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      • KCI등재

        Identifying genes for resistant starch, slowly digestible starch, and rapidly digestible starch in rice using genome-wide association studies

        Ning Zhang,Maike Wang,Ji Fu,Yi Shen,Yi Ding,Dianxing Wu,Xiaoli Shu,Wenjian Song 한국유전학회 2020 Genes & Genomics Vol.42 No.11

        Background The digestibility of starch is important for the nutritive value of staple food. Although several genes are responsible for resistant starch (RS) and slowly digestible starch (SDS), gaps persist concerning the molecular basis of RS and SDS formation due to the complex genetic mechanisms of starch digestibility. Objectives The objective of this study was to identify new genes for starch digestibility in rice and interprete the genetic mechanisms of RS and SDS by GWAS. Methods Genome-wide association studies were conducted by associating the RS and SDS phenotypes of 104 re-sequenced rice lines to an SNP dataset of 2,288,867 sites using a compressed mixed linear model. Candidate genes were identifed according to the position of the SNPs based on data from the MSU Rice Genome Annotation Project. Results Seven quantitative trait loci (QTLs) were detected to be associated with the RS content, among which the SNP 6 m1765761 was located on Waxy. Starch branching enzymes IIa (BEIIa) close to QTL qRS-I4 was detected and further identifed as a specifc candidate gene for RS in INDICA. Two QTLs were associated with SDS, and the LOC_Os09g09360 encoding lipase was identifed as a causal gene for SDS. Conclusions GWAS is a valid strategy to genetically dissect the formation of starch digestion properties in rice. RS formation in grains is dependent on the rice type; lipid might also contribute to starch digestibility and should be an alternative factor to improve rice starch digestibility.

      • KCI등재

        TRPS1 expression in non-melanocytic cutaneous neoplasms: an immunohistochemical analysis of 200 cases

        Yi A. Liu,Phyu P. Aung,Yunyi Wang,Jing Ning,Priyadharsini Nagarajan,Jonathan L. Curry,Carlos A. Torres-Cabala,Doina Ivan,Victor G. Prieto,Qingqing Ding,조우철 대한병리학회 2024 Journal of Pathology and Translational Medicine Vol.58 No.2

        Background: Although trichorhinophalangeal syndrome type 1 (TRPS1) was initially thought to be highly sensitive and specific for carcinomas and mesenchymal tumors of mammary origin, more recent data suggest its expression is not limited to breast neoplasms but also can be seen in other cutaneous neoplasms, such as extramammary Paget disease and squamous cell carcinoma (SCC) in situ. Methods: Two-hundred cases of non-melanocytic cutaneous neoplasm, including basal cell carcinomas (BCCs) (n = 41), SCCs (n = 35), Merkel cell carcinomas (MCCs) (n = 25), and adnexal neoplasms (n = 99), were tested for TRPS1 expression using a monoclonal anti-TRPS1 rabbit anti-human antibody. Results: TRPS1 expression was present in almost all cases of SCC (94%), with a median H-score of 200, while it was either absent or only focally present in most BCCs (90%), with a median H-score of 5. The difference between BCCs and SCCs in H-score was significant (p < .001). All MCCs (100%) lacked TRPS1 expression. TRPS1 expression was frequently seen in most adnexal neoplasms, benign and malignant, in variable intensity and proportion but was consistently absent in apocrine carcinomas. All endocrine mucin-producing sweat gland carcinomas (EMPSGCs) (100%, 6/6) showed diffuse and strong TRPS1 immunoreactivity, with a median H-score of 300, which was significantly different (p < .001) than that of BCCs. Conclusions: Our study shows that TRPS1 may be an effective discriminatory marker for BCCs and SCCs. It also has a role in distinguishing BCCs from EMPSGCs.

      • KCI등재

        Exploring the Heat Treatment Parameters, Microstructural Evolution, and Mechanical Properties of Ti–6Al–4V Alloy Fabricated by a Selective Laser Melting Process

        Xing Gao,Ning Zhang,Yi Zhang,Mingrui Chen,Yan Ding,Bo Jiang,Yazheng Liu 대한금속·재료학회 2022 METALS AND MATERIALS International Vol.28 No.11

        The effects of heat treatment parameters on the mechanical properties of a selective laser melting Ti–6Al–4V alloy werestudied using an L9 (34) type orthogonal test. Results showed that the solution temperature and aging temperature were themost influential factors relative to the mechanical properties. The optimum heat treatment was then obtained as 920 °C/2 h/water quenching + 550 °C/3 h/air cooling and the corresponding tensile strength was 1045.2 MPa and the elongation was13.6%. Compared to a solution temperature of 850 °C, more fine secondary α phase (αS) and higher geometrically necessarydislocations (GND) density were obtained when solution temperature was 920 °C, which promoted the increased strengthby increasing the interface strengthening effect of αS/β and the dislocation strengthening. However, the microstructure of thecontinuously coarsening αGB resulted in decreased elongation when the solution was at 920 °C. As the aging temperatureincreased from 550 to 650 °C, the GND density was reduced and the microstructures of primary α (αP), αS with variouscrystallographic orientations, lamellar α, and equiaxial αP were coarsened. Therefore, the elongation of the specimen agingat a higher aging temperature increased while the strength decreased.

