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DY Hwang,JH oh,YK Kim,SB Shim,SW Jee,SH Lee,SJ Seo,YS Song,KT Nam,JY Cho,Jane Hwang,IS Jang,JS Cho 한국실험동물학회 2005 Laboratory Animal Research Vol.21 No.2
Type 2 or noninsulin-dependent diabetes mellitus accounts for over 90% of cases and is characterized by a triad of resistance to insulin action on glucose uptake in peripheral tissues, impaired insulin action to inhibit hepatic glucose production, and dysregulated insulin secretion. To determine whether a transgene-based small interfering RNA (siRNA) for insulin and an overexpression of human insulin degrading enzyme (hIDE) decrease the insulin level in vitro and in vivo or not, several siRNA sequences and hIDE gene for insulin were overexpressed in the insulinoma cells and mouse via tail vein. The siRNAs for insulin suppression were very effective at the regions of 88-99 bp and 109-131 bp in rat insulin sequence. Insulin suppression by the specific siRNA sequences and hIDE significantly induced endoplasmic reticulumn (ER) stress and decreased insulin receptor a expression in insulinoma cells. The insulin level in plasma significantly decreased by overexpressions of the siRNAs and hIDE gene in mouse, which may be caused by the degradation of insulin due to overexpression of hIDE protein in mouse liver. These results suggest that this plasmid for the suppression of insulin secretion by the specific siRNAs and the insulin degradation by an overexpression of hIDE protein may be useful on the development of animal model for Type 2 diabetes.
Methotrexate투여로 치유한 자궁경관임신 1 예
황도영(DY Hwang),최진(J Choi),심상덕(SD Shim),서대식(DS Suh),최영민(YM Choi),김승욱(SW Kim) 대한산부인과학회 1992 Obstetrics & Gynecology Science Vol.35 No.3
Cervical pregnancy is a rare form of ectopic gestation in which the fertilized ovum implants within the cervical canal below the internal os of uterine cervix. The etiology is not clearly identified, but the surgical damage such as abortion and multiparity is suspected. In the management of cervical pregnancy, hysterectomyb usually was done because of profuse vaginal bleeding. However, the attempts to preserve the uterus have been tried after 1960`s. The use of methotrexate in the management of ectopic pregnancy was introduced by Tanaka et al in 1982 and the treatment of cervical pregnancy with methotrexate may be a worthwhile trial for the preservation of the uterus. We present a case of cervical pregnancy treated with methotrexate and leucovorin, and the literatures on cervical pregnancy were briefly reviewed.
Hwang, SY,Cho, SH,Cho, DY,Lee, M,Choo, J,Jung, KH,Maeng, JH,Chai, YG,Yoon, WJ,Lee, EK Informa Healthcare 2011 Biotechnic & histochemistry Vol.86 No.3
<P>Apoptosis is an important phenomenon for investigating the efficacy of anti-cancer drug candidates. The conventional assays for cellular apoptosis, such as enzyme-linked immunosorbent assay, absorbance monitoring for the activity of caspase, and flow cytometric assay, have focused only on biochemical events. We investigated the staurosporine (STS)-induced apoptosis of the murine macrophage RAW-264.7 cell using a cell based bioimaging technique. Using time-lapse confocal microscopy, we monitored caspase-3 activation during apoptosis by imaging the translocation of green fluorescent protein from the cytosol to the nuclei. Five hours after 1 μ關M STS treatment, caspase-3 was observed to be activated and membrane blebbing was observed simultaneously. Also, the loss of phosphatidylserine (PS) asymmetry in the phospholipid bilayer of plasma membrane during early apoptosis was monitored by imaging annexin-V labeled with fluorescein isocyanate binding to the externalized PS at various concentrations of STS. Moreover, disintegration of the plasma membrane during late apoptosis was confirmed using a nuclear dye, propidium iodide. The single cell based bioimaging data agreed well with those of the biochemical assays for caspase activation and morphological observation for membrane integrity.</P>
중합효소연쇄반응을 이용한 한국인 혈우병 A 환자의 유전자내 BclI 및 XbaI RFLP 양상에 관한 연구
최영민(YM Choi),황도영(DY Hwang),최진(J Choi),구승엽(SY Koo),김석현(SH Kim),신창재(CJ Shin),김정구(JK Kim),문신용(SY Moon),이진용(JY Lee),장윤석(YS Chang),안효섭(HS Ahn),김은주(EJ Kim) 대한산부인과학회 1994 Obstetrics & Gynecology Science Vol.37 No.12
Hemophilia A is one of the most common inherited bleeding disoders in humans, resulting from thterogenous colletion of mutations in the factor VIII gene. With the molecular clonig of the human gene for factor VIII, intragenic polymorphic DNA sequeces have been described and these sequeces are currently used for accurate identfication of carriers and for prenatal diagnosis, usually by radioactive Southern blot analysis which is costly and labor intensive. The recent development of polymerase chain reaction provides a more rapid and nonradioactive technique for the carrier-detection and prenatal diagnosis. To identify the clinically useful markers that can be used for the carrier-detection and prenatal diagnosis, we analysed the BclI and XbaI intragenic polymorphism of factor VIII gene in 23 Korean patients with hemophilia A and their mothers using polymerase chain reaction(PCR). BclI intragenic polymorphism was informative in 4(17.4%) famlies among 23 families tested, whie XbaI intragenic polymorphism was informative in one (4.3%) family among those familes. In some mothers of the patient, anaylsis of XbaI polymorphism was not possible by digestion because of the background bands that interfere with interpretation. These data indicated that PCR-based analysis of BclI intragenic polymorphism is a clinically useful method for the carrier-detection and for prenatal diagnosis of hemophilia A in Korean patients.
