Kainic Acid에 의한 흰쥐 해마 신경원 가지돌기의 독성변화에 대한 광학 및 전자현미경 연구

최진영,김경용,이원복,김동창 대한해부학회 1993 Anatomy & Cell Biology Vol.26 No.4

Combination of Vitamin C and Rutin on Neuropathy and Lung Damage of Diabetes Mellitus Rats

Sohn, Uy-Dong,Je, Hyun-Dong,Shin, Chang-Yell,Park, Sun-Young,Yim, Sung-Hyuk,Kum, Chan,Huh, In-Hoi,Kim, Jin -Hak The Pharmaceutical Society of Korea 2002 Archives of Pharmacal Research Vol.25 No.2

We investigated the role of vitamin C or rutin on neuropathy and lung damage of diabetic mellitus(DM) rats. Norepinephrine content was significantly decreased in sciatic nerves of DM rats compared with non-DM controls but vitamin C had no effect on decreases of norepinephrine. 2,4-dinitrophenylhydrazine (DNPH) incorporation, which is biomarker of protein oxidation, was increased in sciatic nerve of DM rats as compared with normal control. However, vitamin C had no effects on increases of DNPH incorporation . We measured the content of conjugated dienes (CD) as a biomarker of lipid oxidation in sciatic nerve. CD was increased in DM as compared with normal control, Vitamin C or rutin had no effects on increases of CD. However, Rutin plus vitamin C significantly decreased the content of CD as compared with CIM rats. In lung of DM rats, DNPH incorporation or CD was increased as compared with normal control. Vitamin C or Rutin had no effects on increases of CD However, Rutin plus vitamin C significantly decreased the content of DNPH incorporation or CD in lung tissue. Vitamin C caused marked pathological changes such as the increases of parenchyma and the thickening of alveolar septa in the lung of DM. Rutin had protective effects on the pathological changes in the lung of DM rats. In conclusion, Vitamin C had no effects on oxidative parameter, such as DNPH incorporation or CD, and on the decreases of norepinephrine content in DM rats. Vitamin C caused the marked pathological changes in the lung of DM rats but rutin had protective efforts against the pathological changes.

아세틸 콜린 유발 검사시 허혈성 심전도 변화와 흉통의 발생이 내피세포 기능장애에 미치는 영향

최철웅(Cheol Ung Choi),나승운(Seung-Woon Rha),김선원(Sun Won Kim),나진오(Jin Oh Na),임홍의(Hong Euy Lim),김진원(Jin Won Kim),김응주(Eung Ju Kim),한성우(Seong Woo Han),박창규(Chang Gyu Park),서홍석(Hong Seog Seo),오동주(Dong Joo Oh) 대한임상노인의학회 2010 대한임상노인의학회지 Vol.11 No.4

