Cadmium과 Di-(2-ethylhexyl) Phthalate에 의한 고환 독성 : 아연과 Apoptosis의 역할 The Role of Zinc and Apoptosis

권민,최병선,박언섭,홍연표,박정덕 중앙대학교 의과대학 의과학연구소 2003 中央醫大誌 Vol.28 No.1

Cadmium (Cd) and di-(2-ethylhexyl) phthalate are used widely and distributed ubiquitously in the environment, which are well-known reproductive toxicants. DEHP and/or Cd causes the apoptosis, degeneration of spermatogenic cells, and massive necrosis, resulting in testicular atrophy. Zinc is an essential metal in spermatogenesis, the zinc concentration in testis is changed in DEHP- or Cd-treated animals. However, the role of zinc or apoptosis in testicular toxicity by DEHP or Cd has not been understood well. In this study, Sprague-Dawley male rats were given DEHP (2 g/kg, 5 ml corn-oil, PO) or Cd (CdCl₂2 mg/kg, 4 ml saline, sc) for 1, 2, 7 and 14 days. Control rats were given vehicle corresponding to each group. At day 1, 2, 7, and 14 after administration of DEHP or Cd, the morphological findings including apoptosis in testes were observed under the light microscope, and the zinc concentrations in testes were determined by atomic absorption spectrometry with flame method. Fas and FasL mRNA in testes were analyzed by RT-PCR method at 12 hours, 1, 7, 14 days after adminstration of DEHP or Cd. Absolute and relative testicular weight were lower in DEHP- or Cd-treated rats than control. DEHP or Cd produced morphological changes in testes, including apoptosis, degeneration, necrosis and massive loss of spermatogenic cells, resulting in testicular atrophy. The zinc concentration in testes was not different at day 1 after administration of DEHP, but was decreased at later times. Testicular zinc was lower at day 1, 2 in Cd-treated rats than control, but was higher at 7, 14 days after Cd administration. The levels of Fas and FasL mRNA were highly expressed in testes after administration of DEHP or Cd. These results suggest that the change of zinc level in testes might be a secondary effect of DEHP- or Cd-induced testicular toxicity, and Fas signal system might be play an important role in pocessing of apoptosis in testes by DEHP or Cd.

福祉國家를 위한 社會體育의 活性化 方案에 관한 硏究

송병권,최철연 長安大學 地域硏究所 1994 地域硏究所 論文集 Vol.3 No.1

신규개발된 제초제 metamifop 현탁농축액제에 대한 급성독성평가

이동연,이상훈,백경진,김태원,정두순,임종환,김명석,박병권,윤효인 충남대학교 수의과대학 동물의과학연구소 2004 動物醫科學硏究誌 Vol.12 No.-

Metamifop, (R)-2-[4-[(6-chloro-2-benzoxazolyloxyphenoxy]-N-(2-fluorophenyl)-N-methyl propanamide, was newly developed by Dongbu Hannong Chemicals in Korea. In the present study we conducted acute oral toxicity and acute dermal toxicity test. The values of LD_(50) of metamifop was found to be over 5,000 mg/kg for ICR mice. On the other hand, metamifop showed no acute dermal toxicity to SD rats.

Carbosulfan의 피부자극성, 안점막 자극성 및 피부감작성등의 알러지성 효과에 관한 연구

이상훈,이동연,백경진,김태원,정두순,임종환,김명석,박병권,윤효인 충남대학교 수의과대학 동물의과학연구소 2004 動物醫科學硏究誌 Vol.12 No.-

Many studies have been published on the human health effects of carbosulfan toxicity. Carbosulfan used as an insecticide can produce an irritating and allergenic effect when acting on the skin. In this study, a sensitization test on albino guinea pigs and intradermal reactivity and occular irritation test on albino rabbits were carried out with carbosulfan. Signs of acute skin inflammation appeared along with the clinical symptom of chronic dermatosis caused by the solution irritating characteristics. Moreover, carbosulfan showed severe eye irritation with conjuctival redness and severe opacity on cornea. Preventive measures should involve workers' obligatory instructions on the noxious impact on human skin and strict observance of industrial safety rules.

Pyriminobac-methyl과 azimsulfuron 합제의 신규제형인 분산형 정제에 대한 독성 평가

백경진,이동연,이상훈,김태원,정두순,임종환,김명석,박병권,윤효인 충남대학교 수의과대학 동물의과학연구소 2004 動物醫科學硏究誌 Vol.12 No.-

Dispersible tablets disintegrate either to form a stabilized suspension rapidly in water, or to disperse instantaneously in the rice field without the aid of water. In this study, the newly formulated dispersible tablet form of herbicidal combination, pyriminobac-methyl and azimsulfuron, was evaluated the safety for human and animals in the toxicological viewpoints. The LD_(50) value of dispersible tablet form for pyriminobac-methyl and azimsulfuron was above 2,500 mg/kg for the oral route and above 2,000 mg/kg for the dermal route. It is considered to have low acute oral and dermal toxicity based on the findings of the present studies. In addition, the combination of pyriminobac-methyl and azimsulfuron showed mild skin irritant under the conditions of this study and conjunctival redness and slight opacity on cornea appeared from 1 hour after application.

