白朮散과 白扁豆가 白鼠 小腸과 大腸 杯狀細胞內 粘液質에 미치는 影響

金鎭澤,金兌勳,金東煥 동국대학교 한의학연구소 1993 東國韓醫學硏究所論文集 Vol.2 No.2

This study was performed to identify the effect of Backchulsan and Backpeundu, on the mucosubstances of the goblet cells in the small and large intestines of the mouse. After injected castor oil into oral cavity, the experimental animals were administrated with 5.952mg per 30g body weight of Backchulsan, with 0.519mg per 309 body weight of Bckpeundu. The specimens used in this study were obtained from the small and large intestines of the mouse. All specimens were stained with periodic acid-Schiff reaction, alcian blue pH 2.5 and alcian blue pH 1.0 procedures. The results were as follows 1. The most remarkable decrease of neutral and acid mucosubstance was observed in the control group 1 and 6 hours after castor oil administration. 2. Increase of neutral and mucosubstance was observed in Backchulsan administration group 3 hours in comparision with that of control group administrated of castor oil, especially of the upper and lower villi regions. 3. Increase of neutral and acid rnucosubstance was observed in Backpeundu administration group 6 hours in comparision with that of control group administration of castor oil. 4. The most remarkable increase of neutral and acid mucosubstance was observed in Backchulsan administration group after 3 hours in comparision with that of Backpeundu administration group.

Cyclosporin A 로 유도된 생쥐 림프절의 세포성 면역억제에 관한 면역조직화학적 연구 : T 림프구, IL-2 수용기 및 NK세포의 변화를 중심으로 Based on the change of T lymphocytes, Il-2 receptors, and NK cells

김진택,박인식,안상현,최난희,김동환 동국대학교 한의학연구소 1998 東國韓醫學硏究所論文集 Vol.6 No.2

본 실험은 cyclosporin A(CsA)에 의한 시간의 경과에 따른 림프절에서의 세포성 면역억제를 조사하기 위해서 시행된 것으로 BALB/C계 생쥐에 10일동안 CsA(45mg/kg/day) 투여 후 림프절에서의 T 림프구, IL-2 수용기 그리고 자연살해(NK)세포의 분포 변화를 관찰하였다. 대조군의 림프절에서는 L3T4(CD4)에 양성반응을 보이는 도움 T림프구, Ly2(CD8)에 양성반응을 보이는 세포독성 T 림프구 그리고 CD25R에 양성반응을 보이는 IL-2 수용기를 가진 세포는 곁피질(paracortex)과 수질동(medullary sinus)에서 분포하였다. CsA투여 후 처음 3일까지는 이들 양성반웅세포의 분포 변화는 없었으며 양성반응성의 변화도 없었다. 그러나 CsA 투여 7일부터 양성반응 세포수의 감소와 양성반응성의 약화가 관찰되기 시작하였으며 이러한 변화는 시간이 경과하여 14일에 이르렀을 때 가장 큰 감소추세로 나타났다. 한편 NKl.1(CD56)에 양성반응을 보이는 자연살해세포는 피질과 수질에 분포하였으며 CsA 투여 후 시간의 경과에 따라 양성반응 세포수가 감소하였으며, 이러한 감소는 14일에서 가장 큰 것으로 나타났다. 이상의 결과로 미루어보아 CsA 투여는 림프절에서의 IL-2 분비저해를 통해 T 림프구와 NK세포의 활성을 차단하여 선택적이면서 효과적인 세포성 면역억제작용을 하고 있는 것으로 사료된다. Cyclosporin A(CsA) is a selective immunosuppressive agent that has been credited with improved survival of solid organ allografts. Lymph node of BALB/C mouse administered CsA immunohistochemically observed to understand immunosuppressive effects of CsA on T lymphocytes, IL-2 receptors, and natural killer NK cells in lymph node. CsA orally administered daily for 10days at the dose 4smg/kg/day/. The lymph node were obtained at day 3, 7, and 14 after CsA administration and embeded with paraffin, and then stained by following ABC method that used monoclonal antibody including L3T4(CD4), Ly2(CD8), IL-2R(CD25), and NK-1.1(CD56). There were little changes of reactive degree and number of helper T lymphocytes, cytotoxic T lymphocytes, IL.-2 receptors, and NK cells at day 3 after CsA administration, but they began to decrease at day 7. These decrease were greatest at day 14. The helper T lymphocytes, cytotoxic T lymphocytes, IL-2 receptors, and NK cells distributed in paracortex and medullary sinus. These results indicated that the secretion of IL-2 began to decrease at day 7 after CsA administration and subsequently to suppress T lymphocytes and NK cell as components of cell-mediated immunity.

