Calcium Sensitization Induced by Sodium Fluoride in Permeabilized Rat Mesenteric Arteries

Enyue Yang,Joon Yong Cho,Uy Dong Sohn,In Kyeom Kim 대한생리학회-대한약리학회 2010 The Korean Journal of Physiology & Pharmacology Vol.14 No.1

It was hypothesized that NaF induces calcium sensitization in Ca²⁺-controlled solution in permeabilized rat mesenteric arteries. Rat mesenteric arteries were permeabilized with Ղ- escin and subjected to tension measurement. NaF potentiated the concentration-response curves to Ca²⁺ (decreased EC₅₀ and increased E_max). Cumulative addition of NaF (4.0, 8.0 and 16 mM) also increased vascular tension in Ca²⁺-controlled solution at pCa 7.0 or pCa 6.5, but not at pCa 8.0. NaF-induced vasocontraction and GTPՃS-induced vasocontraction were not additive. NaF-induced vasocontraction at pCa 7.0 was inhibited by pretreatment with Rho kinase inhibitors H1152 or Y27632 but not with a MLCK inhibitor ML-7 or a PKC inhibitor Ro31-8220. NaF induces calcium sensitization in a Ca²⁺- dependent manner in Ղ-escin-permeabilized rat mesenteric arteries. These results suggest that NaF is an activator of the Rho kinase signaling pathway during vascular contraction.

17β-Estradiol Attenuates Vascular Contraction Through Inhibition Of RhoA/Rho Kinase Signaling Pathway

Yang, Enyue,Jeon, Su Bun,Baek, Inji,Song, Min-Ji,Kim, In Kyeom Elsevier 2009 Biophysical journal Vol.96 No.3

Intelligent Virtual Impedance Based Control to Enhance the Stability of Islanded Microgrid

Jiang Enyu,Zhao Jikang,Shi Zhengjing,Mi Yang,Lin Shunfu,Muyeen S. M. 대한전기학회 2023 Journal of Electrical Engineering & Technology Vol.18 No.5

This paper focuses on the voltage stability issue of an islanded microgrid in a cost-effective way adding the concept of adaptive virtual impedance. In the islanded microgrid structure, the mis-match of line impedance between the Distributed Generation (DG) units and imbalance of inverter local load are two critical factors to be dealt with carefully. These can result in serious circulating current and unreasonably reactive power sharing among DG units by using the conventional droop control method. In practical engineering, line inductance is often increased to eliminate the impedance mismatch. However, such a method would lead to an increase in cost, weight, and volume. To solve this problem, an adaptive virtual impedance droop control strategy based on fuzzy controller is proposed. Instead of DG units are equipped with series inductors, an adaptive virtual impedance is introduced to adaptively eliminate the reactive power error caused by the inconsistency of the line impedance through the fuzzy controller. At the same time, in order to deal with the droop control and the voltage drop at the inverter output caused by the addition of virtual impedance, a voltage compensation link is introduced to maintain the voltage within an acceptable range. The simulations were performed in Matlab/Simulink and RT-LAB experiments to verify the validity of the proposed control strategy.

Two Compound Heterozygous Were Identified in SLC26A4 Gene in Two Chinese Families With Enlarged Vestibular Aqueduct

Yongbo Yu,Yang Yang,Jie Lu,Yaqiong Jin,Yeran Yang,Enyu Hong,Jin Shi,Feng Chen,Shujing Han,Ping Chu,Yongli Guo,Xin Ni 대한이비인후과학회 2019 Clinical and Experimental Otorhinolaryngology Vol.12 No.1

