Ascorbate Peroxidase 유전자의 도입에 의한 식물의 형질전환

이인애,이효신,배은경,김기용,이병헌,손대영,조진기 Plant molecular biology and biotechnology research 2002 Plant molecular biology and biotechnology research Vol.2002 No.-

This study was conducted to obtain the transformed tobacco (Nicotiana tubacum) plants with cytosolic ascorbate peroxidase gene(ApxSC7) using Agrobacterium tumefaciens LBA4404. A cDNA encoding the cytosolic ascorbate peroxidase of strawberry, ApxSC7, was introduced into tobacco plants via Agrobacterium-mediated gene transfer system. The expression vector, pIG-AP8, harboring ApxSC7 gene was used for production of transgenic tobacco plants. A large number of transgenic plants were regenerated on a medium containing hygromycin. Integration of ApxSC7 gene was confirmed by PCR and Southern blot analyses with genomic DNA. Northern blot analyses revealed that the pIGap8 gene was constitutively expressed.

오차드그래스(Dactylis glomerata L.)의 품종에 따른 종자유래의 캘러스 형성률과, 캘러스 크기 및 식물체 재분화 효율의 비교

배은경,이인애,김기용,이병현,손대영,이효신,정민섭,조진기 Plant molecular biology and biotechnology research 2002 Plant molecular biology and biotechnology research Vol.2002 No.-

Comparison results of callus formation ratio from seed explants, callus sizes, regeneration ratios from callus and regeneration efficiency [calculated by following formular; callus formation ratio(%) × regeneration ratio(%)/100]for 27 ochardgrass (Dactylis glomerata L.)varieties imported and Hapsung 2 developed in Korea are as follows; 1. Among ochardgrass varieties showing more than 50% callus formation ratios, the descending order of callus formation ratio after bedding the seed explants for 4 weeks was 93>Sparta>Pizza>Condor>Lidaglo>Glorus>Hapsung2>Frode. 2. The callus sizes after bedding for 4 weeks were in the range of φ 0.43cm~4.2cm in which there was 10 times size difference between the largest one and the smallest one but most of them were between φ2.5cm~4cm. 3. The regeneration ratio from callus among varieties were in the range of 0~36% and descending order of the upper 6 varieties was Plano>Akimidori>Justus>Lidacta>Currie>Hall mark. 4. The regeneration efficiency which is calculated by the ratios of regeneration from seed explant numbers was between 0 to 17.4% among which Justus showed the highest value in the 4-week treatment. 5. The correlation between callus formation ratios and the callus sizes, callus formation ratios and regeneration efficiency, and callus sizes and regeneration efficiency were r=0.5765, r=0.6365 and r=0.6246, respectively in 4-week callus and all the correlations were significant on the 1% level. 6. In 6-week callus, the descending order callus formation ratios from seed sxplants for the best 6 varieties was Condor>Sparta>93M>Justus>Potomac>Lidaglo>Frode. 7. The callus sizes formed were between φ1.5~5.7cm in which Sparta, the largest one of φ5.7cm was five times larger than the smallest one. The callus size of the control variety. Hapsung 2 was φ3.8cm, which belonged to larger size. 8. Regeneration ratio showed a great deviation among varieties from 6-week old calli by showing from 0% to 100% in which all the calli were regenerated in Plano while no callus was regenerated in Juno, 9. The range of regeneration effciency was between 0~28% among varieties in which the values from 6-week callus treatment were larger than those from 4-week callus treatment. Especially, the value of Potomac in 6-week was 3 times larger than that in 4-week. 10. The correlation between callus formation ratios and the callus sizes, callus formation ratios and regeneration efficiency, and callus sizes and regeneration effciency were r=0.8369, r=0.6683 and r=0.5937, respectively in 6-week callus, and all the correlations were significant on the 1% level.

국내육성 오차드그라스 품종들의 캘러스 형성율 및 식물체 재분화 효율

김기용,강경민,배은경,이인애,임용우,최기준,박근제,손대영,조진기 Plant molecular biology and biotechnology research 2003 Plant molecular biology and biotechnology research Vol.2003 No.-

Comparisons of callus formation ratios from seed explants, callus sizes, regeneration ratios from callus and regeneration efficiency for 4 orchardgrass (Dactylis glomerata L.) varieties (Three were developed in Korea and one was imported from foreign nation) are as follows; Jangbeol 102 (67.0%) has the highest callus formation ratio in 4 weeks incubated callus after bedding the seed explants, but Potomac (68.4%) has the highest ratio in 6-seek callus. Potomac (3.93cm) has the highest callus size in 4-week callus, but Jangbeol 101 (4.32cm) has the highest size in 6-week callus. Jangbeol 101 (17.7%) has the highest plant regeneration ratio in 4-week callus, but Potomac (37.4%) has the highest raion in 6-week callus. Jangbeol 102 (11.5%) has the highest plant regeneration efficiency in 4-week callus, but Potomac (25.6%) has the highest efficiency in 6-week callus.

