Identification and Characterization of Gonatobotryum apiculatum Causing Leaf Spot and Blight on Sinowilsonia henryi

( Ying Gao ),( Hai Feng Liu ),( Zheng Xing Song ),( Xiao Ying Du ),( Jian Xin Deng ) 한국균학회 2020 Mycobiology Vol.48 No.1

Sinowilsonia henryi is a rare and endangered plant, as well as an endemic species in China. In July 2018, leaf spot and blight disease was observed on S. henryi in Yichang, Hubei, China. A fungus isolated from disease tissues was identified as Gonatobotryum apiculatum based on morphology and sequence analyses of ITS and LSU regions. Phylogenetic analyses indicated that the species belongs to Dothioraceae (Dothideales). Morphologically, the species produced two distinct types of conidia from authentic media, both conidia were described here. Pathogenicity tests showed that the fungus is a pathogen causing leaf spots on S. henryi. This is the first report of leaf spot and blight disease on S. henryi caused by G. apiculatum in China.

Applicability research of round tube CHF mechanistic model in rod bundle channel

Liu, Wei,Peng, Shinian,Shan, Jianqiang,Jiang, Guangming,Liu, Yu,Deng, Jian,Hu, Ying Korean Nuclear Society 2021 Nuclear Engineering and Technology Vol.53 No.2

In view of the complex geometric structure of the rod bundle channel and the limitation of the current CHF visualization experiment technology, it is very difficult to obtain the rod bundle CHF mechanism directly through the phenomenon of the rod bundle CHF visualization experiment. In order to obtain the applicable CHF mechanism assumption for rod bundle channel, firstly, five most representative DNB type round tube CHF mechanistic models are obtained with evaluation and screening. Then these original round tube CHF mechanistic models based on inlet conditions are converted to local conditions and coupled with subchannel analysis code ATHAS. Based on 5 × 5 full-length rod bundle CHF experimental data independently developed by Nuclear Power Institute of China (NPIC), the applicability research of each model for CHF prediction performance in rod bundle channel is carried out, and the commonness and difference of each model are comparatively studied. The CHF mechanism assumption of superheated liquid layer depletion that is most likely to be applicable for the rod bundle channel is selected and two directions that need to be improved are given. This study provides a reference for the development of CHF mechanistic model in rod bundle channel.

Association of Dietary Intake of Folate, Vitamin B₆ and B₁₂ and MTHFR Genotype with Breast Cancer Risk

Liu, Ying,Zhou, Long-Shu,Xu, Xiao-Ming,Deng, Liang-Qing,Xiao, Qian-Kun Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.9

Aim: We aimed to investigate the associations of dietary intake of folate, vitamin $B_6$ and $B_{12}$ and MTHFR genotype with breast cancer in a Chinese population. Methods: A matched case-control study was conducted, and 435 patients with newly diagnosed and histologically confirmed breast cancer and 435 controls were collected. The folate intake, vitamin $B_6$ and vitamin $B_{12}$ were calculated, and MTHFR C665T, C677T and A1298C were analyzed by PCR-RFLP. Results: We found vitamin $B_{12}$ was likely to reduce the risk of breast cancer, and MTHFR 665TT was associated with increased risk of breast cancer. Folate intake, vitamin $B_{12}$ intake and variants of MTHFR C677T and MTHFR A1298C demonstrated no association with risk of breast cancer. However, we found patients with low intake of vitamin $B_6$ and MTHFR 665TT genotype had a higher risk of breast cancer (OR=1.87, 95% CI=1.29-2.77), the association being less pronounced among subjects with a moderate intake of vitamin $B_6$ and MTHFR 665TT genotype (OR=1.58, 95% CI=1.03-2.49, P=0.03). Conclusion: Our study indicated that the MTHFR C665T polymorphism and vitamin $B_6$ are associated with risk of breast cancer, which indicated roles for nutrients in developing breast cancer.

A Filament Level Analysis on 3-D Orthogonal Weave Microgeometry Modeling under Different Yarn Tension

Ying Ma,Yueyan Liu,Congying Deng,Xiang Chen,Yang Zhao,Sheng Lu,Youqi Wang 한국섬유공학회 2020 Fibers and polymers Vol.21 No.10

3-D woven textile is generated through the weaving process. Yarn is a non-continuum material domain, thestructure of which is determined by inter fiber movement. In this study, the micro-geometry of 3-D orthogonal weave isgenerated at filament level predicting through the weaving process implementing the digital element approach (DEA). First,the basic concepts and explicit algorithm of DEA is introduced. The method of calculating adjusted digital fiber materialproperty in terms of discretization resolution is proposed. Second, the unit-cell topology of 3-D orthogonal weave is definedby a position matrix. The calculation of potential energy of the cell is derived. At last, a dynamic weaving process is designedto investigate the effect of yarn tension on fabric micro-geometry and cell energy. 4 unit-cells are generated under 4 sets oftension combination. Results show that as the fabric thickness decrease, the filaments of the weft yarns move towards thecenter. The weft yarns at the top and bottom deform into their final shape first. The applied tension on weaver plays adominant role in determining fabric thickness and convergence speed. By comparing the numerical results with themicroscope pictures taken from the actual specimen, it is concluded that the fabric micro-geometry produced by tensioncombination 4 closely matches the experimental results.

