Radiosynthesis and <i>in vitro</i> evaluation of 1‐(tetrahydro‐5‐hydroxy‐6‐(hydroxymethyl)‐2H‐pyran‐3‐yl)‐5‐[¹²⁵I]iodouracil: A new potential agent for HSV1‐tk

Jo, Nam Hyun,Kim, Jung Young,El‐,Gamal, Mohammed I.,Choi, Won‐,Kyoung,Park, Jin‐,Hun,Kim, Eun Jung,Cho, Jung‐,Hyuck,Ha, Hyun‐,Joon,Choi, Tae Hyun,Oh, Chang‐,Hyun John Wiley Sons, Ltd. 2011 Journal of labelled compounds & radiopharmaceutica Vol.54 No.2

<P><B>Abstract</B></P><P>Synthesis, radiolabelling, and <I>in vitro</I> evaluation of a new <SUP>125</SUP>I‐labelled iodouracil hexitol nucleoside analogue are reported. The target compound was successfully synthesized by an iodination–destannylation method and then purified by reverse phase HPLC. The radiochemical purity of the product was >99% with decay‐corrected yields of 48±3%. <I>In vitro</I> cellular uptake testing was carried out using MCA and MCA‐tk cell lines for comparison of compound 1 with [<SUP>18</SUP>F]FHBG. The newly synthesized compound 1 showed higher accumulation in herpex simplex virus type 1 thymidine kinase (HSV1‐tk) gene expression cell line (MCA‐tk cell line) than in the wild type MCA cell line compared with [<SUP>18</SUP>F]FHBG. The MCA‐tk to MCA cellular uptake ratio for compound 1 was higher than that of [<SUP>18</SUP>F]FHBG from 2 h after incubation. The radioiodine‐labelled compound 1 (I‐125, <I>t</I><SUB>1/2</SUB>=59.37 days) has a longer physical half‐life than F‐18‐(<I>t</I><SUB>1/2</SUB>=110 min) labelled FHBG. Radioiodine‐labelled compound 1 could be used for monitoring gene expression for a long time. The selectivity for MCA‐tk cell line makes compound 1 a promising imaging agent for HSV1‐tk expression. Copyright © 2010 John Wiley & Sons, Ltd.</P>

일별 국지기온 결정에 미치는 관측지점 표고영향의 계절변동

윤진일(Jin I Yun),최재연(Jae Yeon Choi),안재훈(Jae Hoon Ahn) 한국농림기상학회 2001 한국농림기상학회지 Vol.3 No.2

Usage of ecosystem models has been extended to landscape scales for understanding the effects of environmental factors on natural and agro-ecosystems and for serving as their management decision tools. Accurate prediction of spatial variation in daily temperature is required for most ecosystem models to be applied to landscape scales. There are relatively few empirical evaluations of landscape-scale temperature prediction techniques in mountainous terrain such as Korean Peninsula. We derived a periodic function of seasonal lapse rate fluctuation from analysis of elevation effects on daily temperatures. Observed daily maximum and minimum temperature data at 63 standard stations in 1999 were regressed to the latitude, longitude, distance from the nearest coastline and altitude of the stations, and the optimum models with r^2 of 0.65 and above were selected. Partial regression coefficients for the altitude variable were plotted against day of year, and a numerical formula was determined for simulating the seasonal trend of daily lapse rate, i.e., partial regression coefficients. The formula in conjunction with an inverse distance weighted interpolation scheme was applied to predict daily temperatures at 267 sites, where observation data are available, on randomly selected dates for winter, spring and summer in 2000. The estimation errors were smaller and more consistent than the inverse distance weighting plus mean annual lapse rate scheme. We conclude that this method is simple and accurate enough to be used as an operational temperature interpolation scheme at landscape scale in Korea and should be applicable to elsewhere.

