보성산 유기농 녹차의 품질에 따른 카테킨 함량과 항산화능 비교 분석

박경련(Kyung Ryun Park),이상길(Sang Gil Lee),남태규(Tae Gyu Nam),김영준(Young Jun Kim),김영록(Young-Rok Kim),김대옥(Dae-Ok Kim) 한국식품과학회 2009 한국식품과학회지 Vol.41 No.1

유기농 녹차의 등급별 총페놀 함량과 항산화능은 각 추출 용매에 따른 수율을 확인하였을 때 물 추출에 비하여 수용성 유기용매의 추출 수율이 유의적으로 높았다. 유기용매를 이용한 추출수율은 물을 이용할 때 보다 총페놀 함량은 1.5에서 3.2배, 항산화능은 1.8에서 3.8배 정도 높게 나타났다. HPLC 분석을 통하여 총페놀 함량과 항산화능의 상관관계를 비교 시 총페놀 함량이 증가함에 따라 항산화능이 1차 선형관계로 증가하는 경향을 보였다. HPLC분석을 통하여 유기농 녹차에 함유되어 있는 다양한 카테킨의 함량이 채엽시기에 따라 변화가 나타났다. 유기농 녹차의 카테킨 성분 중 가장 많이 존재하는 EGCG가 건조중량당 5.8-7.7%의 함량을 보였고, caffeine은 1.7-2.9%의 함량을 보였다. 유기농 녹차의 카테킨 함량은 일반적으로 채엽 시기가 늦어짐에 따라서 점차 감소하였다. 유기농 녹차의 등급에 따른 항산화능은 최상급인 우전에서 가장 높았으며 최하등급인 엽차에서 가장 낮게 나타났다. HPLC 분석에 의한 유기농 녹차의 등급에 따른 총 카테킨 함량(㎎/g)은 우전(155.4), 세작(147.7), 중작(143.2), 엽차(135.1), 대작(130.5)의 순서로 감소하였다. 이를 통해 녹차의 채엽 시기가 유기농 녹차의 카테킨 성분 및 항산화능에도 영향을 미치는 것을 확인하였다. 전반적으로 상급의 유기농 녹차 일수록 총페놀 및 카테킨 함량이 많으며 또한 더 높은 항산화능을 가지는 것으로 나타났다. The objective of this study was to evaluate the effect of various solvents on extraction of bioactive phenolics and to analyze the antioxidant capacity and contents of individual catechins in various grades of green teas organically grown in Boseong, Korea. The organic green teas, based on their harvest seasons, were categorized into five grades such as Woo-Jeon, Se-Jak, Jung-Jak, Dae-Jak, and coarse tea. Solvents used to extract phenolics from these teas included water at 23℃ and 70℃ as well as 80% (v/v) aqueous methanol and ethanol. In general, aqueous organic solvents of methanol and ethanol led to higher extraction yields of phenolics than water at 23℃ and 70℃. Total phenolics and antioxidant capacity of the teas extracted with the aqueous organic solvents were approximately 1.5 to 3.2 and 1.8 to 3.8 times higher than those with water at 23℃ and 70℃, respectively. Coarse tea, the lowest grade of green tea, showed approximately 30-60% lower total phenolics and antioxidant capacity compared with the higher grade ones. Reversed-phase HPLC analysis was performed quantitatively to identify individual catechins, gallic acid, and caffeine in teas extracted with 80% (v/v) aqueous methanol. Based on their dry weights, the organic green teas contained about 1.7 to 2.9% of caffeine. Content (㎎/g dry weight) of tea catechins decreased in the following order: Woo-Jeon (155.4) > Se-Jak (147.7) > Jung-Jak (143.2) > coarse tea (135.1) > Dae-Jak (130.5). (-)-Epigall℃atechin gallate was the most abundant among the catechins analyzed. The highest grade of green tea, Woo-Jeon, had the highest amount of (-)-epigall℃atechin gallate at 77.4 ㎎/g dry weight. Overall, the higher grade of organic green teas tended to have the higher level of antioxidant capacity and catechins.

Conjugated Linoleic Acid (CLA) Production in the Rumen -Roles of Butyrivibrio fibrisolvens A38

Kim, Dae-Ok,Kim, Tae-Wan,Heo, Ho-Jin,Imm, Jee-Young,Hwang, Han-Joon,Oh, Sejong,Kim, Young-Jun Korean Society for Food Science of Animal Resource 2004 한국축산식품학회지 Vol.24 No.3

Conjugated linoleic acid (CLA) is currently under intensive investigation due to its health benefits. A great deal of interest has been paid to the possible health-promoting roles of CLA, but there are not many studies available on the mechanism of CLA production by ruminal microorganisms. CLA is produced as an intermediate of the characteristic biohydrogenation process of linoleic acid(LA) in the rumen and its production has direct relationship to numerous environmental factors including particle association, substrate concentration, forage-to-grain ratio, pH, ionopore, bacterial cell density, etc. Some of these factors were known to affect hydrogenating activities of Butyrivibrio fibrisolvens A38 which is an active rumen bacterium in CLA production. Dairy cow is a main source of CLA, and its level could be increased by dietary manipulation changing the physiological environment of rumen bacteria such as B. fibrisolvens A38. Therefore, the effects of various factors on. ruminal biohydrogenation should be carefully considered to optimize not only CLA production, but also other fatty acid metabolism, both of which are directly affecting nutritional quality and functionality of dairy products. In this review, the relationship between various environmental factors and ruminal CLA production is discussed focusing on the CLA production of B. fibrisolvens A38.

