http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Role of Selenium in Alteration of Erythrocyte Parameters in Bovine Fluorosis
Han, Bo,Yoon, Soon-Seek,Wu, Pei-Fu,Han, Hong-Ryul,Liang, Li-Cheng Asian Australasian Association of Animal Productio 2006 Animal Bioscience Vol.19 No.6
Signs of dental discolouration, difficulty in mastication, bony exostosis and debility were observed in cattle from Qingtongxia Ningxia, China where fluoride concentration in drinking water, soil, fodder, serum, bone, teeth, haircoat and urine were significantly higher than the corresponding health site. The problem of fluorosis in beef cattle is attributable to water containing toxic levels of fluoride. The objective of this paper was therefore to evaluate the influence of fluoride on erythrocyte parameters in cattle under high fluoride and low selenium conditions, as well as the protective efficacy of selenium exposure in feedstuff for bovine endemic fluorosis. Sixteen 6 to 7 year-old high fluoride beef cattle were randomly allotted into two groups each with eight cows: high fluoride control group, and supplemented with 0.25 mg/kg selenium per day for 83 days respectively. In addition, eight 6 to 7 year-old normal control beef cattle were selected from a non-high fluoride site. Blood samples were collected on day 0, 30 and 83 for erythrocyte parameters analysis and scanning electronic microscopy. The results indicated that erythrocytes, hemoglobin, packed cell volume values and $Na^+-K^+$ ATPase activity from affected cattle on the high fluoride site were significantly reduced during the period as compared with the corresponding samples of normal control cattle, a great number of echinocytes were present in peripheral blood, and subsequent anaemia. However, affected cattle exposed to selenium revealed increasable erythrocyte parameters, the extent of elevation in these values being dependent on the duration of supplementation with selenium. These findings suggest that fluoride exposure can cause erythrocyte damage, whereas selenium supplementation can antagonize fluoride-induced generation of free radicals and cumulative effects of lipid peroxidation in erythrocytes. Selenium supplementation may help to alleviate the possible hazards associated with bovine endemic fluorosis.
( Bo Xu ),( Li Ming Dai ),( Jun Jun Li ),( Meng Deng ),( Hua Biao Miao ),( Jun Pei Zhou ),( Yue Lin Mu ),( Qian Wu ),( Xiang Hua Tang ),( Yun Juan Yang ),( Jun Mei Ding ),( Nan Yu Han ),( Zun Xi Huang 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.1
Xylanases sourced from different bacteria have significantly different enzymatic properties. Therefore, studying xylanases from different bacteria is important to their applications in different fields. A potential xylanase degradation gene in Massilia was recently discovered through genomic sequencing. However, its xylanase activity remains unexplored. This paper is the first to report a xylanase (XynRBM26) belonging to the glycosyl hydrolase family (GH10) from the genus Massilia. The gene encodes a 383-residue polypeptide (XynRBM26) with the highest identity of 62% with the endoxylanase from uncultured bacterium BLR13. The XynRBM26 expressed in Escherichia coli BL21 is a monomer with a molecular mass of 45.0 kDa. According to enzymatic characteristic analysis, pH 5.5 is the most appropriate for XynRBM26, which could maintain more than 90% activity between pH 5.0 and 8.0. Moreover, XynRBM26 is stable at 37°C and could maintain at least 96% activity after being placed at 37°C for 1 h. This paper is the first to report that GH10 xylanase in an animal gastrointestinal tract (GIT) has salt tolerance, which could maintain 86% activity in 5 M NaCl. Under the optimum conditions, Km, Vmax, and kcat of XynRBM26 to beechwood xylan are 9.49 mg/ml, 65.79 μmol/min/mg, and 47.34 /sec, respectively. Considering that XynRBM26 comes from an animal GIT, this xylanase has potential application in feedstuff. Moreover, XynRBM26 is applicable to high-salt food and seafood processing, as well as other high-salt environmental biotechnological fields, because of its high catalytic activity in high-concentration NaCl.
한성호,김치홍,안태원,양보규,주우준 대한골절학회 1997 대한골절학회지 Vol.10 No.3
Pilon fracture is relatively an uncommon fracture involving the distal tibial articular surface. As usually being combined with many complications, it is difficult to manage. Among many treatment options limited internal fixation of the tibia with long screws and multiple pins augmented with external fixation or casting provide adequate stabilization without soft tissue compromise, Fractures were defined as type II in 10 fractures and type III in 16 by Ruedi-Allgower's classification. 16 fractures. 6 type II and 10 type III, had limited internal fixation and cast application. 1 type I fracture had limited internal and external fixation. 9 fractures, 4 type I and 5 type I, were treated by rigid tibial plating during a period of 5 years (Mar. 1990 - Fed. 1995). By Burwell's and Charnley' s radiological criteria and clinical grading system, limited internal fixation showed 67% satisfactory results in type I and 64% : in type I fractures while rigid tibial plating showed 75% satisfactory results in type 3 and 60% in type I fractures. 4(44%) patients with rigid tibial plating, and 2(12%) patients with limited internal fixation had complications. Pilon fractures are high energy injuries with significant associated soft tissue damage. limited internal fixation offers good solution to this difficult fracture problem.
