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Byun, Kyung Min,Jang, Seong Min,Kim, Sung June,Kim, Donghyun The Optical Society 2009 Journal of the Optical Society of America. A, Opti Vol.26 No.4
<P>A localized surface plasmon resonance (LSPR) biosensor using surface relief nanostructures was investigated to evaluate the importance of target localization on the sensitivity enhancement. The LSPR device was modeled as periodic metallic nanowires with a square profile on a gold film and the target as a self-assembled monolayer in buffer solution. The numerical results using rigorous coupled-wave analysis and the finite-difference time domain method demonstrated localized plasmonic fields induced by the surface nanostructure from which the effect of target localization on the sensitivity was quantitatively analyzed. Interestingly, it was found that target localization on nanowire sidewalls improves sensitivity significantly because of strong overlap with localized plasmonic fields. An LSPR structure optimized for a localized target on sidewalls provides sensitivity enhancement per unit target volume by more than 20 times in water ambience.</P>
Effects of ice-binding protein from Leucosporidium on the cryopreservation of boar sperm
Sang Hyoun Park,Keon Bong Oh,Sun-A Ock,Sung June Byun,Hwi-Cheul Lee,Suresh Kumar,Sung Gu Lee,Jae-Seok Woo 한국수정란이식학회 2018 한국동물생명공학회지 Vol.33 No.3
The aim of this study was performed to evaluate the effects of ice-binding protein from the arctic yeast Leucosporidium (LeIBP) supplementation on cryopreservation of boar sperm. The collected semen was diluted (1.5×108/ml) in lactose egg yolk (LEY) and cooled at 5°C for 3 h. The cooled semen was then diluted (1×108/ml) in LeIBP containing LEY with 9% glycerol and maintained at 5°C for 30 min. The semen was divided into six experimental groups (control, 0.001, 0.005, 0.01, 0.05 and 0.1 mg/ml of LeIBP). The straws were kept on above the liquid nitrogen (LN2) vapors for 20 minutes and then plunged into LN2. After thawing, computer-assisted sperm analysis was used for sperm motility and flow cytometry was performed to assess the viability, acrosome integrity (FITC-PSA/PI), ROS (DCF/PI), lipid peroxidation (BODIPY C11/PI) and apoptosis (Annexin V/PI), respectively. No significant responses were observed for sperm motility. However, sperm viability was significantly increased on 0.05 and 0.1 mg/ml of LeIBP groups compared to control (P < 0.05). In addition, acrosome integrity was significantly increases LeIBP groups (P < 0.05) and both ROS and lipid peroxidation level were lower in all LeIBP groups than those of control (P < 0.05). On the other hand, a significant higher apoptosis rate was observed in 0.05 and 0.1 mg/ml of LeIBP groups compared to control (P < 0.05). It can be assumed that a supplementation of LeIBP in boar sperm freezing extender is an effective method to increase the sperm qualities after cryopreservation.
Effects of Leucosporidium Ice-binding Protein on the Cryopreservation of Boar Sperm
Sang Hyoun Park,Keon Bong Oh,Sun-A Ock,Sung June Byun,Hwi-Cheul Lee,Hyeon Yang,Seongsoo Hwang,Gi-Sun Im,Jae-Seok Woo 한국동물생명공학회(구 한국동물번식학회) 2018 발생공학 국제심포지엄 및 학술대회 Vol.2018 No.06
This study was performed to evaluate effects of Leucosporidium ice-binding protein (LeIBP) supplementation on boar (Duroc) sperm freezing. The collected semen was diluted (1.5×10⁸ /mL) in lactose egg yolk (LEY) and cooled at 5°C for 3 h. The cooled semen was then diluted (1×10⁸ /mL) in LeIBP containing LEY with 9% glycerol and maintained at 5°C for 30 min. The semen was divided into six experimental groups (control, 0.001, 0.005, 0.01, 0.05, and 0.1 mg/mL of LeIBP). The straws were stayed above the liquid nitrogen (LN2) vapors for 20 minutes and then plunged into LN2. After thawing, sperm parameters such as motility, viability, and acrosome integrity, intracellular reactive oxygen species (ROS), apoptosis, and undetected lipid peroxidation were determined. Computer assisted sperm analysis was used for sperm parameters and flowcytometry was performed to acrosome integrity (FITC-PSA/PI), ROS (DCFHDA/ PI), lipid peroxidation (BODIPY C11/PI), and apoptosis (Annexin V/PI), respectively. The differences were not in sperm motility, but in viability of 0.05 and 0.1 mg/mL groups compared to control (p<0.05). Higher acrosome integrity was observed in LeIBP groups compared to control (p<0.05), except for 0.001 group. Both ROS and lipid peroxidation level were lower in all LeIBP groups than that of control (p<0.05). Furthermore, lower apoptosis rate was observed in both 0.005 and 0.1 LeIBP groups compared to control (p<0.05). It can be postulated that a supplementation of LeIBP in boar sperm freezing extender is an effective method to increase the sperm qualities after cryopreservation.
