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Cho, Daeho,Kang, Jae Seung,Park, Jong Hoon,Kim, Young-In,Hahm, Eunsil,Lee, Junechul,Yang, Yoolhee,Jeon, Junho,Song, HyunKeun,Park, Hyunjeong,Kim, Taesung,Pang, Saic,Kim, Chul-Woo,Hwang, Young Il,Lee, 전남대학교 약품개발연구소 2002 약품개발연구지 Vol.11 No.-
Based on our recent observation that enhanced IL-18 expression positively correlates with malignant skin tumors, such as SCC and melanoma, we examined the possible role of UVB, known to be associated with skin cancer development, in the enhancement of IL-18 production using primary human epidermal keratinocytes and human cell line HaCaT. After cells were exposed to UVB irradiation in vitro, IL-18 production was examined by Northern blot analysis and ELISA, and it was found that IL-18 production is enhanced by UVB irradiation in a dose- and time-dependent manner. In addition, we confirmed that it is functionally active form of IL-18 using the inhibitor of caspase-1. The effect of UVB irradiation was blocked by antioxidant, N-acetyl-ι-cysteine (NAC), which suggested the involvement of reactive oxygen intermediates (ROI) in the signal transduction of UVB irradiation-enhanced IL-18 synthesis. We also found that UVB irradiation increased AP-1 binding activity by using EMSA with AP-1-specific oligonucleotide. Furthermore, inhibitors of UVB-induced AP-1 activity, such as PD98059, blocked enhanced IL-18 production, indicating that AP-1 activation is required for UVB-induced IL-18 production. Taken together, our results suggest that UVB irradiation-enhanced IL-18 production is selectively mediated through the generation of ROI and the activation of AP-1.
Endogenous interleukin-l8 modulates immune escape of murine melanoma cells
Cho, Daeho,Kim, Youngin,Kang, Jaeseung,Hahm, Eunsil,Lee, Wangjae,Song, Hyunkeun,Pyun, Kwangho,Choi, Inpyo 이화여자대학교 세포신호전달연구센터 2001 고사리 세포신호전달 심포지움 Vol. No.3
It has been known that melanoma cells can suppress the immune system by the Fas ligand. The present study investigated whether interleukin(IL)-18, which can enhance Fas ligand expression, is produced by B16F10 melanoma cells and is involved in immune escape of tumor cells. Immunohistology, RT-PCR, intracellular FACS analysis, and immunoblotting demonstrated that melanoma cells express IL-18. In addition to IL-18, the IL-18 receptor was also detected in B16F10 melanoma cells, suggesting a role of this cytokine in regulating the functions of B16F10 melanoma cells. The functional effect of IL-18 on B16F10 melanoma cells was shown by reduction of Fas ligand expression in cells transfected with IL-18 antisense cDNA. In addition, the same treatments decreased intracellular reactive oxygen intermediate levels in B16F10 melanoma cells, indicating that IL-18 regulates reactive oxygen intermediate production. Furthermore, transfection of IL-18 antisense cDNA into melanoma cells increased the susceptibility of tumor cells to natural killer cells in vitro. When IL-18 antisense transfectants were implanted into syngeneic mice, severe reduction of tumor cell growth was observed. Taken together, these results demonstrate that IL-18 has a critical role as a survival factor for B16F10 melanoma cells.
Lee, Byeong C.;Jung, Mi Y.;Cho, Daeho;Sullivan, InSug O;Cohen, Edward P.;Kim, Tae Sung Sookmyung Women's University Research Institute of 2011 여성과 건강 Vol.6 No.2
This study describes the immunotherapeutic properties of vaccines that encode tumor associated calcium signal transducer 1 (Trop 1), a newly identified breast cancer antigen, in mice with breast cancer. Previously we found that Trop 1 was over expressed in cellular breast cancer vaccines that were highly enriched for cells that induced therapeutic CTL mediated immune responses in mice with breast cancer, as compared with non enriched vaccines. In this study, to determine if the expression of Trop 1 by cells in the enriched vaccine was responsible for its therapeutic benefits, an expression plasmid that specified the Trop 1 gene was transfected into the LM fibroblast cells, which was then used as a vaccine. To augment their immunogenic properties, the fibroblasts were genetically modified before Trop 1 DNA transfer to secrete IL 2 and to express allogeneic MHC class 1 H 2K^b determinants. Mice with established breast cancer treated solely by immunization with fibroblasts modified to express Trop 1 developed CD8^+ cell mediated immunity to the breast cancer cells. The immunity was sufficient to prolong the survival of mice with established breast cancer. In some instances, the immunity was sufficient to result in rejection of the tumor; the mice remained tumor free more than 60 days.
