such as that regulated by the viral promoter in the pancreatic β?cell line, MIN6. This suggests that the human insulin promoter may facilitate the overexpression of ICER?Iγ in porcine pancreatic β cells. In addition, the overexpression of ICER?Iγ in porcine β cells may induce human-like type 1 diabetes mellitus in pigs. In the present study, we generated transgenic fibroblasts containing ICER Iγ cDNA controlled by the human insulin promoter, as well as two screening markers, the green fluorescence protein and the neomycin resistance gene. These fibroblasts may provide a source for somatic cell nuclear transfer to generate a pig model that mimics human diabetes mellitus.</P>

Adult xanthogranuloma: A clinical, pathological, and immunohistochemical study of 19 Korean cases

( Sang-woo Park ),( Eui-sung Jung ),( Hyun-bin Kwak ),( Soo-kyung Park ),( Kyung-hwa Nam ),( Jin Park ),( Seok-kweon Yun ),( Han-uk Kim ) 대한피부과학회 2018 대한피부과학회 학술발표대회집 Vol.70 No.2

Background: Juvenile xanthogranuloma is a benign, self-limited disorder that usually occurs in infants and young children. However, it is rare in adults and there is a lack of study on adult xanthogranuloma. Objectives: To investigate the clinical, pathological, and immunohistochemical characteristics of adult xanthogranuloma. Methods: This study included 20 lesions from 19 patients with adult xanthogranuloma. Results: There was a male predilection (M:F=1.4:1), and mean age was 35.1±16.3 years (range 15~66 years), with the peak in the twenties. 65.0% of the lesions developed on the head and neck. The nodular form was more common than the papular form. Histological examination in 5 lesions (25.0%) revealed dense monomorphic histiocytic infiltration without lipidization and scattered eosinophils. There was also absence of multinuclear giant cells. 10 lesions (50.0%) had foamy histiocytic infiltration with variations of fully developed Touton giant cells. 3 lesions (15.0%) showed fibrohistiocytic proliferation. Histiocytes, including giant cells, were generally positive for FXIIIa (90.9%), vimentin (100%) and CD68 (100%). The CD1a, SMA and S-100 protein reactions were all negative. Tumor excision was the treatment for choice. Conclusion: This retrospective study has a small sample size and is limited to a single center, but we expect the results of this study to contribute to better understanding adult xanthogranuloma.

Establishment of transgenic fibroblasts for producing recombinant human interferon-α and erythropoietin in bovine milk.

Jung, Eui-Man,An, Beum-Soo,Kim, Yu-Kyung,Hwang, Inho,Lee, Jong-Yun,Shin, Tae-Young,Hyun, Sang-Hwan,Hwang, Woo-Suk,Jeung, Eui-Bae D. A. Spandidos 2013 MOLECULAR MEDICINE REPORTS Vol.7 No.2

<P>Human interferon α (IFN-α) and erythropoietin (EPO) have been used for a variety of purposes in clinical medicine. Human IFN-α has been used to treat several types of viral infection and cancer, as well as renal anemia, via stimulation of erythrocyte formation in the bone marrow. Transgenic cattle are excellent candidates for pharmaceutical production for humans due to their ability to produce recombinant proteins in milk. The purpose of the present study was to generate bovine transgenic fibroblasts capable of producing recombinant human IFN-α and EPO proteins in transgenic cattle milk. First, we analyzed the promoter activities of various bovine milk protein genes in HC11 mouse mammary epithelial cells. The bovine milk protein gene promoters were cloned into the Luc gene in a promoter-less pGL3-Basic vector. Presence of the αS1-casein promoter (-175 to +796 nt) resulted in an up to 16-fold increase in luciferase activity compared with that of the promoter-less construct. In addition, the human IFN-α and EPO genes were identified as significantly overexpressed in HC11 cells compared with the promoter-less construct. Together, the present results demonstrate that the construct with the αS1-casein promoter may induce secretion of recombinant human IFN-α and EPO into bovine milk. Furthermore, we generated transgenic fibroblasts expressing human IFN-α and EPO cDNA controlled by the αS1-casein promoter and two screening markers, enhanced green fluorescent protein and neomycin resistance. These transgenic fibroblasts may be a source of somatic cells for generating transgenic cattle that produce recombinant human IFN-α and EPO proteins during lactation.</P>

Pressure alopecia caused by an orthodontic headgear appliance

( Eui-sung Jung ),( Su-kyung Park ),( Seok-kweon Yun ),( Han-uk Kim ),( Jin Park ) 대한피부과학회 2018 대한피부과학회 학술발표대회집 Vol.70 No.1

Pressure alopecia is a kind of localized hair loss, resulting from excessive or chronic local pressure of the scalp. It is usually described in patients with immobilization of the head during longstanding surgery or in bed-ridden state; however, it can rarely occur from a long-term use of various fixation instruments including head straps, orthodontic headgears, hairpins, and even wigs. A 9-year-old female presented with a localized hair loss patch on the frontal scalp after putting on a headgear appliance for several days due to her orthodontic treatment. She was complaining of pruritus, bruising and oozing on the area where direct pressure was applied by the headgear. Physical examination showed a 3.5 x 3.5 cm, round bald patch and another 2.0 x 3.0 cm, oval bald patch on the frontal scalp. Dermoscopy revealed scattered yellow and black dots with some broken hairs and without any exclamation mark hairs, which is the characteristics of alopecia areata. Histopathologic findings revealed trichomalacia with pigmented cast, and increased number of catagen and telogen hair follicles, but with no peribulbar infiltration. Based on clinical and histologic findings, he was diagnosed as pressure alopecia due to orthodontic headgear. After 3 weeks of removal of the headgear, newly emerging hairs was observed on the lesion. During the 4 months of follow-up period, complete hair regrowth was noted.

