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Overexpression of Zoysia ZjCIGR1 gene confers cold stress resistance to zoysiagrass
Yang‑Ji Kim,Dae‑Hwa Yang,Mi‑Young Park,Hyeon‑Jin Sun,Pill‑Soon Song,Hong‑Gyu Kang,Seok‑Cheol Suh,Yong‑Eok Lee,이효연 한국식물생명공학회 2020 Plant biotechnology reports Vol.14 No.1
Zoysia japonica Steud. is a warm-season lawn grass popular in Korea and elsewhere. They are cultivated in many places such as river banks, roadside, and play grounds. However, there still is a disadvantage of frequent mowing, and the grass grows poorly under the chilly conditions. To develop a grass variety that circumvents these drawbacks, we cloned the chitininducible gibberellins-responsive1 gene (CIGR1) from zoysiagrass. The full length of the ZjCIGR1 (Zj; Zoysia japonica Steud.) gene was obtained by 5′/3′ RACE PCR and the phylogenetic tree showed that it belonged to the CIGR1-subgroup in the PAT1-group of GRAS protein family. Expression of the ZjCIGR1 in wild-type plants was confirmed in roots, meristems, leaves, and flowers, especially high in the flowers. The transgenic zoysiagrass was confirmed by PCR using gene-specific primers, phosphinothricin-acetyl-transferase (PAT) strip test, and Southern blot analysis. ZjCIGR1-overexpressing plants acquired tolerance to cold stress displaying morphological phenotypes characteristic of stress resistance. In addition, in the transformants, expression of the ZjCIGR1 as well as cold-regulated (COR) gene was increased compared to the wild-type plants under cold stress condition. These results suggest that ZjCIGR1 gene is an important candidate for regulating cold stress resistance.
유세포 측정을 통한 CD34^(+)세포의 정량방법 비교
양형모,서영훈,엄현석,민창기,김희제,정대철,이종욱,진종율,한치화,김춘추,김학주 대한조혈모세포이식학회 1998 대한조혈모세포이식학회지 Vol.3 No.2
본 연구소에서는 현재 CD34^(+) 세포의 정량에 사용되고 있는 4가지 서로 다른 방법을 가지고 골수에 적용하여 그 결과를 비교하였다. 각각의 방법들이 편리한 점과 불리한 점들을 가지고 있었지만, 통계적으로는 유의한 차이를 보이지 않았다. 1) CD34-PE/CD45-FITC 방법13(ISHAGE: International Society of Hematotherapy and Graft Engineering), 2) CD34-PE/CD14-FITC 방법, 3) CD34-PE/CD14-FITC/CD45-PerCP 방법, 4) DNA dye/CD34-PE/CD45-PerCP4(Becton Dickinson) 등을 비교하였으며 그 결과 CD34^(+) 세포의 농도는 다음과 같았다. 1) 0.473±0.345, (%Mean±SD, N=70) 2) 0.663±0.519, (N=20), 3) 0.543±0.381 (N=46), 4) 0.624±0.341, (N=32). 시료로는 여의도 성모병원 카톨릭 조혈모세포이식 센터에서 채혈한 골수를 이용하였다. Four different methods for flow cytometric quantitation of CD34+ cell were compared using bone marrow samples (n=168). Following combination of antibodies were used for the analysis : A) CD45-FITC/CD34-PE (n=70), B) CD14-FITC/CD34-PE (n=20), C) CD14-FITC /CD34-PE /CD45-PerCP(n=46), D) DNA dye/CD34-PE/CD45-PerCP (n=32). Each method showed no significant differences among them (P=0.126, ANOVA test, SPSS Version 7.5). When we compare these methods using same samples (n=12), the P value became 0.832. The mean % of CD34+ cells calculated in this experiment were A) 0.474%, B) 0.663%, C) 0.548%, D) 0.624% respectively. In our laboratory, we decided to use CD45-FITC/CD34-PE methods as a routine protocol.
