急峻펄스 電壓을 利用한 强力오존發生裝置開發에 관한 硏究

文在德,吳哲洙,鄭華淑 경북대학교 1985 論文集 Vol.40 No.-

The power efficiency of ozone generation can be substantially improved by modifying a direct discharge wire of a wire-to-cylinder type ozone generator to a helical strip line. and using a fast rising pulse voltage on it. It is raised 1.3 times up of ozone yield compared with that of 60 gO_3/KWh of the industrial silent discharge type ozone generator. Parametric studies also made to get the optimum design of ozone generator tested. The generated ozone concentration had a peak value with proper increasing the crest pulse voltage, the quantity of a pulse forming condenser capacity of the pulse generator, and the interelectrode spacing of self triggered stainless steel spark balls. It is found that the optimum strip line length of the ozone generator tested was about 2m in view point of energy consumption. And, input and terminal impedance also effected greatly to ozone generation, the generated ozone concentration were increased when the input impedance had been 25Ω while the terminal had been opened.

제주도 주요하천의 기저유출량 산정

문덕철,양성기,고기원,박원배 한국환경과학회 2005 한국환경과학회지 Vol.14 No.4

Groundwater in Jeju Island, flowing through main stream, is spring water from underground. To set a fixed quantity of groundwater flowing from surface in a hydrological view, 4 downstream (Woedo stream, Gangjung stream, Yeonwoe stream and Ongpo stream) were selected to calculate the characteristic of baseflow and the base-flow discharge through the data on tachometry. There were 11 to 14 level peak caused by runoff, mostly occurred during monsoon season. Also, duration of runoff was 15 to 25 hours, well reflecting the characteristic of inclined, short stream length in Jeju Island and pervious hydrogeographical feature. In case of Gangjung stream, Yeonwoe stream and Ongpo stream, variation of stream water level by baseflow rose above during summer, which was closely linked to the distribution of seasonal precipitation. From autumn to spring, water level fell below while that of Woedo stream remained the same all year round. Data on the water level observed in Woedo stream and Gangjung stream in every single minutes was applied to weir formula(equation of Oki and Govinda Rao) to calculate baseflow discharge. Also, using the data on current and water level calculated in Ongpo stream and Yeonwoe stream, water level-water flow rating was applied to assess baseflow discharge.

Human Papillomavirus Testing with Hybrid Capture Ⅱ and DNA Chip

이덕철,--,--,-- 대한의생명과학회 2005 Biomedical Science Letters Vol.11 No.1

The detection of high-risk human papilloma virus (HPV) allows us to predict the presence and future development of cervical intraepithelial lesion. In this study, we compared Hybrid Capture II and DNA chip methods for detection of HPV in cervical swab samples. And we evaluated the clinical efficacy and diagnostic performance of HPV DNA chip and Hybrid Capture Ⅱ for detecting HPV in cervical neoplastic lesions. Seventy four patients were classified into three groups according to their histologic diagnosis: Group Ⅰ (nonspecific chronic cervicitis), Group Ⅱ (low-grade squamous intraepithelial lesion (SIL); koilocytosis, and mild dysplasia), and Group Ⅲ (high-grade SIL;, moderate, severe dysplasia and in situ carcinoma). Cytologic diagnosis were based on the Bethesda System. Hybrid Capture Ⅱ and DNA chip methods were performed to detect HPV. In 41 of the 74 cervical samples (55.4%), HPV DNAs were detected by Hybrid Capture Ⅱ. In Group Ⅲ, HPV-positive cases were detected in 15 (20.3%) of 74 patients by Hybrid Capture II. 25 patients with ASCUS cytology were histopathologically examined: 9 cases (36%) were Group Ⅱ. In 18 patients with low-grade SIL cytology, 13 cases (72.2%) were Group Ⅱ and 3 cases (16.7%) were Group Ⅲ. 12 cases (92.3%) were Group Ⅲ of 13 patients with high-grade SIL cytology. The sensitivity of each test was 82% in Hybrid Capture Ⅱ and 53.9% in DNA chip test. And the specificity was 74.3%, 85.7% in Hybrid Capture Ⅱ and DNA chip. In conclusion, Hybrid Capture Ⅱ test is more sensitive than DNA chip in detecting women with cervical neoplastic lesions. Especially, in diagnosing of ASCUS, Hybrid Capture Ⅱ test is more sensitive. Therefore, Hybrid Capture Ⅱ test for cancer-associated HPV DNA is a viable option in the management of women with ASCUS.

