The seasonal detection of AcSBV (Apis cerana sacbrood virus) prevalence in Taiwan

Yu-Shin Nai,Chong-Yu Ko,Pei-Shou Hsu,Wen-Shi Tsai,Yue-Wen Chen,Meng-Hao Hsu,I-Hsin Sung 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.1

The epizootic disease caused by Apis cerana sacbrood virus (AcSBV) occurred in Eastern hive bee, A. cerana, since2015 in Taiwan. A large-scale survey of this disease from September and December 2016 in Taiwan was performedincluding symptom check and molecular identification in honey bees of A. cerana hives and several A. mellifera hives, which were co-cultured with A. cerana. Based on the nucleotide sequences of partial VP1, thephylogenetic analysis with those of the known AcSBV isolates revealed that most of AcSBV isolates from Taiwanwere closely relative to SBV-FZ and -JL isolates from China, whereas only one sample (N15-5-1) was in a distinctcluster, which was closely relative to SBV-LN from China too. The AcSBV prevalence was occurring in A. ceranahives in most areas of Taiwan except for those in Hualien and Pingtung Counties in Taiwan. Notably, the AcSBVprevalence rate showed the temporal increase from 47.1% to 69.6% within 4 months. In addition, 37.5% ofAcSBV prevalence rate was found in A. mellifera hives. It showed that A. mellifera was also susceptible to AcSBVinfection. The present results would provide the information on the epidemiology and for prospective research.

DTV 튜너를 위한 CMOS Fractional-N 주파수합성기

고승오(Ko, Seung-O),서희택(Seo, Hee-Teak),박종태(Park, Jong-Tae),유종근(Yu, Chong-Gun) 한국전기전자학회 2010 전기전자학회논문지 Vol.14 No.1

최근 TV 방송의 새로운 시장인 DTV 시장이 넓어지면서 DTV 튜너에 대한 요구도 많아지고 있다. DTV 튜너를 설계하는 데에는 많은 어려운 부분이 있지만, 가장 어려운 부분 중에 하나가 주파수합성기이다. 본 논문에서는 DTV 튜너를 위한 주파수합성기 회로를 CMOS 공정을 사용하여 설계하였다. 설계한 주파수합성기는 DTV(ATSC)의 주파수 대역(54~806MHz)을 만족한다. 하나의 VCO를 사용하여 광대역을 만족시킬 수 있는 구조를 제안하고, LO pulling 효과를 최소화 하기위하여 1.6~3.6GHz 대역에서 동작하도록 설계하였다. 또한 고주파 대역과 저주파 대역에서의 VCO 이득의 차이와 주파수 간격의 변화를 줄여 안정적인 광대역 특성을 구현하였다. 모의실험 결과, 설계한 VCO의 이득은 59~94MHz(17.7MHz/V,23%)이고, 주파수 간격은 26~42.5MHz (8.25MHz/V,24%)이며, tuning range는 76.9%이다. 설계된 주파수합성기의 위상잡음은 100kHz offset에서 -106dBc/Hz이고, 고착시간은 약 정도이다. 설계된 회로는 1.8V 전원전압에서 20~23mA의 전류를 소모하며 칩 면적은 PAD를 포함하여 2.0mm×1.8mm이다. The Digital TV(DTV) standard has ushered in a new era in TV broadcasting and raised a great demand for DTV tuners. There are many challenges in designing a DTV tuner, of which the most difficult part is the frequency synthesizer. This paper presents the design of a frequency synthesizer for DTV Tuners in a CMOS process. It satisfies the DTV(ATSC) frequency band(54~806MHz). A scheme is proposed to cover the full band using only one VCO. The VCO has been designed to operate at 1.6~3.6GHz band such that the LO pulling effect is minimized, and reliable broadband characteristics have been achieved by reducing the variations of VCO gain and frequency step. The simulation results show that the designed VCO has gains of 59~94MHz(17.7MHz/V,23%) and frequency steps of 26~42.5MHz(8.25MHz/V,24%), and a very wide tuning range of 76.9%. The designed frequency synthesizer has a phase noise of -106dBc/Hz at 100kHz offset, and the lock time is less than sec. It consumes 20~23mA from a 1.8V supply, and the chip size including PADs is 2.0mm×1.8mm.

