골육종조직 NUCLEASE의 활성조절에 관한 연구

고재경,김용석,강춘식,현병철,오철 한양대학교 의과대학 1996 한양의대 학술지 Vol.16 No.2

Acid DNase and neutral RNase were enzymes known to be associated with processes involved in carcinogenesis and suppression of cancer. These two enzymes were isolated and partially purified in the osteosarcom tissue of bone to find out whether the enzymes isolated were specific to the sarcoma. Also studied were inhibitory actions of protein inhibitors and polynucleotides onacid DNase and neutral RNase to evaluate how these enzymes were regulated in the sarcoma tissue. Activities of acid DNase, neutral RNase and RNase inhibitor were markedly increased and the positive rate of each of the enzymes and inhibitor as a marker for osteosarcoma was high, suggesting the acid DNase, the RNase and the inhibitor could be used as a biochemical marker for the osteosarcoma. Chromatographical analyses revealed that the acid DNase of osteosarcoma tissue was separated in a single peak which was greater than that isolated in control tissue of bone. The neutral RNase in osteosarcoma tissue was separated into 5 isozymes, of which two isozymes were specific to the sarcoma, the other two isozymes were greater in activity (activated) and the rest of two isozyme isolated in the control tissue of bone was disappeared. RNase inhibitor activity was detected in all of the five isozymes isolated fromthe osteosarcoma tissue. The ratio of inhibitor/RNase in the isozyme I fraction was the least of the five isozyme fractions isolated from the sarcoma tissue, indicating that the degree of increment in the inhibitor activity was lesser in the RNase isozyme fraction 1. The acid DNase and RNase isozyme I isolated from the osteosarcoma tissue was inhibited by nucleic acids and polynucleotides, the degree of inhibition being changed with base sequence of the polynucleotides studied. The present study was shown that (1) the acid DNase activity known to be associated with carcinogenesis was greatly increased, (2) the RNase isozyme I activity suggested to be related with suppression of cancer was also greatly increased, (3) the inhibitor activity linked with the isozyme I was decreased relatively and that (4) these enzyme activities activities were inhibited by nucleic acids and polynucleotides studied. The results indicated that acid DNase and RNase isozyme I were associated with carcinogenesis and suppression of osteosarcoma, these actions being regulated not only by protein inhibitors, but also by sequence of polynucleotides.

실고기목 어류 (Syngnathiformes)의 분자계통학적 분류

고범석 ( Beom Seok Koh ),송춘복 ( Choon Bok Song ) 한국수산과학회 2004 한국수산과학회지 Vol.37 No.5

N/A The previous morphology-based taxonomic frameworks within the family Syngnathidae had emphasized the significance of the male brood pouch and reproductive biology in defining the group. However, several different hypotheses had been proposed by different investigators. This study has been carried out to determine the phylogenetic relationships among 19 species belonging to the order Syngnathiformes with three Gasterosteiformes species as outgroup taxa by using the mitochondrial cytochrome b DNA sequences. Phylogenetic analyses based on neighbor-joining distance, maximum parsimony, minimum evolution and maximum likelihood method strongly supported that the family Syngnathidae, the suborder Syngnathoidei and the order Syngnathiformes were all monophyletic group. Although much of previous morphological analyses were supported by our molecular data, there were some significant discrepancies between molecular and morphological work. Such an interesting result was that the weedy seadragon (Phvllopteryx taeniolatus) strongly grouped together with the New Zealand pot-belly seahorse (Hippocampus abdominalis). Considering the markedly different brooding structure between them, this unexpected result might be explained whether by multiple independent origins of brooding structure or by hybridization between the female Hippocampus and other syngnathid species having individual membranous egg compartment. In addition, the suborder Aulostomoidei was paraphyletic group because the shrimpfish (Aeliscus strigatus), belonging to the family Centriscidae, always grouped together with the family Syngnathidae as a sister taxon.