가막만 패류양식장의 수질 및 저질특성

최철웅,문성용,윤호섭,라성주,한종석,최상덕,이우범 國立 麗水大學校 環境問題硏究所 2002 環境硏究論文集 Vol.5 No.-

This study was performed to characterize the seasonal fluctuation of water quality and sediment environment from April, 2001 to November, 2001 in Gamak bay. Which was a productive area, but recently its fisheries conditional have been deteriorated because of increasing waste-water from municipal area, industry and autochthonous pollution by farm industry. In the survey of seawater quality, DO and COD concentrations were lowest in bottom layer at summer, but DIN and DIP contents were higher than those in surface layer. Eutrophication indices mean that eutrophication area move from south to north entrance via northern-west inner area with seasonal change from summer to autumn. In the survey of sediment environment, ignition loss(IL), chemical oxygen demand(COD), DIN and Dip were in the range of 3.8~15.7%, 10.3~60.6mg/g-dry, 5.053~1.810mg/ℓ and 0.065~0.324mg/ℓ , respectively. COD in sediment were highest at winter and spring. And, they were higher than sediment standard in the south inner area, which mean heavily polluted conditions with organic constituents.

당뇨병 환자에서 혈장 Thrombin-Antithrombin Ⅲ 및 Plasmin-α_2-Plasmin Inhibitor 복합체의 임상적 의의

김경욱,김은숙,정상수,윤수지,박우일,이준희,남수연,안철우,문병수,김경래,차봉수,송영득,임승길,이현철,허갑범 대한당뇨병학회 2002 Diabetes and Metabolism Journal Vol.25 No.5