사이토카인 유전자 함유 바이러스 유사입자의 제조

오유경(Yu Kyoung Oh),손태종(Tae Jong Son),신광숙(Kwang Sook Sin),강민정(Min Jeong Kang),김정목(Jung Mogg Kim),김남근(Nam Keun Kim),고정재(Jung Jae Ko),김종국(Chong Kook Kim) 한국약제학회 2001 Journal of Pharmaceutical Investigation Vol.31 No.3

N/A Human papillomavirus (HPV) infection is known to cause cervical cancers. Human papillomavirus-like particles (VLP) have been studied as preventive vaccines of cervical cancers. To develop VLP as a therapeutic gene carrier, we studied the method to encapsulate cytokine genes in virus-like particles. HPV type 16 capsid L1 genes were amplified by polymerase chain reaction and cloned into T vector. L1 gene was then inserted into baculovirus transfer vector. The clone of baculovirus encoding L1 gene was isolated and used to express L1 protein in Sf 21 insect cells. VLP were purified by CsCl density gradient and ultracentrifugation. VLP were disassembled to capsomer units by treatment of a reducing agent. Given that interleukin-2 (IL-2) genes have been used in anticancer gene therapy and as a molecular adjuvant, IL-2 cytokine plasmids were chosen as a model gene. IL-2 plasmids were incubated with the disassembled capsomer suspension. To reassemble the particles, the mixture of capsomers and cytokine plasmids was dialyzed. The disassembly and reassembly of VLP were confirmed by transmission electron microscopy. The entrapment of cytokine plasmids in reassembled VLP was tested by the stability of plasmids against DNase I. After treatment of reassembled virus-like particles with DNase I, discrete IL2 DNA band was observed. Our results indicate that IL-2 cytokine plasmid (3.5 kb size) can be encapsulated in the viruslike particles, suggesting the potential of VLP as a gene delivery system. Moreover, VLP containing the adjuvant cytokine plasmids might function as more effective subunit vaccines.

연구논문 : 생명과학 ; interleukin-2 gene adjuvant를 함유한 human papillomavirus-Like particles의 점막 및 전신 면역 체계의 증강

오유경 ( Yu Kyoung Oh ),손태종 ( Tae Jong Sohn ),박정숙 ( Jeong Sook Park ),강민정 ( Min Jeong Kang ),최한곤 ( Han Gon Choi ),김정애 ( Jung Ae Kim ),김원기 ( Won Ki Kim ),고정재 ( Jung Jae Ko ),김종국 ( Chong Kook Kim ) 영남대학교 약품개발연구소 2005 영남대학교 약품개발연구소 연구업적집 Vol.15 No.-

Hyaluronic acid derivative-based self-assembled nanoparticles for the treatment of melanoma.

Jin, Yu-Jin,Termsarasab, Ubonvan,Ko, Seung-Hak,Shim, Jae-Seong,Chong, Saeho,Chung, Suk-Jae,Shim, Chang-Koo,Cho, Hyun-Jong,Kim, Dae-Duk Kluwer Academic/Plenum Publishers 2012 Pharmaceutical research Vol.29 No.12

<P>Hyaluronic acid-ceramide (HACE)-based nanoparticles (NPs) were developed for the targeted delivery of doxorubicin (DOX), and their antitumor efficacy for melanoma was evaluated.</P>

DTV 튜너를 위한 48MHz~1675MHz 주파수합성기 설계

고승오,서희택,권덕기,유종근,Ko, Seung-O,Seo, Hee-Teak,Kwon, Duck-Ki,Yu, Chong-Gun 한국정보통신학회 2011 한국정보통신학회논문지 Vol.15 No.5

