Characterization and mechanism analysis of penicillin G biodegradation with Klebsiella pneumoniae Z1 isolated from waste penicillin bacterial residue

Pu Wang,Huiling Liu,Huan Fu,Xiuwen Cheng,Bing Wang,Qinghua Cheng,Jian Zhang,Pan Zou 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.27 No.-

In this study, a strain Z-1 has been isolated from penicillin bacterial residue. The physicochemicalproperties and molecular phylogenetic analysis of the strain were characterized by atomic forcemicroscope (AFM) and scanning electron microscope (SEM) and 16SrDNA, indicating the strain belongsto genus Klebsiella pneumoniae. Furthermore, the biodegradation process of PENG was investigated with99.99% degradation efficiency. Afterwards, high performance liquid chromatography (HPLC) and LC-MS/MS analysis were performed to identify its degradation products. Moreover, the possible mechanism wasproposed and confirmed in detail. These results suggest Klebsiella pneumoniae Z1 become a promisingalternative for improving penicillin removal from environment

A comprehensive review of polysaccharide biopolymers for enhanced oil recovery (EOR) from flask to field

Wanfen Pu,Chao Shen,Bing Wei,Yang Yang,Yibo Li 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.61 No.-

To assist in understanding the progress of polysaccharide biopolymers in EOR, this review collects and summarizes the available data for the first time and thoroughly discusses the structure–property–EOR performance relationship from flask to field scale application. Attentions were given to xanthan, scleroglucan, hydroxyethylcellulose (HEC), carboxymethycellulose (CMC), welan gum, guar gum, schizophyllan, and other newly developed family members. This review provides a synopsis of EOR polysaccharides, which are rapidly emerging as eco-friendly and tough injectants compared to synthetic polymers.

A Gel-like Comb Micro-Block Hydrophobic Associating Polymer: Synthesis, Solution Property and the Sol-Gel Transition at Semi-Dilute Region

Wanfen Pu,Feng Jiang,Bing Wei,Yanli Tang,Yanyan He 한국고분자학회 2017 Macromolecular Research Vol.25 No.2

The gel-like comb micro block hydrophobic associating polymer (CBHAP) was synthesized by mixed micellar polymerization. The chemical structure of the copolymer was characterized by Fourier transform infrared spectroscopy (FT-IR). The rheological properties of CBHAP in fresh water and saline water at semi-dilute region were investigated. The experimental results show that, because of the intermolecular forces, shear thickening phenomenon can be observed obviously in brine solution and the Newtonian viscosity plateau could be observed clearly before 40 s-1. When the concentration reaches 3,000 mg/L, the G’ and G’’ becomes stable, indicating that CBHAP experiences the sol-gel transition after 3,000 mg/L. However, the curl structure is formed by adding salt, resulting in a higher concentration of the sol-gel transition than that in fresh water. Once the curl structure is destroyed by shearing or stretching, the intermolecular forces will take into effect and complete the sol-gel transition at semi-dilute region.

An adaptive plate at flapping wing’s trailing edge in promoting energy extraction performance

Longfeng Hou,Pu Yang,Dongmei Du,Bing Zhu 대한기계학회 2021 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.35 No.2

As is known, the maximum thickness position plays a key role in determining the energy extraction properties for flapping wing airfoils. Previous work demonstrated that the energy extraction efficiency might increase when we move the maximum thickness position towards the leading-edge direction. Inspired from this phenomenon, we propose in this work the addition of a flat plate at the tail of the flapping wings. Under two different Reynolds numbers (Re = 1100 and Re = 5×10 5 ), the effect of adding a rigid flat plate or a flexible flat plate to the trailing edge of the flapping wing on the flapping wing’s energy efficiency was investigated. The corresponding results were also compared with that of the original flapping wing. Results show that the addition of a rigid flat plate is sufficient for the amelioration of the flapping wing’s energy extraction efficiency in low Reynolds number cases. At high Reynolds number cases, the flapping wing with flexible flat plate trailing edge can maintain a relatively high energy extraction efficiency over a wide range of frequency, while the flapping wing with rigid flat plate trailing edge can only improve the energy extraction efficiency in the low frequency region. Therefore, we propose the application of flapping wings with flexible flat plate trailing edge for increasing the energy extraction efficiency under high Reynolds number conditions.