Antioxidant Effect and Functional Properties of Hydrolysates Derived from Egg-White Protein

Dae Yeon Cho,Kyung Ae Jo,So Young Cho,Jin Man Kim,Kwang Sei Lim,Hyung Joo Suh,Se Jong Oh 한국축산식품학회 2014 한국축산식품학회지 Vol.34 No.3

This study utilized commercially available proteolytic enzymes to prepare egg-white protein hydrolysates (EPHs) with different degrees of hydrolysis. The antioxidant effect and functionalities of the resultant products were then investigated. Treatment with Neutrase yielded the most α-amino groups (6.52 mg/mL). Alcalase, Flavourzyme, Protamex, and Ficin showed similar degrees of α-amino group liberation (3.19-3.62 mg/mL). Neutrase treatment also resulted in the highest degree of hydrolysis (23.4%). Alcalase and Ficin treatment resulted in similar degrees of hydrolysis. All hydrolysates, except for the Flavourzyme hydrolysate, had greater radical scavenging activity than the control. The Neutrase hydrolysate showed the highest 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity (IC50=3.6 mg/mL). Therefore, Neutrase was identified as the optimal enzyme for hydrolyzing egg-white protein to yield antioxidant peptides. During Neutrase hydrolysis, the reaction rate was rapid over the first 4 h, and then subsequently declined. The IC50 value was lowest after the first hour (2.99 mg/mL). The emulsifying activity index (EAI) of EPH treated with Neutrase decreased, as the pH decreased. The EPH foaming capacity was maximal at pH 3.6, and decreased at an alkaline pH. Digestion resulted in significantly higher 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ABTS radical scavenging activity. The active peptides released from egg-white protein showed antioxidative activities on ABTS and DHHP radical. Thus, this approach may be useful for the preparation of potent antioxidant products.

경남 지역내 소 Neospora caninum에 대한 감염률 조사

박애라 ( Ae Ra Park ),하대식 ( Dae Sik Hah ),조성숙 ( Seong Suk Jo ),권영택 ( Young Taek Kwun ),박동엽 ( Dong Yeop Park ),이국천 ( Kuk Cheon Lee ),허정호 ( Jung Ho Heo ) 한국가축위생학회 2010 韓國家畜衛生學會誌 Vol.33 No.2

This survey was carried out to investigate the seroprevalence of antibodies to Neospora caninum in Korean indigenous cattle that was raised in central area province Gyeongnam, Korea. A total of 719 sera were tested for N. caninum antibodies using ELISA (Herdcheck anti-Neospora, IDEXX Laboratories Inc., Westbrook, Maine USA). Seroprevalence of individual and farm were 29.8%(214/719) and 53.2%(50/94). Regional seropositive rates of the samples were 61%(47/77), 23.3%(7/30), 13.8%(49/355), 37.6%(77/205), 65.4%(34/52) at Changwon, Jinhae, Gimhae, Miryang, Yangsan, respectively. It showed difference at the age and on the herd size of farms. A herd of cattle above the age of 5 was more infective than under 4 years. And in seroprevalence by herd size farms having under 30 heads was top(35.7%). Seropositive 214 herds of N. caninum antibidies were tested for brucellosis by test tube. Positive rate of double infection was 16.4%(35/214).

Aflatoxin에 노출된 닭에서 활성탄과 어성초의 독성완화 효과

하대식 ( Dae Sik Hah ),지대해 ( Dae Hae Ji ),조상래 ( Sang Rae Jo ),박애라 ( Ae Ra Park ),정은희 ( Eun Hee Jung ),박동엽 ( Dong Yeop Park ),이국천 ( Kuk Cheon Lee ),허정호 ( Jung Ho Heo ),김종수 ( Jong Shu Kim ) 한국가축위생학회 2010 韓國家畜衛生學會誌 Vol.33 No.2

This study was conducted to evaluate the alleviative effects of activated charcoal (AC) and Houttuynia cordata (HC) singly or in combination in broiler chickens during aflatoxicosis. Activated charcoal (1% or 0.5%) and H. cordata (1% or 0.5%) were mixed into the diets for the ability to reduced the deleterious effects of 2.4mg total aflatoxin (AFB1) kg-1 diet on growing broiler chickens from 1 to 21 days of age. A total of 160 1-day-old (Hyline Variety Brown) broiler chicks were housed in eight treatment groups [Control, AFB1, AC 1%, HC 1%, AFB1 plus AC 1% plus HC 1%, AFB1 plus AC 1% plus HC 0.5%, AFB1 plus AC 0.5% plus HC 1%, AFB1 plus AC 0.5% plus HC 0.5%] each consisting of 20 chicks. Compared to control, 2.4mg AFB1 alone treatment group significantly decreased body weight gains of chickens. The addition of mixed AC 1% and HC 1% including 6, 7 groups to the 2.4mg AFB1-containing diet moderately reduced the adverse effects of AFB1 on performances of chickens. The chickens consuming 2.4mg AFB1 plus AC 0.5% and HC 0.5%-containing diet showed very slightly reduced the adverse effects on investigated parameters compared to the AFB1 only treated group. Also, the single addition of AC or HC to the AFB1-free diet had no adverse effects in chickens. These results suggest that AC and HC mixed can reduced the aflatoxicosis in broilers and may be contribute to a solution of the aflatoxicosis problem in poultry production.