관상동맥 질환에서 CD14 유전자형에 따른 세균 및 Heat Shock Protein에 대한 반응의 차이

한주용,최수연,조현주,김화평,강현재,구본권,김남중,김효수,손대원,오병희,박영배,최윤식 대한감염학회 2007 감염과 화학요법 Vol.39 No.1

Background : CD14 is the receptor for lipopolysaccharides and heat shock protein (HSP), which has been suggested being associated with increased risk of coronary artery disease (CAD). We investigated whether the response to infectious agents or HSP is different according to CD14 polymorphism in Koreans. Materials and Methods : Antibody titers to Helicobacter pylori, Chlamydia pneumoniae, and human HSP60 (hHSP60) were measured in 48 patients with stable CAD and in 41 healthy controls by ELISA. CD14 genotype was determined by PCR and high-sensitivity C-reactive protein (hs-CRP) was measured. Results : Seropositivity to C. pneumoniae and H. pylori, and antibody titer to hHSP60 were not significantly associated with the presence of CAD. CD14 genotype distribution was 31 TT (35%), 43 CT (48%), and 15 CC (17%). To compare the response to the infectious organism and hHSP60, we divided study population into 3 groups; CAD patients with non-TT genotype (group I, n=30), CAD patients with TT genotype (group II, n=18), and normal controls (group III, n=41). Seropositivity to C. pneumoniae and H. pylori, and antibody titer to hHSP60 were not significantly different among 3 groups. Though hs-CRP level was significantly different among 3 groups, post-Hoc analysis showed that hs-CRP level was not significantly different between group I and group II (group I: 1.6[1.1-3.5] mg/L and group II: 0.35[0.1-2.0] mg/L). Conclusions : This study suggests that the inflammatory responses to infectious organisms and HSP do not differ according to the CD14 genotype in Koreans. 목적 : 만성적인 세균 감염이나 자가 면역 반응이 동맥경화와 연관되어 있다는 주장이 제기되어 왔다. CD14은 lipopolysaccharides (LPS)와 heat shock protein(HSP)의 수용체로 C(-260)→T 다형성이 관상동맥 질환의 위험과 관련되어 있다고 제안되어 왔다. 본 연구에서는 한국인에서 CD14 다형성에 따른 LPS와 HSP에 대한 반응성을 염증표지자를 측정하여 살펴보고자 하였다. 재료 및 방법 : 안정형 관상동맥 환자 48명과 정상 대조군 41명을 대상으로 Chlamydia pneumoniae, Helicobacter pylori 항체 및 인체 HSP60 (hHSP60) 항체 역가를 enzyme-linked immunosorbent assay (ELISA) 방법으로 측정하였다. CD14 유전자형은 중합효소연쇄반응을 이용하여 결정하였고 hs-CRP를 측정하였다. 결과 : C. pneumonias 및 H. pylori 항체 양성 여부 및 항체 역가, 그리고 hHSP60에 대한 항체 역가는 안정형 관상동맥 질환의 유무와 유의한 상관관계는 없었다. CD14 유전자형의 분포는 TT 30명(39%), CT 31명(40%), 그리고 CC 16명(21%)이었다. 관상동맥 질환 군에서 TT 유전자형은 38%, 대조군에서는 32%로 TT 유전자형과 관상동맥 질환의 유무와는 유의한 상관관계가 발견되지 않았다(P>0.05). CD14 유전자형에 따라 C. pneumonias와 H.pylori 감염 및 hHSP60에 따른 반응에 차이가 있는지 여 부를 알아보기 위해, 관상동맥 질환 군을 다시 TT 유전자형 군과 non-TT 유전자형 군으로 나누어, 전체적으로 세군에서 항체 양성률과 역가를 비교하였다(I군: CD14 TT 유전자형을 가진 안정형 관상동맥 환자, II군: CD14 TT 유전자형을 가진 안정형 관상동맥 환자, III군: 정상 대조군). 세 군에서 C. pneumonias와 H. pylori 항체 양성률 및 hHSP60 항체 역가에는 유의한 차이가 없었다. 세 군사이에 hs-CRP 값에 유의한 차이가 있었지만, post-Hoc분석에서 II군의 hs-CRP 값이 I군에 비해 유의하게 높지는 않았다(0.35 [0.1-2.0] mg/L in group II vs. 1.6 [1.1-3.5]mg/L in group I, P>0.05). 결론 : 본 연구는 한국인에서 CD14 유전자형에 따라 LPS나 HSP에 대한 반응이 유의하게 다르지 않음을 시사한다.

