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사용자경험 단계를 고려한 지능형 헬스케어 서비스 제안 : 지속 가능한 습관 형성을 중심으로
유채화(Chaehwa Yoo),배희준(Heejun Bae),이재은(Jaeeun Lee),박세익(Seik Park),김민정(Minjeoung Kim),이주환(Ju-Hwan Lee) 한국HCI학회 2022 한국HCI학회 학술대회 Vol.2022 No.2
전세계적 팬데믹이 발생한 2020 년 이후로 우리의 일상적인 삶의 양식이 크게 달라지며 많은 영역이 온라인을 중심으로한 비대면 활동으로 치환되었다. 혼자서도 건강한 일상적인 삶을 영위할 수 있는 시간, 감정, 의지와 같은 자기 관리 능력의 중요성이 부상하고 있으며 동시에 자기 관리 능력의 부족으로 어려움을 겪는 이들 역시 늘어나고 있다. 이에 자기 관리 트렌드를 주도 하고 있는 1 인가구 MZ 세대를 대상으로하여 건강한 습관 형성의 주요 요인이 무엇인지 분석하고, 문제점을 해결하여 건강한 습관을 만드는 서비스를 제안하고자 한다. 제시한 건강 관리 습관 형성 서비스 ‘해빗 투게더(habit together)’는 주변 지인과 함께할 수 있는 건강 습관 형성 서비스로 기존 습관 형성 어플들과 달리 지능적 자동 기록 방식으로 건강 관련 데이터를 기록하고 실시간으로 공유한다. 나아가 개인화된 건강 습관을 제시하여 기존의 문제 상황을 해결하고자 한다.
Isolation and Characterization of a Protease Inhibitor from Drosophila melanogaster
Dongmin Kang,Chaehwa Park,Jeongbin Yim 생화학분자생물학회 1994 BMB Reports Vol.27 No.4
A trypsin inhibitor was purified 4,100-fold to apparent homogeneity from Drosophila melonogoster. The molecular weight of the purified protein inhibitor was estimated to be 8,000 daltons by gel permeation chromatography, and 8,500 daltons by sodium dodesyl sulfate-polyacrylamide gel electrophoresis. The inhibitor forms a complex with bovine pancreatic trypsin at a molar ratio of 1 : 1. This protein can inhibit other proteases, such as porcine pancreatic elastase and collagenase, but not chymotrypsin, subtilisin, papain, or pepsin. The inhibitor retained its activity over a broad range of pH (1 to 12) and was relatively thermostable.
친환경 흑염소 사양을 위한 최적 조사료 초종 및 TMR 혼합비 비율: In vitro 반추위 발효 연구
류채화 ( Ryu Chaehwa ),이진욱 ( Lee Jinwook ),김관우 ( Kim Kwan-woo ),이성수 ( Lee Sung-soo ),박혜련 ( Bak Hyeryeon ),전은정 ( Jeon Eunjeong ),박명선 ( Park Myungsun ),최낙진 ( Choi Nag-jin ) 한국유기농업학회 2020 韓國有機農業學會誌 Vol.28 No.4
This study was conducted to investigate the effects of TMR on in vitro rumen fermentation and methane production of goat with different forage sources. The experiment was arranged 4×2 factorial design. The different forage sources were rice straw (RS), Italian rye grass (IR), timothy (TI) and alfalfa (AL), respectively. There were two different forage : concentrate ratios such as 20:80 (20) and 50:50 (50), respectively. Therefore, totally 8 treatments were used: 1) RS20, 2) RS50, 3) IR20, 4) IR50, 5) TI20, 6) TI50, 7) AL20, and 8) AL50, respectively. The rumen fluid of goat was collected from the slaughterhouse. For fermentation parameters, ruminal pH, total gas, methane, hydrogen, ammonia nitrogen, and volatile fatty acid were determined. The pH values were within an optimal range across all treatments. Total gas productions at TI20 and AL50 were significantly greater than others (p<0.05). Methane production was significantly lower in TI and AL compared with other treatments (p<0.05). The relatively high dietary NDF content in treatments showed significantly lower methane production (p<0.05). Significant alterations treatments were detected at ammonia nitrogen concentration according to the ratio of forage : concentrate (p<0.05). AL treatment showed greater total volatile fatty acid production compared with other treatments (p<0.05). Therefore, the present study suggests that both Timothy and Alfalfa could be recommendable forage sources for goat based on results with volatile fatty acid as an energy source and methane as an index for energy loss and environmental issues. Also, the 50:50 (forage : concentrate) ratio would prefer to 20:80.
유경주,Ji Young Lee,Chaehwa Park,Duck Cho,Seok Jin Kim 대한진단검사의학회 2020 Annals of Laboratory Medicine Vol.40 No.3
Methods for reproducibly isolating and enriching small extracellular vesicles (EVs) from blood are essential for clinical utilization of small EVs in cancer patients. We combined ultracentrifugation (UC) with polymer-based precipitation (ExoQuick [EQ] or Total Exosome Isolation [TEI] kit) to isolate small EVs (diameter, 30–150 nm) from the serum of breast cancer patients. We compared the performance of four cycles of UC (UC4x) with that of two cycles of UC followed by enrichment using the EQ (UC2x→EQ) or TEI (UC2x→TEI) kits. The mean concentration of small EVs isolated from 1 mL of serum using UC2x→EQ (139.0±29.1 μg) and UC2x→TEI (140.4±5.0 μg) did not differ from that obtained using UC4x (141.8±26.9 μg). The mean number of EV particles obtained using UC4x was 29.2± 9.9×109 per mL of serum, whereas UC2x→EQ and UC2x→TEI yielded higher numbers of EVs (50.7±17.0×109 and 59.3±20.6×109, respectively). Concentrations of EV microRNAs, including miR-21 and miR-155, did not differ between the three methods. In conclusion, performing UC prior to the use of polymer-based precipitation kits could be feasible for isolating small EVs from human serum in large sample-based translational researches.
