완전뇌허혈후 재관류시에 국소뇌혈류, 체성감각유발전위 및 신경학적 회복에 대한 Mannitol과 Thiopental의 효과

박춘근,이상원,박영섭,최승진,허필우,정동섭,강준기,최창락 대한신경외과학회 1996 Journal of Korean neurosurgical society Vol.25 No.2

The cardiopulmonary bypass or cerebral circulation arrest is often used in the treatment of complex aneurysm or of arteriovenous malformation to decrease the risk of intraoperative aneurysm rupture. Although experimental studies have suggested that some drugs may protect the brain from ischemic injury, there are limitations in maintaining cerebral perfusion arrest without incurring neurologic deficits due to the initiation of detrimental processes including excitotoxic neuronal injury, activation of phospholipases, influx of calcium, and generation of damaging free radicals. The purpose of this study is to determine wheter mannitol or thiopental has any favorable effects on the recovery of neurologic deficits and on the regional cerebral blood flow(rCBF), somatosensory evoked potential(SEP) and electroencephalogram(EEG) in cats which underwent 15 minutes-complete global ishcemia-reperfusion. The complete global ischemia was produced in 38 cats by temporary intrathoracic occlusion of the innominate artery and the subclavian artery following ligation of bilateral mammary arteries and simultaneous induction of hypotension. The cats were allocated randomly to one of 4 treatment groups : (1) control group, 8 cats received equal volume of saline solution : (2) thiopental group, 10 cats received 45㎎/㎏ thiopental intravenously, (3) mannitol group, 10 cats received 2g/㎏ mannitol intravenously, (4) combined mannitol and thiopental group, 10 cats received equal dose of mannitol and thiopental intravenously. The drugs were administrated in a equally divided dosage before and after the ischemic episode. The results were as follos : 1) Eight animals which received saline showed the severe postischemic hypoperfusion and poor recovery of SEP and EEG, and 6 of them died within 6 hours after the ischemia. 2) Ten thiopental-treated animals also showed the severe postischemic hypoperfusion and poor recovery of SEP and EEG, and 6 of them died within 6 hours the ischemia. 3) Both mannitol-and combined treated groups showed early recovery of EEG, good recovery of SEP and EEG without the severe postischemic hypoperfusion, and 7 of 10 mannitol-treated animals and 8 of 10 combined treated animals were significantly recovered in all parameters. There were not significant differences in all parameters between the mannitol-and combined treated groups. 4) Thirty-four of 38 animals involved in this 15 minutes-ischemia resulted in the severe neurologic deficits inspite of treatment with mannitol, thiopental or both of them. These results suggest that, in cats, mannitol treatment is effective but not thiopental in preventing severe neurologic injury following complete global ischemia and the duration of complete ischemia should be far less than 15 minutes.

下氣道感染性疾患에서의 Sultamicillin 臨床硏究

朴春植,金建烈 최신의학사 1986 最新醫學 Vol.29 No.4

Lung Compliance(Effective)에 關한 硏究 : ARDS에서의 豫後判定指標를 中心으로 in Particular reference to Prognostic parameter

金建烈,朴春植 최신의학사 1983 最新醫學 Vol.26 No.6

전방 또는 전측방 흉수 종양 수술에서 후측방도달법

조경석,박춘근,성재훈,허필우,박춘근,백민우,김달수,강준기 대한신경외과학회 1997 Journal of Korean neurosurgical society Vol.26 No.5

유한요소법을 이용한 기둥형 로드셀의 비선형성 평가

배준우,황재근,박경흠,양희준,이춘열 嶺南大學校 工業技術硏究所 2004 工業技術硏究所論文集 Vol.32 No.1

Recently, force measurement systems are commonly used in many industrial fields and the precision of the measurement system is getting more important as the industry needs more precise tools and instruments to make high quality products. However, a high precision force measurement system is hard to make unless we know precisely the causes, quality and quantity of measurement errors in advance. In this work, many possible mechanical causes of measurement errors are reviewed including ratio of length to diameter of sensing part, radius of contact area, radius of bearing part, ratio of material properties and change of boundary conditions. Also, the measurement errors are analyzed by nonlinear finite element method and the nonlinear behavior of the errors are investigated. The results can be used to design force measurement systems and expected to be very useful especially for compact type load cells.