Epidermal inclusion cyst of the umbilicus in a child

( Sang-woo Park ),( Eui-sung Jung ),( Hyun-bin Kwak ),( Soo-kyung Park ),( Seok-kweon Yun ),( Han-uk Kim ),( Jin Park ) 대한피부과학회 2018 대한피부과학회 학술발표대회집 Vol.70 No.1

Epidermal cyst is a common benign cutaneous tumor that predominantly occurs on the face, trunk, and neck, where hair follicles are present. The umbilicus is one of the rarest sites of epidermal cyst, and only a few umbilical epidermal cysts have been reported, primarily in adults. Herein, we report a rare case of umbilical epidermal cyst in a child, which should be included in the differential diagnosis of umbilical skin lesions. A 7-year-old girl visited us for an asymptomatic protruding nodule on her umbilicus that had gradually increased in size over three years. Physical examination showed a 0.8 cm × 0.8 cm pearly gray, spherical nodule in the umbilicus. Dermoscopy revealed shiny white structures with arborizing vessels on the surface of the nodule. Ultrasonography revealed a well-circumscribed cyst confined to the skin layer, but tubular structures penetrating into the abdominal cavity or connecting to internal organs were not seen. Histopathologic findings of the tissue from complete excision of the mass showed a cyst that is lined with stratified squamous epithelium with granular layer and containing keratins. No complication or recurrence was observed during the 6 months after tumor removal.

Isoprene Production in Escherichia coli

Jung-Hun KIM,Sang-Hwal YOON,Chong-Long WANG,Asad Ali SHAH,Hee-Jeong JANG,Jong-Wook SONG,Seo-Hee KANG,Hee-Kyoung RYU,Eui-Sung CHOI,Seon-Won KIM 한국생물공학회 2009 한국생물공학회 학술대회 Vol.2009 No.10

Comparative Evaluation of Medication Compliance in Accordance with the Method of Medication Teaching in Community Pharmacies

Sung Han Yoon,Hyun Taek Shin,Jung Mi Oh,Hea Kyoung Cho,Eui Kyoung Lee,Suk Hyang Lee 한국임상약학회 2002 학술대회 Vol.2002 No.1

Tankyrase-mediated β-catenin activity regulates vasopressin-induced AQP2 expression in kidney collecting duct mpkCCDc14 cells

Jung, Hyun Jun,Kim, Sang-Yeob,Choi, Hyo-Jung,Park, Eui-Jung,Lim, Jung-Suk,Frøkiaer, Jørgen,Nielsen, Søren,Kwon, Tae-Hwan American Physiological Society 2015 American journal of physiology. Renal physiology Vol.308 No.5

<P>Aquaporin-2 (AQP2) mediates arginine vasopressin (AVP)-induced water reabsorption in the kidney collecting duct. AVP regulates AQP2 expression primarily via G(s)alpha/cAMP/PKA signaling. Tankyrase, a member of the poly(ADP-ribose) polymerase family, is known to mediate Wnt/beta-catenin signaling-induced gene expression. We examined whether tankyrase plays a role in AVP-induced AQP2 regulation via ADP-ribosylation of G protein-alpha (G alpha) and/or beta-catenin-mediated transcription of AQP2. RT-PCR and immunoblotting analysis revealed the mRNA and protein expression of tankyrase in mouse kidney and mouse collecting duct mpkCCDc14 cells. dDAVP-induced AQP2 upregulation was attenuated in mpkCCDc14 cells under the tankyrase inhibition by XAV939 treatment or small interfering (si) RNA knockdown. Fluorescence resonance energy transfer image analysis, however, revealed that XAV939 treatment did not affect dDAVP-or forskolin-induced PKA activation. Inhibition of tankyrase decreased dDAVP-induced phosphorylation of beta-catenin (S552) and nuclear translocation of phospho-beta-catenin. siRNA-mediated knockdown of beta-catenin decreased forskolin-induced AQP2 transcription and dDAVP-induced AQP2 expression. Moreover, inhibition of phosphoinositide 3-kinase/Akt, which was associated with decreased nuclear translocation of beta-catenin, diminished dDAVP-induced AQP2 upregulation, further indicating that beta-catenin mediates AQP2 expression. Taken together, tankyrase plays a role in AVP-induced AQP2 regulation, which is likely via beta-catenin-mediated transcription of AQP2, but not ADP-ribosylation of G alpha. The results provide novel insights into vasopressin-mediated urine concentration and homeostasis of body water metabolism.</P>

Implementation of DMA-based FIR Filtering for Implantable Middle Ear Hearing Devices using MSP430 Microprocessor

Eui-Sung Jung,Jong-Min Kim,Yong-Ho Yoon,Sung-Hoon Kim,Seung-Ha Lee,Il-Yong Park,Jin-Ho Cho 대한전자공학회 2006 ITC-CSCC :International Technical Conference on Ci Vol.2006 No.3