본 논문에서는 DTV 응용을 위한 광대역 주파수 합성기 회로를 $0.18{\mu}m$ CMOS 공정을 사용하여 설계하였다. 설계한 주파수 합성기는 DTV의 모든 주파수 대역을(48MHz~1675MHz) 만족한다. 하나의 VCO만을 사용하여 광대역을 만족시킬 수 있는 구조를 제안하였으며, 고주파 대역과 저주파 대역에서의 VCO 이득의 차이와 주파수 간격의 변화를 줄여 안정적인 광대역 특성을 구현하였다. 모의실험 결과, VCO의 발진주파수 범위는 1.85GHz~4.22GHz이며, 4.2GHz에서 위상잡음은 100kHz offset에서 -89.7dBc/Hz이다. VCO 이득은 62.4~95.8MHz/V(${\pm}21.0%$)이고 주파수 간격은 22.9~47.9MHz(${\pm}35.3%$)이다. 설계된 주파수합성기의 고착시간은 약 $0.15{\mu}s$이다. 제작된 칩을 측정한 결과 VCO는 2.05~3.4GHz의 대역에서 발진하는 것을 확인하였다. 설계된 주파수 보다 shift down 되었지만 마진을 두어서 설계를 하였기 때문에 DTV 튜너로 사용할 수 있는 주파수 대역은 만족한다. 설계된 회로는 1.8V 전원 전압에서 23~27mA의 전류를 소모한다. 칩 면적은 PAD를 포함하여 $2.0mm{\times}1.5mm$이다. In this paper a wideband frequency synthesizer is designed for DTV tuners using a $0.18{\mu}m$ CMOS process. It satisfies the DTV frequency band(48~1675MHz). A scheme is proposed to cover the full band using only one VCO and reliable broadband characteristics are achieved by reducing the variations of VCO gains and frequency steps. The simulation results show that the designed VCO has frequency range of 1.85~4.22GHz, phase noise at 4.22GHz of -89.7dBc/Hz@100kHz, gains of 62.4~95.8MHz/V(${\pm}21.0%$) and frequency steps of 22.9~47.9MHz(${\pm}35.3%$). The designed VCO has a phase noise of -89.75dBc/Hz at 100kHz offset. The designed synthesizer has a lock time less than $0.15{\mu}s$. The measured VCO tuning range is 2.05~3.4GHz. The frequency range is shifted down but still satisfy the target range owing to the design for enough margin. The designed circuit consumes 23~27mA from a 1.8V supply, and the chip size including PADs is $2.0mm{\times}1.5mm$.

Supplementation with extract of <i>Gynostemma pentaphyllum</i> leaves reduces anxiety in healthy subjects with chronic psychological stress: A randomized, double-blind, placebo-controlled clinical trial

Choi, Eun-Kyung,Won, Yu Hui,Kim, Soon-Young,Noh, Soon-Ok,Park, Soo-Hyun,Jung, Su-Jin,Lee, Chong Kil,Hwang, Bang Yeon,Lee, Myung Koo,Ha, Ki-Chan,Baek, Hyang-Im,Kim, Hye-Mi,Ko, Myoung-Hwan,Chae, Soo-Wan Urban und Fischer Verlag 2019 Phytomedicine Vol.52 No.-