The differences between copper sulfate and tribasic copper chloride on growth performance, redox status, deposition in tissues of pigs, and excretion in feces

Zheng, Ping,Pu, Bei,Yu, Bing,He, Jun,Yu, Jie,Mao, Xiangbing,Luo, Yuheng,Luo, Junqiu,Huang, Zhiqing,Luo, Chenggui,Wang, Shaohui,Chen, Daiwen Asian Australasian Association of Animal Productio 2018 Animal Bioscience Vol.31 No.6

Objective: The objective of this experiment was to compare the effects of adding 130 mg/kg Cu from either copper sulfate (CS) or tribasic copper chloride (TBCC) on growth performance, mineral deposition in tissues, and the excretion in feces of pigs as well as changes in the mineral contents in tissues and feces when the supplemental Cu level was decreased from 130 mg/kg to 10 mg/kg. Methods: A total of 72 pigs ($32.6{\pm}1.2kg$) were randomly assigned to a CS diet or a TBCC diet with 6 pens per treatment. The trial lasted 102 d and included 3 phases (phase 1, 1 to 30 d; phase 2, 31 to 81 d; and phase 3, 82 to 102 d). The supplemental levels of Cu in the 2 treatments were 130 mg/kg in phase 1 and 2 and 10 mg/kg in phase 3. Results: The results showed that pigs fed the CS diet tended to have higher average daily gain than pigs fed the TBCC diet during d 1 to 81 (p<0.10). Compared with CS, TBCC increased the activities of aspartate transaminase (AST), ceruloplasmin, and superoxide dismutase in serum on d 30 (p<0.05). The TBCC decreased the Cu level in the liver on d 81 (p<0.05) and increased the Mn level in the liver on d 102 (p<0.05). The concentration of Cu in feces sharply decreased when the supplemental Cu level in diet changed from 130 mg/kg to 10 mg/kg in both diets (p<0.05). Conclusion: The result suggested that TBCC and CS had no significant difference on growth performance but TBCC had higher activities of AST and antioxidant enzymes and lower liver Cu than CS when pigs fed diets with 130 mg Cu /kg diet.

Association Between the Ku70-1310C/G Promoter Polymorphism and Cancer Risk: a Meta-analysis

Xu, Lu,Ju, Xiao-Bing,Li, Pu,Wang, Jue,Shi, Zhu-Mei,Zheng, Ming-Jie,Xue, Dan-Dan,Xu, Yan-Jie,Yin, Yong-Mei,Wang, Shui,You, Yong-Ping Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.2

Ku70 plays an important role in DNA double-strand break repair. Studies revealing conflicting results on the role of the Ku70-1310C/G promoter polymorphism on cancer risk led us to perform a meta-analysis to investigate this relationship. Ten case-control studies with 2566 cases and 3058 controls were identified. Odds ratios (ORs) and 95% confidence intervals (CIs) were used to assess the strength of associations. The overall results suggested no association between the Ku70-1310C/G promoter polymorphism and total cancer risk. However, on stratified analysis, significantly increased risks were observed among the Asian population (GG vs. CC: OR=1.50, 95%CI=1.10-2.06; GG vs. CC/CG: OR=1.47, 95%CI=1.07-2.01) and population-based case-control studies (GG vs. CC: OR=1.57, 95%CI=1.12-2.22; CG vs. CC: OR=1.35, 95%CI=1.11-1.64; CG/GG vs. CC: OR=1.37, 95%CI=1.14-1.65). Additionally, variant genotypes were associated with a significantly increased breast cancer risk (GG vs. CC: OR=1.80, 95%CI=1.26-2.56; GG vs. CC/CG: OR=1.40, 95%CI=1.01-1.95).

Dose-Dense Rituximab-CHOP versus Standard Rituximab-CHOP in Newly Diagnosed Chinese Patients with Diffuse Large B-Cell Lymphoma: A Randomized, Multicenter, Open-Label Phase 3 Trial

Xueying Li,He Huang,Bing Xu,Hongqiang Guo,Yingcheng Lin,Sheng Ye,Jiqun Yi,Wenyu Li,Xiangyuan Wu,Wei Wang,Hongyu Zhang,Derong Xie,Jiewen Peng,Yabing Cao,Xingxiang Pu,Chengcheng Guo,Huangming Hong,Zhao 대한암학회 2019 Cancer Research and Treatment Vol.51 No.3