Insulin receptors regulate the fecundity of Nilaparvata lugens (Stål) (Hemiptera: Delphacidae)

Liu Yong-Kang,Luo Yu-Jia,Deng Ying-Mei,Li Yan,Pang Xiao-Qing,Xu Cai-Di,Wang Shi-Gui,Tang Bin 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.4

Two InR (insulin receptor) genes have been identified in the Nilaparvata lugens. In this study, we used RNA interference (RNAi) to investigate the role of InR genes in the fecundity of N. lugens. The expression of NLInR1 and NLInR2 genes was simultaneously silenced with mixture of dsInR1 and dsInR2 (dsInRs) injection. Our results showed that larvae RNAi against both NLInR1 and NLInR2 reduced the number of eggs laid by N. lugens and some eggs as well as ovaries were abnormal. In addition, the relative expression of Vg (vitellogenin) and VgR (vitellogenin receptor) was significantly reduced on the 4th and 6th days after insects treated with larvae RNAi reached the adult stage. We also determined the relative expression levels of insulin/insulin-like signaling (IIS) related genes in RNAi-treated larvae and found that the expression levels of Chico (homologous receptor substrate), Akt (protein kinase B), PI3K (phosphoinositide 3-kinase), and PTEN (phosphatase and tensin homolog) genes decreased whereas FOXO (forkhead box O) and GSK3 (glycogen synthase kinase-3) levels increased on the 4th and 6th days after insects reached the adult stage. These results indicate that silencing of NLInR1 and NLInR2 reduces the fecundity of N. lugens through the IIS pathway.

NVST Data Archiving System Based On FastBit NoSQL Database

Ying Bo Liu,Feng Wang,Kai-fan Ji,Hui Deng,Wei Dai,Bo Liang 한국천문학회 2014 Journal of The Korean Astronomical Society Vol.47 No.3

The New Vacuum Solar Telescope (NVST) is a 1-meter vacuum solar telescope that aims to observe the fine structures of active regions on the Sun. The main tasks of the NVST are high resolution imaging and spectral observations, including the measurements of the solar magnetic field. The NVST has been collecting more than 20 million FITS files since it began routine observations in 2012 and produces a maximum observational records of 120 thousand files in a day. Given the large amount of files, the effective archiving and retrieval of files becomes a critical and urgent problem. In this study, we implement a new data archiving system for the NVST based on the Fastbit Not Only Structured Query Language (NoSQL) database. Comparing to the relational database (i.e., MySQL; My Structured Query Language), the Fastbit database manifests distinctive advantages on indexing and querying performance. In a large scale database of 40 million records, the multi-field combined query response time of Fastbit database is about 15 times faster and fully meets the requirements of the NVST. Our study brings a new idea for massive astronomical data archiving and would contribute to the design of data management systems for other astronomical telescopes.

Functional RsaI/PstI Polymorphism in Cytochrome P450 2E1 Contributes to Bladder Cancer Susceptibility: Evidence from a Meta-analysis

Deng, Xiao-Dong,Gao, Qin,Zhang, Bo,Zhang, Li-Xia,Zhang, Wei,Er, Zhe-Er Mu,Xie, Ying,Ma, Ying,Liu, Yun Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.12

Background: Cytochrome P450 2E1 (CYP2E1) might be involved in the development of bladder cancer. However, previous studies of any association between CYP2E1 RsaI/PstI polymorphism and bladder cancer risk have yielded conflicting results. In this study, we performed a more precise estimation of the relationship by a meta-analysis based on the currently available evidence from the literature. Method: To assess the effect of CYP2E1 RsaI/PstI polymorphism on bladder cancer susceptibility, a meta-analysis of 6 available studies with 1,510 cases and 1,560 controls were performed through Feb 2014. Summary odds ratios (ORs) and corresponding 95% confidence intervals (CIs) were used to estimate the strength of association for CYP2E1 RsaI/PstI polymorphism under different genetic models. Results: When available studies were pooled into the meta-analysis, we found that the C1C2 and C2C2 genotypes of CYP2E1 RsaI/PstI polymorphism significantly decreased bladder cancer risk under different genetic models (heterozygote: OR=0.766, 95%CI=0.613-0.957, $P_{OR}$=0.019; homozygote: OR=0.51, 95%CI=0.303-0.858, $P_{OR}$=0.011; dominant: OR=0.733, 95%CI=0.593-0.905, $P_{OR}$=0.004; recessive: OR=0.565, 95%CI=0.337-0.947, $P_{OR}$=0.030). Subgroup analysis indicated that C2C2 genotype was significantly associated with decreased bladder cancer risk under the homozygote genetic model in Caucasians. There was no evidence of heterogeneity or publication bias. Conclusions: The current meta-analysis suggested that the CYP2E1 RsaI/PstI polymorphism might be associated with bladder cancer susceptibility, especially in Caucasians. Further studies are needed to validate the above conclusion.