Nanowire Mechanical Switch with a Built-In Diode
Han, Jin-Woo,Ahn, Jae-Hyuk,Kim, Min-Wu,Lee, Jeong Oen,Yoon, Jun-Bo,Choi, Yang-Kyu WILEY-VCH Verlag 2010 Small Vol.6 No.11
<B>Graphic Abstract</B> <P>A nanoelectromechanical switch consists of two landing electrodes on either side of a movable silicon nanowire, separated by a nanoscale air gap. The bistable mechanical state of the nanowire determines an on/off state, which is sensed by measuring the contact resistance. The n-type semiconductor electrodes and p-type nanowire form a p–n junction diode at the cross-point. <img src='wiley_img_2010/16136810-2010-6-11-SMLL201000170-content.gif' alt='wiley_img_2010/16136810-2010-6-11-SMLL201000170-content'> </P>
Fabrication of 3D nano-structures by two photon absorption polymerization
Bo Young Kang,Song-Hee Han,Bum Ku Rhee,Jeong Weon Wu,Kwang-Sup Lee 한국물리학회 2004 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.45 No.2
A two-photon absorption polymerization process is employed to fabricate 3D nano-structures. Resins transparent at 800 nm but highly absorbing at 400 nm undergo a two-photon absorption and polymerization upon irradiation with fundamental light from a Ti:Sapphire femtosecond laser. By use of the re ection imaging technique to identify the position of the focal point, a series of slanted nano-structures and a 3D layered-structure are successfully fabricated.
Application of advanced biomaterials in photothermal therapy for malignant bone tumors
Bo Chao,Jianhang Jiao,Lili Yang,Yang Wang,Weibo Jiang,Tong Yu,Linfeng Wang,He Liu,Han Zhang,Zhonghan Wang,Minfei Wu 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00
Malignant bone tumors are characterized by severe disability rate, mortality rate, and heavy recurrence rate owing to the complex pathogenesis and insidious disease progression, which seriously affect the terminal quality of patients' lives. Photothermal therapy (PTT) has emerged as an attractive adjunctive treatment offering prominent hyperthermal therapeutic effects to enhance the effectiveness of surgical treatment and avoid recurrence. Simultaneously, various advanced biomaterials with photothermal capacity are currently created to address malignant bone tumors, performing distinctive biological functions, including nanomaterials, bioceramics (BC), polymers, and hydrogels et al. Furthermore, PTT-related combination therapeutic strategies can provide more significant curative benefits by reducing drug toxicity, improving tumor-killing efficiency, stimulating anti-cancer immunity, and improving immune sensitivity relative to monotherapy, even in complex tumor microenvironments (TME). This review summarizes the current advanced biomaterials applicable in PTT and relevant combination therapies on malignant bone tumors for the first time. The multiple choices of advanced biomaterials, treatment methods, and new prospects for future research in treating malignant bone tumors with PTT are generalized to provide guidance.
Isolation, Culture and Identification of Porcine Skeletal Muscle Satellite Cells
Bo-jiang Li,Ping-hua Li,Rui=hua Huang,Wen-xing Sun,Han Wang,Qi-fa Li,Jie Chen,Wang Jun Wu,Honglin Liu 아세아·태평양축산학회 2015 Animal Bioscience Vol.28 No.8
The objective of this study was to establish the optimum protocol for the isolation and culture of porcine muscle satellite cells. Mononuclear muscle satellite cells are a kind of adult stem cell, which is located between the basal lamina and sarcolemma of muscle fibers and is the primary source of myogenic precursor cells in postnatal muscle. Muscle satellite cells are a useful model to investigate the mechanisms of muscle growth and development. Although the isolation and culture protocols of muscle satellite cells in some species (e.g. mouse) have been established successfully, the culture system for porcine muscle satellite cells is very limited. In this study, we optimized the isolation procedure of porcine muscle satellite cells and elaborated the isolation and culture process in detail. Furthermore, we characterized the porcine muscle satellite cells using the immunofluorecence. Our study provides a reference for the isolation of porcine muscle satellite cells and will be useful for studying the molecular mechanisms in these cells.
PGC-1<i>α</i> Protects from Notch-Induced Kidney Fibrosis Development
Han, Seung Hyeok,Wu, Mei-yan,Nam, Bo Young,Park, Jung Tak,Yoo, Tae-Hyun,Kang, Shin-Wook,Park, Jihwan,Chinga, Frank,Li, Szu-Yuan,Susztak, Katalin American Society of Nephrology 2017 Journal of the American Society of Nephrology Vol.28 No.11