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RISS 인기검색어
- 검색키워드
- 저자 : Byeong Geun Gwon (검색결과 16 건)
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Byeong Geun Gwon,Joong Kyun Kim 대한환경공학회 2012 Environmental Engineering Research Vol.17 No.1
A scaled-up bioconversion of fishmeal wastewater (FMW) into liquid fertilizer was performed five times in a 1 m3 reactor in order to examine the feasibility of commercialization. The importance of aeration was marked. Analyses indicated that dissolved oxygen (DO) level was closely related to the value of oxidation-reduction potential (ORP) and it was crucial to achieve high-quality liquid fertilizer. When pure oxygen was supplied through four diffusers into the reactor, DO levels and ORP values were maintained over 1.2 mg/L and 0.2 mV, respectively all the time during 52 hr of bioconversion. The pH changed from 6.8 to 5.9. The average removal percentages of chemical oxygen demand (CODCr) and total nitrogen (TN) were 75.0% and 71.6%, respectively. Compared to the result acquired in a 5-L reactor, bioconversion of FMW into liquid fertilizer was achieved in a shorter time under the same removal percentages of CODCr and TN. The 52-hr culture of inoculated FMW was phytotoxic-free and it possessed comparable fertilizing ability to a liquid fertilizer made from the fish waste in hydroponic culture with amino acid contents of 5.93 g/ 100 g sample. From all the above results, transferring labscale data to large-scale production appeared to be successful. As a result, the commercialization of a liquid fertilizer made from FMW was feasible.
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Gwon, Byeong-Geun,Kim, Joong-Kyun Korean Society of Environmental Engineers 2012 Environmental Engineering Research Vol.17 No.1
A scaled-up bioconversion of fishmeal wastewater (FMW) into liquid fertilizer was performed five times in a $1m^3$ reactor in order to examine the feasibility of commercialization. The importance of aeration was marked. Analyses indicated that dissolved oxygen (DO) level was closely related to the value of oxidation-reduction potential (ORP) and it was crucial to achieve high-quality liquid fertilizer. When pure oxygen was supplied through four diffusers into the reactor, DO levels and ORP values were maintained over 1.2 mg/L and 0.2 mV, respectively all the time during 52 hr of bioconversion. The pH changed from 6.8 to 5.9. The average removal percentages of chemical oxygen demand ($COD_{Cr}$) and total nitrogen (TN) were 75.0% and 71.6%, respectively. Compared to the result acquired in a 5-L reactor, bioconversion of FMW into liquid fertilizer was achieved in a shorter time under the same removal percentages of $COD_{Cr}$ and TN. The 52-hr culture of inoculated FMW was phytotoxic-free and it possessed comparable fertilizing ability to a liquid fertilizer made from the fish waste in hydroponic culture with amino acid contents of 5.93 g/ 100 g sample. From all the above results, transferring lab-scale data to large-scale production appeared to be successful. As a result, the commercialization of a liquid fertilizer made from FMW was feasible.
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권병근 ( Byeong Geun Gwon ),김영옥 ( Young Ok Kim ),남보혜 ( Bo Hye Nam ),김우진 ( Woo Jin Kim ),공희정 ( Hee Jeong Kong ),김봉석 ( Bong Seok Kim ),지영주 ( Young Ju Jee ),이상준 ( Sang Jun Lee ),안철민 ( Cheul Min An ),김동균 ( D 한국어병학회 2013 한국어병학회지 Vol.26 No.3
The ark shell, Scapharca broughtonii is a marine bivalve mollusks belonging to the family Arcidae and important seafood for Korean and Japanese, and southern coast is brisk bays for the ark shell aquaculture. However, productivity of ark shell from these regions were rapidly reduced during the last decade due to mass mortality. The reason of this great damage has not yet been identified. To overcome this economic loss, diverse investigations were focused on environmental factors that affects in the physiology of S. broughtonii, but microbiological researches were performed insufficiently. Hemoglobin is one of the major blood component of ark shell and is damaged by some species of bacterial toxins. We concentrated on this red pigment because hemolysis could be the cause of ark shell mortality. In this study, we analyzed microbial diversity of underwater sediments in coastal regions and also existences in the body of S. broughtonii. We investigate about 4,200 isolates collected from June to September for microbial diversity of sediments and ark shell. We screened all of culturable microorganisms, and identified 25 genera 118 species, 24 genera 89 species, 30 genera 109 species and 39 genera 141 species, and selected 140 unique colonies for identification and challenge assay.