한국인 주요우울장애 환자에서 혈중 ACTH, Cortisol 농도와 해밀턴 우울 평가 척도의 신체증상 항목과의 상관관계

여혜빈,김린,함병주,심세훈,권영준,정희연,정한용,한상우,이민수,이화영 대한생물치료정신의학회 2011 생물치료정신의학 Vol.17 No.2

Objectives:Hypothalamic-Pituitary-Adrenocortical(HPA) system dysregulation plays an important role in the pathophysiology of major depressive disorder. Medically unexplained somatic symptoms comprised the predominant complaints of korean patients with major depressive disorder. It might be related to dysregulation of HPA system and somatic symptoms. The aim of this study was to investigate the association between Hamilton Depression Rating Scale severity and the Plasma Adrenocorticotropic Hormone(ACTH) & Serum Cortisol level in Korean patients with major depressive disorder. Methods:Our study design was prospective. A total 111 hospitalized patients with major depressive disorder (DSM-IV) at Anam Hospital, Korea University Medical Center between Jan 2009 and May 2011 were selected. Hamilton Depression Rating Scale was measured by psychiatrist. Plasma ACTH, Serum Cortisol levels were analyzed at the time of admission. Results:There are significant correlations(p<0.05) between Hamilton Depression Rating Scale of somatic component(HAM-D 11-15 item) and Plasma ACTH and Serum Cortisol level. However, other HAM-D sub items were not significantly correlated with Plasma ACTH and Serum Cortisol level except somatic component. Conclusion:Our Study results suggest that hyperactivity of HPA axis might be correlation with somatic symptoms in korean patients with major depressive disorder.

Enterococcus faecium에서 Vitek GPS Card의 Imipenem에 대한 위감수성

이혜경,박연준,궈희정,이은정,김병기,강창석 대한임상검사정도관리협회 2003 臨床檢査와 精度管理 Vol.25 No.2

액체크로마토그래피/질량분석법을 이용한 랫트 혈장내의 Surfactin C의 정량법에 관한 연구

임종환,김명석,이동연,이상훈,백경진,김태원,정두순,박병권,윤효인 충남대학교 수의과대학 동물의과학연구소 2004 動物醫科學硏究誌 Vol.12 No.-

Surfactin isolated from Bacillus subtilis has a strong surface tension-lowering activity, together with antiviral, antitumor, fibrinolytic and hypocholesterolemic activities. LC/MS is a simple, rapid and effective technique for the determination of surfactin C in rat serum. The separation of surfactin was achieved on reverse phase column. The mobile phase consisted of 0.1% trifluoracetic acid in water (A) and acetonitrile (B). As a result of analysis of blank rat serum samples, matrix interference was not shown. The values of precision and accuracy were satisfied w the guideline. A newly developed LC/MS method for the determination of surfactin C was successfully applied in its pharmacokinetic analysis.

Effects of grain-based pecking blocks on productivity and welfare indicators in commercial broiler chickens

Kwon Byung-Yeon,Lee Hyun-Gwan,Jeon Yong-Sung,Song Ju-Yong,Park Jina,Kim Sang-Ho,Kim Dong-Wook,이경우 아세아·태평양축산학회 2024 Animal Bioscience Vol.37 No.3

Objective: This experiment was conducted to investigate the effect of grain-based pecking blocks on productivity and welfare status at two commercial broiler welfare-certified farms. Methods: Production and welfare indicators were assessed at two farms (designated Farm A and B). Both farms had two windowless houses with forced tunnel-type ventilation and housed broilers at stocking densities of approximately 16.7 birds/m2 (Farm A) and 16.8 birds/m2 (Farm B). Each house was divided into two or three equal sections and was provided with or without pecking blocks. Grain-based pecking blocks, measuring 25 × 25 × 25 cm, were given to broilers in both farms at 1 block per 1,000 birds. Various parameters including productivity (body weight and flock uniformity), corticosterone levels (in fecal droppings and feathers), footpad dermatitis, hock burn, feather dirtiness, gait score, litter quality, body surface temperature, and volatile fatty acids in fecal samples were assessed at 26 days of age, whereas litter quality was analyzed at 13 and 26 days of age. Results: There were no significant effects of providing pecking blocks on productivity (body weight and uniformity), fecal and feather corticosterone, welfare indicators (i.e., footpad dermatitis, hock burn, feather cleanliness, and gait score), and litter quality (i.e., moisture, nitrogen, and pH). No differences in body surface temperature between the control and enrichment treatments were noted in Farm B, but body surface temperatures of the head (p = 0.029) and legs (p = 0.011) in the enrichment vs. control group were elevated in Farm A. Butyrate concentration in the enrichment vs control group was higher in Farm B (p = 0.023), but this effect was not detected in Farm A. Conclusion: It is concluded that grain-based pecking blocks did not affect performance and welfare indicators. Further studies are warranted to elucidate the potential impact of grain-based pecking blocks on gut health indicators.