Objectives. To investigate the genetic causes of hearing loss with enlarged vestibular aqueduct (EVA) in two children from unrelated two Chinese families. Methods. Sanger sequencing of all coding exons in SLC26A4 (encoding Pendrin protein) was performed on the two patients, their sibling and parents respectively. To predict and visualize the potential functional outcome of the novel variant, model building, structure analysis, and in silico analysis were further conducted. Results. The results showed that the proband from family I harbored a compound heterozygote of SLC26A4 c.1174A>T (p.N392Y) mutation and c.1181delTCT (p.F394del) variant in exon 10, potentially altering Pendrin protein structure. In family II, the proband was identified in compound heterozygosity with a known mutation of c.919-2A>G in the splice site of intron 7 and a novel mutation of c.1023insC in exon 9, which results in a frameshift and translational termination, consequently leading to truncated Pendrin protein. Sequence homology analysis indicated that all the mutations localized at high conservation sites, which emphasized the significance of these mutations on Pendrin spatial organization and function. Conclusion. In summary, this study revealed two compound heterozygous mutations (c.1174A>T/c.1181delTCT; c.919-2A>G/c.1023insC) in Pendrin protein, which might account for the deafness of the two probands clinically diagnosed with EVA. Thus this study contributes to improve understanding of the causes of hearing loss associated with EVA and develop a more scientific screening strategy for deafness.

A functional polymorphism on chromosome 15q25 associated with survival of early stage non-small-cell lung cancer.

Jin, Guang,Bae, Eun Young,Yang, Enyue,Lee, Eung Bae,Lee, Won-Kee,Choi, Jin Eun,Jeon, Hyo-Sung,Yoo, Seung Soo,Lee, Shin Yup,Lee, Jaehee,Cha, Sung Ick,Kim, Chang Ho,Cho, Sukki,Jheon, Sanghoon,Park, Jae Lippincott Williams Wilkins 2012 JOURNAL OF THORACIC ONCOLOGY Vol.7 No.5

<P>The 15q25 region has been associated with lung-cancer risk and might also be associated with the prognosis of lung cancer. This study was conducted to determine the impact of a functional polymorphism in the CHRNA3 gene on chromosome 15q25 in the survival of patients with early-stage non-small-cell lung cancer (NSCLC).</P>

Flavone Attenuates Vascular Contractions by Inhibiting RhoA/Rho Kinase Pathway

Baek, In-Ji,Jeon, Su-Bun,Song, Min-Ji,Yang, Enyue,Sohn, Uy-Dong,Kim, In-Kyeom The Korean Society of Pharmacology 2009 The Korean Journal of Physiology & Pharmacology Vol.13 No.3

Our previous study demonstrated that flavone inhibits vascular contractions by decreasing the phosphorylation levelof the myosin phosphatase target subunit (MYPT1). In the present study, we hypothesized that flavone attenuates vascular contractions through the inhibition of the RhoA/Rho kinase pathway. Rat aortic rings were denuded of endothelium, mounted in organ baths, and contracted with either 30 nM U46619 (a thromboxane A2 analogue) or 8.0 mM NaF 30 min after pretreatment with either flavone (100 or 300 $({\mu}M$) or vehicle. We determined the phosphorylation level of the myosin light chain ($MLC_{20}$), the myosin phophatase targeting subunit 1 (MYPT1) and the protein kinase C-potentiated inhibitory protein for heterotrimeric myosin light chain phophatase of 17-kDa (CPI17) by means of Western blot analysis. Flavone inhibited, not only vascular contractions induced by these contractors, but also the levels of $MLC_{20}$ phosphorylation. Furthermore, flavone inhibited the activation of RhoA which had been induced by either U46619 or NaF. Incubation with flavone attenuated U46619 or NaF-induced phosphorylation of $MYPT1^{Thr855}$ and $CPI17^{Thr38}$, the downstream effectors of Rho-kinase. In regards to the $Ca^{2+}$-free solution, flavone inhibited the phosphorylation of $MYPT1^{Thr855}$ and $CPI17^{Thr38}$, as well as vascular contractions induced by U 46619. These results indicate that flavone attenuates vascular contractions, at least in part, through the inhibition of the RhoA/Rho-kinase pathway.

