Speed Optimization in Automated Microinjection of Zebrafish Embryos

Peter C.Y. Chen,Shengfeng Zhou,Zhe Lu,Joo-Hoo Nam,Hong Luo,RuowenGe,Chong-Jin Ong,Wei Lin 제어·로봇·시스템학회 2015 International Journal of Control, Automation, and Vol.13 No.5

In this paper we formulate an optimization problem in the design of a speed trajectory for the motion of the micropipette during automated microinjection of zebrafish embryos. The objective of this optimization problem is to minimize the deformation sustained by the zebrafish embryo. We subsequently propose a solution to this optimization problem by first constructing a viscoelastic model of the zebrafish embryo, and then synthesizing an optimal speed trajectory based on a class of polynomials. Furthermore, we present results of numerical simulation and experiments that demonstrate the effectiveness of the proposed solution. The statistically meaningful experimental data (generated using a large sample of zebrafish embryos) provide direct evidence on the advantage of such speed optimization in microinjection.

Development and Validation of an Integrated Healthy Workplace Management Model in Taiwan

Chen Fu-Li,Chen Peter Y.,Chen Chi-Chen,Tung Tao-Hsin 한국산업안전보건공단 산업안전보건연구원 2022 Safety and health at work Vol.13 No.4

Background: Impacts of exposure are generally monitored and recorded after injuries or illness occur. Yet, absence of conventional after-the-effect impacts (i.e., lagging indicators), tend to focus on physical health and injuries, and fail to inform if workers are not exposed to safety and health hazards. In contrast to lagging indicators, leading indicators are proactive, preventive, and predictive indexes that offer insights how effective safety and health. The present study is to validate an extended Voluntary Protection Programs (VPP) that consists of six leading indicators. Methods: Questionnaires were distributed to 13 organizations (response rate ¼ 93.1%, 1,439 responses) in Taiwan. Cronbach a, multiple linear regression and canonical correlation were used to test the reliability of the extended Voluntary Protection Programs (VPP) which consists of six leading indicators (safe climate, transformational leadership, organizational justice, organizational support, hazard prevention and control, and training). Criteria-related validation strategy was applied to examine relationships of six leading indicators with six criteria (perceived health, burnout, depression, job satisfaction, job performance, and life satisfaction). Results: The results showed that the Cronbach’s a of six leading indicators ranged from 0.87 to 0.92. The canonical correlation analysis indicated a positive correlation between the six leading indicators and criteria (1st canonical function: correlation ¼ 0.647, square correlation ¼ 0.419, p < 0.001). Conclusions: The present study validates the extended VPP framework that focuses on promoting safety and physical and mental health. Results further provides applications of the extended VPP framework to promote workers’ safety and health.

A review of rotorcraft Unmanned Aerial Vehicle (UAV) developments and applications in civil engineering

Peter Liu,Albert Y. Chen,Yin-Nan Huang,Jen-Yu Han,Jihn-Sung Lai,Tzong-Hann Wu,Ming-Chang Wen,Meng-Han Tsai,Shih-Chung Kang 국제구조공학회 2014 Smart Structures and Systems, An International Jou Vol.13 No.6

Civil engineers always face the challenge of uncertainty in planning, building, and maintaininginfrastructure. These works rely heavily on a variety of surveying and monitoring techniques. Unmannedaerial vehicles (UAVs) are an effective approach to obtain information from an additional view, andpotentially bring significant benefits to civil engineering. This paper gives an overview of the state of UAVdevelopments and their possible applications in civil engineering. The paper begins with an introduction toUAV hardware, software, and control methodologies. It also reviews the latest developments in technologiesrelated to UAVs, such as control theories, navigation methods, and image processing. Finally, the paperconcludes with a summary of the potential applications of UAV to seismic risk assessment, transportation,disaster response, construction management, surveying and mapping, and flood monitoring and assessment.

A review of rotorcraft Unmanned Aerial Vehicle (UAV) developments and applications in civil engineering

Liu, Peter,Chen, Albert Y.,Huang, Yin-Nan,Han, Jen-Yu,Lai, Jihn-Sung,Kang, Shih-Chung,Wu, Tzong-Hann,Wen, Ming-Chang,Tsai, Meng-Han Techno-Press 2014 Smart Structures and Systems, An International Jou Vol.13 No.6

Civil engineers always face the challenge of uncertainty in planning, building, and maintaining infrastructure. These works rely heavily on a variety of surveying and monitoring techniques. Unmanned aerial vehicles (UAVs) are an effective approach to obtain information from an additional view, and potentially bring significant benefits to civil engineering. This paper gives an overview of the state of UAV developments and their possible applications in civil engineering. The paper begins with an introduction to UAV hardware, software, and control methodologies. It also reviews the latest developments in technologies related to UAVs, such as control theories, navigation methods, and image processing. Finally, the paper concludes with a summary of the potential applications of UAV to seismic risk assessment, transportation, disaster response, construction management, surveying and mapping, and flood monitoring and assessment.

