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      • SCOPUSKCI등재
      • 全南地方의 生乳와 市乳의 品質 및 衛生的 管理에 對한 調査硏究

        林鳳鎬,趙成萬,金宇權,李載洪,崔洙亨,朴南鏞 全南大學校 農漁村開發硏究所 1976 農業科學技術硏究 Vol.11 No.-

        A survey of sanitary and qualitative control of raw and market milk in Chonnam district was carried out in quest of the grade of propriety by the legal standards. For this study 149 raw milk samples, which were taken from the pastures in Chonnarn district, were collected from the milk plants and l61 market milk samples were collected from the shops in Gwang-3u city- In order to survey the status of control of raw milk in every pasture, the pastures were divided into three groups. A group was the pasture raising more than 20 cows; B group, 10 ~ 20 cows; C group, less 10 cows. The results obtained were as follows: 1. The mean value of specific gravity was 1.029, acidity was 0.14 per cent and fat content was 3.2 per cent in i49 raw milk samples, and 1.029, 0.13 per cent and 3. 1 per cent in 161 market milk respectively. The passing rates by legal standards of specific gravity, acidity and fat content in raw milk were 78.7 per cent, 96.7 per cent and 75.3 per cent, and in market milk 66.5 per cent, 97.5 per cent and 66.5 per cent respectively. The fact that except for acidity the passing rates of market milk in specific gravity and fat content were less than those of raw milk indicated unfit processing for market milk by the milk plants. 2. The acceptable level for bacterial numbers in raw milk by legal standard were 65.1 per cent by standard plate count and 69.8 per cent by direct microscopic count. The acceptable level by legal standards for bacterial number in raw milk among the pastures were 62.5 per cent in A group, 65.2 per cent in B group, 64.8 per cent in C group by standard plate count, and 68.8 per cent, 71.7 per cent and 71.8 per cent by direct microscopic count respectively. As mentioned above the sanitary and qualitative control and treatment of raw and market milk were yet unfit to date in Chonnam district, but it shall be improved gradually in the future.

      • Mycobacterium scrofulaceum에 의한 마우스 폐의 Superoxide Dismutase유도

        이봉진,정상인,최철순,양용태 중앙대학교 간호과학연구소 1991 中央醫大誌 Vol.16 No.3

        This study was undertaken to investigate whether the induction of superoxide dismutase in the lung was affected by infection with M. scrofulaceum. In addition, association between the plasmidbearing and induction of superoxide dismutase(SOD) were investigated with various strains of M. scrofulaceum. When M. scrofulaceum were injected to ICR-JCL mouse intravenously, type 42 CDC 1198 and type 43 Brooks strains induced superoxide dismutase in the lungs, which no significant changes in the activity of superoxide dismutase in the spleen were observed. Strains of M. scrofulaceum which possessed a large plasmid(>80 Kb)induced much oxygen metabolites in the host than the plasmidless strains. This result suggests that more virulent strains of M. scrofulaceum induces the activity of superoxide dismutase in the host to protect the injuries from the reactive oxygen species during the infection.

      • KCI등재

        고인성 라인파이프강의 인성과 천이온도의 분석 Ⅲ. Charpy 충격특성, DWTT, CTOA의 상관관계

        황병철,신상용,이성학,김낙준,강기봉 대한금속재료학회 2004 대한금속·재료학회지 Vol.42 No.9

        Correlation of Charpy V-notch (CVN) impact properties, drop-weight tear test (DWTT) properties, and crack tip opening angle (CTOA) values of a high-toughness API X70 pipeline steel was investigated in this study. Two-specimen CTOA test (TSCT) was conducted on the rolled steel specimens to measure CTOA, and the test results were related with the CVN and DWTT data to find correlations between them. The CVN total energy density showed almost 1:1 linear correlation with the DWTT initiation energy density. The TSCT test results indicated that the specimens rolled in the single phase region had the larger CTOA as well as the higher CVN and DWTT energies than those rolled in the two phase region because their microstructures were composed of acicular ferrites and fine polygonal ferrites. The measured CTOA showed better correlation with the DWTT propagation energy than with the CVN or DWTT total energy as it was related with the crack propagation speed. Since the value of Sin(2CTOA) was linearly proportional to the DWTT energy, it could be used as a useful parameter to reliably estimate the CTOA.