Structural and functional characterization of <i>Caenorhabditis elegans</i> α-catenin reveals constitutive binding to β-catenin and F-actin

Kang, Hyunook,Bang, Injin,Jin, Kyeong Sik,Lee, Boyun,Lee, Junho,Shao, Xiangqiang,Heier, Jonathon A.,Kwiatkowski, Adam V.,Nelson, W. James,Hardin, Jeff,Weis, William I.,Choi, Hee-Jung American Society for Biochemistry and Molecular Bi 2017 The Journal of biological chemistry Vol.292 No.17

<P>Intercellular epithelial junctions formed by classical cadherins, beta-catenin, and the actin-binding protein alpha-catenin link the actin cytoskeletons of adjacent cells into a structural continuum. These assemblies transmit forces through the tissue and respond to intracellular and extracellular signals. However, the mechanisms of junctional assembly and regulation are poorly understood. Studies of cadherin-catenin assembly in a number of metazoans have revealed both similarities and unexpected differences in the biochemical properties of the cadherin center dot catenin complex that likely reflect the developmental and environmental requirements of different tissues and organisms. Here, we report the structural and biochemical characterization of HMP-1, the Caenorhabditis elegans alpha-catenin homolog, and compare it with mammalian alpha-catenin. HMP-1 shares overall similarity in structure and actin-binding properties, but displayed differences in conformational flexibility and allosteric regulation from mammalian alpha-catenin. HMP-1 bound filamentous actin with an affinity in the single micromolar range, even when complexed with the beta-catenin homolog HMP-2 or when present in a complex of HMP-2 and the cadherin homolog HMR-1, indicating that HMP-1 binding to F-actin is not allosterically regulated by the HMP-2.HMR-1 complex. The middle (i.e. M) domain of HMP-1 appeared to be less conformationally flexible than mammalian alpha-catenin, which may underlie the dampened effect of HMP-2 binding on HMP-1 actin-binding activity compared with that of the mammalian homolog. In conclusion, our data indicate that HMP-1 constitutively binds beta-catenin and F-actin, and although the overall structure and function of HMP-1 and related alpha-catenins are similar, the vertebrate proteins appear to be under more complex conformational regulation.</P>

개회로 CO_2/H_2O 적외선 기체 분석기 보정 인자의 변동성과 장기 플럭스 관측에 미치는 영향

최태진,윤진일,임종환,박은우,김준 한국농림기상학회 2002 한국농림기상학회지 Vol.4 No.2

Calibration experiments were executed to document pertinent calibration methods for open-path infrared gas analyzer (OP-2) in field operations and to quantify their performance characteristics in continuous long-term flux measurements. Based on our results, we concluded: (1) flow rate of 2.0 L min^-1 can be used for calibration instead of the recommended 0.5 L min^-1. Such faster flow rate brings the sampled air in the calibration hood at equilibrium within 5 min for CO_2 and 10 min for H_2O; (2) after reaching equilibrium, two-minute average sampling for related variables per each concentration may be sufficient; (3) use of four concentration is needed to derive the nonlinear calibration equation for water vapor with 1% uncertainty of flux measurement; and (4) the resultant calibration interval for OP-2 for both CO_2 and H_2O is approximately one month.

고성능 다공 슬레트 핀 M/C Form Roll의 Roll Stand부 마찰 특성

최원식,이삼연,권주리,진은영,문희준,김원구,이인 한국공작기계학회 2009 한국공작기계학회 춘계학술대회논문집 Vol.2009 No.-

This study investigates the friction of the roll stands in the high performance multi- holes slate fin machine which pin is using in heat transfer of radiator, oil cooler, inter cooler, condenser and evaporator. The roll stand part is very important to maintain the high performance fin machine. This multi-hates form roll type is the first time in our country so it wilt be helpful to increases the export and product of heat transfer. It was include the technical of form rolling system which was self development. Then it wilt be improve the electric home appliances, future motor vehicle device and industrial machine.