연구배경: 아세틸 콜린 유발 검사시 발생하는 흉통과 허혈성 심전도 변화가 아세틸 콜린에 의해 발생하는 관상동맥의 내피세포 기능에 어떤 영향을 주는지 알아보고자 했다. 방법: 흉통으로 내원하여 관상동맥 조영술과 아세틸 콜린 유발검사를 시행받은 1,085명의 환자를 대상으로 하였다. 아세틸 콜린 유발 검사를 하는 동안 허혈성 심전도 변화 및 흉통의 발생 유무에 따른 관상동맥 내피세포 기능의 특징을 비교하였다. 결과: 539명의 환자에서 아세틸 콜린 검사시 내피세포 기능 장애가 발생 하였다. 허혈성 심전도 변화가 있었던 그룹이 심전도 변화가 없었던 그룹보다 흉통이 더 많았고(78.1% vs. 60.8%, P=0.007), 관상동맥 조영술상 다발성 관상동맥 경련이 빈번하였고(59.4% vs. 40.6%, P=0.004), 관상동맥의 경련 정도도 더 심하였다(64.1% vs. 46.5%, P=0.006). 흉통이 있었던 그룹이 흉통이 없었던 그룹보다 허혈성 심전도 변화의 빈도가 더 높았다(14.7% vs. 7.5%, P=0.007). 또한 흉통이 있었던 그룹이 관상동맥 경련이 다발성으로 발생하는 경우가 많았고 (50.7% vs. 29.5%, P<0.001), 미만성으로 발생하는 경우가 많았고(87.1% vs. 75%, P<0.001), 관상동맥의 경련의 정도도 더 심하였다(51.6% vs. 43.5%, P=0.041). 결론: 아세틸 콜린 유발검사시 발생하는 허혈성 심전도 변화와 흉통은 관상동맥 경련의 정도와 경련이 발생하는 관상동맥 개수와 연관이 있다. Background: ECG changes and chest pain during acetylcholine (Ach) provocation tests may constitute important clinical parameters for Ach-induced endothelial dysfunction. We investigated the association between ischemic ECG changes and chest pain during the Ach-provocation test and angiographic characteristics of Ach-induced endothelial dysfunction. Methods: A total of 1,085 patients with anginal symptoms underwent diagnostic coronary angiography (CAG) and Ach-provocation tests. We compared angiographic characteristics of Ach-induced endothelial dysfunction according to the presence of ECG change and chest pain. Results: A total of 539 patients experienced Ach-induced endothelial dysfunction. Patients who experienced ECG change group suffered more frequent chest pain (78.1% vs. 60.8%, P=0.007), angiographically more frequent multiple coronary artery spasm (59.4% vs. 40.6%, P=0.004), and more severe coronary artery spasm (64.1% vs. 46.5%, P=0.006) than patients without ECG change. However, there was no difference in the length of endothelial dysfunction between the groups. The incidence of ischemic ECG changes in patients with chest pain was higher than in patients without chest pain (14.7% vs. 7.5%, P=0.007). Patients who experienced chest pain more often experienced multiple (50.7% vs. 29.5%, P<0.001), diffuse (87.1% vs. 75%, P<0.001), and severe coronary artery spasm (51.6% vs. 43.5%, P=0.041) than patients without chest pain. Conclusion: Ischemic ECG changes and chest pain during the Ach-provocation test are associated with multi-vessel involvement and severe coronary artery spasm. Chest pain is associated with the length of endothelial dysfunction, but ischemic ECG change is not.

만성 두부외상 환자에서 99mTc - HMPAO Brain SPECT의 임상적 유용성

서정호(Jung Ho Suh),김동익(Dong Ik Kim),정태섭(Tae Sub Chung),이종두(Jong Doo Lee),박창윤(Chang Yoon Park),정진일(Jin Ill Chung),김영수(Young Soo Kim) 대한핵의학회 1992 핵의학 분자영상 Vol.26 No.1

N/A Minima1 deterioration of cerebral perfusion or microanatomical changes were undetectable on conventional Brain CT or MRI. So evaluation of focal functional changes of the brain parenchyme is essential in chronic head injury patients, who did not show focal anatomical changes on these radiological studies. However, the patients who had longstanding neurologic sequelae following head injury, there had been no available imaging modalities for evaluating these patients precisely. Therefore we tried to detect the focal functional changes on the brain parenchyme using Tc-99m-HMPAO Brain SPECT on the patients of chronic head injuries. Twenty three patients who had suffered from headache, memory dysfunction, personality change and insomnia lasting more than six months following head injury were included in our cases, which showed no anatomical abnormalities on Brain CT or MRI. At first they underwent psychological test whether the symptoms were organic or not. Also we were able to evaluate the cerebral perfusion changes with Tc-99m-HMPAO Brain SPECT in 22 patients among the 23, which five patients were focal and 17 patients were nonfocally diffuse perfusion changes. Thus we can predict the perfusion changes such as local vascular deterioration or functional defects using Tc-99m-HMPAO Brain SPECT in the patients who had suffered from post-traumatic sequelae, which changes were undetectable on Brain CT or MRI.

지반 개량 범위에 따른 성토지반의 변위거동 분석

장용채,임동진 木浦海洋大學校 2003 論文集 Vol.11 No.-

In case of embankment in soft ground, must know whether base can support as is stable. That improve base using Sand Compaction File Method that is used mainly in soft ground improvement method of construction, recognized using whether when did sphere of soil improving differently, conduct of base by this changes how or 2D-σ for windows that is finite element program to search proper improvement dimension.