추적관찰 중 종괴의 크기가 커진 경화성 혈관종 1례

안형수,장안수,이준혁,박성우,고은석,백상현,박재성,허균,신화균,박춘식 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.2

We report a case of a sclerosing hemangioma of the lung with increased size during follow up, a rare benign neoplasm of the lung. A 32-year-old woman had round increased opacity in right upper lobe on posteroanterior chest radiograph. That lesion has grown slowly over four years. Computed tomographic scan showed a well circumscribed lung mass in medial aspect of right upper lobe. Radiological differential diagnosis for the lesion included inflammatory pseudotumor, atypical carcinoid, lung cancer and localized fibrous tumor of pleura. Nonspecific findings were shown by the bronchoscopy and percutaneous transthoracic needle aspiration. Anterior and apical segmentectomy was carried out and the lesion was pathologically diagnosed as sclerosing hemangioma of lung.

Development of Models for Regional Cardiac Surgery Centers

Park, Choon Seon,Park, Nam Hee,Sim, Sung Bo,Yun, Sang Cheol,Ahn, Hye Mi,Kim, Myunghwa,Choi, Ji Suk,Kim, Myo Jeong,Kim, Hyunsu,Chee, Hyun Keun,Oh, Sanggi,Kang, Shinkwang,Lee, Sok-Goo,Shin, Jun Ho,Kim, The Korean Society for Thoracic and Cardiovascular 2016 Journal of Chest Surgery (J Chest Surg) Vol.49 No.1

Background: This study aimed to develop the models for regional cardiac surgery centers, which take regional characteristics into consideration, as a policy measure that could alleviate the concentration of cardiac surgery in the metropolitan area and enhance the accessibility for patients who reside in the regions. Methods: To develop the models and set standards for the necessary personnel and facilities for the initial management plan, we held workshops, debates, and conference meetings with various experts. Results: After partitioning the plan into two parts (the operational autonomy and the functional comprehensiveness), three models were developed: the 'independent regional cardiac surgery center' model, the 'satellite cardiac surgery center within hospitals' model, and the 'extended cardiac surgery department within hospitals' model. Proposals on personnel and facility management for each of the models were also presented. A regional cardiac surgery center model that could be applied to each treatment area was proposed, which was developed based on the anticipated demand for cardiac surgery. The independent model or the satellite model was proposed for Chungcheong, Jeolla, North Gyeongsang, and South Gyeongsang area, where more than 500 cardiac surgeries are performed annually. The extended model was proposed as most effective for the Gangwon and Jeju area, where more than 200 cardiac surgeries are performed annually. Conclusion: The operation of regional cardiac surgery centers with high caliber professionals and quality resources such as optimal equipment and facility size, should enhance regional healthcare accessibility and the quality of cardiac surgery in South Korea.

Synthesis and Characterization of Zinc Phosphate Cement Powder and Cement-forming Liquid

Park, Choon-Keun The Korean Ceramic Society 1997 The Korean journal of ceramics Vol.3 No.4

Chemical composition of cement powder influences the setting time and early compressive strength development. The setting time increases as the amounts of zinc oxide and magnesium oxide are increased. For one day compressive strength development, a cement powder with a composition 90% ZnO, 8% MgO and 2% silica resulted in the highest strength (greater than 1, 090 kg/$\textrm{cm}^2$). Cement-forming liquids also need to be buffered, with both aluminum and zinc ions, for a good consistency and a higher strength of the zinc phosphate cement. These liquids control the setting reactions.

hCG-induced endoplasmic reticulum stress triggers apoptosis and reduces steroidogenic enzyme expression through activating transcription factor 6 in Leydig cells of the testis

Park, Sun-Ji,Kim, Tae-Shin,Park, Choon-Keun,Lee, Sang-Hee,Kim, Jin-Man,Lee, Kyu-Sun,Lee, In-kyu,Park, Jeen-Woo,Lawson, Mark A,Lee, Dong-Seok Society for Endocrinology 2013 Journal of molecular endocrinology Vol.50 No.2

