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Protein adhesion regulated by the nanoscale surface conformation
Cho, Eun Chul,Kong, Hyunjoon,Oh, Tae Byeong,Cho, Kilwon The Royal Society of Chemistry 2012 Soft matter Vol.8 No.47
<P>Protein adhesion and adsorption behaviors vary in response to variations in surface wettability; however, few reports have examined the dependence of such behaviors on variations in the surface molecular conformations. This study examines the degree to which molecular disorder at the surface of a surface-modified hydrocarbon chain monolayer regulates protein adhesion. Octadecyltrichlorosilane (OTS) molecules were deposited onto silicon wafers at two temperatures, 5 °C or 55 °C, to prepare two OTS surfaces with different degrees of molecular disorder. Atomic force microscopy (AFM) was used to evaluate the nanoscale adhesion force between proteins and the two types of OTS monolayers during a short contact time (<1 s). Bovine serum albumin (BSA) and human fibrinogen (HF) adhered more strongly to the disordered than to the ordered OTS monolayer. The adhesion strength at longer contact times (30 s–90 min) was evaluated by investigating the resistance of proteins on the OTS monolayer to detachment by washing. The magnitude of the resistance could be predicted from the topologies of the monolayers, as determined by AFM, after the adsorption of proteins and the subsequent washing experiments. After a 90 min adsorption period, BSA displayed a higher resistance to detachment from the disordered OTS monolayer than from the ordered OTS monolayer. HF displayed a higher resistance to detachment from the disordered OTS monolayer for only very short adsorption times of less than 1 min. The results suggest that the proteins altered their adhesion onto monolayers with different OTS conformations and that different adsorption times were required for each protein to present the different degrees of adhesion.</P> <P>Graphic Abstract</P><P>The molecular order of an underlying organic monolayer determines the adhesion behaviors of proteins onto these surfaces. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c2sm27204k'> </P>
조현준(Hyunjoon Cho),노영호(Youngho Rho),심동희(Donghee Shim) 한국정보기술학회 2009 한국정보기술학회논문지 Vol.7 No.1
MOST(Media Oriented System Transport) technology has been used to distribute audio, video and multimedia signals in serial and digital forms in a motor vehicle. In motor industry both 25MBPS MOST protocol and 50MBPS MOST protocol were opened except layer 1,2. 150 M BPS MOST protocol was not opened although it was developed. A bandwidth allocation method for an efficient streaming data transmission in MOST is proposed.
Media Oriented System Transport 네트워크에서의 UPnP 서비스 모델
조현준(Hyunjoon Cho),이철동(Chuldong Lee),심동희(Donghee Shim) 한국정보기술학회 2012 한국정보기술학회논문지 Vol.10 No.10
MOST(Media Oriented System Transport) network provides communication functions for applications and function blocks in MOST devices of vehicles. It supports 3 kinds of service, which are control message service, streaming data service and packet data service using control channel, synchronous channel and asynchronous channel respectively. The bandwidth of MOST network has been developed as 25MBPS, 50MBPS, 150MBPS. In MOST network of 150MBPS IP services are possible by directly MEP(MOST Ethernet packets) service using asynchronous channel, as the necessity of TCP/IP increases. UPnP(Universial Plug and Play) service model using MEP in MOST of 150 MBPS is designed logically in this paper. Simulation result shows the usefulness of this model.
조현준(Hyunjoon Cho) 한국정보과학회 1996 한국정보과학회 학술발표논문집 Vol.23 No.2B
지능망 응용프로토콜은 하부 프로토콜로써 No.7 신호방식의 TCAP(Transaction Capabilities Application Part)을 이용한다. 따라서 지능망 응용프로토콜의 ASE(Application Service Element)에서 제공하는 원격오퍼레이션(remote operation) 호출을 구현하기 위해서는 TCAP 서비스를 이용해야 하며, 이를 위하여 TCAP 서비스와 지능망의 ASE와 SACF(Single Association Control Function)에서 요구하는 원격오퍼레이션에 대한 기능분석이 요구된다. 본 논문에서는 이러한 필요성에 따라, TCAP의 서비스와 지능망에서 요구되고 있는 원격오퍼레이션의 특징에 대하여 알아보고, 지능망 응용프로토콜 구현을 TCAP 서비스 활용방안을 제안하고자 한다.
조남정(Namjeong Cho),최영윤(Youngyoon Cho),오형준(Hyunjoon Oh) 한국자동차공학회 2014 한국자동차공학회 학술대회 및 전시회 Vol.2014 No.11
Free Motion Headform (FMH) impact Test is US Federal Regulation to protect passenger being impacted to interior parts in car accident. but repeatability for this test is very sensitive and shows low repeatability. FMVSS201(u) requires that the upper vehicle interior structures with trim in a vehicle must satisfy FMH impact test with Head Injury Criterion(HIC) limit. To meet the FMVSS201U regulation, Energy absorbers need countermeasure between roof and headliner. In this paper, we defines control factor and noise factors and present the DFSS method to optimize Energy absorber. The focus of optimization is to reduce the cost and meet the performance target.
Cho, Ara,Ahn, SeJin,Yun, Jae Ho,Gwak, Jihye,Song, Hyunjoon,Yoon, Kyunghoon The Royal Society of Chemistry 2012 Journal of materials chemistry Vol.22 No.34
<P>A newly developed hybrid ink of binary CuS nanoparticles and In precursor solution was prepared to form a CuInSe<SUB>2</SUB> (CIS) thin film. Previously, we have shown a CIS thin film solar cell with 4.19% conversion efficiency using the hybrid ink of Cu<SUB>2−<I>x</I></SUB>Se nanoparticles and In precursor. Deposition using hybrid ink offers advantages including the provision of stress-relief and crack-deflection centers by pure material based nanoparticles and effective binding with the nanoparticles by precursor solutions without other organic binders. Here, we demonstrate volume expansion of a thin film for forming a well-grown absorber layer using CuS nanoparticles instead of Cu<SUB>2−<I>x</I></SUB>Se. Binary nanoparticles were synthesized by a low temperature colloidal process and a precursor solution was prepared by using a non-toxic chelating agent to disperse the In component stably. The band gap of the CIS thin film was 1.08 eV, as determined by external quantum efficiency (EQE) measurements, and the reproducible conversion efficiency of the fabricated device was 6.23%.</P> <P>Graphic Abstract</P><P>A CIS thin film was produced with a newly developed hybrid ink of synthesized binary CuS nanoparticles and an In precursor solution in a non-vacuum system. During the process, S in CuS was replaced by Se vapor and a dense CIS thin film was then formed <I>via</I> volume expansion due to the replacement of S with Se. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c2jm32624h'> </P>