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Nguyen Phuong Thao,Bui Thi Thuy Luyen,Chau Ngoc Diep,Bui Huu Tai,김은지,강희경,이상현,장해동,Nguyen The Cuong,Nguyen Van Thanh,Nguyen Xuan Cuong,Nguyen Hoai Nam,Chau Van Minh,김영호 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.4
This study performed phytochemical and bioactiveassessments of the mangrove Lumnitzera racemosaWilld. leaves. Bioassay-guided fractionation of the methanolicextracts led to the identification of thirty-six compounds(1–36), their structures were elucidated using detailed NMRspectroscopic and MS analysis. The extracts, fractions, andthe isolated compounds were screened for potential antioxidantand cytotoxic activities. Antioxidant assays wereperformed using peroxyl radical-scavenging and reducingassays, whereas cytotoxicity was measured using MTTassays in HL-60 and Hel-299 cell lines. The methanolicextract, CH2Cl2 and n-BuOH fractions (10.0 lg/mL)exhibited potent antioxidant activity, with Trolox equivalent(TE) values of 24.94 ± 0.59, 28.34 ± 0.20, and27.09 ± 0.37 (lM), respectively. In addition, the isolatedcompounds exerted cytotoxic effects in a dose-dependentmanner; compounds 1 and 14 exhibited the most potentcytotoxicity in HL-60 cells, with IC50 values of 0.15 ± 0.29and 0.60 ± 0.16 lM, respectively. To clarify the mechanism(s) behind these cytotoxic effects, we measured thetime-dependent changes in apoptotic markers including thecondensation and fragmentation of nuclear chromatin, andthe downregulation of p-ERK1/2, p-AKT, and c-Myc levels.
Bioconversion of propane to propanol using recombinant Escherichia coli as a biocatalyst
( Nguyen Thi Ngoc Diep ),이은열,이은정,황인엽,( Nguyen Thi Thu ),( Nguyen Duc Anh ) 한국공업화학회 2016 한국공업화학회 연구논문 초록집 Vol.2016 No.0
Methylocella silvestris BL2 is a facultative methanotroph that has a unique ability to grow on methane and to utilize various multi-carbon compounds. M. silvestris BL2 is able to grow on propane by the presence and expression of two gene clusters including a soluble monooxygenase and a putative propane monooxygenase (PrMO) that can convert propane to propanol. In this research, the whole gene cluster of PrMO from M. silvestris BL2 including native promoter and noncoding region was amplified by PCR and successfully cloned into broad host range vector for expression in Escherichia coli. The recombinant E. coli that heterologously expressed PrMO was found to oxidize propane at both terminal and sub-terminal positions as resulting in formation of 1-propanol and 2-propanol, respectively.
( Nguyen Duc Anh ),( Nguyen Thi Thu ),( Nguyen Thi Ngoc Diep ),황인엽,이은열 한국공업화학회 2016 한국공업화학회 연구논문 초록집 Vol.2016 No.0
Natural one-carbon metabolic pathways in native hosts such as methylotrophs have recently been reconstructed in non-native hosts mainly in methanol. Engineering strategy for methanol bioconversion was the implementation of NAD-dependent methanol dehydrogenase (MDH) and co-expressing two key enzymes in ribulose monophosphate cycle (RuMP) were hexulose-6-phosphate synthase (Hps) and 6-phospho-3-hexuloisomerase (Phi). MDH and several Hps-Phi gene clusters as well as artificial fusion forms from different type I methanotrophs were cloned and screened. As a results, in vitro activity of mdh and Hps-Phi were detected. The data provided new opportunities for reconstruction of methanol metabolic pathway from methanotrophs into non-native hosts.