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정상임신의 태반 Alkaline Phosphatase활성에 관한 임상적 연구
최병관(BK Choi),이재현(JH Lee) 대한산부인과학회 1985 Obstetrics & Gynecology Science Vol.28 No.8
In order to determine the subcellular localization of alkaline phosphatase in human term placenta twenty-two cases of normal term placenta were preared by differen tial centrifugal method There were devided to whole homogenate uncleic fraction mitochondrial fraction microsomal fraction adn supernatant fraction And the specifc activity of alkaline phosphatase in each fractions was measured The results were as following: 1. The enzymatic activity of alkaline phosphatase in whole homogenate of term placenta was 429.23±105.71 K-A unit (King-Amstrong unit/mg of protein/30 minutes), and it was statistically signifcant(P<0.001) 2. The activity of alkaline phosphatase in nucleic fraction was 215,36±64.67 K-A unit (4.6%) 3. The activity of alkaline phosphatase in mitochondrial fraction was 2,056.10±699.49 K-A unit(44%) 4.The activity of alkaline phosphatase in microsomal fraction was 2,237.78±706.18 K-A unit(48%) 5. The activity of alkaline phosphatase in supernatant fraction was 151.46±32.27 K-A unit(3.3%) 6.The correlations of the specifc activity of alkaline phosphatase between whole homogenate and each fractions were statistically signkfcant(p<0.001)
이봉구(BK Lee),최인준(IJ Choi),이유복(YB Lee),김동식(DS Kim) 대한산부인과학회 1975 Obstetrics & Gynecology Science Vol.18 No.7
자궁경부 및 질도말표본과 자궁경부생검을 병행한 총 88례를 분석하여 다음과 같은 결론을 얻었다. 1. 총 88례의 질환별 분포는 만성자궁경부염 34예, 경도, 중증도 및 고도의 상피이상증식증 각각 3예가 있었으며 상피내암 22례 및 침습암 33예가 있었고 침습암중 2례는 현미경적 침 습암이었다. 2. 상피이상증식증의 도말표본 특징은 비정상세포들이 거의 모두가 다각형이며 유리된 배열 소견을 볼수 있었고, 심한 염증 및 출혈의 배경소견은 많지 않았다. 3. 상피내암의 도말표본 특징은 비정상 세포들은 대부분 원형내지 타원형이었으며 유리된 배열이 많이 나타났으나 다수의 유합배열도 관찰되엇고 핵은 비정상세포의 67%가 균일한 굵은 과립상의 염색질 소견을 보였다. 4. 침습암의 도말표본 특징은 비정상세포들의 모양이 원형내지 타원형이 가장 많이 나타나 나 불규칙한 모양의 세포도 자주 볼수 있었고 유리 및 융합된 세포 배열을 관찰하였으며 심 한 염증 및 출혈소견을 지닌 배경이 많았다. 5. 상피내임을 소형 및 대형 세포암, 침습암을 비각화대형세포암, 각화세포암 및 소형세포암 으로 분류 검색한 결과 소형 및 대형 상피내암은 각각 2례, 및 20례가 잇었으며 비각화대형 및 각화세포침습암은 각각 71례 및 6례가 있었고 세포학적 검사로 15례 및 2례에서 검색이 가능하였다. 6. 세포학적 진단 및 조직학적 진단과의 일치율은 중등도 및 고도의 상피이상증식증 각 3례 (100%) 상피내암 22례중 21예(95%) 침습암 23례중 현미경학적 침습암 2례와 불량한 도말표 본 1례를 제외하면 20례(100%)이었으며 총체적으로는 51례중 47례로서 92%의 일치율을 보 였다. 이상의 결과를 종합분석하면 세포병리학적검사로 자궁경부병변 즉 상피이상증식증, 상피내 암 및 침습암 등을 진단하는데에도 정확율이 높으며 자궁경부암의 예후와 밀접한 관련이 있 다고 알려진 유형까지도 구별이 가능함으로 자궁경부암의 예방 조기진단 및 치료에 큰 도움 이 될 것으로 사료된다. 1. The cervical lesions of 88 cases consisted of 34 cases of chronic cervicitis, 9 cases of epithelial dysplasia 22 cases of carcinoma in situ and 23 cases of invasive carcinoma including 2 cases of microinvasion. 2. The characteistic of the smear in epithelial dysplasia were polyhedarl in shape, isolated in arrangement and uniformly finely granular in chromatin pattern (96%) without nucleoli. 3. The characteristics of the smear of carcinoma in situ were abnormal cells with round ot oval in shape and either isolated or syncytial in all arrangements . The abnormal cells of the smear had the nuclei with uniformly coarse chromatin in 67 percent of cells. 4. The characteristics of the invasive carcinoma in smear were round oval in shape, equal degrees of either isolated or syncytial in arraggements with frequent inflamatory and bloody background. The 55 percent of abnormal cells showed irreqularly coarse granular chromatine of nuclei with 6% of micronueleoli and 14 % of marconucleioli. 5. The 22 cases of carcinoma in situ consisted of 2 cases of small cell type and 20 cases of large cell type. The cytologic diagnosis was correct in 15 cases out of 17 cases of non-keratinizing large cell type and 2 cases out of 6 cases of keratinizing cell type. 6. The ratio of positive correlation between cytologic and histopathologic diagnoses was 100% in moderate to severe degree of dysplasia, 95% in caricinoma in situ, 100% in invasive carcinoma and 92% in total cases.