유산양 체세포를 이용한 돼지 난자의 이종간 핵이식 후 배발달에 관한 연구

장석민,나루세겐지,신영민,박창식,진동일 충남대학교 형질전환복제돼지연구센터 2007 논문집 Vol. No.10

This study was conducted to investigate the developmental ability of interspecies cloned embryos after nuclear transfer of goat fetal fibroblast cells into porcien cocytes. Recipient porcine anc goat oocytes were obtained from slaughterhouse and matured III vitro according to established protocols Enucleation was accomplished by aspirating the first polar body and cytoplasm and a single doner cell was individually microinjected into vitelline space of the enucleated oocyte. The reconstructed oocytes were electrically fused WIth O.3M mannitol fusion medium. After electro-fusion, interspecies reconstituted embryos were cultured in PZM-3 for 7 days. In porcine interspecies nuclear transfer With goat fetal fibroblast cells, the cleavage rate of reconstituted ernbryos were 58.9% which was no significant different from that in porcine nuclear transfer embryos (67.4%). However, the developmental rate into blastocyst stage was 5.4% in interspecies nuclear transfer which was significantly lower than that in porcine intraspecies nuclear transfer (13.6%). When the developmental ability of porcine interspecies nuclear transfer with goat cells was compared with goal intraspecies nuclear transfer. the cleavage rate of embryos were 59.2% and the developmental rate into morular and blastocyst stage was 13.6% in intespecies nuclear transfer which were significantly lower than those in intraspecies nuclear transfer embryos. This result indicated that porcine interspecies nuclear transfer with goat fetal fibrobiast cells showed the developmental potential in vitro with lower cleavage and developmental rate compared with intraspecies nuclear transfer.

음향방출기법을 이용한 모르터와 콘크리트 보의 균열의 평가와 검출

진치섭,신동익,장종철 부산대학교 생산기술연구소 2000 生産技術硏究所論文集 Vol.59 No.-

내하력 평가를 중심으로 한 종래의 안전성 평가방법은 국부적인 결함의 탐지를 외관조사에 의존하기 때문에 내부균열의 발견 및 발달 양상의 파악에 문제점이 있다. 따라서 종래의 안전진단방식의 단점과 한계성을 보완할 수 있는 합리적인 비파괴검사법의 도입이 필요하다. 고체 내부의 미세균열 발생시 발생하는 탄성파 방출을 이용한 음향방출기법은 내부의 미시적 변형이나 파괴거동 규명에 뛰어난 특성을 가지고 있으며, 광범위한 부분에 대해 외관 조사시 드러나지 않는 재료내부 결함을 검출할 수 있다. 따라서 본 연구에서는 3점 휨 하중을 받는 모르터와 콘크리트 부재의 하중 단계별 미시적 파괴 특성을 음향방출 변수와 비교 · 분석하여, 재료내부의 미세균열이 외형상 거시 균열로 진행되기 전의 균열 전파거동과 파괴징후를 비파괴적으로 알아보았다. Concrete structures generally have clacks, so for the safety and durability of structures, studies to detect cracks using nondestructive tests have been treated in great deal. In order to assure the reliability of concrete structure, microscopic fracture behavior and internal damage progress of concrete under the loading should be fully understood. The purpose of this study predicts location of initial crack and measures direction of crack propagation for on-line monitoring before the crack really grows in structures by using two-dimensional Acoustic Emission(AE) source location based on rectangular method with three-point bending test. This will allow efficient maintenance of concrete structure through monitoring of internal cracking based on AE method

한국인 직무 스트레스 측정도구의 개발 및 표준화

장세진,고상백,강동묵,김성아,강명근,이철갑,정진주,조정진,손미아,채창호,김정원,김정일,김형수,노상철,박재범,우종민,김수영,김정연,하미나,박정선,이경용,김형렬,공정옥,김인아,김정수,박준호,현숙정,손동국 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Background and Purposes: Over the past three decades, numerous studies performed in Korea have reported that job stress is a determinant risk factor for chronic diseases and work disability. Every society has its own culture and occupational climate particular to their organizations, and hence experiences different occupational stress. An occupational stress measurement tool therefore needs to be developed to estimate it objectively. The purpose of this study is to develop and standardize the Korean Occupational Stress Scale (KOSS) which is considered to be unique and specific occupational stressors in Korean employees. Subjects and Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project: 2002-2004). A total of 12,631 employees from a nationwide sample proportional to the Korean Standard Industrial Classification and the Korean Standard Occupational Classification were administered. The KOSS was developed for 2 years (2002-2004). In the first year, we collected 255 items from the most popular job stress measurement tools such as JCQ, ERI, NIOSH and OSI, and 44 items derived from the a qualitative study (depth interview). Forty-three items of KOSS, in the second year, were retained for use in the final version of the KOSS by using Delphi and factor analysis. Items were scored using conventional 1-2-3-4 Likert scores for the response categories. Results: We developed eight subscales by using factor analysis and validation process: physical environment (3 items), job demand (8 items), insufficient job control (5 items), interpersonal conflict (4 items), job insecurity (6 items), organizational system (7 items), lack of reward (6 items), and occupational climate (4 items). Together they explained 50.0% of total variance. Internal consistency alpha scores were ranged from 0.51 to 0.82. Twenty-four items of the short form of the KOSS (KOSS-SF) were also developed to estimate job stress in the work setting. Because the levels of the subscales of occupational stress were gender dependent, gender-specific standard norms for both the 43-item full version and the 24-item short form using a quartile for the subscales of KOSS were presented. Conclusion: The results of this study suggest that KOSS might be an appropriate measurement scale to estimate occupational stress of Korean employees. Further and more detailed study needs to be conducted to improve the validity of this scale.

