MSP58 Knockdown Inhibits the Proliferation of Esophageal Squamous Cell Carcinoma in Vitro and in Vivo

Xu, Chun-Sheng,Zheng, Jian-Yong,Zhang, Hai-Long,Zhao, Hua-Dong,Zhang, Jing,Wu, Guo-Qiang,Wu, Lin,Wang, Qing,Wang, Wei-Zhong,Zhang, Jian Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.7

Esophageal carcinoma (EC) is one of the most aggressive cancers with a poor prognosis. Understanding the molecular mechanisms underlying esophageal cancer progression is a high priority for improved EC diagnosis and prognosis. Recently, MSP58 was shown to behave as an oncogene in colorectal carcinomas and gliomas. However, little is known about its function in esophageal carcinomas. We therefore examined the effects of MSP58 knockdown on the growth of esophageal squamous cell carcinoma (ESCC) cells in vitro and in vivo in order to gain a better understanding of its potential as a tumor therapeutic target. We employed lentiviral-mediated small hairpin RNA (shRNA) to knock down the expression of MSP58 in the ESCC cell lines Eca-109 and EC9706 and demonstrated inhibition of ESCC cell proliferation and colony formation in vitro. Furthermore, flow cytometry and western blot analyses revealed that MSP58 depletion induced cell cycle arrest by regulating the expression of P21, CDK4 and cyclin D1. Notably, the downregulation of MSP58 significantly inhibited the growth of ESCC xenografts in nude mice. Our results suggest that MSP58 may play an important role in ESCC progression.

Screening and Cloning of RAPD Markers from the W Chromosome of Silkworm, Bombyx mori L.

( Chun Xia Zhang ),( Qin Yao ),( Qing Gang Xu ),( Xu Dong Tang ),( Ke Ping Chen ) 한국잠사학회 2007 International Journal of Industrial Entomology Vol.8 No.2

Silkworms sex determination drew high attention from researchers. Sex chromosomes on the silkworm are of ZW type for females and ZZ type for males. Chromosome W plays an important role in sex determination. Although several molecular linkage maps have been constructed for silkworm, very few markers are discovered on the W chromosome. In order to look for molecular markers and to further locate the Fem gene on chromosome W, we used genomic DNA from both female and male larvae of a silkworm strain named 937 as PCR templates for RAPD amplification with 200 arbitrary 10-mer primers. The amplification results showed three female-specific bands, namely OPG-07(496), OPC-15(1,660) and OPE-18(1,279). Further verification, however, revealed no band from OPG-07 and OPC-15 in either sex in the strain 798, but OPE-18 provided female-specific band in the strains Suluan7 and C108, and absent in both males and strain 798. This indicates that the bands from OPG-07(496) and OPC-15(1,660) are probably female-specific in strain 937, and the band from OPE-18 was probably amplified from a common segment shared by most strains. The genomic DNAs from OPG-07 and OPC-15 were cloned and sequenced. Sequence analysis showed that the DNAs from OPG-07 and OPC-15 have high identities with the retrotransposable elements, and DNA from OPC-15 contains a portion of sequence which probably encodes an eukaryotic translation initiation factor 4E binding protein (eIF4EBP).

Selection signature reveals genes associated with susceptibility loci affecting respiratory disease due to pleiotropic and hitchhiking effect in Chinese indigenous pigs

Xu, Zhong,Sun, Hao,Zhang, Zhe,Zhang, Cheng-Yue,Zhao, Qing-bo,Xiao, Qian,Olasege, Babatunde Shittu,Ma, Pei-Pei,Zhang, Xiang-Zhe,Wang, Qi-Shan,Pan, Yu-Chun Asian Australasian Association of Animal Productio 2020 Animal Bioscience Vol.33 No.2

Objective: Porcine respiratory disease is one of the most important health problems causing significant economic losses. To understand the genetic basis for susceptibility to swine enzootic pneumonia (EP) in pigs, we detected 102,809 single nucleotide polymorphisms in a total of 249 individuals based on genome-wide sequencing data. Methods: Genome comparison of susceptibility to swine EP in three pig breeds (Jinhua, Erhualian, and Meishan) with two western lines that are considered more resistant (Duroc and Landrace) using cross-population extended haplotype homozygosity and F-statistic (F_ST) statistical approaches identified 691 positively selected genes. Based on quantitative trait loci, gene ontology terms and literature search, we selected 14 candidate genes that have convincible biological functions associated with swine EP or human asthma. Results: Most of these genes were tested by several methods including transcription analysis and candidate genes association study. Among these genes: cytochrome P450 1A1 and catenin beta 1 (CTNNB1) are involved in fertility; transforming growth factor beta receptor 3 plays a role in meat quality traits; Wnt family member 2, CTNNB1 and transcription factor 7 take part in adipogenesis and fat deposition simultaneously; plasminogen activator, urokinase receptor (completely linked to AXL receptor tyrosine kinase, r² = 1) plays an essential role in the successful ovulation of matured oocytes in pigs; colipase like 2 (strongly linked to SAM pointed domain containing ETS transcription factor, r² = 0.848) is involved in male fertility. Conclusion: These adverse genes susceptible to swine EP may be selected while selecting for economic traits (especially reproduction traits) due to pleiotropic and hitchhiking effect of linked genes. Our study provided a completely new point of view to understand the genetic basis for susceptibility or resistance to swine EP in pigs thereby, provides insight for designing sustainable breed selection programs. Finally, the candidate genes are crucial due to their potential roles in respiratory diseases in a large number of species, including human.