Synthesis of metal organic framework (MOF-5) with high selectivity for CO2/N2 separation in flue gas by maximum water concentration approach

Gong-Ying Wang,Zhiyong Deng,Ning Jiang,Shaoying Liu,Cong-Ming Tang 한국화학공학회 2016 Korean Journal of Chemical Engineering Vol.33 No.9

Water plays a crucial role in the synthesis mechanism of metal organic framework-5 (MOF-5). Synthesized MOF-5 with good phase structure and large specific surface area is largely determined by an important synthesis factor: the total water concentration of the initial synthesis solution (Ctw). An understanding of the effects of different and high Ctw on the synthesis of MOF-5 and the investigation of the maximum Ctw suitable for the synthesis of MOF-5 are important to guide the synthesis of MOF-5. Through the research of the maximum Ctw, a favorable synthetic approach was established which could realize the synthesis of MOF-5 with fine performance on CO2 adsorption and separation. The research results show that the maximum Ctw could be as high as 1,440mmol/L, and synthesized MOF- 5 still has a good phase structure and a large specific surface area of 2,136m2/g (BET). Synthesized MOF-5 by the maximum Ctw exhibits a high CO2 adsorption capacity of 2.5mmol/g and a low N2 adsorption capacity of 0.2mmol/g at 298 K and 100 kPa. More importantly, synthesized MOF-5 by the maximum Ctw exhibits a high selectivity for CO2/N2 of 18-22 at 298 K and 20-130 kPa in simulated flue gas.

ZNF552, a novel human KRAB/C2H2 zinc finger protein, inhibits AP-1- and SRE-mediated transcriptional activity

( Yun Deng ),( Bi Sheng Liu ),( Xiong Wei Fan ),( Yue Qun Wang ),( Ming Tang ),( Xiao Yang Mo ),( Yong Qing Li ),( Zao Chu Ying ),( Yong Qi Wan ),( Na Luo ),( Jun Mei Zhou ),( Xiu Shan Wu ),( Wu Zhou 한국생화학분자생물학회 (구 한국생화학회) 2010 BMB Reports Vol.43 No.3

In this study, we report the identification and characterization of a novel C2H2 zinc finger protein, ZNF552, from a human embryonic heart cDNA library. ZNF552 is composed of three exons and two introns and maps to chromosome 19q13.43. The cDNA of ZNF552 is 2.3 kb, encoding 407 amino acids with an amino-terminal KRAB domain and seven carboxyl-terminal C2H2 zinc finger motifs in the nucleus and cytoplasm. Northern blotting analysis indicated that a 2.3 kb transcript specific for ZNF552 was expressed in liver, lung, spleen, testis and kidney, especially with a higher level in the lung and testis in human adult tissues. Reporter gene assays showed that ZNF552 was a transcriptional repressor, and overexpression of ZNF552 in the COS-7 cells inhibited the transcriptional activities of AP-1 and SRE, which could be relieved through RNAi analysis. Deletion studies showed that the KRAB domain of ZNF552 may be involved in this inhibition. [BMB reports 2010; 43(3): 193-198]

Preparation and Characterization of PTFE/PI Nanofiber Composite Assembled Sponges

Huizhong Liu,Dawei Li,Ying Shen,Bingyao Deng 한국섬유공학회 2021 Fibers and polymers Vol.22 No.3

Due to the ultralow density and high specific area, nanofibrous sponges show great potential in the field offiltration and separation. To prepare nanofibrous sponges for high temperature smoke filtration, polytetrafluoroethylene(PTFE) was chosen due to its corrosion resistance and high temperature resistance. PTFE nanofiber prepared viaelectrospinning shows irregular concave-convex pores in the fiber body, which can improve the specific surface area andfurther enhance filtration performance. However, sponges made of PTFE nanofiber suffered from severe shrinkage, whichlimited the development in sponge products. In this study, polyimide (PI) nanofiber converted from polyamic acids (PAAs)nanofiber was introduced into the PTFE sponges to reinforce the dimensional stability. The parameters that influenced themorphology, porosity, shrinkage ratio, and thermodynamic properties of the composite sponges were also investigated. Moreover, the filtration performance of the composite sponges was tested. Results indicated that PTFE/PI compositenanofiber sponges possessed high porosity (94.34-98.12 %), excellent thermal stability (above 550 oC), and decent filtrationefficiency (the maximum filtration efficiency reached 99.97 % to PM 2.0) were prepared, which demonstrated the potential inthe field of high-temperature filtration.