청년기 한국인 얼굴에 대한 계측적 연구 Ⅱ

백두진,안동춘,고기석 대한췌담도연구회 2000 대한췌담도연구회지 Vol.5 No.4

논문 : 지구물리 탐사에 의해 발견된 제주도 용암동굴의 특징과 의미

전용문 ( Yong Mun Jeon ),기진석 ( Jin Seok Ki ),고수연 ( Su Yeon Koh ),김련 ( Lyoun Kim ),류춘길 ( Choon Kil Ryu ) 대한지질공학회 2015 지질공학 Vol.25 No.4

제주도에서 기존에 알려진 동굴 이외에 새로운 지하동굴의 존재를 확인하기 위해 제주시 구좌읍 일대에서 지구물리탐사(전기비저항탐사, GPR탐사, ZHF탐사)를 실시하였다. 탐사결과 여러 지점에서 동굴로 추정되는 이상대가 발견되었고, 이상대의 위치와 규모가 일치하는 27개 지점을 선별하여 시추조사를 실시하였다. 그러나 예상과 달리 이상대의 대부분은 용암의 상하부에 형성된 클링커층이거나 용암 사이에 협재된 고토양층이었으며, 5곳에서만 동굴이 발견되었다. 클링커층과 고토양층은 용암과 다른 암석의 물리적 특징으로 인해 이상체로 반응한 것으로 해석된다. 한편 동굴이 확인된 5개 지점 중 2공은 기존에 알려진 용천동굴을 관통하였고, 3공에서 새로운 동굴이 확인되었다. 용천동굴을 관통한 시추공은 시추당시 동굴의 존재가 확인되지 않았던 구간이었으나 용천동굴이 관통됨에 따라 기존 측량도의 오류를 수정하였다. 한편 시추조사로 새롭게 발견된 석회장식 용암동굴은 길이가 약 180 m이며, 상류방향으로 당처물동굴(약 110 m)과 연결되어 있다. 또한 동굴내부에는 용암곡석, 용암제방, 밧줄구조 등이 발달해 있으며, 탄산염 동굴생성물로는 종유관, 종유석, 석순, 석주, 동굴산호 등이 잘 보존되어 있다. 특히 동굴에서 발견되는 탄산염 동굴생성물은 식물 뿌리를 따라 유입된 지표수에 의해 성장하여 독특한 형태를 띠게 된 것으로 추정된다. Geophysical exploration using electric resistivity, ground penetrating radar (GPR), and impedance high-frequency (ZHF) surveys was conducted in Gujwa-eup, Jeju City, Jeju Island, an island in the Korea Strait, to confirm the existence of new caves near known caves. The exploration revealed a number of anomaly zones, presumed to be caves; 27 sites at suitable locations and depth ranges were selected for drilling and further surveys. However, contrary to predictions, most of the anomaly zones were clinker layers or paleosols intercalated with lavas. Only five boreholes intersected caves. The clinker layers and paleosols were possibly detected as anomalies owing to their different physical properties from the other rocks. Two of the five cave-finding boreholes penetrated Yongcheon Cave; a new cave was found at the other. The two boreholes that penetrated Yongcheon Cave were drilled in areas where the cave has not been previously reported, and thus helped correct an error in the cave distribution map. The cave newly discovered in this boring exploration is 180 m long, and it is connected to the upstream part of Dangcheomul Cave (110 m). The cave contains well-developed lava helictites, lava levees, and ropy structures; carbonate speleothems such as soda straws, stalagmites, columns, and curtain shawls are also well preserved. Notably, the unique shape of the carbonate speleothemsis attributed to their growth in relation to the cavern water that flowed into the cave along plant roots.

