Kainic Acid에 의한 흰쥐 해마 신경원 가지돌기의 독성변화에 대한 광학 및 전자현미경 연구

최진영,김경용,이원복,김동창 대한해부학회 1993 Anatomy & Cell Biology Vol.26 No.4

Combination of Vitamin C and Rutin on Neuropathy and Lung Damage of Diabetes Mellitus Rats

Sohn, Uy-Dong,Je, Hyun-Dong,Shin, Chang-Yell,Park, Sun-Young,Yim, Sung-Hyuk,Kum, Chan,Huh, In-Hoi,Kim, Jin -Hak The Pharmaceutical Society of Korea 2002 Archives of Pharmacal Research Vol.25 No.2

We investigated the role of vitamin C or rutin on neuropathy and lung damage of diabetic mellitus(DM) rats. Norepinephrine content was significantly decreased in sciatic nerves of DM rats compared with non-DM controls but vitamin C had no effect on decreases of norepinephrine. 2,4-dinitrophenylhydrazine (DNPH) incorporation, which is biomarker of protein oxidation, was increased in sciatic nerve of DM rats as compared with normal control. However, vitamin C had no effects on increases of DNPH incorporation . We measured the content of conjugated dienes (CD) as a biomarker of lipid oxidation in sciatic nerve. CD was increased in DM as compared with normal control, Vitamin C or rutin had no effects on increases of CD. However, Rutin plus vitamin C significantly decreased the content of CD as compared with CIM rats. In lung of DM rats, DNPH incorporation or CD was increased as compared with normal control. Vitamin C or Rutin had no effects on increases of CD However, Rutin plus vitamin C significantly decreased the content of DNPH incorporation or CD in lung tissue. Vitamin C caused marked pathological changes such as the increases of parenchyma and the thickening of alveolar septa in the lung of DM. Rutin had protective effects on the pathological changes in the lung of DM rats. In conclusion, Vitamin C had no effects on oxidative parameter, such as DNPH incorporation or CD, and on the decreases of norepinephrine content in DM rats. Vitamin C caused the marked pathological changes in the lung of DM rats but rutin had protective efforts against the pathological changes.

아세틸 콜린 유발 검사시 허혈성 심전도 변화와 흉통의 발생이 내피세포 기능장애에 미치는 영향

최철웅(Cheol Ung Choi),나승운(Seung-Woon Rha),김선원(Sun Won Kim),나진오(Jin Oh Na),임홍의(Hong Euy Lim),김진원(Jin Won Kim),김응주(Eung Ju Kim),한성우(Seong Woo Han),박창규(Chang Gyu Park),서홍석(Hong Seog Seo),오동주(Dong Joo Oh) 대한임상노인의학회 2010 대한임상노인의학회지 Vol.11 No.4