Flavone Attenuates Vascular Contractions by Inhibiting RhoA/Rho Kinase Pathway

Inji Baek,Su Bun Jeon,Min-Ji Song,Enyue Yang,Uy Dong Sohn,In Kyeom Kim 대한생리학회-대한약리학회 2009 The Korean Journal of Physiology & Pharmacology Vol.13 No.3

Our previous study demonstrated that flavone inhibits vascular contractions by decreasing the phosphorylation levelof the myosin phosphatase target subunit (MYPT1). In the present study, we hypothesized that flavone attenuates vascular contractions through the inhibition of the RhoA/Rho kinase pathway. Rat aortic rings were denuded of endothelium, mounted in organ baths, and contracted with either 30 nM U46619 (a thromboxane A2 analogue) or 8.0 mM NaF 30 min after pretreatment with either flavone (100 or 300ՌM) or vehicle. We determined the phosphorylation level of the myosin light chain (MLC₂₀), the myosin phophatase targeting subunit 1 (MYPT1) and the protein kinase C-potentiated inhibitory protein for heterotrimeric myosin light chain phophatase of 17-kDa (CPI17) by means of Western blot analysis. Flavone inhibited, not only vascular contractions induced by these contractors, but also the levels of MLC₂₀ phosphorylation. Furthermore, flavone inhibited the activation of RhoA which had been induced by either U46619 or NaF. Incubation with flavone attenuated U46619- or NaF-induced phosphorylation of MYPT1^Thr855 and CPI17^Thr38, the downstream effectors of Rho-kinase. In regards to the Ca²⁺-free solution, flavone inhibited the phosphorylation of MYPT1^Thr855 and CPI17^Thr38, as well as vascular contractions induced by U46619. These results indicate that flavone attenuates vascular contractions, at least in part, through the inhibition of the RhoA/Rho-kinase pathway.

Poster Session:PS 0502 ; Nephrology : Is the New GFR Equation Using Inulin Clearance a More Accurate Method for Asian Patients?

( Beom Seok Kim ),( Yong Kyu Lee ),( Hoon Young Choi ),( Seung Ok Choi ),( Sug Kyun Shin ),( Sung Kyu Ha ),( Kang Wook Lee ),( Yang Wook Kim ),( Yong Lim Kim ),( Yoshinari Yasuda ),( Enyu Imai ),( Mas 대한내과학회 2014 대한내과학회 추계학술발표논문집 Vol.2014 No.1

Background: Recently, a new glomerular fi ltration rate (GFR) equation for the Japanese population was proposed using measured inulin clearance. To expand its applicability to other Asian populations, we performed a comparative study in the Korean population. Methods: Inulin clearance was measured in 166 patients who were selected from seven participating medical centers in Korea. Patient`s sera and urine were collected, and baseline clinical characteristics were measured to provide an estimated GFR (eGFR) by the Japanese GFR equation using inulin clearance (Japanese-GFR equation), the Modifi cation of Diet in Renal Disease (MDRD) study equation, and the Chronic Kidney Disease - Epidemiology Collaboration (CKD-EPI) equation. We compared the equation results to determine which equation best estimated the measured GFR (mGFR). Results: Accuracy (95% CI) within 30% of mGFR by the Japanese-GFR equation, the CKD-EPI equation and the MDRD study equation were 66 (58-72), 51(43-58), and 55 (47-62)%, respectively. Bias (mGFR minus eGFR) were 3.4 22.4, -12.0 22.1, and -9.7 23.8 ml/min/1.73 m2, respectively. The accuracy of the Japanese-GFR equation was signifi cantly better than MDRD study equation in subjects with mGFR <60 ml/min/1.73 m2 and in total subjects. The bias of the Japanese-GFR equation was significantly smaller compared with other two equations in total subjects. The coeffi cient (95% CI) for the Japanese-GFR equation, the CKD-EPI equation, and the MDRD equation in Korean subjects were 1.067 (1.014-1.118), 0.847 (0.808-0.886), and 0.858 (0.816-0.901), respectively, indicating signifi cant overestimation of GFR by MDRD study equation and CKD-EPI equation. There were no adverse events associated with inulin administration during the study. Conclusions: The Japanese-GFR equation has a higher accuracy with less bias than the other equations in estimating GFR in Korean populations.