이광재(KJ Lee),윤대영(DY Yun),문시영(SY Moon),황상의(SU Hwang) 대한산부인과학회 1982 Obstetrics & Gynecology Science Vol.25 No.1
저자들은 1979년 5월 부산 성분도병원 산부인과에서 수술후 병리학적으로 확인된 원발성 난관암을 경험하였기에 문헌고찰과 함께 보고하는 바이다. Primary carcinoma of the fallopian tube is very rare female genital cancers. A case of primary papillary alveolar type carcinoma of the left fallopian tube was found postoperatively in 46 years old premenopausal women with nulliparity. A brief review of literature is added.
한국인 취약 X증후군 환자 및 보인자의 분자유전학적 양상과 진단
최영민(YM Choi),황도영(DY Hwang),최진(J Choi),박선희(SH Park),김석현(SH Kim),양세원(SW Yang),조수철(SC Cho),문신용(SY Moon),이진용(JY Lee) 대한산부인과학회 1996 Obstetrics & Gynecology Science Vol.39 No.5
Fagile X syndrome is the most common cause of inherited mental retardation. The diagnosis of fragile X syndrome was originally based on the expression of a folate-sensitive fragile site at X chromosome(Xq27.3) in cell culture under conditions of folate deprivation. The cytogenetic study test has limitations, especially in testing for carrier status, and it exhibites a high degree of variability between individuals and laboratories. In 1991, the fragile X gene(FMR-1) was characterized and the nature of the fragile X mutation has been elucidated. At present in developed countries, DNA testing has been utilized in the diagnosis of fragile X syndrome and its carriers. This study was undertaken to analysis the FMR-1 gene in Korean patients with fragile X syndrome and tis carriers and to establish the methodology for molecular genetic diagnosis of fragile X syndrome and its carrier. Three male patients with fragile X syndrome previously diagnosed by cytogenetic analysis and their family members, as well as 16 persons proven cytogenetically normal were analysed by direct genomic Southern blot anlysis and polymerase chain reaction (PCR) for the status of FMR-1 gene. Southern blot analysis consisted of double DNA digestion with Eag I and EcoRI and the hybridization whth StB 12.3 probe. Two kinds of PCR methods were used. The one method involved the addition of radio-active dNTP in PCR reaction, and the other involved Southern trnsfer of amplified PCR products and the hybridization analysis with synthetic oligonucletide probe. In normal female and male, firect genomic Southern blot analysis revealed the patterns as follows: 2.8 kb band in normla male, and 2.8kb band in normal female. And the patients(male) with fragile X syndrome showed the band sized more than 5.8 kb with 5.2 band. The father of a patient with fragile X syndrome showed the normal pattern. PCR analysis of the cytogeneticaly normal males and females revleaed the normal patterns, namely the bands of sizes expected from the primers used. However, either PCR method failed to reveal full mutation alleles in patients with fragile X syndrome. And premutation alleles could be detected in two mothers of patients by PCR method. The father of a patient with fragile X syndrome showed the normal pattern by either PCR method. Thses data suggest that Southern blot analysis can be used in the accurate diagnosis of fragile X syndrome and its carrier and that PCR method can be utilized to exclude the diagnosis of fragile X syndrome in unaffected males.