Changes of Sugar Composition and Related Enzyme Activities during Fruit Development of Asian Pear Cultivars ‘Niitaka’ and ‘Whangkeumbae’

Jin-Ho Choi,Jang-Jeon Choi,Chang-Seok Bang,Jung-Sup Lee,Dong-Woog Choi,Hye-Sun Cho,Jun Cheul Ahn 한국원예학회 2009 Horticulture, Environment, and Biotechnology Vol.50 No.6

We report on the seasonal changes of soluble sugar accumulation and several enzyme activities related to sugar metabolism, in two Asian pear cultivars (Pyrus pyrifolia) cultivars ‘Niitaka’ and ‘Whangkeumbae’. Soluble sugar content increased as the fruit developed and its total contents in matured fruits were 104.6 and 95.1 ㎎ㆍg?¹ fresh weight for ‘Niitaka’ and ‘Whangkeumbae’, respectively. However, the fructose-glucose-sucrose-sorbitol ratio was not significantly changed. Sorbitol-6-phosphate dehydrogenase activity was maintained at high level in leaves and low in fruits throughout the fruit development, although the enzyme activity highly fluctuated in young and mature fruits. NAD-dependent sorbitol dehydrogenase activity increased sigmoidally in both leaves and fruits during fruit development, and correlated with accumulation of total sugar in fruits. Sucrose synthase and sucrose phosphate synthase (SPS) activities remained low in leaves, but higher with two peak fluctuations in fruits. The change of soluble sugar contents positively correlated with SPS activity, based on the seasonal fluctuation of SPS activity and soluble sugar accumulation.

생분해성 Polycaprolactone의 합성

張瑛郁,金容眞,金東國 漢陽大學校 基礎科學硏究所 1995 基礎科學論文集 Vol.14 No.-

다양한 에스터 중합용 금속 촉매중에서 ε- caprolactone을 중합하는데는 tin octoate가 가장 우수한 촉매활성을 나타내었다. 괴상중합에서 촉매농도가 증가하면 분자량이 감소하였고 반응온도가 높아지면 분자량과 수율이 증가하였으며 중합온도 180℃, 촉매 0.036㏖%, 중합시간 50분에서 분자량이 41,000인 polycaprolactone을 얻었다. 용액 중합에서도 유사한 경향을 보였다. Lipase가 용해되어 있는 pH 7.4인 완충용액에서 30일간 침지한 polycaprolactone은 분자량이 높을수록 분해가 느려지는 현상을 파단신율의 측정으로 알 수 있었다. The investigation of effective catalyst system for the polymerization of ε- caprolactone showed that tin octoate, among the various catalysts used for polycondensation, was exhibited as having the high catalyst activity. In bulk polymerization the molecular weight of resulting polycaprolactone was decreased with increasing the concentration of tin octcate, while yields and molecular weight were increased with increasing of reaction temperature. The polymerization of ε- caprolactone was carried out using 0.036㏖% catalyst at 140℃ for 50min to give polycaprolactone with a molecular weight of 41,000. The solution polymerization underwent in the same manner as the bulk polymerization. The prepared polycaprolactone was hydrolysed with lipase in pH. 7.4 buffer solution for 30days and its lose of elongation at breaking point determined revealed that polycaprolactone with high molecular weight was degradated slower than that with low molecular weight.