      • Long short-term memory with wavelet decomposition for wind speed predicting based on SHM data

        Chao-Qun Zeng,Yang Ding,Ning-Yi Liang,Xue-Song Zhang,Jun Wang 국제구조공학회 2025 Smart Structures and Systems, An International Jou Vol.35 No.2

        The wind field environment surrounding long-span bridges is characterized by its complexity and variability, resulting in wind speed exhibiting random, nonlinear, and uncertain behavior. To enhance bridge safety and mitigate the impact of wind speed, it is crucial to establish a reliable wind speed prediction model. In this study, a structural health monitoring (SHM) system was deployed on a long-span bridge to collect extensive wind speed data, which was subsequently denoised using the wavelet decomposition (WD) method. Leveraging the long short-term memory (LSTM) approach, a wind speed prediction model (WD-LSTM) was developed. The study focuses on investigating the effects of three different thresholds (Bayesian threshold, SURE threshold, and Minmax threshold) in the WD method, the number of hidden units (2, 4, 8, 16, 32, 64, 128, 256, and 512) in the WD-LSTM model, and the number of inputs (one-step prediction, five-step prediction, ten-step prediction, and twenty-step prediction) in the WD-LSTM model on the prediction performance of wind speed. Evaluation metrics such as RMSE and R2 are employed for this analysis. Furthermore, the calculation time of the WD-LSTM prediction models with different hidden units and inputs is compared. Finally, an optimal WD-LSTM prediction model is proposed, taking into account both prediction accuracy and calculation time.

      • KCI등재

        Comparison of Apixaban and Low Molecular Weight Heparin in Preventing Deep Venous Thrombosis after Total Knee Arthroplasty in Older Adults

        Ting Guo,Jian-ning Zhao,Hui Jiang,Jia Meng,Yi-cun Wang,Jun Wang,Yang Qiu,Hao Ding 연세대학교의과대학 2019 Yonsei medical journal Vol.60 No.7

        Purpose: To compare the effect of apixaban and low molecular weight heparin (LMWH) in the prevention and treatment of deepvenous thrombosis (DVT) after total knee arthroplasty in older adult patients. Materials and Methods: A total of 220 patients (average age of 67.8±6.4 years) undergoing total knee arthroplasty were randomlyselected as research subjects and were divided into apixaban and LMWH groups (110 in each group). Results: The incidence of DVT was lower in the apixaban group than in the LMWH group (5.5% vs. 20.0%, p=0.001). Activatedpartial thromboplastin times (35.2±3.6 sec vs. 33.7±2.2 sec, p=0.010; 37.8±4.6 sec vs. 34.1±3.2 sec, p<0.001; 39.6±5.1 sec vs. 35.7±3.0sec, p=0.032) and prothrombin times (14.0±1.0 sec vs. 12.8±0.9 sec, p<0.001; 14.5±1.2 sec vs. 13.0±1.1 sec, p<0.001; 15.3±1.4 sec vs. 13.2±1.3 sec, p=0.009) in the apixaban group at 1 week after surgery, 3 weeks after surgery, and the end of treatment were higherthan those in the LMWH group. Platelet and fibrinogen levels in the apixaban group were lower than those of the LMWH group. Also, capillary plasma viscosity and erythrocyte aggregation in the apixaban group at 1 week after surgery, 3 weeks after surgery,and the end of treatment were lower than those in the LMWH group. Conclusion: Apixaban, which elicits fewer adverse reactions and is safer than LMWH, exhibited better effects in the preventionand treatment of DVT after total knee arthroplasty in older adults.

      • SCIESCOPUSKCI등재

        Application of Terahertz Spectroscopy and Imaging in the Diagnosis of Prostate Cancer

        Zhang, Ping,Zhong, Shuncong,Zhang, Junxi,Ding, Jian,Liu, Zhenxiang,Huang, Yi,Zhou, Ning,Nsengiyumva, Walter,Zhang, Tianfu Optical Society of Korea 2020 Current Optics and Photonics Vol.4 No.1