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허혈-재관류 신손상 백서에서 Cyclosporin A와 Mycophenolate Mofetil이 TGF-β1 및 Endothelin-1의 발현에 미치는 영향
한병근 ( Han Byeong Geun ),송기학 ( Song Gi Hag ),이재명 ( Lee Jae Myeong ),양재원 ( Yang Jae Won ),최정욱 ( Choe Jeong Ug ),김정권 ( Kim Jeong Gwon ),최승옥 ( Choe Seung Og ) 대한신장학회 2003 Kidney Research and Clinical Practice Vol.22 No.3
목 적 : 현재 가장 널리 사용되는 면역억제제 중의 하나인 Cyclosporin A (CsA)가 신이식의 성적을 향상시켰으나 신독성으로 인해 이식신의 장기적인 생존율에는 영향을 미치지 못하고 있다. CsA에 의해 생성이 증가된 Transforming growth factor-β (TGF-β)와 Endothelin-1 (ET-1)이 주요한 신독성의 매개인자로 밝혀졌다. 최근 Azathioprine 대용으로 Mycophenolate mofetil (MMF)이 널리 사용되어 CsA와 MMF를 병합투여 시 면역억제기능이 상승하지만 CsA의 신독성을 매개하는 인자에 대한 연구는 없다. 이에 본 연구에서는 허혈-재관류 모델 백서에서 CsA와 MMF를 단독 또는 병합투여 시 CsA 신독성의 중요한 매개인자인 TGF-β1과 ET-1의 생성과 발현에 어떤 영향을 미치는지 알아보고자 하였다. 방 법 : 단일 신장 백서의 혈관경 (renal pedicle)을 30분간 클램프한 후 재관류하여 허혈-재관류 손상을 유발시켜 대조군, 허혈-재관류군, CsA 투여군, CsA와 MMF 병합투여군, MMF 투여군으로 나누어 매개물질의 합성 및 발현을 면역조직화학염색, 역전사 중합효소연쇄반응 및 공초점레이져주사현미경 (confocal microscope)으로 측정하였다. 결 과 : CsA를 사용한 군에서 세뇨관의 공포 형성과 같은 조직학적 손상이 나타나 CsA에 의한 신독성을 간접적으로 확인할 수 있었다. TGF-β1은 주로 세뇨관에서 발현이 되고 ET-1은 혈관의 내피세포와 보우만씨 소체에서 주로 발현되었다. TGF-β1의 발현은 CsA 투여군에서 면역조직화학염색과 공초점레이져주사현미경 검사상 대조군과 다른 실험군에 비해 증가되었다. ET-1의 발현은 CsA 투여군에서 면역조직화학염색, mRNA 및 공초점레이져주사현미경 검사상 대조군과 다른 실험군에 비해 발현이 증가되었다. TGF-β1과 ET-1의 발현은 병합요법군이 CsA 투여군보다 감소되었다. MMF 투여군에서의 TGF-β1과 ET-1의 발현은 허혈-재관류군과 비슷한 양상을 보였으며, CsA군과 병합요법군보다 낮은 발현율을 보였다. 결 론 : 허혈-재관류 신손상 시 MMF는 CsA와는 다르게 TGF-β1이나 ET-1의 발현을 증가시키지 않으며 특히 CsA와 병합투여 시 CsA 단독투여 시 보다 유의하게 TGF-β1과 ET-1의 발현을 감소시키는 것으로 보아 CsA와 MMF의 병합요법은 장기이식 환자에서 CsA 치료의 아킬레스건인 신독성을 줄일 수 있을 것으로 생각된다. Purpose: Nephrotoxicity of cyclosporin A (CsA) remains a major obstacle for the clinical use of this potent immunosuppressant. It is likely that the transforming growth factor-β (TGF-β) and endothelin-1 (ET-1)play a central role in initiation and/or progression of CsA induced nephropathy in renal is-chemia-reperfusion injury. It is proposed that the new immunosuppressive drug mycophenolate mofetil (MMF) reduces the incidence of acute rejection in comparison with azathioprine. Thus, the aim of the present study was to analyze the wxpression of TGF-β and ET-1 in renal ischemia-reperfusion in jured rats and to see the effect of CsA or MMF on the expression of these mediators. Effects of coad ministration of CsA and MMF were also evaluated. Methods: Sprague-Dawley rats (N=60) performed right nephrectomy were classified into five groups according to experimental methods. Control group underwent right nephrectomy. After the right nephrectomy, to induce renal ischemia, the left renal vascular pecicle was occluded for 30 minutes with vascular pedicle was occluded for 30 minutes with vascular clamps in all experimental groups. After 30 minutes, the clamps were removed to undergo reperfusion. In control groupl. ischemic injury wasn`t done. CsA group was administered CsA (10mg/kg/day, S.C) after the operation. CsA and MMF group was coadministered CsA (10mg/kg/day, S.C) and MMF (10mg/kg/day, P.O). MMF group was administered MMF (10mg/kg/day, P.O). After 7 days, the left kidney was removed and processed for histological, immunohistochernical, immunofluorescent and molecular analyses for TGF-β1 and histological, immunohistochemical, immunofluorescent analyses for ET-1. Results: The immunohistochemical and immunofluorescent expression density for TGF-β-1 in CsA group was higher than control and other experimental groups. The immunohistochemical and immunofluorescent expression density and mRNA production for ET-1 in CsA group was higher than control and other experimental groups. The expression density for TGF-β-1 and ET-1 CsA group. The expression density for TGF-β-1 and ET-1 in MMF group was less than in CsA group and CsA and MMF group, was similar to ischemia-reperfusion group. Conclusion: These results suggest that MMF does not seem to have effect from CsA in seems to have a reno-protective effect from CsA induced nephrotoxicity in the ischemia-reperfusion model. The present study might partially explain that MMF is going to contribute to the improvement of the survival rate of the transplanted kidney associated with acute and/or chronic rejection and to the reduction of CsA dosage and its complications. (Korean J Nephrol 2003;22(3):273-284)
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Vacuum Packaging Technology of AC-PDP using Direct-Joint Method
Lee, Duck-Jung,Lee, Yun-Hi,Moon, Gwon-Jin,Kim, Jun-Dong,Choi, Won-Do,Lee, Sang-Geun,Jang, Jin,Ju, Byeong-Kwon The Korean Infomation Display Society 2001 Journal of information display Vol.2 No.4
We suggested new PDP packaging technology using the direct joint method, which does not need an exhausting hole and tube. The advantages of this method are simple process, short process time and time panel package. To packaging, we drew the seal line of glass frit by dispenser followed by forming the lump, which provide pumping-out path during the packaging process. And, we have performed a pretreatment of glass frit to reduce the out-gases. After which, both front and rear glass plates were aligned and loaded into vacuum packaging chamber. The 4-inch monochrome AC-PDP was successfully packaged and fully emitted with brightness of 1000 $cd/m^2$. Also, glass frit properties for pretreatment condition was investigated by AES and SEM analyses.
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