연구배경:당뇨병 환자에서 혈액응고 및 섬유소용해 체계의 이상경향이 있어 그 결과로 여러 혈관합병증의 발생위험이 높다는 사실은 널리 알려져 있다. 그 기전은 아직 확실히 밝혀지지 않았으나, 고혈당으로 인한 혈장 단백질들의 비효소성 당화작용이나 산화성 스트레스로 인한 유리 라티칼 작용으로 응고항진이나 섬유소용해 활성의 저하를 유발하는 것으로 생각되고 있다. 최근 응고 및 용해인자와 그 억제자의 복합체들의 증가가 이 상태를 비교적 예민하게 반영한다고 알려져 있다. 방법:본 연구에서는 당뇨병 환자 101명과 정상 대조군 20명에서 혈장내 thrombin­antithrombin complex(TAT)와 plasmin­α₂­plasmin inhibitor complex(PIC)를 측정하여 비교하고, 당뇨병 환자에서 미세혈관 합병증과 대혈관합병증의 유무에 따른 차이와, 이미 혈관 질환의 위험인자로 알려져 있는 인자들간의 상관성을 알아보고자 하였다. 결과:1. 환자의 분포를 살펴보면 혈관합병증이 있는 군은 85명, 혈관합병증이 없는 군은 16명이었고, 평균연령은 각각 57.9±14.1세, 49.9±16.6세로 혈관 합병증이 있는 군에서 더 나이가 많았고, 체질량지수는 23.2±3.4㎏/㎡, 24.1±3.4㎏/㎡로 두 군간 유의한 차이는 없었다. 또 두 군간의 혈압 및 HbA1c, 공복혈당 및 인슐린과 C­peptide, 총 콜레스테롤, 중성지방, HDL­콜레스테롤, Lp⒜는 유의한 차이가 없었고, 미세혈관합병증이 있는 군에서 당뇨병의 유병기간이 길었다. 2. TAT 및 PIC의 농도는 정상 대조군에서는 2.8±1.2 ng/mL, 240.4±69.7 ng/mL이었고, 당뇨병 환자군에서는 9.5±22.6 ng/mL, 472.2±258.7 ng/mL이었다. TAT와 PIC 모두 당뇨병 환자군에서 정상 대조군에 비해 유의하게 증가되어 있었고(p<0.001), TAT/PIC ratio는 두 군간 차이가 없었다. 3. 당뇨병 환자의 혈관합병증에 따른 TAT 및 PIC, fibrinogen 농도는 합병증이 없는 군은 각각 4.1±2.4ng/mL, 362.2±272.0ng/mL, 322.7±102.4mg/mL으로 PIC와 fibrinogen의 증가를 보였으나, 연령을 보정한 후에는 통계학적 유의성은 없었다. 또 대혈관 합병증군에서는 각각 6.0±4.9 ng/mL, 507.4±321.6 ng/mL, 427.1±194.7 mg/dL이었으며 미세·대혈관 합병증군에서는 10.4±6.4 ng/mL, 484.8±269.7 ng/mL, 388.4±132.4 mg/dL으로 TAT의 증가를 보였으나 역시 연령을 보정한 후에는 통계학적 유의성은 없었다. 4. 미세혈관합병증군에서 HbA1c(>8%)가 높은 군의 PIC 농도가 유의하게 높았고(p=0.049), 대혈관합병증군에서 HbA1c(>8%)가 높은 군의 총 콜레스테롤 농도가 유의하게 높았다(p=0.042). 5. 총 당뇨병 환자군에서 PIC는 fibrinogen과 HbA1c와 양의 상관관계를, BMI와 음의 상관관계를 보였으며(r=0.47, 0.31,-0.25), 혈관 합병증이 없는 당뇨병 환자군에서만 TAT는 HbA1c와 양의 상관관계를 보였다(r=0.67). 결론:이상의 결과에서 혈장 TAT 및 PIC 농도는 당뇨병 환자에서 정상 대조군에 비해 의미있게 증가되어 있었고, 당뇨병 환자군에서는 연령의 증가와 유병기간이 혈액응고항진 및 용해의 장애에 큰 역할을 함을 알 수 있었으며, 총 당뇨병 환자군에 PIC와 HbA1c와 양의 상관관계를, BMI와 음의 상관관계를 보였으며 혈관 합병증이 없는 당뇨병 환자군에서만 TAT는 HbA1c와 양의 상관관계를 보였다. 따라서 당뇨병 환자에서 혈액응고 및 용해의 장애가 동반되어 있다고 볼 수 있으며, 혈장 TAT 및 PIC는 혈관합병증으로의 진행을 예측하는 지표로서 유용하리라 생각된다. 또 혈당조절정도와 상관성이 있으므로 혈당조절후에 추적검사를 시행하여 합병증의 예방이 가능한지 추후 연구가 필요하리라 생각된다. Background : Abnormality of coagulation and fibrinolystic system is known as a predisposing factor of vascular complication in diabetes. Although the pathogenesis is not well known, non-enzymatic glycation reaction and the increase in production of free radicals due to an increased oxidative stress may be linked to the hypercoagulibility and hypofibrinolytic activity. As indices of abnormality in coagulation and firinolysis in peripheral blood, plasma thrombin-antithrombin Ⅲ complex (TAT) and plasmin-α_2-plasmin inhibitor complex (PIC) were measured. The purpose of this study was to clarify whether hypercoagulability exists in diabetic patients with or without vascular complication. Methods : In our study, we measured plasma thrombin-antithrombin Ⅲ compelx (TAT) and plasmin-α_2-plasmin inhibit or complex (PIC) in 101 diabetic subjects and 20 controls. Comparing TAT and PIC levels in diabetic microvascular complication group, diabetic