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Chua Kien Hui,Wan Kamarul Zaman Wan Safwani,Seah Shiao Chin,Annisaa Abu Samah Abdul Malek,Noormazita Hassan,Muhamad Syakeer Fazil,Raja Abdul Wafy Raja Muhammad Rooshdi,Adila A. Hamid,Somasundaram Sath 한국생물공학회 2012 Biotechnology and Bioprocess Engineering Vol.17 No.6
Recently human adipose-derived stem cells (ASCs) have shown much therapeutic potential in regenerative medicine. However, fetal bovine serum (FBS)used in culturing human cells may give risk to viral and prion transmission as well as immune rejection. Human serum (HS) is a safer growth supplement in human cell culture but its effects have not been well established. Therefore the objectives of this study were to compare the effects of HS versus FBS on the proliferation and stemness gene expression of ASCs. ASCs were cultured for 5passages in medium supplemented with either 10% HS or 10% FBS. ASCs proliferation rate and viability were determined at every passage. Total RNA was extracted at passage 5 (P5) and quantitative PCR was carried out to determine the stemness gene expression level of SOX-2,Nanog3, BST-1, REX-1, ABCG2 and FGF-4. The results showed ASC cultured in 10% HS scored greater proliferation rates and viability compared to 10% FBS. ASCs proliferated significantly faster in 10% HS compared to 10% FBS at P2, P3, and P4 (p < 0.05). In quantitative gene expression analysis, ASCs cultured in 10% FBS showed a significant increase of BST-1, REX-1 and ABCG2 expression compared to 10% HS. In conclusion, HS promotes ASCs proliferation and viability but its ability to support the stemness property of ASCs was inferior to FBS.
East Asian Pop Culture: Consumer Communities and Politics of the National
Chua Beng Huat 한국방송공사 2006 방송 문화 연구 Vol.18 No.1
At the beginning of the 21st century, the global arrival of East Asian pop cultures is most evident in the popularity of commercial Chinese and Korean cinemas. The success of Crouching Tiger, Hidden Dragon (2000) spawned a string of big budget wuxia(武俠) films from globally renowned Chinese directors, often with a pan-East Asian cast of Chinese, Japanese and Korean stars. Since 2000, the continuous flow of Korean films into the global market constitutes part of what has come to be known as the ‘Korean Wave’. At the regional level, the popularity of Japanese and Korean TV dramas have been used by newly established local TV stations in the rest of East Asia, as the vehicles to capture very significant shares of local audiences. The cross-border circulation of East Asian pop cultures has generated pan-East Asian consumer communities, ranging from avid fan clubs to geographically dispersed leisure consumers who are only vaguely aware of their ‘membership’ in the communities of consumers. Conversely, the popularity of border-crossing East Asian pop cultures has also generated local reactions against the ‘invasion’ of imported cultures, couched in xenophobic, nationalist cultural rhetoric. These reactions constitute part of the larger politics of the ‘popular’ of the specific East Asian location in question.
Obstructive fibrinous pseudomembrane tracheitis after double-lumen tube intubation -a case report-
Chua Jia-Hui,Sim Brenda Ling Hui,Theng Tina Koh Puay,Chew Sophia 대한마취통증의학회 2022 Korean Journal of Anesthesiology Vol.75 No.4
Background: Obstructive fibrinous pseudomembrane tracheitis (OFPT) is a rare complication of endotracheal intubation. Case: We describe the case of a 73-year-old woman who underwent short-term intubation for video-assisted thoracoscopic surgery and developed an acute life-threatening stridor two days after extubation. The patient required an emergency tracheostomy to maintain airway patency and a microscopic direct laryngoscopy procedure was performed thereafter with removal of the obstructive pseudomembrane. Subsequently, the patient also suffered a non-ST-elevation myocardial infarction. The patient successfully recovered, and the tracheostomy was subsequently decannulated two months later. Histological examination revealed mucosal ulcerations and inflammatory changes.Conclusions: OFPT is an uncommon cause of life-threatening airway obstruction after extubation that is not often recognized immediately but can usually be treated with early bronchoscopic intervention or microscopic direct laryngoscopy.