우리나라에서 솔껍질깍지벌레의 분포확산

임유진,이상명,김동수,김준범,최광식,이신혜,박정규,이동운,Lim, Eu-Gene,Lee, Sang-Myeong,Kim, Dong-Soo,Kim, Joon-Bum,Lee, Shin-Hye,Choi, Kwang-Sik,Park, Chung-Gyoo,Lee, Dong-Woon 한국응용곤충학회 2012 한국응용곤충학회지 Vol.51 No.1

솔껍질깍지벌레는 우리나라 곰솔림의 주요한 해충의 하나이다. 본 연구는 우리나라에서 솔껍질깍지벌레의 분포를 알아보기 위하여 7개도 3개 광역시, 91개 시군구, 686 읍면동의 해송림에서 2010년 밀도를 조사하였다. 5개도 2개 광역시에서 솔껍질깍지벌레가 서식하고 있었는데 시군구별로는 조사대상의 64.8%인 59개 시군구에서 서식이 확인되었다. 솔껍질깍지벌레의 분포는 남해안 전역과 동해안의 포항, 서해안의 충남 보령지역까지 분포하였다. 충북과 대전광역시 및 제주도에서는 솔껍질깍지벌레의 분포가 확인되지 않았다. 부산지역은 16곳의 모든 구에서 솔껍질깍지벌레가 분포하였고, 시군 단위에서는 전남 해남군의 솔껍질깍지벌레 평균밀도가 1.713마리/0.785 $cm^2$로 가장 높았으며 읍면동 단위에서는 해남군 송지면이 6.36마리/0.785 $cm^2$로 가장 높았다. 곰솔 가지 내의 솔껍질깍지벌레 밀도와 솔껍질깍지벌레가 서식하고 있는 빈도와는 높은 상관관계가 있었다(상관계수=0.89). The black pine bast scale, $Masucoccus$ $thunbergianae$ (Hemiptera: Margarodidae), is a serious pest of the Japanese black pine, $Pinus$ $thunbergii$, in Korea. The distribution of the black pine bast scale was examined, looking overall at 686 towns (eup), townships (myeon) or neighborhoods (dong). There were Japanese black pine ($Pinus$ $thunbergii$) forests in 91 cities, counties (gun) and borough (gu), in seven provinces and three metropolitan cities during 2010. Black pine bast scale were found in 64.8% of cities or counties or borough (59) in 7 provinces and 3 metropolitan cities, and were distributed in all South Costal regions, Pohang in East Costal region and Boryeong in West Costal region. Chungcheongbukdo, Daejeon and Jeju did not have black pine bast scale. All the gu regions in Busan had black pine bast scale, of which the area with the highest prevalence was Haenam in Jeollanamdo (1.713 crawlers/0.785 $cm^2$). Songji-myeon had the highest occurrence rate (6.36 crawlers/0.785 $cm^2$) from the towns, township and dong. The density of black pine bast scale in twigs was highly correlated with percentage of the sample with scale (Correlation coefficacy=0.89).

와벽 이개도가 레진 인레이의 시멘트 두께 및 접착 강동에 미치는 영향

신동훈,조용범,차윤석 大韓齒科保存學會 1996 Restorative Dentistry & Endodontics Vol.21 No.2

유전자조작 농산물의 개발현황과 그 안전성 평가

신동범 제주대학교 환경연구소 1998 환경연구논문집 : 제주대 Vol.6 No.-

In recent years, the safety of foods and food ingredients from genetically modified plant varieties has been widely discussed. Genetical modification technique to transfer genetic information from one species to another has been possible for about two decades. The ability to transform, or insert new DNA into the plant cell, exists now for most crops, including maize, wheat, soybean, cotton, and rice. Early products of this technology include plants with enhanced insect and disease resistance, and improved herbicide tolerance. Future goals are to increase higher yield, improve nutritional and processing characteristics, and produce specialty oils, carbohydrates, and proteins. Some examples of genetically modified products that have been commercialized are FLAVR SAVR™ tomato, Bollgard^㉿ cotton, Round-Up Ready㉿ soybean, Maximizer™ and NatureGard™ corn, Laurical㉿ Canola oil, and Feedom IF" squash. The international agencies. OECD and WHO, and USDA. FDA and EPA in United States issued a policy statement on foods derived from new plants varieties each other. The document from FDA discussed criteria that should be considered by plant breeders and food manufacturers in assessing the safety of foods derived from new plant varieties before they enter the marketplace. Even though genetically modified plants and their foods are now evaluated safe enough by scientific experiments. the present scientific knowledges won't the truth forever. The ultimate judges of new products of biokhnology will be consumers. They will be more confident in the safety of foods derived from plants deveolped by new technology if scientist and developers share information on safety testmg.