Differential effects of RhoA signaling on anticancer agent-induced cell death.
Kang, Won Ki,Lee, Inkyoung,Ko, Ukyoung,Park, Chaehwa National Hellenic Research Foundation 2005 ONCOLOGY REPORTS Vol.13 No.2
<P>Substantial evidence exists to support a role for RhoA signaling in adhesion and cytoskeletal reorganization, while relatively less is known about the participation of RhoA on cell survival. We provide evidence that RhoA functions as a differential modulator of apoptosis induced by anticancer agents. Specifically, both RhoA and caRhoA induce statistically significant resistance to statin, etoposide, 5-FU and taxol while increasing sensitivity to vincristine (all p<0.001). The IC50 values for statin, etoposide, 5-fluorouracil (5-FU) and taxol in caRhoA transfectant were 8.70+/-0.74, 4.08+/-0.12, 4.12+/-0.12 microg/ml and 3.84+/-0.16 ng/ml, respectively, whereas the respective IC50 values in the mock-transfected control were 3.40+/-0.21, 1.12+/-0.06, 1.21+/-0.06 microg/ml and 2.84+/-0.15 ng/ml. This represented a 2.6-, 3.5-, 3.2- and 1.4-fold resistance to statin, etoposide, 5-FU and taxol, respectively. In contrast, caRhoA increased sensitivity to vincristine, decreasing IC50 values from 4.61+/-0.46 to 3.73+/-0.44 ng/ml (p<0.001). Western blot analysis demonstrated that RhoA mediates induction of E2F-1, Cdk2 and PCNA, accompanying concurrent reduction in p21 and p27. However, cleavage assays of poly (ADP-ribose) polymerase, BID, caspase-8 and caspase-3 indicate that the cell growth modulation mediated by RhoA in response to these anticancer agents occurs through the inhibition of apoptosis. Taken together, these results indicate that RhoA differentially modulates cancer cell death depending on the anticancer agent.</P>
Yeom, Seon-Yong,Nam, Do-Hyun,Park, Chaehwa American Association for Cancer Research 2014 Molecular Cancer Therapeutics Vol.13 No.12
<P>Glioblastoma multiforme (GBM) is an extremely aggressive brain cancer with a median survival of less than 2 years. GBM is characterized by abnormal activation of receptor tyrosine kinase and constitutively activated STAT3. Although EGFR phosphorylation and STAT3 activation are essential for the maintenance of GBM cancer stem cells, the molecular mechanism underlying endosome-mediated STAT3 activation is not fully understood. In the current study, we showed that GTP-binding protein RRAD (RAS associated with diabetes, RAD) physically associates with EGFR, and EEA1, enhancing the stability and endosome-associated nuclear translocation of EGFR. Functionally, RRAD contributes to the activation of STAT3 and expression of the stem cell factors OCT4, NANOG, and SOX2, thereby enhancing self-renewing ability, tumor sphere formation, EMT, and <I>in vivo</I> tumorigenesis. Most importantly, RRAD contributes to poor survival in patients with GBM. RRAD expression is correlated with temozolomide resistance, and, conversely, depletion of RRAD leads to sensitization of highly temozolomide-resistant GBM cells. Our data collectively support a novel function of RRAD in STAT3 activation and provide evidence that RRAD acts as a positive regulator in the EGFR signaling pathway. These results demonstrate a critical role for RRAD in GBM tumorigenesis and provide a rationale for the development of pharmacologic inhibitors of RRAD in GBM. <I>Mol Cancer Ther; 13(12); 3049–61. ©2014 AACR</I>.</P>
Characterization of RhoA-mediated Chemoresistance in Gastric Cancer Cells
Won Ki Kang,Inkyoung Lee,Chaehwa Park 대한암학회 2005 Cancer Research and Treatment Vol.37 No.4
Purpose: RhoA is a critical transducer of extracellular signals, which leads to organization of actin cytoskeleton, motility, adhesion and gene regulation. The present study aimed to explore whether RhoA influences the susceptibility of gastric cancer cells to chemotherapeutic drugs.Materials and Methods: SNU638 cells were transfected with a mock vector (pcDNA3.1), RhoA (pcDNA/RhoA), or constitutively active RhoA (pcDNA/caRhoA). MTT assay and Western blot analysis were performed to study the growth response to several chemotherapeutic drugs in the gastric cancer cell line, SNU638, with different RhoAlevels.Results: RhoA significantly enhanced the resistance to lovastatin, 5-FU, taxol and vincristine, but did not affect the sensitivity to cisplatin or etoposide in SNU638. In theWestern blot analysis, RhoA decreased the PARP cleavage, which was accompanied by a concurrent reductionin cell death. The gene expression profile after a cDNA microarray analysis demonstrated that RhoA was associated with the differential expression of 19 genes, including those involved in anti-oxidant defense, glucose metabolism, anti-apoptosis and protein turnover.Conclusion: Gastric cancer cells with a high expression of RhoA could be resistant to chemotherapeutic drugs, such as taxol or vincristine, implying that treatment strategiesaimed at inactivation of RhoA might be promising for improving the efficacy of these chemotherapeutic drugs.