macrovascular complication group and controls, we examined correlation between risk factors associated with diabetic vascular complication. Results : 1. The group with diabetic vascular complication was older than group without complication. There was no significant difference in BMI, blood pressure, HbA_ic, blood sugar level, insulin, C-peptide, serum creatinine, total cholesterol, triglyceride, HDL-cholesterol, Lp (a) between two groups. The group with diabetic microvascular complication had longer duration of diabetes. 2. Concentration of TAT and PIC were 2.8±1.2 ng/ mL, 240.4±69.7 ng/ mL in controls and 9.5±22.6 ng/ mL, 472.2±258.7 ng/ mL in diabetic patients, respectively. TAT and PIC were significantly higher in diabetic patients than in control (p<0.001). But TAT/PIC ratio was no significant difference between two groups. 3. In diabetic patients, concentration of TAT and PIC and fibrinogen were respectively 4.1±2.4 ng/ mL, 362.2±272.0 ng/ mL, 322.7±102.4 mg/ dL in group without vascular complication and 5.3±4.1 ng/ mL, 529.5±258.7 ng/ mL, 374.9±106.2 mg/ dL in group with microvascular complication, which group had increase in PIC and Fibrinogen but no significance after correction of age. Concentration of TAT and PIC and Fibrinogen were 60.±4.9 ng/ mL, 507.4±321.6 ng/ mL, 427.1±194.7 mg/ dL in macrovascular complication, and 10.4±6.7 mg/ mL, 484.8±269.7 ng/ mL, 388.4±132.4 mg/ dL in combined vascular complication which group showed increase of TAT but also had no significant increase after correction of age. 4. In diabetic microvascular complication patients, group of high HbA_1c (>8%) (p=0.049) had significant high PIC concentration. In diabetic macrovascular complication patients, group of high HbA_1c (>8%) (p=0.042) had significant high total cholesterol concentration. 5. In all diabetic patients, PIC was positively correlated with fibrinogen and HbA_1c and negatively correlated BMI (r=0.47, 0.31, -0.25). Only in daibetic patients without angiopathy, TAT was positively correlated with HbA_1c (r=0.67). Conclusion : In this study, plasma TAT and PIC concentration significantly increased in diabetic patients compared with controls, and PIC was increased in group with microvascular complication, TAT were increased in group with combined micro macrovascular complication. However, there was no significance relationship existed when correctinf for age. PIC was correlated with HbA_1c. TAT was correlated with HbA_1c only in the group without angiopathy. Abnormality of coagulation and fibrinolysis were combined in diabetes, plasma TAT and PIC can be used as an index of vascular complication. Also we found the correlation with the degree of the blood glucose control. Therefore we need follow up study for the possibility of prevention of vascular complication after controlling the blood glucose to age-matched patients (J Kor Diaabetes Asso 25:354~363, 2001).