        The feasibility of the application of terahertz electromagnetic waves in the diagnosis of prostate cancer was examined. Four samples of incomplete cancerous prostatic paraffin-embedded tissues were examined using terahertz spectral imaging (TPI) system and the results obtained by comparing the absorption coefficient and refractive index of prostate tumor, normal prostate tissue and smooth muscle from one of the paraffin tissue masses examined were reported. Three hundred and sixty cases of absorption coefficients from one of the paraffin tissues examined were used as raw data to classify these three tissues using the Principal Component Analysis (PCA) and Least Squares Support Vector Machine (LS-SVM). An excellent classification with an accuracy of 92.22% in the prediction set was achieved. Using the distribution information of THz reflection signal intensity from sample surface and absorption coefficient of the sample, an attempt was made to use the TPI system to identify the boundaries of the different tissues involved (prostate tumors, normal and smooth muscles). The location of three identified regions in the terahertz images (frequency domain slice absorption coefficient imaging, 1.2 THz) were compared with those obtained from the histopathologic examination. The tissue tumor region had a distinctively visible color and could well be distinguished from other tissue regions in terahertz images. Results indicate that a THz spectroscopy imaging system can be efficiently used in conjunction with the proposed advanced computer-based mathematical analysis method to identify tumor regions in the paraffin tissue mass of prostate cancer.

      • KCI등재

        Application of Terahertz Spectroscopy and Imaging in the Diagnosis of Prostate Cancer

        Ping Zhang,Shuncong Zhong,Junxi Zhang,Jian Ding,Zhenxiang Liu,Yi Huang,Ning Zhou,Walter Nsengiyumva,Tianfu Zhang 한국광학회 2020 Current Optics and Photonics Vol.4 No.1

        The feasibility of the application of terahertz electromagnetic waves in the diagnosis of prostate cancer was examined. Four samples of incomplete cancerous prostatic paraffin-embedded tissues were examined using terahertz spectral imaging (TPI) system and the results obtained by comparing the absorption coefficient and refractive index of prostate tumor, normal prostate tissue and smooth muscle from one of the paraffin tissue masses examined were reported. Three hundred and sixty cases of absorption coefficients from one of the paraffin tissues examined were used as raw data to classify these three tissues using the Principal Component Analysis (PCA) and Least Squares Support Vector Machine (LS-SVM). An excellent classification with an accuracy of 92.22% in the prediction set was achieved. Using the distribution information of THz reflection signal intensity from sample surface and absorption coefficient of the sample, an attempt was made to use the TPI system to identify the boundaries of the different tissues involved (prostate tumors, normal and smooth muscles). The location of three identified regions in the terahertz images (frequency domain slice absorption coefficient imaging, 1.2 THz) were compared with those obtained from the histopathologic examination. The tissue tumor region had a distinctively visible color and could well be distinguished from other tissue regions in terahertz images. Results indicate that a THz spectroscopy imaging system can be efficiently used in conjunction with the proposed advanced computer-based mathematical analysis method to identify tumor regions in the paraffin tissue mass of prostate cancer.

      • SCIESCOPUSKCI등재

        Functionalization Modification of the Fischer-Tropsch Wax to Improve the Mechanical and Crystallization Properties of the Recycled Polypropylene/Attapulgite Composites

        Tian-Jiao Zhao,Fu-Hua Lin,Shuang-Dan Mao,Ya-Peng Dong,Jia-Le Zhao,Wen-Ju Cui,Shu-Hui Wang,Ding-Yi Ning,Jing-Qiong Lu,Bo Wang 한국고분자학회 2024 폴리머 Vol.48 No.3

        The attapulgite (ATP) with unique one-dimensional rod-like structure was used to improve the mechanical and crystallization properties of the recycled polypropylene (rPP). In order to increase the compatibility of the polar ATP and the non-polar rPP, the maleic anhydride (MAH) was grafted onto the fischer-tropsch wax (FTW) then reacted with ATP to prepare FATP. The tensile, flexural and impact strength of the composites were maximized at the FATP content was 3 wt%, increased by 13.09, 17.56, and 101.92% compared with rPP, and reaching the level of the PP. Due to the improved compatibility, the mechanical properties of the rPP/FATP composites were increased compared with the rPP/ATP composites at the same addition. Moreover, the differential scanning calorimetry (DSC) analysis proved that the addition of the ATP or FATP improved the crystallization properties of the rPP. The crystallization temperature (Tp) of the rPP, rPP/3 wt% ATP, and rPP/3 wt% FATP was 126.31, 127.86, and 129.37 ℃, respectively. The non-isothermal and isothermal crystallization kinetic results showed that the crystallization rate was increased with the addition of the ATP and FATP. Meanwhile, by reason of the synergistic effect of compatibility improvement and internal lubrication, the crystallization rate of the rPP/FATP composites was faster than the rPP/ATP composites. However, the ATP or FATP added in rPP had no effect on the spherulites growth mode of the composites. Above all, the scanning electron micrographs (SEM) results provided intuitive evidence which the compatibility between FATP and rPP was greatly improved.

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