청년기 한국인 얼굴에 대한 계측적 연구 Ⅱ

백두진(Doo-Jin Paik),안동춘(Dong-Choon Ahn),고기석(Ki-Seok Koh) 대한체질인류학회 2000 대한체질인류학회지 Vol.13 No.4

본 연구는 한국성인의 체질인류학적 특징을 밝히기 위하여 실시하였다. 18세에서 30세 미만의 남녀대학생 726명 (남자 340명, 여자 386명, 평균나이 21세)을 대상으로 16개 항목을 계측하고 29개 항목의 얼굴과 머리의 지수값을 구하였으며 그 분포를 확인하여 다음과 같은 한국인의 특성을 알 수 있었다. 한국인의 얼굴과 머리에서는 짧은 머리, 높은 머리, 넓고 높은 옆머리, 좁은 얼굴, 좁은 위얼굴, 넓은 턱, 좁은 코, 얇은 입과 넓은 안쪽눈구석사이거리가 특징으로 나타났다. We carried out this study to examine the anthropological characteristics of Korean youths. The subject of this study was 726 college studants(340 male and 386 female) from 18 to 30 years old (average 21 years old). They were measured 16 craniometric items and taken 29 idices of head and face. The characteristics of Korean youths were brachycephal, acrocephalic, hypsicephalic, leptoprosop, lepten, micropside, wide jaw, leptorrhin, thin lipped and wide entocanthione breadth.

간십이지장 인대에서 발생한 신경초종 1 예

김주성(Joo Sung Kim),최문석(Moon Seok Choi),이준혁(Joon Hyoek Lee),고광철(Kwang Cheol Koh),백승운(Seung Woon Paik),박유정(Yu Jeong Park),최원혁(Won Hyeok Choe),홍일철(Il Chul Hong),이춘영(Choon Young Lee),이종철(Jong Chul Rhee),최규 대한소화기학회 2002 대한소화기학회지 Vol.39 No.1

Neurilemmomas are neurogenic tumors that arise from schwann cells of nerve roots. They commonly occur in the head and neck, extremity, mediastinum, and retroperitoneum area, but rarely, they occur in the gastrointestinal tract. In the gastrointestinal tract, the most common site is the stomach. They also occur in the small intestine, mesentery, and appendix. Recently, we have experienced a very rare case of neurilemmoma arising from the hepaticoduodenal ligament. We report this case with a review of literature. (Korean J Gastroenterol 2002;39:68-70)

사람 태아 성상세포에서 새로운 유전자의 분리 및 구조 분석

박주영(Joo Young Park),서희석(Hee Seok Seo),최선주(Sun Ju Choi),박현숙(Hyun Sook Park),이경호(Kyoung-Ho Lee),고춘명(Choon-Myung Koh),이성수(Sung Soo Lee) 대한미생물학회 2003 Journal of Bacteriology and Virology Vol.33 No.1

전남 지방의 설사 자돈에서 분리된 병원성 대장균에 관한 연구

김자숙 ( Ja Sook Kim ),박형춘 ( Hyung Choon Park ),정인호 ( In Ho Chung ),오은희 ( Eun Hee Oh ),박석준 ( Seok Jun Park ),고홍범 ( Hong Bum Koh ) 한국가축위생학회 1996 韓國家畜衛生學會誌 Vol.19 No.2

Porcine E. coli infection is a disease caused by Enterotoxin produced by Enterotoxigenic Escherichia coli (ETEC). Enteric colibacillosis has become an economically important disease in pigs as a result of increasing intensification of farrowing management. The present study undertaken to obtain the antibiotic sensitivity and distribution of serogroups and pili producibility test of ETEC from E. coli isolates in Chonnam. The results obtained were as follows. 1. A total of 71 isolates identified as E. coli employing IMViC system from rectal specimens of 54 piglets with diarrhea. 2. In antibiotic sensitivity test, isolates showed high sensitivity to AN, CM, Fox, GM, but resistance to EM, NA TC. 3. The distribution of 25 isolates of serogroups were 0141:K85(11.3%), 08:K87(8.5%), 064:K-(5.6%), 0138:K81(4.2%), 0139:K82(2.8%), 0157:K88ac(1.4%) and 0149:K91(1.4%). 4. MRHA of guinea pig erythrocytes was detected in 8 out of 25OK serotypes and 9 out of 46 unidentified serotypes. MRHA titers of serotypes showed from 64 to 128 in 0141:K85, 2 in 0138:K81 and no titers in 0139:K82. 5. The production of heat labile enterotoxin of ETEC was detect 39 out of 52 isolates showed β-hemolysin, 7 out of 52 isolates showed γ-hemolysin and 6 out of 52 isolates showed γ-hemolysin by GM1 ganglioside ELISA. The distribution of LT toxin were in 12 isolate showed β-hemolysin, 2 isolates showed α-hemolysin and 3 isolates showed γ-hemolysin in 25OK serotypes.