남기택,오상연,조현무,이국경,강진석,제정환,최미나,한상욱,김대용,장동덕,양기화,안병우 식품의약품안전청 2001 식품의약품안전청 연보 Vol.5 No.-
feficotorfer fyf☞ri(Hp)가 위암파 관련이 있다는 역학적인 증거는 많이 있지만 이에 대한 정확한 기전에 대해선 밝혀져 있지 않고 있으며 실험동물 모델도 적절하지 못한 것으로 알려져있다. 본 실험에서는 위암의 원인으로 알려져 있는 f, fyforf'(Hp)를 이용하여 마우스에서 위암 모델을 확립하고 만성염증과정 중에 생성되는 리0와 COX-2 등의 발현이 위암발생에 미치는 명향을 통하여 예방과 치료를 위한 점근을 시도하고자 하였다. 마우스를 7군으로 나누어서 1, 2, 3, 4군의 등물은 MNU를 증류수에 200ppm 농도로 음수병득 이용하띤 10주간 격주로 투여하였으며 MHU 음술 투여 1주 휴씩 후 배양한 f. fyrofi 를 약 109cru/rfll 로 맞춰 한 마리당 0.1ml 씩 이틀 간격으로 세 번에 걸쳐 하룻방 금식시킨 1, 2, 3, 5, 6, 7군기 마우스의 위장에 투입하떴다. 균 투입을 마친 후 다응 날부터 2군쏙 6군은 iNOS 억제제인 aminoguanidine(AG)을 음수병으로 툰여하였으며 3군과 7군은 COX-2 척제제인 nimesulide(NSD)를 투여하였다. 위의 종양발생양상을 샅최보면 bfNU와 Hp만을 투여한 1 관 ; (hfNU +Hp), 2군 : iNO을 inhibitor 투여군(MNU+HP+AG'1. 3군 ;CO딘-2 Inilibitor 투여군(MNU누Hp누 NSD), 4군 ,MNlf 단독투여군, 5군 ;Hp 단독투여춘, 6군 ; 러p 단독에 AG투여군, 7군 , Hp 단독에 NSD투여군의 종양발샐을은 각각 쁜.Bff(l1/16), 70.6%f12/ti), 했.9ff(7/18), 10%(1/10), Off(O/IS)0%(O/S), 0%(O/5)의 발쟁율을 보여 iNOS 억제제인 AC은 좁양발생을 억계하지 못하였으며, COX-2억제제인 NSD 는 종양발생을 유의적으로 감소시켰다. 콩양발생개수에서는 2.62±0.36, 1.41츠0.14, 0.44 르0.12, 0.10±0.10을 보여 AC와 NSD에서 유의성 있게 발생개수를 줄였다. Hp 단독투여에 의해즌는 종양발생이 나타나지 않았으며 HP+AG, Hp+ IfSD 추여군에콕 시험증료 시점에 약물에 의해 Hp의 제균효과가 있는지의 여부를 확인끓기 위하여 PCR을 이용하여 확인한 결과 모두 양성인 것으로 나타나 Hp의 제균효과에 의한 촐양발생 억제가 일어나지는 않았다. 위의 결과로 볼 때 Hp는 위암발생을 촉진하는 것으로 나타났고 딘p 감염시 매개되는 염증인자를 억제하였을 때 종양발생을 억제하는 것으로 위암 발생에서 염증매개인자는 종양을 촉진하는 것으로 나타났으며 it,705 억제제쓱 COX-』 억제제의 위알 예밭효과fl는 효과적일 것으로 사료된다 In spite of a large volume of epidemiological evidence indicating significant relationship between H. pylori infection and gastric adenocarcinoma, a doubt still exists on an elevated risk of stomach cancer by H. pylori infection due to lack of direct evidence of their exact mechanistic link. It is, therefore, essential to have an appropriate animal model for detailed analysis of the role of H. pylori played in gastric carcinogenesis. There is a wealth of evidence to support that over production of inducible nitric oxide synthase(iNOS) and cyclooxygenase-2(COX-2) is involved in the pathogensis of various cancer in both rodents and humans. The aim of this study was to establish a mouse model for H. pylori-associated gastric carcinogenesis and to identify the role of inducible nitric oxide synthase(iNOS) and cyclooxygenase-2(COX-2) played during the gastric carcinogenesis in mice. Eighty-three specific pathogen free, six-week-old male C57BL/6 mice were randomly divided into seven groups. Animals of the group 1, 2, 3, 4 were given MNU in their drinking water at the concentration of 200 p.p.m. for total five cycles of one-week regimen with one-week pause. After completion of MNU administration, they were given autoclaved distilled water for one weeks, and groups 1, 2, 3, 5, 6, 7 were inoculated with H. pylori. After completion of H. pylori. inoculation, groups 2 and 6 were given aminoguanidine in their drinking water at concentration of 1000p.p.m. and animals of group 3 and 7 were given the diet containing 200 ppm nimesulide at 12 weeks of age. All animals were killed at 50 weeks of age. The incidences of the glandular stomach tumors in the group 1, 2, 3 and 4 were 87.5%(14/16), 76.4%(13/17), 44.4(8/18), 10.0%(1/10), respectively and the tumor incidence of group 3(MNU→Hp+nimesulide) was significantly lower than those of group 1(MNU→Hp) at the value of P<0.01. The average numbers of tumors of group 2(MNU→Hp+AG : 1.41±0.24) and group 3(MNU→Hp+nimesulide : 0.44±0.12) were significantly lower than those of group 1(MNU→Hp : 2.62±0.36) at the value of P<0.05. Therefore, overproduced iNOS and COX-2 plays an important role in mice gastric carcinogenesis. We concluded iNOS and COX-2 inhibitor have good effects on gastric carcinogenesis.
