암환자의 혈청 중 Putrescine, Spermidine 및 Spermine의 분리 정량

신태용,이석봉,김대근,채병숙 우석대학교 의약품개발연구소 1997 藥學硏究誌 Vol.2 No.-

A high performance liquid chromatographic method developed for the determination of polyamines(putrescine, spermidine and spermine) in the serum of normal human and cancer patients. The benzoyi chloride derivatives of polyamines are separated on a uBondapak C_18 reverse-phase column with methanol/water(50v/50v) as the mobile phase. The results show that the mean level of polyamines in concer patients serum is much higher than that in normal human serum.

미세혈관 협심증 환자의 임상상 및 지표에 관한 연구

박상호,온영근,김현정,이태훈,길효욱,김현건,박수진,송해정,한대희,방덕원,신원용,이내희,김성구,권영주 순천향의학연구소 2003 Journal of Soonchunhyang Medical Science Vol.9 No.1

Background : Microvascular angina or syndrom X is heterogenous and encompasses different pathogenetic entities. Several studies suggest that microvascular angina have good prognosis and occurs predominantly in postmenopausal women. Lipoprotein (a), total cholesterol, LDL-cholesterol, HDL-cholesterol, triglyceride, homocystein, CRP and fibrinogen are the independent risk factor of acute coronary syndrome and are the chief underlying cause of atherosclerosis. We investigated clinical characteristics and markers in patients with microvascular angina. Methods : We studies 23 patients diagnosed with microvascular angina and 16 control group with negative treadmill exercise test and no significant coronary angiogram. We investigated clinical characteristics in patients group and evaluated symptom during a follow-up period of 4.2± 2.5 month. Exercise time in treadmill exercise test was compared between patients and control group. We assessed the level of markers, such as lipoprotein (a), total cholesterol, LDL-cholesterol, HDL-cholesterol, triglyceride, homocystein, CRP and ESR in two groups. Results : There is no difference in age between two groups. Patients group had a tendency to increase in female, hypertension, DM, and smoking history, but there was no statistical significance difference. Exercise duration time was significantly decreased in patients with microvascular angina (p<0,05). Serum lipoprotein (a), total cholesterol, LDL-cholesterol, HDL-cholestrol, Triglyceride, ESR, Fibrinogen and Homocystein value were no significant difference between microvascular angina and control group. Only CRP was significantly high in patients with microvascular angina (p<0.05). Conclusion : There was no statistical significance difference in age, sex, hypertension, DM and smoking history between two groups but patients group had a tendency to increase the prevalence in hypertension, DM and smoking history, female. Also, Symptom improvement and disappearance in follow-up was presented in 11 patients of 19(57.9%). Exercise tolerance was worse in patients group than control group. CRP value was significantly higher in patients group than control group. There was no statistical significance difference in lipoprotein (a), total cholesterol, LDL-cholesterol, HDL-cholesterol, triglyceride, homocystein, ESR and Fibrinogen between two groups.

콜레스테롤 생합성 저해제 검색법의 개발

임종철,이남훈,김한균,염형렬,이대호,강현석,류형선,명재일,김완,신부안 全南大醫大 2001 전남의대학술지 Vol.37 No.2

The Clinical Characteristics according to the Educational Level in the Elderly Patients with Mild Alzheimer’s Disease Dementia

Dae-Seob Shin,Ho Sik Shin,Seung-Keun Lee,이동현,박정호,이태경,성기범,Kwang Ik Yang,박선아 대한치매학회 2015 Dementia and Neurocognitive Disorders Vol.14 No.4

Background and Purpose Cognitive reserve is important for the individual susceptibility to dementia. Among the various determinantsof cognitive reserve, the number of years of formal education would be of prime importance. We performed this study to explore its contributionto the clinical characteristics of dementia. Methods We included consecutive mild Alzheimer’s disease (AD) dementia patients (clinical dementia rating, CDR=0.5 or 1) who visitedour memory clinic and were older than 70 years at the evaluation from October 2013 to September 2015. According to the number of yearsof education, the corresponding subjects was grouped into two extreme educational groups, low education (illiterate or partially illiterate, LE,n=43) vs. high education (more than 9 years of education, HE, n=34). Among these patients, we compared various demographic, neuropsychologicaland neuroimaging characteristics. Results The groups were comparable in terms of age, frequency of hypertension and diabetes, and CDR and its sum of box. However, femalegender was more frequent in the LE group (p=0.000). Also this group showed a more depressive mood on the geriatric depression scale(p=0.007). The raw scores on Korean Version of Mini-Mental State Examination and well-validated neuropsychological tests were lower inthe LE group. However, the cognitive performance was found to be more preserved in the LE group than in the HE group when assessed usingthe z-score in certain specified tests on univariate and multivariate analyses (p<0.05). The Schelten’s grade of medial temporal atrophy wassimilar between the two educational groups. Also, the degree of combined ischemic burden did not differ between the two groups. Conclusions We identified that the prevalence of depressive mood and the extent of decline from the corresponding norm in a particularneuropsychological performance differ according to the educational level of mild AD patients.