LEE, BYUNG CHEOL;KIM, MYUN SOO;CHO, DAEHO;CHOI, SANG HO;KIM, TAE SUNG Sookmyung Women's University Research Institute of 2011 여성과 건강 Vol.6 No.2
In a previous study, we reported that a gene muta¬tion of repeat in toxin E (RtxE), a transporter of cytotoxic factors, resulted in a significant impairment of epithelial cell cytotoxicity in Vibrio vulnificus, and that the expression of the rtxE gene was induced by the exposure to the host cells. In this study, we evaluated and compared the effects of co¬culture supernatants from V. vulnificus-infectcd INT-407 cells and either the V. vulnificus wild-type or rtxE mutant on the production of interleukin (IL)-8, a pro-inflammatory cyto¬kine, as well as its underlying mechanisms in human intestinal epithelial cells. INT-407 cells were co-cultured with the wild-type V. vulnificus or the rtxE mutant strain to obtain the conditioned supernatants. IL-8 production and nuclear factor (NF)-_(k)B activation from the INT-407 cells treated with each supernatant, were investigated. The co-culture supernatants from the rtxE mutant V. vulnificus-infected INT-407 cells significantly induced lower levels of IL-8 production and promoter activation, NF-_(k)B DNA binding activity, and NF-_(k)B minimal promoter activation in human intestinal epithelial cells, than those from the wild-type V. vulnificus-'infecicd INT-407 cells. Importantly, the reduced IL-8 production and NF-_(k)B activity of the V. vulnificus rtxE mutant, were restored by co-culture supernatants from the rtxE-complemented V. vulnificus. On the whole, these results show that the rtxE gene of V. vulnificus performs a critical role in the secretion of factors from bacteria and host cells, which are involved in IL-8 production via the NF-_(k)B activation pathway in host cells.
Sung, Daeho,Jung, Chulho,Cho, Byeong-Gwan,Jo, Wonhyuk,Han, Hee-Sung,Lee, Ki-Suk,Bhat, Vinayak,Farmer, Barry,De Long, L. E.,Lee, Ki Bong,Keavney, D. J.,Lee, Dong Ryeol,Song, Changyong The Royal Society of Chemistry 2018 Nanoscale Vol.10 No.27
<P>Unraveling nanoscale spin structures has long been an important activity addressing various scientific interests, that are also readily adaptable to technological applications. This has invigorated the development of versatile nanoprobes suitable for imaging specimens under native conditions. Here we have demonstrated the resonant coherent diffraction of an artificial quasicrystal magnet with circularly polarized X-rays. The nanoscale magnetic structure was revealed from X-ray speckle patterns by comparing with micromagnetic simulations, as a step toward understanding the intricate relationship between the chemical and spin structures in an aperiodic quasicrystal lattice. Femtosecond X-ray pulses from free electron lasers are expected to immediately extend the current work to nanoscale structure investigations of ultrafast spin dynamics, surpassing the present spatio-temporal resolution.</P>
워게임에서 환경과 상황을 고려하여 가상군의 행위를 결정하기 위한 온라인 자동계획기법
최대회(Daehoe Choi),조준호(Junho Cho),김익현(Ikhyun Kim),박정찬(Jungchan Park),정성훈(Sung Hoon Jung) 제어로봇시스템학회 2011 제어로봇시스템학회 합동학술대회 논문집 Vol.1 No.1
본 논문에서는 워게임에서 환경과 상황을 고려하여 가상군의 행위를 결정하기 위한 온라인 자동계획 기법을 소개한다. 기존에 개발된 대부분의 워게임에서는 가상군의 행위를 결정하는데 외적인 환경과 상황을 고려하지 않고 단순한 규칙이나 유한상태기계와 같이 결정적인 방법을 사용한다. 이러한 방법은 가상군이 외부 환경의 변화나 상황변화에 대하여 능동적으로 인식하여 대처하지 못하므로 사용 용도에 한계가 있다. 본 논문에서는 가상군이 행위를 결정하는데 있어서 외부환경과 상황을 감안하여 행위를 계획하도록 함으로서 보다 자율적이고 지능적인 가상군을 구현할 수 있게 한다. 자율적이고 지능적인 가상군이 구현된 워게임은 구동 시 적은 운영위원으로 워게임을 구동할 수 있으며 또한 쉽게 실제 훈련과 연동할 수 있기 때문에 많은 장점을 가진다. 본 논문에서는 이러한 자동계획 기법의 기능을 위주로 소개하며 일부의 기능이 구현된 프로토타입 시스템의 결과를 설명한다.
Colletotrichum spp. Agents of Anthracnose on Blueberry Leaves in Gangwon Province, Korea
Byung-Ju Cho,Hyo-Won Choi,DaeHo Kim,JongKyu Lee 강원대학교 산림과학연구소 2021 Journal of Forest Science Vol.37 No.2
Blueberry, which produces phenolic compounds, is one of the most popular fruits in Korea. During a survey on blueberry diseases, 16 Colletotrichum isolates were obtained from blueberry leaves in Chuncheon and Gosung, Kangwon province, Korea. Using morphological and molecular analyses, the isolates were identified as Colletotrichum aenigma, C. fioriniae, C. fructicola, C. gloeosporioides, and C. nymphaeae. C. gloeosporioides was the most frequently isolated (11 isolates), and only one or two isolates of the other species were found. After inoculation with all isolates, those leaves and fruits with wounds easily developed anthracnose; whereas, fruits without wounds became infected but leaves without wounds were infected by only two of C. gloeosporioides. Typically, around seven fungicidal agents are used to control anthracnose on blueberries in Korea. Fluzinam and prochloraz manganese complex strongly (over than 80%) inhibited the growth of all Colletotrichum species, while dithianon and mancozeb only weakly (about 80% or less) inhibited their growth.