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- 저자 : Donghuo Zhong (검색결과 2 건)
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Donghuo Zhong,Xingnuan Li,Zhongping Wang,Shikai Qi,Mengxi Chen,Chengjin Li,Shan He 한국고분자학회 2024 Macromolecular Research Vol.32 No.4
This study presents the fabrication of polylactic acid microwell patterns using a lithography-based replica molding method for the development of three-dimensional (3D) neural cell-based assays. SH-SY5Y human neuroblastoma cells interfaced with these patterns, and cell growth and morphological characteristics were evaluated with and without an inhibitor of the rho-associated coiled-coil kinase (ROCK) signaling pathway. The results revealed that the culture systems of SH-SY5Y cells on the microwell patterns could be categorized as two-dimensional (2D), 3D, and near-two-dimensional (N2D), according to their location within a dynamic multidimensional culture system. Furthermore, the geometric features of the patterns significantly impact the efficiency of the conversion model, which was characterized by the proliferation and percentage of cells in different culture systems. The rearrangement of the microfilament cytoskeleton and morphological spreading of cells on the patterns were suppressed by the downregulation of the ROCK signaling pathway. The inhibition of the ROCK signaling pathway had a time-dependent effect on the proliferation and migration of cells on the patterned substrate, where topographical cues, cell morphology, and density played pivotal roles. Consequently, the proposed system serves as a practical model for studying the functional behaviors of neural cells by dynamically assembling multidimensional culture systems on the same platform, facilitating the development of cell-based assays.
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A multi-dimensional culture system model for hippocampal NSCs on PLLA microwell pattern
Donghuo Zhong,Wenxu Wang,Sizhe Tang,Tao Ling,Shikai Qi,Xingnuan Li,Zhongping Wang 한국고분자학회 2024 Macromolecular Research Vol.32 No.7
Poly-L-lactic acid (PLLA) microwell patterns were fabricated using a lithography-based replica molding method to develop neural stem cell-based assays. Hippocampal neural stem cells (NSCs) were cultured on microwell patterns to construct a multi-dimensional culture system model in which cells within the microwells were mainly cultured with three-dimensional cellular aggregates (MW-3D cells), and cells on the top surfaces were mainly cultured with two-dimensional single-layer adherent cultures (TS-2D cells). It was found that self-renewal and MW-3D cell-directed migration and transformation regulate the construction of this model. Patterns without channel connections occurred earlier in the construction of this model than those with channel connections, due to the tendency of NSCs to extend to both sides along the direction of the channels. Self-renewal and stemness maintenance of NSCs within the microwells were promoted by the patterns with the channel connections as a suitable microenvironment for a prolonged period of time, and can be used to build quasi-one-dimensional neural networks within the microwells. This will become a practical model for studying the functional behavior of NSCs, with different culture systems dynamically assembled on the same platform for stem cell research and the development of stem cell-based assays.
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