저온에서 녹기연장의 한국잔디 ‘한라그린 7’ 품종 개발
양대화(Dae-Hwa Yang),선현진(Hyeon-Jin Sun),정옥철(Ok-Cheol Jeong),강홍규(Hong-Gyu Kang),송인자(In-Ja Song),진일두(Il-Doo Jin),강시용(Si-Yong Kang),이효연(Hyo-Yeon Lee) 한국육종학회 2021 한국육종학회지 Vol.53 No.4
The ‘Halla Green 7’ cultivar is a hybrid of Zoysia matrella and Zoysia japonica. The inflorescences of Z. matrella lines werecollected from Jeju Island near the border between Z. matrella and Z. japonica wild plants, respectively. After their harvest, the seeds weresown and various interspecific lines were obtained. In 2021, a line with greening period extending phenotype during the low temperaturesof winter was registered under the cultivar name of ‘Halla Green 7’ (Registration No. 245). The main morphological characteristics of the‘Halla Green 7’ variety are as follows: genetically short length of plant (12.2±0.58 cm), very wide angle of leaf (68.6°), short length ofleaf blade (8.38±0.37 cm), medium-leaf (3.29±0.09 mm), and very low leaf sheath (2.18±0.19 cm). The short length of leaf sheath makesit easy to manage the turf grass with low height. A very wide leaf angle has a high photosynthetic efficiency. The ‘Halla Green 7’ cultivaris morphologically classified as Z. matrella × Z. japonica hybrid line by medium-width of leaf blade and the presence of trichomes on theleaf surface. The colors of the leaf blade, leaf sheath, and stolon are green, light green, and light purple, respectively. During the low temperatureperiod of winter, chlorophyll content of the leaves of ‘Halla Green 7’ shows a 10.1-fold from that of the control Z. japonia ‘Yaji’ and about4.2-fold from that of the control Z. matrella Zm-6. This greening period extending phenotype is expected to contribute to the industrializationof zoysiagrass (Registration No. 245).
강대화(Dae-Hwa Kang),홍준희(Jun-Hee Hong),곽양양(Yang-Yang Guo),이혁원(Hyok-Won Lee) 한국생산제조학회 2013 한국생산제조학회지 Vol.22 No.4
In this study, we developed a counting method for non-contact ID cards using an optical fiber displacement sensor instead of the traditionally used friction counting method. The proposed method has the advantage of high speed and automated measurement. For counting non-contact ID cards, an H-type optical fiber sensor, jig part, and counting program are developed separately to build the system and adjust it. Through the experimental test results, it was confirmed that counting is possible with one type of international ID card and one type of financial security cardbased on ISO7810. Furthermore, by applying the proposed method to 100 ID cards 100 times repeatedly, it was confirmed that it has high accuracy and an error ratio of 0%. We experimentally demonstrated that the proposed counting method for non-contact ID cards using an optical fiber displacement sensor can perform measurements with high accuracy and high speed.
양대화(Dae-Hwa Yang),정옥철(Ok-Cheol Jeong),선현진(Hyeon-Jin Sun),강홍규(Hong-Gyu Kang),진일두(Il-Doo Jin),이효연(Hyo-Yeon Lee) 한국육종학회 2021 한국육종학회지 Vol.53 No.4
This superior zoysiagrass line was selected from the zoysiagrass genetic resources collected in the Republic of Korea. Inflorescencesof Zoysia matrella lines were collected near the border between wild Zoysia japonica and wild Z. matrella on Jeju Island. Various Z. matrella× Z. japonica lines were obtained using the seeds from the collected inflorescences. In 2021, one of the lines that extended the greeningperiod during the low temperatures of winter was registered under the name of ‘Halla Green 10’. The main morphological characteristicsof this cultivar, which were analyzed using the test guidelines of the Korea Forest Service, are as follows: short length of plant (12.01±0.57cm), short length of leaf blade (8.83±0.68 cm), and short length of leaf sheath (1.71±0.20 cm). The short length of leaf sheath makes iteasy to manage the turf grass with low height. Due to the presence of trichomes on the leaf surface and medium-leaf type (leaf blade width:3.0±0.16 mm), ‘Halla Green 10’ is morphologically classified as Z. matrella × Z. japonica. As the leaf angle is very wide at 77.7°, it hasa high photosynthetic efficiency. The colors of the leaf sheath, leaf blade, and stolon are light green, green, and light purple, respectively. In particular, during the low temperature period of winter, the chlorophyll content of the leaves of ‘Halla Green 10’ shows about 7.5-foldfrom that of the control Z. japonia ‘Yaji’ and about 3.2-fold increase from that of the control Zm-6. Extending the greening period phenotypeis expected to be commercially useful (Registration No. 246).