DNA barcoding of the 76 species of family Crambidae (Lepidoptera) in Korea
June-Hyeok Jeong,Ji-Young Lee,Jae-In Oh,Sang-Yoon Kim,Jun-Min Seo,Bong-Woo Lee,Il-Kwon Kim,Bong-Kyu Byun 한국응용곤충학회 2023 한국응용곤충학회 학술대회논문집 Vol.2023 No.10
The family Crambidae, so called as Grass moths, have been known as a difficult group in identification due to their similar wing patterns among the allied species. Some species are well known as pests to the agricultural and ornamental plants of economic importance. Therefore, this study was carried out to provide the DNA barcode and related information for distinguishing the complex species among Crambidae. In this study, we extracted and analyzed the DNA barcodes from 185 specimens of 76 species in the family Crambidae. Based on the result, one species of the genus Schoenobius, Pagyda, Neoanalthes, Palpita, Ecpyrrhorrhoe and Paratalanta area reported for the first time from Korea.
The establishment of KORCC (KOrean Renal Cell Carcinoma) database
Byun, Seok-Soo,Hong, Sung Kyu,Lee, Sangchul,Kook, Ha Rim,Lee, Eunsik,Kim, Hyeon Hoe,Kwak, Cheol,Ku, Ja Hyeon,Jeong, Chang Wook,Lee, Ji Youl,Hong, Sung Hoo,Kim, Yong-June,Hwang, Eu Chang,Kwon, Tae Gyun The Korean Urological Association 2016 Investigative and Clinical Urology Vol.48 No.6
<P><B>Purpose</B></P><P>The purpose of this article is to report establishment of the 1st Web-based database (DB) system to collect renal cell carcinoma (RCC) data in Korea.</P><P><B>Materials and Methods</B></P><P>The new Web-based DB system was established to collect basic demographic and clinicopahtological characteristics of a large cohort of patients with RCC in Korea. Data from a total of 6,849 patients were collected from 8 tertiary care hospitals that agreed to participate in organizing the Korean Renal Cell Carcinoma (KORCC) study group as of 1 July 2015. Basic demographic and clinicopathological characteristics were collected. The data of patients who underwent surgical treatments were analyzed to characterize Korean RCC.</P><P><B>Results</B></P><P>We established the 1st Web-based DB of Korean RCC, a database comprising renal mass management cases from multiple centers in Korea. The data of 5,281 patients who underwent surgical management (mean follow-up, 32 months) were analyzed. The most common symptom was incidentally detected renal mass (76.9%). Clinical T1a was the most common (54.3%) stage and mean tumor size was 4.8±4.2 cm. Radical nephrectomy accounted for 62.7% of cases and an open approach was used in 50.7% and 52.2% of radical and partial nephrectomies, respectively. The 5-year overall, cancer-specific and recurrence-free survival rates were 88.1%, 92.2%, and 88.0%, respectively.</P><P><B>Conclusions</B></P><P>We report the 1st establishment of a Web-based DB system to collect RCC data in Korea. This DB system will provide a solid basis for the characterization of Korean RCC.</P>
Byun, Seok-Soo,Hwang, Eu Chang,Kang, Seok Ho,Hong, Sung-Hoo,Chung, Jinsoo,Kwon, Tae Gyun,Kim, Hyeon Hoe,Kwak, Cheol,Kim, Yong-June,Lee, Won Ki Hindawi Publishing Corporation 2016 BioMed research international Vol.2016 No.-
<P><I>Background</I>. The prognostic significance of the neutrophil-to-lymphocyte ratio (NLR) in nonmetastatic renal cell carcinoma (non-mRCC) is controversial, although NLR has been established as a prognostic factor in several cancers. The objective of our study was to assess the prognostic significance of preoperative NLR in non-mRCC, based on a large, multicenter cohort analysis.<I> Methods</I>. Totally, 1,284 non-mRCC patients undergoing surgery were enrolled from six institutions between 2000 and 2014. Recurrence-free survival (RFS) and cancer-specific survival (CSS) were calculated, and the prognostic significance of NLR was evaluated.<I> Results</I>. Patients with higher NLR had larger tumors (<I>p</I> < 0.001), higher T stage (<I>p</I> < 0.001), worse Eastern Cooperative Oncology Group performance status (<I>p</I> < 0.001), worse symptoms (<I>p</I> = 0.003), sarcomatoid differentiation (<I>p</I> = 0.004), and tumor necrosis (<I>p</I> < 0.001). The 5-year RFS and CSS rates were significantly lower in patients with high NLR than in those with low NLR (each <I>p</I> < 0.001). Multivariate analysis identified NLR to be an independent predictor of RFS and CSS (each <I>p</I> < 0.05). Moreover, predictive accuracy of multivariate models for RFS and CSS increased by 2.2% and 4.2%, respectively, with NLR inclusion.<I> Conclusions</I>. Higher NLR was associated with worse clinical behavior of non-mRCC. Also, NLR was a significant prognostic factor of both RFS and CSS.</P>
Human extracellular superoxide dismutase (EC-SOD) expression in transgenic chicken
Sung June Byun,Mi Ran Ji,Ye Jin Jang,A In Hwang,Hee Kyoung Chung,Jeom Sun Kim,Kyung Woon Kim,Hak Jae Chung,Byoung Chul Yang,Ik Soo Jeon,Jin Ki Park,Jae Gyu Yoo,Tae Yoon Kim 생화학분자생물학회(구 한국생화학분자생물학회) 2013 BMB Reports Vol.46 No.8