연구배경: 아세틸 콜린 유발 검사시 발생하는 흉통과 허혈성 심전도 변화가 아세틸 콜린에 의해 발생하는 관상동맥의 내피세포 기능에 어떤 영향을 주는지 알아보고자 했다. 방법: 흉통으로 내원하여 관상동맥 조영술과 아세틸 콜린 유발검사를 시행받은 1,085명의 환자를 대상으로 하였다. 아세틸 콜린 유발 검사를 하는 동안 허혈성 심전도 변화 및 흉통의 발생 유무에 따른 관상동맥 내피세포 기능의 특징을 비교하였다. 결과: 539명의 환자에서 아세틸 콜린 검사시 내피세포 기능 장애가 발생 하였다. 허혈성 심전도 변화가 있었던 그룹이 심전도 변화가 없었던 그룹보다 흉통이 더 많았고(78.1% vs. 60.8%, P=0.007), 관상동맥 조영술상 다발성 관상동맥 경련이 빈번하였고(59.4% vs. 40.6%, P=0.004), 관상동맥의 경련 정도도 더 심하였다(64.1% vs. 46.5%, P=0.006). 흉통이 있었던 그룹이 흉통이 없었던 그룹보다 허혈성 심전도 변화의 빈도가 더 높았다(14.7% vs. 7.5%, P=0.007). 또한 흉통이 있었던 그룹이 관상동맥 경련이 다발성으로 발생하는 경우가 많았고 (50.7% vs. 29.5%, P<0.001), 미만성으로 발생하는 경우가 많았고(87.1% vs. 75%, P<0.001), 관상동맥의 경련의 정도도 더 심하였다(51.6% vs. 43.5%, P=0.041). 결론: 아세틸 콜린 유발검사시 발생하는 허혈성 심전도 변화와 흉통은 관상동맥 경련의 정도와 경련이 발생하는 관상동맥 개수와 연관이 있다. Background: ECG changes and chest pain during acetylcholine (Ach) provocation tests may constitute important clinical parameters for Ach-induced endothelial dysfunction. We investigated the association between ischemic ECG changes and chest pain during the Ach-provocation test and angiographic characteristics of Ach-induced endothelial dysfunction. Methods: A total of 1,085 patients with anginal symptoms underwent diagnostic coronary angiography (CAG) and Ach-provocation tests. We compared angiographic characteristics of Ach-induced endothelial dysfunction according to the presence of ECG change and chest pain. Results: A total of 539 patients experienced Ach-induced endothelial dysfunction. Patients who experienced ECG change group suffered more frequent chest pain (78.1% vs. 60.8%, P=0.007), angiographically more frequent multiple coronary artery spasm (59.4% vs. 40.6%, P=0.004), and more severe coronary artery spasm (64.1% vs. 46.5%, P=0.006) than patients without ECG change. However, there was no difference in the length of endothelial dysfunction between the groups. The incidence of ischemic ECG changes in patients with chest pain was higher than in patients without chest pain (14.7% vs. 7.5%, P=0.007). Patients who experienced chest pain more often experienced multiple (50.7% vs. 29.5%, P<0.001), diffuse (87.1% vs. 75%, P<0.001), and severe coronary artery spasm (51.6% vs. 43.5%, P=0.041) than patients without chest pain. Conclusion: Ischemic ECG changes and chest pain during the Ach-provocation test are associated with multi-vessel involvement and severe coronary artery spasm. Chest pain is associated with the length of endothelial dysfunction, but ischemic ECG change is not.

만성 두부외상 환자에서 99mTc - HMPAO Brain SPECT의 임상적 유용성

서정호(Jung Ho Suh),김동익(Dong Ik Kim),정태섭(Tae Sub Chung),이종두(Jong Doo Lee),박창윤(Chang Yoon Park),정진일(Jin Ill Chung),김영수(Young Soo Kim) 대한핵의학회 1992 핵의학 분자영상 Vol.26 No.1

N/A Minima1 deterioration of cerebral perfusion or microanatomical changes were undetectable on conventional Brain CT or MRI. So evaluation of focal functional changes of the brain parenchyme is essential in chronic head injury patients, who did not show focal anatomical changes on these radiological studies. However, the patients who had longstanding neurologic sequelae following head injury, there had been no available imaging modalities for evaluating these patients precisely. Therefore we tried to detect the focal functional changes on the brain parenchyme using Tc-99m-HMPAO Brain SPECT on the patients of chronic head injuries. Twenty three patients who had suffered from headache, memory dysfunction, personality change and insomnia lasting more than six months following head injury were included in our cases, which showed no anatomical abnormalities on Brain CT or MRI. At first they underwent psychological test whether the symptoms were organic or not. Also we were able to evaluate the cerebral perfusion changes with Tc-99m-HMPAO Brain SPECT in 22 patients among the 23, which five patients were focal and 17 patients were nonfocally diffuse perfusion changes. Thus we can predict the perfusion changes such as local vascular deterioration or functional defects using Tc-99m-HMPAO Brain SPECT in the patients who had suffered from post-traumatic sequelae, which changes were undetectable on Brain CT or MRI.