3-deoxyglucosone에 의한 단백질의 modification

신동범,오상환,양융 연세대학교 산업기술연구소 1990 논문집 Vol.22 No.1

pH 7.4, 37℃의 생리적 반응조건에서 HSA, ovalbumine, BSA, lysozyme, RNase A 및 IgG와 3-deoxyglucosone을 반응시키고 단백질의 중합현상과 아미노산 잔기의 손상상태를 연구하였다. 3-deoxyglucosone은 모든 단백질을 중합시킬 수 있으나 특히 lysozyme, RNase A 및 IgG를 현저하게 중합시켰다. 또한 Arginine잔기를 크게 손상시켰다. glucose에 의한 단백질의 중합과정은 glucose가 단백질의 아미노 그룹과의 반응으로 3-deoxyglucosone을 형성시키고, 이것이 단백질의 lysine 잔기와 arginine 잔기를 공격하여 cross link를 형성시키는 것으로 추정되었다. Human serum albumin, bovine serum albumin, ovalbumin, IgG, lysozyme and ribonuclease A were reacted with 3-deoxyglucosone under physiological conditions of 37℃ and pH 7.4, and polimerization of proteins and impairments of amino acid residues were investigated. Proteins tested, especially lysozyme, IgG and ribonuclease A, were polymerized significantly, and lysine residue and arginine residue, espectially arginine residue of proteins were impaired remarkably. Experimental results strongly suggested 3-deoxyglucosone, formed from proteins-glucose reaction system, was the cross-linker responsible for the polymerization of proteins.

미생물 포괄고정화를 위한 고정화재의 비교검토

신동범,윤창훈,강경수 제주대학교 환경연구소 1997 환경연구논문집 : 제주대 Vol.5 No.-

We conducted the experiments to find out the appropriate gelling materials for the treatment of waste water by immobilizing microorganisms inside the gels. The gelling materials known to immobilize microorganisms or cells in them are polyacrylamide, polyvinylalcohol, sodium alginate, κ-carrageenan and chitosan. The gel strengths were determined by the breaking points of the pellet using a rheometer with reaction time. The polyacrylamide gels were retained their solidities and activities excellently compared with the other pellets of polyvinylalcohol, sodium alginate and chitosan with reaction time. It is considered that, in the other hand, the polyacrylamide gels are toxic to the single microorganisms and cells by free radicals during the formation of gels. We used activated sludge for the mmobilization, so it didn't matter the toxicity caused by free radicals. The profiles of waste water treatment by all the immobilized pellets using in this study were showed nearly similar patterns, but gel strength of all the gels except polyacrylamide pellets were getting weak and weak as reaction time goes by. It was concluded that the polyacrylamide gels are excellent for immobilization of activated sludge for waste water treatment, but in the case of immobilization of single microorganisms or cells, natural polysaccharides of sodium alginate, κ -carrageenan or chitosan are thought to be suitable, and it must be developed the techniques for elevating gel strength of nautral polysaccharides.

노화와 관련된 전사인자들

신동범 제주대학교 생명과학연구소 2001 제주생명과학연구 Vol.4 No.-

The biology of aging is still emerging field and, as such, is replete with controversies and contadictions. Although the definition of aging is controversial, in the case of higher animal, it can be defined as a progressive deterioration of biological functions observed only after the organism has attained its maximum reproductive competence. Such age-dependant decline of biological functions involves complex interations among many contributing factors. Among those, oxidative damage caused by oxidative stress is the predominant cause of the age-dependant decline of biological functions. The progressive increase of oxidative stress during aging not only causes oxidative damage to cellular macromolecules, but also modulate the pattern of gene expression through functional alterations of transcription factors. Chronic oxidative stress and inflammatory reaction lead to many age-associated disease such as atherosclerosis and arthritis. Transcription factors that are directly influenced by ROS and proinflammatoy cytokines include nuclear factor kappa B(NF-kB), activator protein 1(AP-1), specificity protein 1(Sp1), peroxisome proliferator-activated receptors(PPARs) and other members of the nuclear receptor superfamily.

생리적조건에서 L-Ascorbic acid의 분해물질

申東範 제주대학교 1993 논문집 Vol.37 No.-

. The degradation products of L-ascorbic acid was examined by incubating it alone and in the presence of α-N-formal-L-Lysine(NFL) at pH 7.4(0.2M phosphate buffer) in the presence of oxygen, metal ion chelator, and in both the presence and absence of sodium cyanoborohydride. Among the large number of degradation products produced, threonic, oxalic, glyceric and glyoxylic acids, as well as threose were all identified by GLC-MS. Decarboxylation is also a feature of the degradation, with approximately 30% of the ascorbic acid undergoing degradation via this pathway. In the presence of NFL and cyanoborohydride ??-N-caboxymethyl-L-lysine(CML) was detected as a reaction product as well. Using GLC-MS as the detection method and ascorbic acid-1-?? as the reactant, only glyoxylic acid and CML were found to contain a ?? Label, indicating that, of the compounds identified above, only these latter two contain C-1 of ascorbic acid.