<P><B>Abstract</B></P> <P><B>Background</B></P> <P>The ethanol extract of <I>Gynostemma pentaphyllum</I> Makino leaves (EGP) has been reported recently to have anxiolytic effects on chronically stressed mice models.</P> <P><B>Purpose</B></P> <P>We aimed to investigate the efficacy and safety of EGP on anxiety level in healthy Korean subjects under chronic stressful conditions.</P> <P><B>Study design</B></P> <P>Double-blind, placebo-controlled trial.</P> <P><B>Methods</B></P> <P>This study was conducted with 72 healthy adults who had perceived chronic stress and anxiety with a score on the State-Trait Anxiety Inventory (STAI) from 40 to 60. Participants were randomly assigned to receive either EGP (200 mg, twice a day, <I>N</I> = 36) or placebo (<I>N</I> = 36). All participants were exposed to repetitive loads of stress by performing the serial subtraction task for 5 min every second day during the 8-week intervention. Primary outcome of Trait-STAI and secondary outcomes of State-STAI, total score of STAI, Hamilton Anxiety Inventory (HAM-A), Beck Anxiety Inventory (BAI), blood norepinephrine and adrenocorticotropic hormone (ACTH), salivary cortisol and alpha-amylase, cardiovascular autonomic nervous system (ANS) functional test, and heart rate variability (HRV) test were measured before and after intervention.</P> <P><B>Results</B></P> <P>After the 8-week intervention, the EGP significantly lowered the score of the Trait Anxiety Scale of the STAI (T-STAI) by 16.8% compared to the placebo (<I>p</I> = 0.041). The total score on the STAI decreased by 17.8% in the EGP group and tended to improve compared with that of the placebo group (<I>p</I> = 0.067). There were no significant differences in the changes in score of S-STAI, HAM-A, BAI, and other parameters from baseline between the two groups. There was no causal relationship between the ingestion of EGP and adverse drug reactions.</P> <P><B>Conclusion</B></P> <P>We found that supplementation with EGP reduced “anxiety proneness” in subjects under chronic psychological stress, as shown by a decrease in the score of T-STAI and the tendency for decrease in the total score of STAI. This result suggests that EGP supplementation can be used as a regimen to safely reduce stress and anxiety; however, more studies are needed to establish the long-term safety and effectiveness.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Prevalence of Anaplasma and Bartonella spp. in Ticks Collected from Korean Water Deer(Hydropotes inermis argyropus)

Jun-Gu Kang,Sungjin Ko,Heung-Chul Kim,Sung-Tae Chong,Terry A. Klein,Jeong-Byoung Chae,Yong-Sun Jo,Kyoung-Seong Choi,Do-Hyeon Yu,Bae-Keun Park,Jinho Park,Joon-Seok Chae 대한기생충학열대의학회 2016 The Korean Journal of Parasitology Vol.54 No.1

Deer serve as reservoirs of tick-borne pathogens that impact on medical and veterinary health worldwide. In the Republic of Korea, the population of Korean water deer (KWD, Hydropotes inermis argyropus) has greatly increased from 1982 to 2011, in part, as a result of reforestation programs established following the Korean War when much of the land was barren of trees. Eighty seven Haemaphysalis flava, 228 Haemaphysalis longicornis, 8 Ixodes nipponensis, and 40 Ixodes persulcatus (21 larvae, 114 nymphs, and 228 adults) were collected from 27 out of 70 KWD. A total of 89/363 ticks (266 pools, 24.5% minimum infection rate) and 5 (1.4%) fed ticks were positive for Anaplasma phagocytophilum using nested PCR targeting the 16S rRNA and groEL genes, respectively. The 16S rRNA gene fragment sequences of 88/89 (98.9%) of positive samples for A. phagocytophilum corresponded to previously described gene sequences from KWD spleen tissues. The 16S rRNA gene fragment sequences of 20/363 (5.5%) of the ticks were positive for A. bovis and were identical to previously reported sequences. Using the ITS specific nested PCR, 11/363 (3.0%) of the ticks were positive for Bartonella spp. This is the first report of Anaplasma and Bartonella spp. detected in ticks collected from KWD, suggesting that ticks are vectors of Anaplasma and Bartonella spp. between reservoir hosts in natural surroundings.