유산양 체세포를 이용한 돼지 난자의 이종간 핵이식 후 배발달에 관한 연구

장석민,나루세겐지,신영민,박창식,진동일 충남대학교 형질전환복제돼지연구센터 2007 논문집 Vol. No.10

This study was conducted to investigate the developmental ability of interspecies cloned embryos after nuclear transfer of goat fetal fibroblast cells into porcien cocytes. Recipient porcine anc goat oocytes were obtained from slaughterhouse and matured III vitro according to established protocols Enucleation was accomplished by aspirating the first polar body and cytoplasm and a single doner cell was individually microinjected into vitelline space of the enucleated oocyte. The reconstructed oocytes were electrically fused WIth O.3M mannitol fusion medium. After electro-fusion, interspecies reconstituted embryos were cultured in PZM-3 for 7 days. In porcine interspecies nuclear transfer With goat fetal fibroblast cells, the cleavage rate of reconstituted ernbryos were 58.9% which was no significant different from that in porcine nuclear transfer embryos (67.4%). However, the developmental rate into blastocyst stage was 5.4% in interspecies nuclear transfer which was significantly lower than that in porcine intraspecies nuclear transfer (13.6%). When the developmental ability of porcine interspecies nuclear transfer with goat cells was compared with goal intraspecies nuclear transfer. the cleavage rate of embryos were 59.2% and the developmental rate into morular and blastocyst stage was 13.6% in intespecies nuclear transfer which were significantly lower than those in intraspecies nuclear transfer embryos. This result indicated that porcine interspecies nuclear transfer with goat fetal fibrobiast cells showed the developmental potential in vitro with lower cleavage and developmental rate compared with intraspecies nuclear transfer.

종이 코팅용 아크릴 에멀젼의 중합물성에 관한 연구(Ⅰ)

장진규,이진우,이동선 동의공업대학 1998 論文集 Vol.24 No.1

Emulsion polymerization of acrylic ester comonomers has been conducted to investigate the effect of anionic-nonionic emulsifier mixture, concentration of initiator and eletrolyte on the size and size distribution of emulsion polymer particles for the purpose of application in the paper coating, It was found that the emulsifier mixture consisting of sodium dodecylbenzene sulfonate and nonylphenoxy po1y(ethyleneoxy)ethanol with high combined HLB value in the range of 14.36∼14.91 gave stable acrylic emulsion with high conversion. The effects of emulsifier, initiator, and electrolyte concentration on the polymer particle size were studied, It was found that acryl emulsion of average particle size (about 0.2 micron with broad size distribution) have desirable moisture vapor transmittance resistance.

지반 개량 범위에 따른 성토지반의 변위거동 분석

장용채,임동진 木浦海洋大學校 2003 論文集 Vol.11 No.-

In case of embankment in soft ground, must know whether base can support as is stable. That improve base using Sand Compaction File Method that is used mainly in soft ground improvement method of construction, recognized using whether when did sphere of soil improving differently, conduct of base by this changes how or 2D-σ for windows that is finite element program to search proper improvement dimension.

피로와 의료이용, 업무상 사고 및 질병결근

장세진,고상백,강동묵,김성아,정진주,이철갑,강명근,현숙정,조정진,차봉석,박종구 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Objectives: This study examined the relationship of fatigue to medical utilization, occupational accident and sickness absence. Subjects and Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project, 2002~2004). A total of 30,146 employees categorized within the Korean Standard Industrial Classification and the Korean Standard Occupational Classification were recruited. A structured-questionnaire was used to assess the participants' sociodemo-graphics, job-related factors, health-related behaviors, personality trait, self=perceived fatigue (MFS) and the frequency of medical utilization, occupational accident and sickness absence. Data on medical utilization and occupational accident were confirmed from company health records. Chi-square test and logistic regression analysis were used to elucidate the relationship of self-perceived fatigue to medical utilization, occupational accident and sickness absence. Results: In logistic regression analyses, self-perceived fatigue was associated with medical utilization (outpatient), occupational accident and sickness absence although the strength of the associations was higher in the blue collar workers except for medical utilization. For medical utilization, the white collar worker's adjusted odds ratios of outpatient utilization for workers scoring in the third quartile and the highest quartile, compared to workers scoring in the lowest quartile were 1.50 (95% CI: 1.20-1.87) and 2.16 (95% CI: 1.69-2.75), respectively. The blue collar workers' adjusted odds ratios of outpatient utilization for workers scoring in the second (OR: 1.23, 95% CI: 1.06-1.43), third (OR: 1.42, 95% CI: 1.22-1.66) and highest quartile (OR:1.83, 95% CI: 1.55-2.17), respectively, compared to workers scoring in the lowest quartile were statistically significant. However, no associations were found between fatigue and inpatient utilization for either the white or blue collar workers. The blue collar workers' adjusted odds ratios of being injured for workers scoring in the second, third and highest quartile were 1.41 (95% CI: 1.00-2.00), 1.57 (95% CI: 1.10-2.22) and 2.41 (95% CI: 1.69-3.44), respectively, compared to those scoring in the lowest quartile, but, those of the white collar workers were not significant. There was more likely to an increased risk of sickness absence in workers scoring in the second, third and highest quartile compared to those scoring in the lowest quartile in both white and blue collar workers, but the magnitudes of risk were higher in the blue collar workers than the white collars workers. Conclusion: This result suggests that fatigue is a determinant predictor of medical utilization, occupational accident and sickness absence, and that the pattern of risks for the three outcomes differs according to the occupational type. Some limitations of this study and considerations for future study were also discussed.