Proangiogenic TIE2+/CD31+ macrophages are the predominant population of tumor-associated macrophages infiltrating metastatic lymph nodes

Kim, Ok-Hee,Kang, Gun-Hyung,Noh, Hyungjoon,Cha, Ji-Young,Lee, Ho-Jae,Yoon, Jeong-Hwan,Mamura, Mizuko,Nam, Jeong-Seok,Lee, Dae Ho,Kim, Young A.,Park, Young Joo,Kim, Hyeonjin,Oh, Byung-Chul Springer-Verlag 2013 Molecules and cells Vol.36 No.5

<P>Tumor-associated macrophages (TAMs) accumulate in various cancers and promote tumor angiogenesis and metastasis, and thus may be ideal targets for the clinical diagnosis of tumor metastasis with high specificity. However, there are few specific markers to distinguish between TAMs and normal or inflammatory macrophages. Here, we show that TAMs localize in green fluorescent protein-labeled tumors of metastatic lymph nodes (MLNs) from B16F1 melanoma cells but not in necrotic tumor regions, suggesting that TAMs may promote the growth of tumor cells and the progression of tumor metastasis. Furthermore, we isolated pure populations of TAMs from MLNs and characterized their gene expression signatures compared to peritoneal macrophages (PMs), and found that TAMs significantly overexpress immunosuppressive cytokines such as IL-4, IL-10, and TGF-β as well as proangiogenic factors such as VEGF, TIE2, and CD31. Notably, immunological analysis revealed that TIE2(+)/CD31(+) macrophages constitute the predominant population of TAMs that infiltrate MLNs, distinct from tissue or inflammatory macrophages. Importantly, these TIE2(+)/CD31(+) macrophages also heavily infiltrated MLNs from human breast cancer biopsies but not reactive hyperplastic LNs. Thus, TIE2(+)/ CD31(+) macrophages may be a unique histopathological biomarker for detecting metastasis in clinical diagnosis, and a novel and promising target for TAM-specific cancer therapy.</P>

Parthenolide suppresses tumor growth in a xenograft model of colorectal cancer cells by inducing mitochondrial dysfunction and apoptosis

KIM, SE-LIM,TRANG, KIEU THE THU,KIM, SEONG HUN,KIM, IN HEE,LEE, SEUNG OK,LEE, SOO TEIK,KIM, DAE GHON,KIM, SANG-WOOK Spandidos Publications 2012 International journal of oncology Vol.41 No.4

<P>Parthenolide (PT), a principal active component in medicinal plants, has been used conventionally to treat migraine and inflammation. This component has recently been reported to induce apoptosis in cancer cells, through mitochondrial dysfunction. In the present study, we investigated PT-mediated cell death signaling pathway by focusing on the involvement of Bcl-2 family members in human colorectal cancer cells. We also investigated the inhibitory effect of PT on tumor growth in xenografts. Using the human colorectal cancer cell lines HT-29, SW620 and LS174T, we demonstrated that treatment of these cancer cells with PT induces apoptosis using MTT, Annexin V assay and Hoechst 33258 staining. Apoptosis through the mitochondrial pathway was confirmed by detecting regulation of Bcl-2 family members, cytochrome c release and caspase activation. Moreover, intraperitoneal injection of PT showed significant inhibition of tumor growth, angiogenesis in the xenograft model. These results demonstrate that PT exhibits anti-cancer activity in human colorectal cancer in vitro and in vivo. These findings may also provide a novel approach for the treatment of colorectal cancer.</P>

Production of Multiple Gene Transgenic Pig for Xenotransplantation of Pancreatic Islet to Treat Type I Diabetes Mellitus in Humans

Ok Jae Koo,Jung Taek Kang,Dae Kee Kwon,Sol Ji Park,Su Jin Kim,Mohammad Atikuzzaman,Ma. Ninia Gomez,Goo Jang,Jong Ik Hwang,Jae seok Yang,Curie Ahn,Byeong Chun Lee 대한수의학회 2009 대한수의학회 학술대회발표집 Vol.2009 No.-

Direct Observation of Molybdenum Disulfide, MoS₂, Domains by Using a Liquid Crystalline Texture Method

Kim, Dae Woo,Ok, Jong Min,Jung, Woo-Bin,Kim, Jong-Seon,Kim, Seon Joon,Choi, Hyung Ouk,Kim, Yun Ho,Jung, Hee-Tae American Chemical Society 2015 NANO LETTERS Vol.15 No.1

