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김우영,노병만 효성여자대학교 법정연구소 1998 법정연구 Vol.5 No.-
The subject of this thesis is on causes and reform plan of corruption of thepower. It is analyzed in the viewpoint of structure and system. As causes for corruption of the public office, in administrative system, it isscrutinized five elements such as ⅰ) control-oriented administration by theadministrative convenience ⅱ) ambiguity of the administratine regulation ⅲ) thesecret administrative procedure ⅳ) unstrict. supervision ⅴ) interest-group tendencyof bureaucracy. And in environment of administrative system, it is understood suchas ⅰ) weakness of the political structure ⅱ) promodern economical structure ⅲ)interest-group tendency of the legal profession ⅳ) weakness of the press structure. As reform plan for corruption of the public office, it is pointed out such as, inadministrative system ⅰ) control-oriented administrative overthrow by theadministrative convenience ⅱ) simplification and transparency of the administrativeprocedure ⅲ)the strict inspection, and in environment of the administrative system,ⅰ) reformation of the political sfructure ⅱ) reformation of the legal profession ⅲ)transparency improvement of the enterprise ⅳ) weakness improvement of the pressagency.
Simple Purification of a Foreign Protein Using Polyhedrin Fusion in a Baculovirus Expression System
ROH, Jong Yul,CHOI, Jae Young,KANG, Joong Nam,WANG, Yong,SHIM, Hee Jin,LIU, Qin,TAO, Xueying,XU, Hong Guang,HYUN, Jin-Ho,WOO, Soo Dong,JIN, Byung Rae,JE, Yeon Ho Japan Society for Bioscience, Biotechnology, and A 2010 Bioscience, Biotechnology, and Biochemistry Vol.74 No.8
<P>Previously, we found that expression by translational fusion of the polyhedrin (Polh)-green fluorescence protein (GFP) led to the formation of granular structures, and that these fluorescent granules were easily precipitated by high-speed centrifugation. Here, we developed an easy, fast, mass purification system using this baculovirus expression system (BES). An enhanced GFP (EGFP) fused with the Polh gene at the N-terminus, including a linker and enterokinase (EK) site between Polh and EGFP, was expressed in Sf9 cells. The cells infected by AcPolhEKA-EGFP produced fluorescent granules. The EGFP fusion protein was purified from granule-containing cells in three steps: cell harvest, sonication, and EK digestion. Through final enterokinase digestion, EGFP presented mainly in the supernatant, and this supernatant fraction also showed a pure EGFP band. These results suggest that a combined procedure of Polh fusion expression and enterokinase digestion can be used for rapid and easy purification of other proteins.</P>