Protective effects of PEP-1-Catalase on stress-induced cellular toxicity and MPTP-induced Parkinson`s disease

( Seon Ae Eom ),( Dae Won Kim ),( Min Jea Shin ),( Eun Hee Ahn ),( Seok Young Chung ),( Eun Jeong Sohn ),( Hyo Sang Jo ),( Su Jeong Jeon ),( Duk Soo Kim ),( Hyeok Yil Kwon ),( Sung Woo Cho ),( Kyu Hyu 생화학분자생물학회(구 한국생화학분자생물학회) 2015 BMB Reports Vol.48 No.7

Parkinson’s disease (PD) is a neurodegenerative disability caused by a decrease of dopaminergic neurons in the substantia nigra (SN). Although the etiology of PD is not clear, oxidative stress is believed to lead to PD. Catalase is antioxidant enzyme which plays an active role in cells as a reactive oxygen species (ROS) scavenger. Thus, we investigated whether PEP-1-Catalase protects against 1-methyl-4-phenylpyridinium (MPP+) induced SH-SY5Y neuronal cell death and in a 1-methyl- 4-phenyl-1,2,3,6-trtrahydropyridine (MPTP) induced PD animal model. PEP-1-Catalase transduced into SH-SY5Y cells significantly protecting them against MPP+-induced death by decreasing ROS and regulating cellular survival signals including Akt, Bax, Bcl-2, and p38. Immunohistochemical analysis showed that transduced PEP-1-Catalase markedly protected against neuronal cell death in the SN in the PD animal model. Our results indicate that PEP-1-Catalase may have potential as a therapeutic agent for PD and other oxidative stress related diseases. [BMB Reports 2015; 48(7): 395-400]

Electrical Conduction Mechanism of Polypyrrole-Alginate Polymer Films

Basavaraja, C.,Jo, Eun-Ae,Kim, Bong-Seong,Kim, Dae-Gun,Huh, Do-Sung 한국고분자학회 2010 Macromolecular Research Vol.18 No.11

The polypyrrole (PPy) - alginate (Ag) blend films were synthesized using different wt% of Ag and characterized by Fourier transform infrared spectroscopy (FTIR) and ultraviolet-visible (UV-vis) spectroscopy. The morphology was examined by transmission electron microscopy (TEM). The electrical conductivity was determined by studying the I-V characteristics at temperatures ranging from 300-500 K. The results are shown by measuring the dependence of the current on the field, temperature, and blending compositions in the form of the I-V characteristics, and the analysis was made by interpretation of the Poole-Frenkel, Schottky ln (J) vs. T plots. For individual polymers, the conduction mechanism was observed to be a Poole-Frenkel type. After the polymer blend was formed, charge conduction appeared to be the Poole-Frenkel mechanism at both lower and higher temperatures. These results suggest that the Pool-Frenkel mechanism is mainly responsible for the observed conduction. The conductivity of the films increased with increasing alginate wt% in the blend and the mobility of the charge carriers increased with increasing alginate wt% concentration in the blend.

경요도적 전립선 절제술시 혈청 소디움 및 삼투질 농도에 미치는 Furosemide 의 효과

이명애,조대현,김순열 대한마취과학회 1991 Korean Journal of Anesthesiology Vol.24 No.4

This study was undertaken to compare the effects of furosemide on the serum concentration of sodium and osmolality after transurethral prostatic resection(TURP) using cytal solution, and to determine the adequate time of administration of frurosemide. At the end of prostatic resection, 15 patients were allocated randomly to receive furosemide (furosemide group) and were compared with 15 patients without administration of furosemide (control group). There was no difference in mean serum concentation of sodium between two groups. Serum osmolality in furosemide group was significantly increased as compared with control group one hour after operation. So cytal solution used during staged TURP and short operation within one hour do not affect serum corcentration of sodium and administration of furosemide is not associated with a change in serum concentration of sodium. But furosemide meaningfully increases the serum osmolality and it is more effective to administer it with administration at the end of prostatic reseetion.

대변위 측정을 위한 다중화된 광섬유 센서

유정애 ( Jung Ae Yoo ),권일범 ( Il Bum Kwon ),조재흥 ( Jae Heung Jo ),서대철 ( Dae Cheol Seo ) 한국센서학회 2005 센서학회지 Vol.14 No.3

N/A A multiplexed bend loss type single-mode fiber-optic sensor system was prepared to measure the displacement of several cm of the civil engineering structures such as many bridges, tunnels and various buildings. This bend loss type fiber-optic sensor used the signal difference between two reflection signals due to various bend losses generating at a pair of optical connectors by using OTDR (optical time domain reflectometer) for measuring displacements. And the experiments were conducted for showing the measurement feasibility on the range of 10 cm, and the multiplexing experiments were also performed to measure the displacements of 5 measuring positions of an object by setting these 5 fiber-optic sensors on a single mode fiber simultaneously.