      • 주식 투자 전문가 시스템에 관한 구현 연구

        한정현,이성호,강훈규,양봉진,이일병 연세대학교 자연과학연구소 1989 學術論文集 Vol.23 No.-

        본 논문은 주식투자를 문제영역으로 하는 전문가 시스템 설계와 구현 연구에 관한 것이다. 주식투자와 관련된 기존 프로그램들의 대부분이 데이타베이스, 통계분석, 시장모델 등에 관한 것이었으나 본 논문에서 구현하는 주식투자 전문가 시스템인 ASIE(Artificial Stock Investment Expert) 시스템은 주식투자 분야의 투자 전문지식을 조직화해서 주식투자 전문가 시스템을 구축하고, 이를 사용자들에게 제공하여 효율적이고 합리적인 투자를 할 수 있도록 도와주는 것을 목표로 한다. ASIE 시스템은 현재의 상황에 대한 사용자의 의견을 입력으로 받아 지식베이스에 들어 있는, 투자에 경험이 많은 전문인의 경험적 지식으로 추론을 하여 여러가지 투자 조건에 알맞는 적절한 투자 종목을 선정해 준다. Design and implementation of an expert system for stock investment is described. Existing programs related to stock investments are mostly about database management, statistical analysis systems or mathematical model of stock markets, instead the ASIE(Artificial Stock Investment Expert)system described is an expert system, which systematizes human expert knowledges for stock investment and provides inferred decision alternatives to users for better investment decisions. The ASIE system incorporates the user's opinions about current situations and performs inferences using the formalized knowledges in knowledge-base together with other data in the database and finally composes the preferred portfolio suitable for the various investment conditions and user's personal preferences.

      • Ultrasonic isolation of the outer membrane of Escherichia coli with autodisplayed Z-domains

        Bong, J.H.,Yoo, G.,Park, M.,Kang, M.J.,Jose, J.,Pyun, J.C. IPC Science and Technology Press ; Elsevier Scienc 2014 Enzyme and microbial technology Vol.66 No.-

        The outer membrane of Escherichia coli was previously isolated as a liposome-like outer membrane particle using an enzymatic treatment for lysozymes; for immunoassays, the particles were subsequently layered on solid supports via hydrophobic interactions. This work presents an enzyme-free isolation method for the E. coli outer membrane with autodisplayed Z-domains using ultrasonication. First, the properties of the outer membrane particle, such as the particle size, zeta potential, and total protein, were compared with the properties of particles obtained using the previous preparation methods. Compared with the conventional isolation method using an enzyme treatment, the ultrasonic method exhibited a higher efficiency at isolating the outer membrane and less contamination by cytosolic proteins. The isolated outer membrane particles were layered on a gold surface, and the roughness and thickness of the layered outer membrane layers were subsequently analyzed using AFM analysis. Finally, the antibody-binding activity of two outer membrane layers with autodisplayed Z-domains created from particles that were isolated using the enzymatic and ultrasonic isolation methods was measured using fluorescein-labeled antibody as a model analyte, and the activity of the outer membrane layer that was isolated from the ultrasonic method was estimated to be more than 20% higher than that from the conventional enzymatic method.

      • SCIESCOPUSKCI등재

        Sequencing of cDNA Clones Expressed in Adipose Tissues of Korean Cattle

        Bong, J.J.,Tong, K.,Cho, K.K.,Baik, M.G. Asian Australasian Association of Animal Productio 2005 Animal Bioscience Vol.18 No.4

        To understand the molecular mechanisms that regulate intramuscular fat deposition and its release, cDNA clones expressed in adipose tissues of Korean cattle were identified by differential screening from adipose tissue cDNA library. By partial nucleotide sequencing of 486 clones and a search for sequence similarity in NCBI nucleotide databases, 245 clones revealed unique clones. By a functional grouping of the clones, 14% of the clones were categorized to metabolism and enzyme-related group (stearoyl CoA desaturase, lactate dehydrogenase, fatty acid synthase, ATP citrate lyase, lipoprotein lipase, acetyl CoA synthetase, etc), and 6% to signal transduction/cell cycle-related group (C/EBP, cAMP-regulated phosphoprotein, calmodulin, cyclin G1, cyclin H, etc), and 4% to cytoskeleton and extracellular matrix components (vimentin, ankyrin 2, gelosin, syntenin, talin, prefoldin 5). The obtained 245 clones will be useful to study lipid metabolism and signal transduction pathway in adipose tissues and to study obesity in human. Some clones were subjected to full-sequencing containing open reading frame. The cDNA clone of bovine homolog of human prefoldin 5 gene had a total length of 959 nucleotides coding for 139 amino acids. Comparison of the deduced amino acid sequences of bovine prefoldin 5 with those of human and mouse showed over 95% identity. The cDNA clone of bovine homolog of human ubiquitin-like/S30 ribosomal fusion protein gene had a total length of 484 nucleotides coding for 133 amino acids. Comparison of the deduced amino acid sequences of bovine ubiquitin-like/S30 ribosomal fusion protein gene with those of human, rat and mouse showed over 97% identity. The cDNA clone of bovine homolog of human proteolipid protein 2 mRNA had a total length of 928 nucleotides coding for 152 amino acids. Comparison of the deduced amino acid sequences of bovine proteolipid protein 2 with those of human and mouse showed 87.5% similarity. The cDNA clone of bovine homolog of rat thymosin beta 4 had a total length of 602 nucleotides coding for 44 amino acids. Comparison of the deduced amino acid sequences of bovine thymosin beta 4 gene with those of human, mouse and rat showed 93.1% similarity. The cDNA clone of bovine homolog of human myotrophin mRNA had a total length of 790 nucleotides coding for 118 amino acids. Comparison of the deduced amino acid sequences of bovine myotrophin gene with those of human, mouse and rat showed 83.9% similarity. The functional role of these clones in adipose tissues needs to be established.

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