<P>Endoplasmic reticulum (ER) stress generally occurs in secretory cell types. It has been reported that Leydig cells, which produce testosterone in response to human chorionic gonadotropin (hCG), express key steroidogenic enzymes for the regulation of testosterone synthesis. In this study, we analyzed whether hCG induces ER stress via three unfolded protein response (UPR) pathways in mouse Leydig tumor (mLTC-1) cells and the testis. Treatment with hCG induced ER stress in mLTC-1 cells via the ATF6, IRE1a/XBP1, and eIF2α/GADD34/ATF4 UPR pathways, and transient expression of 50 kDa protein activating transcription factor 6 (p50ATF6) reduced the expression level of steroidogenic 3β-hydroxysteroid dehydrogenase △5-△4-isomerase (3β-HSD) enzyme. In an <I>in vivo</I> model, high-level hCG treatment induced expression of p50ATF6 while that of steroidogenic enzymes, especially 3β-HSD, 17α-hydroxylase/C17–20 lyase (CYP17), and 17β-hydrozysteroid dehydrogenase (17β-HSD), was reduced. Expression levels of steroidogenic enzymes were restored by the ER stress inhibitor tauroursodeoxycholic acid (TUDCA). Furthermore, lentivirus-mediated transient expression of p50ATF6 reduced the expression level of 3β-HSD in the testis. Protein expression levels of phospho-JNK, CHOP, and cleaved caspases-12 and -3 as markers of ER stress-mediated apoptosis markedly increased in response to high-level hCG treatment in mLTC-1 cells and the testis. Based on transmission electron microscopy and H&E staining of the testis, it was shown that abnormal ER morphology and destruction of testicular histology induced by high-level hCG treatment were reversed by the addition of TUDCA. These findings suggest that hCG-induced ER stress plays important roles in steroidogenic enzyme expression via modulation of the ATF6 pathway as well as ER stress-mediated apoptosis in Leydig cells.</P>

Effects of Endoplasmic Reticulum Stress Inhibitor Treatment during the Micromanipulation of Somatic Cell Nuclear Transfer in Porcine Oocytes

Park, Yeo-Reum,Park, Hye-Bin,Kim, Mi-Jeong,Jung, Bae-Dong,Lee, Seunghyung,Park, Choon-Keun,Cheong, Hee-Tae The Korean Society of Developmental Biology 2019 발생과 생식 Vol.23 No.1

We examined the effects of endoplasmic reticulum (ER) stress inhibitor treatment during the micromanipulation of porcine somatic cell nuclear transfer (SCNT) on the in vitro development of SCNT embryos. ER stress inhibitors such as salubrinal (200 nM) and tauroursodeoxycholic acid (TUDCA; $100{\mu}M$) were added to the micromanipulation medium and holding medium. The expression of X-box binding protein 1 (Xbp1), ER-stress-associated genes, and apoptotic genes in SCNT embryos was confirmed at the one-cell and blastocyst stages. Levels of Xbp1 splicing and expression of ER-stress-associated genes in SCNT embryos at the one-cell stage decreased significantly with TUDCA treatment (p<0.05). The expression of ER-stress-associated genes also decreased slightly with the addition of both salubrinal and TUDCA (Sal+TUD). The expression levels of caspase-3 and Bcl2-associated X protein (Bax) mRNA were also significantly lower in the TUDCA and Sal+TUD treatments (p<0.05). At the blastocyst stage, there were no differences in levels of Xbp1 splicing, and transcription of ER-stress-associated genes and apoptosis genes between control and treatment groups. However, the blastocyst formation rate (20.2%) and mean blastocyst cell number ($63.0{\pm}7.2$) were significantly higher (p<0.05) for embryos in the TUDCA treatment compared with those for control (12.6% and $41.7{\pm}3.1$, respectively). These results indicate that the addition of ER-stress inhibitors, especially TUDCA, during micromanipulation can inhibit cellular damage and enhance in vitro development of SCNT embryos by reducing stress levels in the ER.