Glycatoin에 의한 단백질중합체의 가교구조의 탐색

신동범,홍상칠,신완철,윤성식,양융 한국노화학회 1992 한국노화학회지 Vol.2 No.1

Bacillus licheniformis KFB-C14가 생산하는 내열성 Chitinase의 정체 및 특성

홍범식,윤호근,신동훈,조홍연 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.5

Bacillus licheniformis KFB-C14가 생산하는 내열성 chitinase를 30~70% ammonium sulfate fractionation, DEAE-Toyopearl 650M, Butyl-Toyopearl 650M, TSK-Gel Toyopearl HW-55F에 의해 정제도 66배, 수율 21%로 전기영동적으로 균일하게 정제하였다. 정제 단백질은 gel permeation chromatography에 의해 86,000±2,000의 분자량을 나타내었으며, SDS 전기영동에 의해 밝혀진 본 효소의 subunit 구조는 monomer였다. 효소 단백질의 안정성을 검토한 결과 80℃에서 30분 열처리에 의해 56%, 37℃에서 20분간 40% ethanol과 ethyl acetate, 단백질 변성제 등의 처리시에도 50% 이상의 잔존활성을 나타냄으로써 공업적으로 유용성이 높은 안정한 단백질로 판명되었다. 효소반응의 최적 pH와 온도는 pH 6.0과 60℃이었고 Mn^2+ 이온에 의해 효소 활성이 저해되었으나 EDTA, N-ethylmaleimide, p-chloromercuribenzoate 등에 의한 활성감소는 관찰되지 않음으로써 금속효소 또는 thiol계 효소에 속하지 않음을 알 수 있었다. 본 효소는 colloidal chitin, 시판용 chitin에는 반응성이 높았으나 exo형 chitinase의 대표적인 기질인 p-nitrophenyl-2-aectamido-2deoxy-β-glucopyranoside, NN'-diacetylchitobiose에는 전혀 반응성을 보이지 않는 전형적인 endo형의 chitinase였다. 본 효소는 colloidal chitin으로부터 주로 (GlcNAc)_2를, 반응시간 경과에 따라 (GlcNAc)_1과 (GlcNAc)_3을 생성하는 반응성을 보였다. Chitinase (EC 3.2.1.14) from culture fluid of Bacillus licheniformis KFB-C14 was purified 66-folds to homogenity in overall yield of 21% by ammonium sulfate fractionation, DEAE-Toyopearl, Butyl-Toyopearl and TSK-Gel HW-55F column chromatography. The enzyme protein had a molecular weight of about 86,000 and was composed of one subunit. The enzyme was significantly stable not only at high temperature but also on treatment with organic solvents and protein denaturants such as SDS, urea and guanidine·HCl. The optimum temperature and pH for reaction was 60℃ and 6.0,respectively. The enzyme activity was inhibited by only Mn^2+ ion, but not inhibited by EDTA, N-ethylmaleimide and pCMB. The enzyme had high activity with colloidal chitin (V_max: 421) and commercial chitin (V_max :480), but not with typical substrates of exo type chitinase. The thermostable chitinase had an useful reactivity for producing functional chitooligosaccharide, showing the production of (GlcNAc)_1, (GlcNAc)_3, and (GlcNAc)_2 as major product.

Polyphosphate가 연조직 창상 치유에 미치는 영향에 관한 연구

박기범,류동목,이진용,신제원 대한악안면성형재건외과학회 2002 Maxillofacial Plastic Reconstructive Surgery Vol.24 No.3

Polyphosphate has been used as a food additive. It has been approved the use of PolyP to the products as a safe additive. In other study, PolyP seems to has an ability of promoting effect on bone regeneration, possibly by enhancing osteoconductivity of the carrier and/or by increasing osteoinductivity of the damaged bone tissue. So we performed this study in order to evaluate the effect of polyphosphate on the dermal defect healing in critical-sized defect wound. Using welder and blade, a 1㎝ diameter sized burn-defect and rectangular excisional-defect was prepared in the thigh of each Sprague-Dawley rats. Then defect areas were applied topically with distilled water(DW), 0.01% orthoposphate(Pi), or 0.01% polyP75. The application of these materials was continued for seven days. The rats, which had burn wounds, were sacrificed at week 1 and 2. the other goups, which had excisional wounds, were sacrificed at day 3, week 1, 2, and 4. the specimens of the defect were prepared, stained with H&E, and examined under the microscope. Dermal defect healing capacity of polyP75 was also examined by observaing cellular activity of periodontal cells using MTT assay. The results were as follows. PolyP75 at the concentrations of 0.01% increased the MTT activity of periodontal cells by 154.1%. a pathohistologic findings of burn wound with polyP75 showed a faster epidermal healing and more profound maturation in dermis than those of other groups. In excisional wound, compare with other groups, tissue maturity and density of matrix in dermis were much enhanced in the polyP75 groups.