Fuzzy Role Extraction using Artificial Neural network

Moon Suk Jang,Duk Chul Chang 대한전자공학회 1994 ICONIP : International Conference On Neural Inform Vol.3 No.2

수모세포종과 원시 신경외배엽 종양 : 임상 및 분자생물학적 분석과 예후에 관한 연구

손문준,전상룡,김저훈,나영신,이정교,권병덕,서재희,이인철 대한신경외과학회 1997 Journal of Korean neurosurgical society Vol.26 No.2

Synthetic Chenodeoxycholic Acid Derivative HS-1200-induced Apoptosis of Human Melanoma Cells

Chul-Jung Baek(백철중),Ji-Hak Min(민지학),Seong-Hyeok Moon(문성혁),In-Ryoung Kim(김인령),Seung-Eun Lee(이승은),Duk-Han Kim(김덕한),Gyoo-Cheon Kim(김규천),Hyun-Ho Kwak(곽현호),Bong-Soo Park(박봉수) 대한체질인류학회 2007 대한체질인류학회지 Vol.20 No.4

담즙산과 합성담즙산유도체가 여러 종류의 암세포에 세포자멸사(apoptosis)를 유도하며, 항암효과가 있다고 알려져 있다. 또한 합성 chenodeoxycholic acid (CDCA) 유도체가 여러 가지 암세포에 유도한 세포자멸사 연구들이 보고되어 왔다. 하지만 아직까지 사람흑색종세포에 합성 CDCA 유도체가 유도한 세포자멸사 연구는 보고되지 않았다. 그래서 본 연구는 합성 CDCA 유도체인 HS-1199와 HS-1200이 사람흑색종세포(G361 세포)에 세포자멸사 효과와 세포자멸사 기작을 밝혀내기 위해서 수행되었다. 합성 CDCA에 처리된 G361 세포의 생존율을 확인하기 위해서 MTT 방법을 사용하였고, 세포자멸사 유도 검증은 DNA 전기영동법과 Hoechst 염색을 이용하였다. 세포자멸사에 관계하는 단백질의 발현 변화와 세포 내에서 이동을 밝혀내기 위해서 Western bot 분석과 면역형광염색법을 수행하였다. 더 나아가서 proteasome 활성도와 사립체막 전위 변화를 측정하였다. 합성 CDCA 유도체로 처리된 G361 세포에서 caspase-3, DFF, PARP의 파괴, caspase-3 (HS-1200 only), PARP, DFF의 분절화, DNA 조각남(HS-1200 only), 핵 응축, proteasome 활성화의 감소, 사립체막전위(MMP)의 감소, 그리고 cytochrome c와 AIF의 사립체에서 세포질로의 유리와 같은 다양한 세포자멸사의 증거를 보였다. 두 개의 합성 CDCA 유도체 중에서 HS-1200이 HS-1199보다 더욱 강한 세포자멸사 효과를 보였다. 본 연구는 CDCA 유도체인 HS-1200이 사람흑색종세포에서 proteasome, 사립체 그리고 caspase 경로을 통해서 세포자멸사를 유도하는 것을 증명하였다. 이러한 결과는 HS-1200이 사람흑색종의 새로운 치료적 전략으로 응용될 수 있다고 생각한다. Bile acids and their synthetic derivatives induced apoptosis in various kinds of cancer cells and anticancer effects. It has been reported that the synthetic chenodeoxycholic acid (CDCA) derivatives showed apoptosis-inducing activity on various cancer cells in vitro. It wasn’t discovered those materials have apoptosis-inducing effects on G361 human melanoma cells. The present study was done to examine the synthetic bile acid derivatives, HS-1199 and HS- 1200, induced apoptosis on G361 cells and such these apoptosis events. The viability of G361 cells was assessed by the MTT assay. Induction of apoptosis was confirmed by DNA electrophoresis and Hoechst staining. Westen blot analysis and immunofluorescent staining were performed to study the alterations in expression level and translocation of apoptosis-related proteins. Proteasome activity and mitochondrial membrane potential (MMP) change were also assayed. Tested G361 cells showed several lines of apoptotic manifestation such as activation of caspase-3, DFF and PARP, DNA degradation (HS-1200 only), nuclear condensation, inhibition of proteasome activity, reduction of mitochondrial membrane potential, and the release of cytochrome c and AIF to cytosol. Between two synthetic derivatives, HS-1200 showed stronger apoptosis-inducing effect than HS-1199 did. Taken collectively, we here demonstrated for the first time that synthetic CDCA dedrivatives induce apoptosis of human melanoma cells through the proteasome, mitochondria and caspase pathway. Therefore our data provide the possibility that HS-1200 could be considered as a novel therapeutic strategy for human melanoma cells from its powerful apoptosis-inducing activity.

Impact of White Matter Changes on Activities of Daily Living in Mild to Moderate Dementia

Moon, So Young,Na, Duk L.,Seo, Sang Won,Lee, Jun-Young,Ku, Bon D.,Kim, Seong Yoon,Park, Kyung Won,Shim, Yong S.,Youn, Young Chul,Chung, Chan-Seung,Cheong, Hae-Kwan,Choi, Seong Hye,Cha, Kyung Ryeol,Kim S. Karger AG 2011 European neurology Vol.65 No.4

<P>The association between white matter changes and activities of daily living (ADL) in a large, well-defined cohort of patients with mild-to-moderate dementia (either Alzheimer’s disease or subcortical vascular dementia) were investigated. A total of 289 patients were divided into three groups (140 mild, 99 moderate, and 50 severe) depending on the degree of white matter changes as indicated on brain magnetic resonance image scans. Further, we analyzed the three groups’ performances on basic and instrumental ADL. The degree of white matter changes was associated with greater age, hypertension, previous history of stroke, higher Hachinski Ischemic Score, worse global cognitive and functional status, and an increased impairment of basic ADL and instrumental ADL. The increased impairment with regard to the severe group’s performance on both the basic and instrumental ADL remained significant after adjustment for age and hypertension. Tasks involving physical activities were most significant. This was the first study investigating the association between white matter changes and ADL in a large, well-defined dementia cohort. The present study suggests that severe white matter changes may be associated with higher impairment on both basic and instrumental ADL.</P><P>Copyright © 2010 S. Karger AG, Basel</P>

CAPD환자에서의 복강도관 생존율과 도관합병증의 관리

문철,황승덕,이진,이희발 대한의학협회 1961 대한의학협회지 Vol.4 No.1