Quarter-Rate Bang-Bang 위상검출기를 사용한 0.18㎛ CMOS 10Gbps CDR 회로 설계

차충현(Cha, Chung-Hyeon),고승오(Ko, Seung-O),서희택(Seo, Hee-Taek),박종태(Park, Jong-Tae),유종근(Yu, Chong-Gun) 한국전기전자학회 2009 전기전자학회논문지 Vol.13 No.2

통신시스템에서 데이터 전송이 고속으로 이루어지면서, 하드웨어의 복잡성, 전력소모, 가격 등의 이유로 클럭을 제외한 데이터만 수신단으로 보내는 방식이 사용되어지고 있다. 따라서, 고속으로 수신된 데이터에서 클럭 신호를 추출하는 것이 필요하며, 추출된 클럭을 이용하여 데이터를 복원하는 클럭/데이터 복원회로(CDR)에 관한 연구가 활발히 이루어지고 있다. 본 논문에서는 0.18um CMOS 공정을 이용하여 10Gbps CDR 회로를 설계하였다. 전력소모와 회로의 복잡도를 줄이기 위해 quarter-rate bang-bang 유형의 위상 검출기를 사용하였으며, 지터 특성 향상을 위해 LC 유형의 4단 VCO를 사용하였다. 모의실험 결과, 설계된 CDR 회로는 1.8V 전원전압에서 80mW의 전력을 소모하며, 2.2ps,pp의 클럭 지터 특성을 보인다. 패드를 제외한 칩 레이아웃 면적은 1.26mm×1.05mm이다. With recent advancement of high-speed, multi-gigabit data transmission capabilities, transmitters usually send data without clock signals for reduction of hardware complexity, power consumption, and cost. Therefore clock and data recovery circuits(CDR) become important to recover the clock and data signals and have been widely studied. This paper presents the design of 10Gbps CDR in 0.18m CMOS process. A quarter-rate bang-bang phase detector is designed to reduce the power and circuit complexity, and a 4-stage LC-type VCO is used to improve the jitter characteristics. Simulation results show that the designed CDR consumes 80mW from a 1.8V supply, and exhibits a peak-to-peak jitter of 2.2ps in the recovered clock. The chip layout area excluding pads is 1.26mm×1.05mm.

DC 유형의 에너지 자원을 활용한 저전력 에너지 하베스팅 시스템

이형선(Hyeong-Sun Lee),고태경(Tae-Kyung Ko),임현(Hyun Im),유종근(Chong-Gun Yu) 한국정보통신학회 2019 한국정보통신학회 종합학술대회 논문집 Vol.23 No.2

본 논문에서는 DC 유형의 에너지 자원을 활용한 저전력 에너지 하베스팅 시스템 회로에 대해 기술한다. 설계된 회로는 열전소자나 광전소자로부터 수확된 에너지를 변환하여 저장 커패시터에 저장한다. 제안된 회로는 크게 MPPT 인터페이스 회로와 DC-DC 변환기로 구성된다. MPPT 인터페이스 회로는 에너지 유형(빛, 열)에 따라 MPPT(Maximum Power Point Tracking) 동작을 통해 최대 전력을 수확한다. DC-DC 변환기는 MPPT 인터페이스를 통해 수확되는 에너지를 Buck-Boost 하여 커패시터에 저장한다. 제안된 회로는 0.35㎛ CMOS 공정으로 설계하였고, 설계된 칩 면적은 1531㎛ x 1212㎛ 이다. This paper describes a low-power energy harvesting system for DC-type energy sources. The designed circuit converts the energy harvested from thermoelectric or photovoltaic devices, and then, store it into a storage capacitor. The proposed circuit consists of an MPPT interface circuit and a DC-DC buck-boost converter. The MPPT interface circuit harvests maximum available power from input sources through MPPT(Maximum Power Point Tracking) operation according to the energy type (thermoelectric, photovoltaic). The DC-DC converter performs boost or buck conversion with the energy harvested through the MPPT interface, and store the converted energy into a storage capacitor. The proposed circuit is designed with 0.35㎛ CMOS process, and the designed chip area is 1531㎛ x 1212㎛.