Evaluation of Antioxidant Effects of Vitamins C and E Alone and in Combination with Sorghum Bran in a Cooked and Stored Chicken Sausage

Shin, Dae-Keun,Yang, Han-Sul,Min, Byoung-Rok,Narciso-Gaytan, Carlos,Sanchez-Plata, Marcos X.,Ruiz-Feria, Ciro A. Korean Society for Food Science of Animal Resource 2011 한국축산식품학회지 Vol.31 No.5

To evaluate the antioxidant effects of vitamin C, vitamin E and sorghum bran, alone or in combination on chicken sausages, 9 kg of chicken thigh meat was prepared. All thigh meat was divided into seven different batches as follows; no antioxidant (CON); vitamin C (VTC), vitamin E (VTE) or sorghum bran (SOR) at 0.02%; or three different combination ratios of vitamin C, vitamin E and sorghum bran at 0.02% (VT2, 2:1:1; VT4, 4:1:1; VT6, 6:1:1). All cooked sausages were stored at $4^{\circ}C$, and six sausages per treatment were used for chemical analysis on five different storage days. As the addition of vitamin E was increased, sausages stored for 10 d had decreased redness; thereby, VTE showed the lowest CIE $a^*$ (p<0.05). Sausages mixed with vitamins and sorghum bran combinations had lower peroxide and free fatty acid values (p<0.05) when compared to sausages without antioxidants. The TBARS were the lowest in sausages containing vitamin C, vitamin E and sorghum bran at 6:1:1 ratio, and they significantly differed to CON, VTC and SOR treatments (p<0.05). Therefore, our results suggest that meat mixed with vitamins and sorghum bran had more antioxidant activity than the meat mixed with only antioxidant vitamins or without antioxidants.

Pulsed Electric Field Effects to Reduce the Level of Campylobacter spp. in Scalder and Chiller Water during Broiler Chicken Processing

Shin, Dae-Keun,Martin, Bradely C.,Sanchez-Plata, Marcos X. Asian Australasian Association of Animal Productio 2011 Animal Bioscience Vol.24 No.9

To evaluate the effects of pulsed electric field (PEF) application on scalder and chiller water on Campylobacter contamination, four different treatments under three different water conditions including hard scalder water ($55^{\circ}C$), soft scalder water ($45^{\circ}C$) and chiller water, were applied as follows: i) a control treatment with no salt and no electric treatment, ii) a PEF only treatment, iii) a PEF treatment with 0.5% salt water, and iv) a PEF treatment with 1% salt water treatment. The use of PEF in hard scalding water showed an effect of reducing Campylobacter when compared to the control during the 200 s timeframe. With the addition of salt, the intervention caused at least 5.81 log CFU/ml reduction of Campylobacter counts after 200 s of PEF exposure. Similar effects were observed under soft scalding conditions. Campylobacter reductions were evident under chilling conditions with up to 2.00 log for PEF only, 5.77 log for PEF+0.5% salt and 2.69 log for PEF+1% salt treatment in water. Therefore, the current PEF setting for the scalder and chiller water can be successfully used to reduce pathogenic loads of Campylobacter on broiler chicken carcasses, and further research may be necessary to apply it in the poultry processing industry.

Reconfiguration of the Global Value Chain in Response to the U.S.-South Korea-Japan Triangular Cooperation and Policy Implication

Dae Keun SHIN(신대근) 산업통상자원부 2023 통상법무정책 Vol.- No.6

To secure national interests, it is critical to promote advanced, high-tech industries, thus evolving into an innovative manufacturing leader. Strategic asset development in these sectors will enable a strong supply chain, leveraging our strengths to meet current global economic challenges. Chinas Semiconductor Ambition and its Made in China 2025 goal for 70% semiconductor self-sufficiency by 2025, backed by substantial investment, exemplifies such challenges. In response, the U.S. has implemented policies to diminish dependence on Chinas supply chain. The Biden administrations executive order and subsequent legislation underscore this, aiming to boost domestic production and establish the Chip 4 alliance with South Korea, Japan, and Taiwan, deliberately excluding China. The tripartite consensus between the U.S., South Korea, and Japan in August 2023 to establish an independent supply chain is significant. Collaborating in advanced technology sectors, they aim to create a counterbalance to Chinas influence, drawing on their combined economic might and technological expertise. However, the potential for economic retaliation from China could impact South Korea, raising the stakes for this cooperation. Analyzing the political and economic backdrop of this alliance is essential, as is exploring diverse policies to reduce the supply chain risk linked to China. This strategic collaboration and risk assessment are crucial in navigating the complex international landscape.