Network Media Communication System of the Security Technology

Zhang, Chun-Xu,Shin, Yun-Ho Korea Institute of Electronic Communication Scienc 2008 한국전자통신학회 논문지 Vol.3 No.1

There are multiple reasons that caused the present serious status of network security, including Internet itself having a weak basis. However security is usually discarded when it contends with performance. As the world becomes more tightly interconnected, security technology continues to mature, organizations are feeling a greater need to rediscover network security. Network security technology generally concentrates on protection of the network infrastructure and, by implication, the protection of the user. This is a paper, describe current problems of network security and propose solutions.

The Analysis on DSP-based hands-free car kit

Zhang, Chun-Xu,Shin, Yun-Ho,Shin, Hyun-Sik Korea Institute of Electronic Communication Scienc 2008 한국전자통신학회 논문지 Vol.3 No.4

For the past several years, many countries have passed or have recommended legislation making it illegal to use in-hand mobile phones while driving and several manufacturers have released car kit solutions enabling hands-free operation of the mobile phone. But an automobile environment can pose extremely harsh physical conditions impacting audio quality, safety, and reliability. This article introduced a Car Kits that provided a total entertainment and telematics solution, which support all current features within the constraints of low power consumption, form factor, price, ease-of-use, manufacture ability, testability and high total quality.

Overview and Development of Digital SignalProcessing

Zhang, Chun-Xu,Shin, Yun-Ho Korea Institute of Electronic Communication Scienc 2008 한국전자통신학회 논문지 Vol.3 No.2

Digital signal processing (DSP) is the process of taking a signal and performing an algorithm on it to analyze, modify, or better identify that signal.[1] To take advantage of DSP advances, one must have at least a basic understanding of DSP theory along with an understanding of the hardware architecture designed to support these new advances. There are several programming techniques that maximize the efficiency of the DSP hardware, as well as a few fundamental concepts used to implement DSP software. This article introduced some of these underlying functions that are the building blocks of complex signal processing functions, and It will touch on the fundamental concepts of DSP theory and algorithms and also provide an overview of the implementation and optimization of DSP software, and discuss the development of DSP.

The Analysis on DSP-based hands-free car kit

Chun-xu Zhang,Yun-ho Shin,Hyun-sik Shin 한국전자통신학회 2008 한국전자통신학회 논문지 Vol.3 No.4

For the past several years, many countries have passed or have recommended legislation making it illegal to use in-hand mobile phones while driving and several manufacturers have released car kit solutions enabling hands-free operation of the mobile phone. But an automobile environment can pose extremely harsh physical conditions impacting audio quality, safety, and reliability. This article introduced a Car Kits that provided a total entertainment and telematics solution, which support all current features within the constraints of low power consumption, form factor, price, ease-of-use, manufacture ability, testability and high total quality.

Network Media Communication System of the Security Technology

Chun-xu Zhang,Yun-ho Shin 한국전자통신학회 2008 한국전자통신학회 논문지 Vol.3 No.1

There are multiple reasons that caused the present serious status of network security, including Internet itself having a weak basis. However security is usually discarded when it contends with performance. As the world becomes more tightly interconnected, security technology continues to mature, organizations are feeling a greater need to rediscover network security. Network security technology generally concentrates on protection of the network infrastructure and, by implication, the protection of the user. This is a paper, describe current problems of network security and propose solutions.

Overview and Development of Digital SignalProcessing

Chun-xu Zhang,Yun-ho Shin 한국전자통신학회 2008 한국전자통신학회 논문지 Vol.3 No.2

Digital signal processing (DSP) is the process of taking a signal and performing an algorithm on it to analyze, modify, or better identify that signal.[1] To take advantage of DSP advances, one must have at least a basic understanding of DSP theory along with an understanding of the hardware architecture designed to support these new advances. There are several programming techniques that maximize the efficiency of the DSP hardware, as well as a few fundamental concepts used to implement DSP software. This article introduced some of these underlying functions that are the building blocks of complex signal processing functions, and It will touch on the fundamental concepts of DSP theory and algorithms and also provide an overview of the implementation and optimization of DSP software,and discuss the development of DSP

RNAi suppression of nuclear receptor genes results in increased susceptibility to sulfoxaflor in brown planthopper, Nilaparvata lugens

Lu Xu,Chun-Qing Zhao,De-Jin Xu,Guang-Chun Xu,Xiao-Long Xu,Zhao-Jun Han,Ya-Nan Zhang,Zhong-Yan Gu 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.2

Nuclear receptors (NRs), which belong to a superfamily of ligand-dependent transcription factors, play essential roles in gene regulation to affect numerous physiological pathways. Twenty NR genes were identified in Nilaparvata lugens by using genomic, transcriptomic and GenBank databases, and categorized into NR0-NR6 subfamilies according to standard nomenclature. Among them, three NR geneswere classed into NR0, four into NR1, eight into NR2, one into NR3, one into NR4, two into NR5, and one into NR6, respectively. A phylogenetic tree of NRs from N. lugens and other representative species was constructed, which provided evolutionary insight into genetic distance. In order to investigate the NRs,whichwere induced by sulfoxaflor, time- and tissue-specific expression profiles of NR genes in fourth-instar nymphs were determined following LD50 sulfoxaflor treatment (0.28 ng/insect) and compared with control samples. NlUSP, NlE78, NlTLL, NlHR51, NlHR83, NlPNR and NlFTZ-F1 were highly expressed following 12–48 h of sulfoxaflor treatment. NlHR39 and NlDSF transcripts were detected in the head and the thorax, respectively. High-level and constitutive expression of NlHR3, NlTLL, NlHR83, NlFTZF1 and NlHR4 were found in the abdomen. Feeding of dsRNAs reduced the expression of NlHR3, NlUSP, NlTLL, NlHR83, NlPNR, NlFTZ-F1 and NlHR4 (35.48–49.77%) and caused significant nymph mortality (69.21–81.45%). These NRs, considered as insecticide targets, may play important function in sulfoxaflor detoxification.