<P>Because the properties of molybdenum disulfide (MoS<SUB>2</SUB>) are strongly influenced by the sizes and boundaries of its domains, the direct visualization of large-area MoS<SUB>2</SUB> domains is one of the most important challenges in MoS<SUB>2</SUB> research. In the current study, we developed a simple and rapid method to observe and determine the boundaries of MoS<SUB>2</SUB> domains. The technique, which depends on observations of nematic liquid crystal textures on the MoS<SUB>2</SUB> surface, does not damage the sample and is not limited by domain size. Thus, this approach should significantly aid not only efforts aimed at gaining an understanding of the relationships between grain boundaries and properties of MoS<SUB>2</SUB> but also those focusing on how domain sizes are controlled during large-area synthesis</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/nalefd/2015/nalefd.2015.15.issue-1/nl5034528/production/images/medium/nl-2014-034528_0005.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/nl5034528'>ACS Electronic Supporting Info</A></P>

Puffing, a novel coffee bean processing technique for the enhancement of extract yield and antioxidant capacity

Kim, Wooki,Kim, Sang-Youn,Kim, Dae-Ok,Kim, Byung-Yong,Baik, Moo-Yeol Elsevier 2018 Food chemistry Vol.240 No.-

<P><B>Abstract</B></P> <P>Puffing of coffee beans, which induces heat- and pressure-derived physicochemical changes, was applied as an alternative to roasting. Roasted or puffed coffee beans with equivalent lightness values were compared. The moisture content was higher while the crude fat and protein compositions were lower in puffed beans than in roasted beans. The pH was lower and the acid content was higher in puffed beans than in roasted beans. The roasted beans exhibited greater specific volumes, while the puffed beans displayed greater extraction yields. The trigonelline and total phenolic contents were greater in puffed beans than in roasted beans resulting in an enhanced antioxidant capacity. Sensory evaluation of roasted and puffed coffee bean brews revealed that puffing did not affect the flavor or overall acceptance. The current study provides evidence that puffing is an alternative to roasting coffee beans with various benefits.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Puffing is a novel alternative of roasting in coffee processing. </LI> <LI> Puffing increased extraction yield and antioxidant capacity compared to roasting. </LI> <LI> Puffing did not increase specific volume but increased extraction yield. </LI> <LI> Chlorogenic acid and trigonelline contents significantly reduced with roasting. </LI> </UL> </P>

Dietary intake of folate and alcohol, MTHFR C677T polymorphism, and colorectal cancer risk in Korea

Kim, Jeongseon,Cho, Young Ae,Kim, Dong-Hyun,Lee, Bong-Hwa,Hwang, Dae-Yong,Jeong, Jinyoung,Lee, Hun-Jae,Matsuo, Keitaro,Tajima, Kazuo,Ahn, Yoon-Ok Oxford University Press 2012 The American journal of clinical nutrition Vol.95 No.2

<P>The incidence of colorectal cancer (CRC) is increasing sharply in Korea, and evidence has suggested the role of dietary methyl supply and related polymorphisms on colorectal carcinogenesis.</P>

Characterization of the Asiatic Acid Glucosyltransferase, UGT73AH1, Involved in Asiaticoside Biosynthesis in <i>Centella asiatica</i> (L.) Urban

Kim, Ok Tae,Jin, Mei Lan,Lee, Dae Young,Jetter, Reinhard MDPI 2017 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.18 No.12

<P><I>Centella asiatica</I> (L.) Urban contains two ursane-type triterpene saponins, asiaticoside and madecassoside, as major secondary metabolites. In order to select candidate genes encoding UDP-glucosyltransferases (UGTs) involved in asiaticoside biosynthesis, we performed transcriptomic analysis of leaves elicited by methyl jasmonate (MeJA). Among the unigenes, 120 isotigs and 13 singletons of unique sequences were annotated as UGTs, including 37 putative full-length cDNAs, and 15 of the putative <I>UGT</I> genes were named according to the UGT committee nomenclature protocols. One of them, UGT73AH1, was characterized by heterologous expression in <I>Escherichia coli</I> BL21 (DE3) cells. After induction with IPTG, a total protein extract was assayed with UDP-glucose and asiatic acid. UPLC-QTOF/MS analysis showed that UGT73AH1 catalyzes the glycosylation of asiatic acid to its monoglucoside. It remains unclear whether glycosylation occurs on the triterpene C-2α, C-3β, C-23, or C-28 position. However, it is very likely that UGT73AH1 glucosylates the C-28 position, because only C-28 bears a glucose moiety in the final pathway product of asiatic acid, while C-2α, C-3β, and C-23 remain un-conjugated.</P>

ETRI’s GLORY Project : Resource Management System Using IPMI and Power Management

Dae Won Kim,Sun Wook Kim,Won Ok Kwon,Seung Woon Kim 대한전자공학회 2009 ITC-CSCC :International Technical Conference on Ci Vol.2009 No.7

This paper introduces Resource management system using IPMI (Intelligent Platform Management Interface) and power management in ETRI’s GLORY (Global Resource Management System for Future Internet Service) project. In this project, we implemented server health system and power management system for 512 node cluster system. Server health system uses IPMI OOB(out-of-band) management interface and power management uses dynamic power management based on some polices. For high expendability and portability, we import the proxy master and it has charge of 256 nodes for management. We can save the power up to 20% per node