Stimuli-Induced Equilibrium Point: A Psychological Field Theory Application in Ramp Merging Systems

Kendrick Amezquita S.,Peter C. Y. Chen,Weihai Chen,Zheng Zhao 제어로봇시스템학회 2017 제어로봇시스템학회 국제학술대회 논문집 Vol.2017 No.10

Based on the psychological field theory, the stimuli-induced equilibrium point (SIEP) model is formulated to characterize the interaction of a driver between two vehicles (leader and follower) in a three-vehicle platooning configuration. Considering the intensity of the stimuli that act upon a driver between these vehicles, the point at which the stimuli resultant becomes zero (i.e. the equilibrium point) is obtained. Consequently, the location of such a point within the leader-follower gap changes continuously according to the speed of the middle vehicle and the current traffic scenario. This methodology enables the systematic computation of speed and position references for automatic ramp merging systems. Such stimuli-induced equilibrium point has shown to improve the performance of existing merging control schemes while increasing safety conditions by providing enough reaction time for drivers to avoid and eventual collision.

A Mn(iv)-peroxo complex in the reactions with proton donors

Lee, Chien-Ming,Sankaralingam, Muniyandi,Chuo, Chi-He,Tseng, Tzu-Hsien,Chen, Peter P.-Y.,Chiang, Ming-Hsi,Li, Xiao-Xi,Lee, Yong-Min,Nam, Wonwoo The Royal Society of Chemistry 2019 Dalton Transactions Vol.48 No.16

<P>Protons play an important role in promoting O-O or M-O bond cleavage of metal-peroxo complexes. Treatment of side-on O2-bound [PPN][Mn<SUP>IV</SUP>(<SUP>TMS</SUP>PS3)(O2)] (1, PPN = bis(triphenylphosphine)iminium and <SUP>TMS</SUP>PS3H3 = 2,2′,2′′-trimercapto-3,3′,3′′-tris(trimethylsilyl)triphenylphosphine) with perchloric acid (HClO4) in the presence of PR3 (R = phenyl or <I>p</I>-tolyl) results in the formation of neutral five-coordinate Mn<SUP>III</SUP>(OPR3)(<SUP>TMS</SUP>PS3) complexes (R = phenyl, 2a; <I>p</I>-tolyl, 2b), which are confirmed by X-ray crystallography. Isotope labelling experiments demonstrate that the oxygen atom in the phosphine oxide product derives from the peroxo ligand of 1. Reactions of 1 with weak proton donors, such as phenylthiol, phenol, substituted phenol and methanol, are also investigated to explore the reactivity of the Mn<SUP>IV</SUP>-peroxo complex, leading to the isolation of a series of five-coordinate [Mn<SUP>III</SUP>(L)(<SUP>TMS</SUP>PS3)]<SUP>−</SUP> complexes (L = phenylthiolate, phenolate or methoxide). Mechanistic aspects of the reactions of the Mn<SUP>IV</SUP>-peroxo complex with proton donors are discussed as well.</P>

Optimization of a Fluid Distribution Manifold

Heng Wang,Samuel D. Marshall,Rerngchai Arayanarakool,Lakshmi Balasubramaniam,Xin Jin,Poh Seng Lee,Peter C. Y. Chen 한국유체기계학회 2018 International journal of fluid machinery and syste Vol.11 No.3

This paper presents two optimized designs of a commonly-used fluid distribution manifold having one entrance and six exits. Numerical simulations were carried out to optimize the dimensions and mechanisms of these proposed designs for the sake of enhancing the uniformity of fluid distribution amongst the exits and reducing the formation of dead zones inside the manifold cavities. Particularly, to make the fluid distribution amongst exits more uniform, this study explored the relationship between entrance diameter and exit diameter. Furthermore, in order to reduce dead zone formations inside the manifold whilst still maintaining uniform fluid distribution, a conical cavity was designed. After that, blockers were designed to replace some exits, permitting a variable number of fluid distribution manifold exits, depending on the specific application. Both designs were found to be able to improve flow uniformity and dead zone reduction compared to the original commonly-used fluid distribution manifold, with the central-feeding distributor performing slightly better than the lateral-feeding distributor overall. From the perspective of manufacturing, each of these two fluid manifolds was made of two pieces with glue and rubber O ring used respectively as the bond between separate pieces. Preliminary experiments with these devices suggest similar results to those from the numerical studies. Based on real application requirements and limitations, the different fluid manifold designs with tunable dimensions can be utilized in various mechanical or biochemical devices to distribute fluid equally amongst several parallel components.