Effect of HBV Reactivation on the Recurrence of Hepatitis B-related Hepatocellular Carcinoma after Curative Resection in Patients with Low Viral Load

Won Sohn,Jong Man Kim,Choon Hyuk Kwon,Jae Won Joh,Jemma Ahn,Ju Yeon Cho,Dong Hyun Sinn,Geum-Youn Gwak,Moon Seok Choi,Joon Hyeok Lee,Kwang Cheol Koh,Seung Woon Paik,Byung Chul Yoo 한국간담췌외과학회 2014 한국간담췌외과학회 학술대회지 Vol.2014 No.4

LT, Others : O-064 ; High prevalence of steatosis among liver donors who had no evidence of fatty liver on ultrasonography justifies pre-operative liver biopsy

( Joon Seong Ahn ),( Dong Hyun Sinn ),( Geum Youn Gwak ),( Jong Man Kim ),( Choon Hyuck Kwon ),( Jae Won Joh ),( Yong Han Paik ),( Moon Seok Choi ),( Joon Hyeok Lee ),( Kwang Cheol Koh ),( Seung Woon 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.1

Background: The degree of steatosis of donated liver is one of decisive factors that determine graft function in recipient and recovery of remnant liver in living donor. Hence, the assessment of hepatic steatosis is a critical element to judge donor compatibility. We evaluated the prevalence and predictors of steatosis among liver donors who had no evidence of fatty liver on screening ultrasonography (US-negative). Methods: Degree of hepatic steatosis was reviewed for 492 US-negative liver donors (age: 30.1 ± 9.9, male: 301 (61.2%)). Clinical and laboratory variables were analyzed to identify predictors for steatosis. Results: The prevalence of severe (≥ 60%), moderate (30 ~ 59%), mild (5 ~ 29%) and no steatosis (<5%) were 0.6% (3/ 492), 10.8% (53/492), 39.6% (195/492) and 49.0% (241/492), respectively. In multivariate analysis, BMI [kg/m2, OR (95% CI): 1.15, p = 0.021] and serum triglyceride level [mg/dl, OR (95% CI): 1.01, p = 0.004] were independent factors associated with steatosis ≥ 30%. The optimal cutoff for steatosis ≥ 30% by receiver operator curve analysis was 23 kg/m2 for BMI and 88 mg/dl for triglyceride. Steatosis ≥ 30% was significantly more prevalent for liver-donors with high BMI (15.2% vs. 9.0% for BMI ≥ 23 vs. < 23 kg/m2, p = 0.034) and with high serum triglyceride level (15.5% vs. 8.7% for triglyceride ≥ 88 vs. < 88 mg/dl, p = 0.021). Based on these two parameters, the prevalence of steatosis ≥ 30% was 6.6%, 13.0% and 28.6% for none, one and both risk factors, respectively. Conclusions: About a half of US-negative liver donors have steatosis ≥ mild degree and one-tenth of them has ≥ moderate degree. USG assessment is not sufficient to exclude donors with significant steatosis especially for those with high BMI and triglyceride level, and thus, pre-operative liver biopsy should be positively considered for them.