충수주위 농양의 임상적 고찰

김동환,정우식,류성열,장태수 대한대장항문학회 1997 Annals of Coloproctolgy Vol.13 No.2

보중익기탕 합 향사육군자탕이 복수암 유발 생쥐 비장의 세포성 면역활성에 미치는 영향에 관한 면역조직화학적 연구

김호현,김동환 세명대학교 한의학연구소 2001 韓醫學硏究所 論文集 Vol.3 No.-

This study investigated that cellular immunity of spleen is effected by administration of Bojungikgitang and Hyangsayukgunjatang extract (BHE) in Sarcoma 180 cells bearing mice (SBA). The BHE was orally administered daily for 21 days at the dose of 0.743 g/㎏ after Sarcoma 180 cells were intraperitonealiy injected with 1.0×106 cells. The specimen was immunohistochemically stained by Avidin biotin complex methods using monoclonal antibody to L3T4 (CD4), Ly-2 (CD8), IL-2R-α (CD25R-α) and NK-1.1 (CD56) after embedding in paraffin. In BHE treated mice, the number of helper T cells, cytotoxic T cells, and IL-2 receptors are remarkaboy increased in the periarterial lymphatic sheath of white pulp (WP) and penicillary artery and trabecular artery in red pulp (RP). The degree of positive reactions is stronger than SBA. The number of natural killer cells is dramatically increased in marginal zone of WP and penicillary artery of RP and degree of positive reactions is stronger than SBA. Above results show that BHE is effective to increase cellular immunity. It can be considered that the BHE may play a role as efficacious antitumor agent.

連翹敗毒散加味方이 알러지성 접촉피부염에 미치는 影響2 : - 알러지성 접촉피부염 유발로 손상된 생쥐 상피세포 완화를 중심으로 -

김호연,김동환 세명대학교 한의학연구소 2001 韓醫學硏究所 論文集 Vol.3 No.-

After allergic contact dermatitis (ACD) elicitated by Dinitrochlorobenzene treatment, BALB/c female mice administered Yunkyopaedocksangamibang (YPGM) extract are observed to investigate the mitigative effect of YPGM on ACD. The BALB/C mice are sensitized by one application of 25 ㎕ of 5% 2,4-dinitrochlorobenzene (DNCB) onto an abdominal skin and 2 weeks later, the mice were challenged with 4 ㎕ of 2.5% DNCB. The YPGM extract (0.51 g/㎏/day) were daily administered to mice suffered from ACD. At obsevation of epidermal morphology, diminishment of hyperplasia, decrease of damaged epithelial cell in stratum spinosum, and decrease of infiltration of lymphocyto are seen in YPGM treated mice. At obsevation of fine epidermal structures, nuclear shrinkage, cytoplasmic vacuolation, and enlarge of intercellular space are diminished in YPGM treated mice. At observation of TUNEL stain, the evoke of apoptosis is decreased in YPGM treated mice. As results indicated that the YPGM extract administration work on the mitigation of epidermal damages in mice with ACD.

소설의 영원한 내적 형식 : 길 그리고 여행

김동환 한성대학교 인문과학연구원 2010 소통과 인문학 Vol.11 No.-

This article aims to examine the journey form which is important on the development stage of novels. The discussion begins from the question journey form still will have validity in korean modern novels. For the discussion, I divide the journey form into three type - returning to starting point type, opened linear type, closed linear type. The analysis is focused on the texts won the Lee-Sang Literature Awards Grand Prize. The award representatives award systems in korea novels. The conclusions are as follows, 1. In korean modern novels, journey form is still meaningful device. Over Half of the targets contain the form. 2. The space that is essential factor of the form, appears very multiply. After 1990's specific city and place in foreign country is embodied more meaningful device than domestic. 3. In returning to starting point type it tend to be based on the epistemology as the individual’s approach, by comparison in linear type as the social collective’s approach. 4. The novelistic critical mind is more deeply embodied in closed linear type than returning to starting point type.

장기 알콜투여가 생쥐의 간 및 신장, 위장 조직 손상에 미치는 영향

김진택,김동환,안상현 東國大學校韓醫學硏究所 1994 東國韓醫學硏究所論文集 Vol.3 No.-

Alcohol is a major risk factor for several diseases and excessive, long-term alcohol consumption are cause physical alteration-fatty liver, hepatitis, cirrhosis, breaking down, Wernicke-karasakoffs syndrome, weight loss, and poor immunity-in virtually all organ and tissue, This study was observed that liver, kindney, and stomach were altered in mouse by the effect of chronic alcohol administration. The mouse were sacrificed to obtain the tissue after mouse were orally injected with 25% ethanol 18㎖/kg/day for 120days. The tissue were stained by hematoxylin and eosun and then obseved by light microscope. The results of this study were as follows : 1. The congestion was appeared in liver after 120days alcohol administration 2. The destruction of glomerulus were increased and parietal cell of Bowman's capsule were swelled such as cuboidal cell after 12days alcohol administration. The congestion was appeared in alcohol administrated group. 3. The mucosa and gasstric pit were destructed and the ulceration was appeared in stomach after 120days administration. The parietal cells and chief cells were damaged. Above results were shown that the tissue were damaged by chronic alcohol administration.