모 중장비 제조 사업장의 유해작업요인 평가에 관한 연구

장성훈,이원진,서창호,김종규,이영신,황천현,서동윤 건국대학교 의과학연구소 1996 건국의과학학술지 Vol.6 No.-

We evaluated the types and the amount of hazards in a heavy equipment factory and a plant factory to improve workers' health and to determine the priority in occupational health programs. We first inspected the working processes to find hazardous agents, measured them in each process environment, and compare the results with their TLVs(Threshold Level Value). This study was carried out from December 26, 1995 to January 30, 1996. The results are as follows: 1. In two factories, we identified the following hazardous agents: noise, dust, heavy metal fume, organic solvents, illumination, gases and ultraviolet light. 2. In the heavy equipment factory, some agents were over their TLVs in the following departments. 1) Preparation Department: dust, Pb, Mn, Fe₂O₃, NO₂ 2) Manufacture Department: noise, dust, Pb, Mn, Fe₂O₃, NO₂ 3) Assembly Department: noise 4) Technique Department: NO₂ 3. In the plant factory, some agents were over their TLVs in the following departments: 1) 1 Bay: illumination, Pb, Mn, Fe₂O₃ 2) 2 Bay: dust, illumination, Cu 3) 3 Bay: illumination 4) 4 Bay: noise, dust, Pb, Mn, Fe₂O₃, NO₂ 5) 5 Bay: noise, dust, Pb, Mn, Fe₂O₃, NO₂ 6) 6 Bay: Pb, Mn, NO₂ We concluded that the hazardous agents such as heavy metal fume, noise and noxious gas were required to be controlled at first.

선천성 고혈압 쥐에서 Candesartan과 Lacidipine의 병용 투여에 의한 혈압하강 상승효과

이동훈,이정진,명창선 충남대학교 약학대학 의약품개발연구소 2010 藥學論文集 Vol.25 No.-

Both candesartan, one of the angiotensin II receptor blocker (ARB), and lacidipine, a Ca2+ channel blocker (CCB), are antihypertensives. This study was designed to examine the drug synergism of ARB with CCB in blood pressure-lowering effect, by combining candesartan with lacidipine. Each telemetered-spontaneous hypertensive rat (SHR) was orally received all seven treatments once with an interval of several days between each injection for washing-out and return to high blood pressure (BP) levels and BP was monitored control (vehicle-treated), candesartan (0.38 mg/kg/day as low-dose; 1.53 mg/kg/day as high-dose), lacidipine (0.1 mg/kg/day as low-dose; 0.38 mg/kg/day as high-dose), and low-dose or high-dose combination candesartan with lacidipine. The results showed that low-dose combination of candesartan and lacidipine exerts significant synergistic BP-lowering effect as compared with high-dose monotherapy of each drug. The present study implies that drug combination of ARB with CCB can offer a clinically important tool for treating hypertension.