Legal aspect of the Value - oriented Trade Regime from the Multilateral Perspective and Policy Responses - With respect to the CBAM and IRA

Shin Dae-Keun(신대근) 산업통상자원부 2022 통상법무정책 Vol.- No.4

The crisis of the multilateral system has been raised for a long time. In particular, uncertainties surrounding the global trade environment are increasing such as the US-China dispute and reorganization of supply chains. Unfortunately, the restoration of the 1995 WTO system based on multilateralism is not likely to happen in a foreseeable future. Economic powerhouses such as the U.S. and China are not hesitant to use trade policies as a tool to maximize their national interests. Against the backdrop of increasing uncertainty, non-market values such as climate change and human rights have begun to be merged with international trade. A new type of trade regime centered on countries or regions is being formed. Two concrete measures embodied with the value-oriented trade policies are the EUs Carbon Border Adjustment System(CBAM) and the U.S. Inflation Reduction Act(IRA). Although it is a bit far-fetched to compare both on the equal footing due to their structural difference, both ultimately have a common point in establishing the foundation of value-oriented trade regime. The advocates of both CBAM and IRA put forth the protection of universal values such as the environment, human rights, and democracy as a means to justify their deviation from multilateral protocols. Nevertheless, both run the risk of being exploited as a tool for their national interest. Since both can be abused as a disguised restriction on international trade prohibited under the multilateralism, it is very important to accurately diagnose their potential ramifications. This paper will review the policy and legal issues of both measures and examine their compatibility with the WTO rules from a multilateral perspective. Based on the analysis, policy proposal for both government and private sector will also be reviewed.

지역별 통상진흥기관협의회를 통한 중소기업의 통상대응 역량 강화

신대근 ( Shin Dae Keun ) 산업통상자원부 2023 통상법무정책 Vol.- No.5

The government represented by the Ministry of Trade, Industry, and Energy has been striving to secure a continuous market and create growth energy through active promotion of Free Trade Agreements (FTAs), considering our economic structure as an open trade country. To support the utilization of FTAs signed with foreign countries by small and medium-sized companies across the country, the government has established and operated FTA comprehensive support centers and 18 regional FTA utilization support centers based on the Ministry of Industry's order. However, the existing FTA utilization policy that focuses on the use of preferential tariffs has limitations in the rapidly changing trade environment. Therefore, the Ministry of Trade, Industry and Energy launched a pan-ministerial consultation to help companies adapt to the changing trade environment. The Trade Promotion Agency Council aims to resolve difficulties faced by small and medium-sized companies when exporting by receiving non-tariff barriers, reflecting them in trade negotiations and policies with foreign countries. The Council will act as a communication channel by explaining the latest trends and responses from companies on various issues. This pan-ministerial trade promotion agency council aims to differentiate from existing local government-centered export support policies, and it has current implementation plans and future plans. The regional trade promotion agency council, launched under this agreement, is expected to play a significant role in overcoming the crisis of small and medium-sized companies vulnerable to changes in the trade environment amid growing uncertainties in external economic conditions, such as protectionism.

Cytotoxic Constituents of Sorbaria sorbifolia var. stellipila

Kim, Dae-Keun,Choi, Sang-Hoon,Lee, Jung-Ock,Ryu, Shi-Yong,Park, Dae-Kyu,Shin, Dae-Hee,Jung, Jee-Hyung,Pyo, Suhk-Keung,Lee, Kang-Ro,Zee, Ok-Pyo The Pharmaceutical Society of Korea 1997 Archives of Pharmacal Research Vol.20 No.1

The activity-guided fractionation upon the MeOH extract of the aerial parts of Sorbaria sorbifolia var. stellipila led to the isolation of two cucurbitacin-compounds, cucurbitacin D and cucurbitacin F, as active principles. Two compounds were shown to exhibit significant cytotoxicity against cultured human tumor cell lines, A-549, SK-OV-3, SK-MEL-2, XF-498, and HCT 15.