2-Chlorodeoxyadenosine치료로 완전관해된 모발상세포 백혈병 1례

신진호,김연수,이영열,최일영,김인순 大韓血液學會 1996 大韓血液學會誌 Vol.32 No.3

2-Chlorodeoxyadenosine치료로 완전관해된 모발상세포 백혈병 1례

신진호,김연수,이영열,최일영,김인순 大韓血液學會 1996 大韓血液學會誌 Vol.31 No.2

도시효과를 고려한 일 최저기온의 월별 평년값 분포 추정

최재연 ( Jae Yeon Choi ),윤진일 ( Jin I. Yun ) 한국농림기상학회 2002 한국농림기상학회지 Vol.4 No.4

This study was conducted to remove the urban heat island effects embedded in the interpolated surfaces of daily minimum temperature in the Korean Peninsula. Fifty six standard weather stations are usually used to generate the gridded temperature surface in South Korea. Since most of the weather stations are located in heavily populated and urbanized areas, the observed minimum temperature data are contaminated with the so-called urban heat island effect. Without an appropriate correction, temperature estimates over rural area or forests might deviate significantly from the actual values. We simulated the spatial pattern of population distribution within any single population reporting district (city or county) by allocating the reported population to the urban pixels of a land cover map with a 30 by 30 m spacing. By using this digital population model (DPM), we can simulate the horizontal diffusion of urban effect, which is not possible with the spatially discontinuous nature of the population statistics for each city or county. The temperature estimation error from the existing interpolation scheme, which considers both the distance and the altitude effects, was regressed to the DPMs smoothed at 5 different scales, i.e., the radial extent of 0.5, 1.5, 2.5, 3.5 and 5.0 km. Optimum regression models were used in conjunction with the distance-altitude interpolation to predict monthly normals of daily minimum temperature in South Korea for 1971-2000 period. Cross validation showed around 50% reduction in terms of RMSE and MAE over all months compared with those by the conventional method.

Therapeutic targeting of subdural medulloblastomas using human neural stem cells expressing carboxylesterase

Lim, S-H,Choi, S A,Lee, J Y,Wang, K-C,Phi, J H,Lee, D-H,Song, S H,Song, J H,Jin, X,Kim, H,Lee, H J,Lim, I,Kim, S U,Kim, S-K Nature America, Inc. 2011 Cancer gene therapy Vol.18 No.11

The prognosis of medulloblastoma has improved significantly because of advances in multi-modal treatments; however, metastasis remains one of the prognostic factors for a poor outcome and is usually associated with tumor recurrence. We evaluated the migratory potential and therapeutic efficacy of genetically engineered human neural stem cells (NSCs) that encode a prodrug enzyme in the subdural medulloblastoma model. We genetically modified HB1.F3 (F3) immortalized human NSCs to express rabbit carboxylesterase (rCE) enzyme, which efficiently converts the prodrug CPT-11 (Irinotecan) into an active anti-cancer agent (SN-38). To simulate clinical metastatic medulloblastomas, we implanted human medulloblastoma cells into the subdural spaces of nude mice. rCE expressing NSCs (F3.rCE) were labeled with fluorescence magnetic nanoparticle for in vivo imaging. The therapeutic potential of F3.rCE was confirmed using a mouse subdural medulloblastoma model. The majority of intravenously (i.v.) injected, F3.rCE cells migrated to the subdural medulloblastoma site and a small number of F3.rCE cells were found in the lungs, pancreas, kidney and liver. Animals that received F3.rCE cells in combination with prodrug CPT-11 survived significantly longer (median survival: 142 days) than control mice that received F3.rCE cells only (median survival: 80 days, P<0.001) or CPT-11 only (median survival: 118 days, P<0.001). In conclusion, i.v. injected F3.rCE NSCs were able to target subdural medulloblastomas and demonstrate therapeutic efficacy. Our study provides data that supports further investigation of stem-cell-based gene therapy against metastatic medulloblastomas.

대학생의 인터넷중독 및 스마트폰 중독 정도와 미술 치료 인식에 대한 조사 연구

박혜원,송승윤,윤하영,이경현,이소영,이지원,진예은,최시온,허은서,황다빈,신주현,이인영 이화여자대학교 간호학회 2018 이화간호학회지 Vol.- No.52

Purpose: Investigate the level of Internet and smart phone addiction of college students and difference of their perception on the art therapy. Method: Data was collected using 4 categories of questionnaires. Participants of this study were 383 college students who are currently attending universities located in seoul, Kyung-Ki and Incheon. The Chi-square test, One-way Analysis of Variance, Scheffé test were performed using IBM SPSS Statistics 23.0 Result: First, the study has established that the status of attending universities, grade, people who living with, age affected the level of Internet addiction of college students. In terms of the level of smart phone addiction of college students, the status of attending universities, gender, age were the affective factors. Second, there was a significant difference on the perception of the advantages of the art therapy and the level of acknowledging it, depending on the level of Internet addiction. Finally, depending on the level of smartphone addiction, there was a significant difference in the level of perception of the art therapy, expectation toward the art therapy and the helpfulness of art therapy. The more the participants are close to the addicted level, the more they want to experience the art therapy. Conclusion: These results suggest. First, it is necessary to use bigger group of participants. Second, it is necessary to improve the research methods for college students. Third, nurse should offer holistic care toward the patients regarding their general characteristics by adapting this study. Finally, it is necessary to improve the art therapy programs for the college students who are addicted to the Internet and smartphone and to develop researches proving the effectiveness of these programs.