Signal transduction of 4-1BB for CD8+ T cell survival
Pakr, SJ,Choi, BK,Kim, HH,Nam, KO,Kwon, BS,Lee, HW 이화여자대학교 세포신호전달연구센터 2002 고사리 세포신호전달 심포지움 Vol. No.4
Engagement of two signals, T cell receptor(TCR) and co-stimulatory molecule, are necessary for proper activation of T cells. Co-stimulatory signals in T cell are provided by the corresponding ligands on antigen presenting cells(APC). TCR signal alone leads to T cell anergy. 4-1BB, one of co-stimulatory molecules, is a member of TNF receptor superfamily and expressed on T cells upon TCR ligation. It is well known that 4-1BB is co-stimulatory molecule enhancing proliferation and inhibiting activation-induced cell death(AICD) of T cells. Moreover, 4-1BB has been appreciated as a critical molecule for enhancing immune rejection in allograft transplantation, eradication of tumor, integrin-mediated T cell adhesion, and T cell-mediated cytolysis. It is reported that 4-1BB-mediated co-stimulation is involved in responses of CD4+ and CD8+ T cell To elucidate molecular mechanisms by which 4-1BB enhances proliferation and survival of T cells, we purified CD8+ T cells and investigated the effect of 4-1BB on T cell activation. 4-1BB stimulation increased proliferation and survival of CD8+ T cells. 4-1BB-mediated proliferation was inhibited by pharmacological blockers for NF B and PI3-kinase(PDTC and LY294002, respectively). 4-1BB stimulation up-regulated expression of anti-apoptotic gene, Bcl-X_(L) mRNA and protein, and the effect of 4-1BB on Bcl-X_(L) was blocked by NF B inhibitor, but not by PI3-kinase inhibitor. Beads conjugated with anti-CD3 plus anti-4-1BB antibody induced raft formation at the site where T cells contacts to beads, suggesting 4-1BB ligation recruits signaling molecules such as PKC and IKK to initiate T cell signaling. 4-1BB induced NF B activation as shown by I B degradation, whereas it did not increase phosphorylation of Akt, downstream kinase activated by PI-3 kinase. These data suggest that 4-1BB ligation activates signaling events in raft which results in CD8+ T cell activation. 4-1BB-evoked NF B activation may be responsible for up-regulation of Bcl-X_(L) expression, which could be one of mechanisms by which 4-1BB enhances survival and proliferation of T cells.
윤기영,조부관,공은희,안수미,김경은,김희정,장문경,이수현,신연명,최경현 KOSIN UNIVERSITY COLLEGE OF MEDICINE 2006 高神大學校 醫學部 論文集 Vol.21 No.1
Background/Aims: In recent years, intravenous catheters have been used in the hospitalized patients requiring fluids and medications, monitoring of the central venous pressure, and receiving the hyperalimentation. In spite of their usefulness in the management of the critically ill patients, the complications related to the intravenous catheters such as thrombosis, phlebitis, and sepsis have been well known. However, the actual incidence and bacterial pattern have not been well studied. This study was to investigate the difference in clinical way between colonized catheters and catheter-related bloodstream infections. Methods: From January 2004 to January 2005, 89 patients had done central venous catheters(CVC), receiving total parenteral nutrition(TPN) 51 and CVC for venous access 56 patients were included in this study. The patients ranged in age from 49 to 80 years, We evaluated 89 central venous catheterization patients were analyzed for blood culture, fever and catheter tip culture to identify the relationship between infectious complication and various clinical characteristics. Results: TPN formulas, indications of CVC, underlying diseases were associated with highter rates of positivity of blood cultures (P<0.05). Duration of CVC was related to the fever (P<0.05). No significant correlation was observed between duration of TPN and fever or blood cultures. Conclusions: TPN formulas and the duration of catheter dwelling time are associated with bacteremia and fever respectively. But this is more likely to be secondary to underlying diseases. Further study needs to be conducted in order to establish whether infectious complications are more related to TPN via CVC itself.