NOD/SCID 마우스 모델을 이용한 인간 제대혈 혼합이식에서의 생착 양상 분석

정양조,김동욱,조빈,강영주,박보배,김혜정,김태규,오일환 대한조혈모세포이식학회 2002 대한조혈모세포이식학회지 Vol.7 No.2

연구배경: 제대혈은 골수에 비해 많은 장점을 가지고 있음에도, 이식 후 성적에 있어 총세포수에 의해 좌우되며, 한 개의 태반으로부터 얻을 수 있는 총세포수의 한계가 있어 주로 소아에 국한되고 있다. 따라서 제대혈 응용의 확대를 위해 생착량을 늘이기 위한 방법으로 다중공여자에 의한 제대혈 혼합이식을 늘일 수 있는지 연구하기 위하여, 본 연구에서는 주조직적합성 형별로 조합된 제대혈을 NOD/ SCID 생쥐에 이식하는 방법으로, 혼합생착의 가능성을 연구하였다. 방법: 조합된 2개 공여자로부터 얻어진 제대혈들을 단일이식 및 혼합이식하여, 혼합이식된 숙주에 생착된 세포를 PCR-SSOP 방법을 사용하여 공여자 별로 추적하였다. 또한 각 공여자세포의 상대적 생착비율을 대조군인 단일제대혈 이식에서 얻어진 생착량과 비교하는 정량적 비교도 함께 하였다. 결과: 총단핵구를 혼합이식한 실험군에서는 주조직적합성의 일치도에 관계없이 한쪽공여자의 세포가 다른쪽에 비해 우세한 편향생착을 보였다. 그러나 임파구를 비롯한 Lineage 양성세포를 제거한 실험군에서는 주조직적합성의 불일치에도 불구하고 뚜렸한 생착의 공존이 관찰되었다. 결론: 본 연구는 제대혈의 생착량을 증가를 목적으로 총단핵구를 혼합이식할 경우 한쪽공여자의 세포에 의해 편중되며, 이것을 이식 후 생착과정에서의 두 종류의 세포의 경쟁에 의해 초래된다는 것을 보이고 있다. 또한 이러한 경쟁은 제대혈에 포함되어 있던 임파구들에 의한 것이며, 생착공존을 위하여는 공여자간 주조직적합성의 일치도 보다, 임파구의 제거가 더 중요하다는 것을 보여주고 있다. Background: Although umbilical cord blood (UCB) has been an attractive alternatives for bone marrow cells, application is limited to pediatric case due to limitation in total number of nucleated cells, that is a single most important factor for outcomes of UCB transplantation. Therefore, increasing overall engraftment by mixed transplantation of UCB derived from multiple donor should comprise one strategy to circumvent the posed limitation of UCB transplantation. In order to investigate the feasibility of establishing co-engraftment by multi-donor UCB cells, we carried out a xenotransaplantation study using NOD/SCID mice for systemic analysis of results on the input-based control of single unit transplantation. Methods: UCB units with various extent of HLA-matchings were co-transplanted into NOD/SCID mice along with single unit transplantation control. The relative contributions of engraftment by cells from each donor-derived were analyzed by HLA polymorphism using PCR-SSOP. Results: In all HLA-based mixed transplantation of total nucleated UCB cells, engraftment of one donor predominated over the other despite that equivalent amount of engraftments were achieved by single donor transplantation. When lineage depleted UCB units were co-transplanted, significant degree of co-engraftments were observed regardless of HLA disparity. Conclusion: Our result show that one donor cells dominate over the other in mixed UCB transplantation and that it is due to competition between donor cells during post-transplantation process. Our results suggest that immune cells contained in UCB unit mediate such competitioin and that 6 locus HLA matching would not be sufficient to prevent the competition.

Identification and Decryption of Fully phase-encrypted image Using Joint Transform Correlator Structure

Dong-Hoan Seo,Chang-Mok Shin,Kyu-Bo Cho,Jong-Yun Kim,Soo-Joong Kim 대한전자공학회 2004 ITC-CSCC :International Technical Conference on Ci Vol.2004 No.7

Image encryption using phase-based virtual image and interferometer

Dong-Hoan Seo,Chang-Mok Shin,Jong-Yun Kim,Jang-Keun Bae,Jeong-Woo Kim,Soo-Joong Kim 대한전자공학회 2002 ITC-CSCC :International Technical Conference on Ci Vol.2002 No.7