Purpose Rituximab with cyclophosphamide, doxorubicin, vincristine, and prednisone administered every 3 weeks (R-CHOP-21) is the standard care for diffuse large B-cell lymphoma (DLBCL). It is unknown whether the dose-dense R-CHOP (R-CHOP-14) could improve the outcome of the disease in Asian population. Materials and Methods Newly diagnosed DLBCL patients were centrally, randomly assigned (1:1) to receive R-CHOP- 14 or R-CHOP-21. R-CHOP-14 was administered every 2 weeks, and R-CHOP-21 was administered every 3 weeks. Primary end point was disease-free survival (DFS). Secondary end points included overall survival (OS), progression-free survival (PFS), response rate and toxicities. Results Seven hundred and two patients were randomly assigned to receive R-CHOP-14 (n=349) or R-CHOP-21 (n=353). With a median follow-up of 45.6 months, the two groups did not differ significantly in 3-year DFS (79.6% for R-CHOP-14 vs. 83.2% for R-CHOP-21, p=0.311), 3-year OS (77.5% for R-CHOP-14 vs. 77.6% for R-CHOP-21, p=0.903), or 3-year PFS (63.2% for R-CHOP-14 vs. 66.1% for R-CHOP-21, p=0.447). Patients with an International Prognostic Index (IPI) score ! 2 had a poorer prognosis compared to those with an IPI score < 2. Grade 3/4 hematologic and non-hematologic toxicities were manageable and similar between R-CHOP-14 and R-CHOP-21. Conclusion R-CHOP-14 did not improve the outcome of DLBCL compared to R-CHOP-21 in Asian population. With manageable and similar toxicities, both of the two regimens were suitable for Asian DLBCL patients. For high-risk patients with IPI ! 2, new combination regimens based on R-CHOP deserve further investigation to improve efficacy.

Low temperature oxidation characteristics analysis of ultra-heavy oil by thermal methods

Yi-Bo Li,Ya-Fei Chen,Wan-Fen Pu,Hong Dong,Hao Gao,Fa-yang Jin,Bing Wei 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.48 No.-

To study the oxidation behavior of heavy oils, low temperature oxidation experiments under differentialtemperature and pressure conditions were carried out for both heavy oils (ordinary heavy and ultraheavyoils) with thermogravimetry (TG–DTG)/differential scanning calorimeter (DSC) analyses. And theresults indicated that the differences of physical properties and oxidation reactions for the heavy oilsascribe to the SARA compositions, H/C ratio and specific oxidation path. Owing to the asphaltenescontent, the ultra-heavy oil after the oxidation has more obvious coke deposition with lower activationenergy demand to make it possible for the in-situ combustion without combustion adjuvants.

Profile of disposition, tissue distribution and excretion of the novel anti-human immunodeficiency virus (HIV) agent W-1 in rats

Ying-Yuan Lu,Xiao-Wei Wang,Xin Wang,Wen-Bing Dai,Qiang Zhang,Pu Li,Ya-Qing Lou,Chuang Lu,Jun-Yi Liu,Guo-Liang Zhang 대한약학회 2016 Archives of Pharmacal Research Vol.39 No.7

The purpose of this study was to characterize the disposition, distribution, excretion and plasma protein binding of 6-benzyl-1-benzyloxymethyl-5-iodouracil (W- 1) in rats. Concentrations of W-1 within biological samples were determined using a validated high performance liquid chromatography method. The plasma protein binding of W-1 was examined by equilibrium dialysis method. After oral administration of W-1 (50, 100 and 200 mg/kg, respectively) in self-microemulsifying drug delivery system formulation, the pharmacokinetic parameters of W-1 were as follows: the peak plasma concentrations (Cmax) were 0.42, 1.50 and 2.55 μg/mL, the area under the curve (AUC0-t) were 0.89, 2.27 and 3.96 lg/h mL and the plasma half-life (t1/2) were 5.15, 3.77 and 3.77 h, respectively. Moreover, the prototype of W-1 was rapidly and extensively distributed into fifteen tissues, especially higher concentrations were detected in intestine, stomach and liver, respectively. The plasma protein binding of W-1 in rat, beagle dog and human were in the range of 97.96–99.13 %. This study suggested that W-1 has an appropriate pharmacokinetics in rats, such as rapid absorption, moderate clearance, and rapid distribution to multiple tissues. Those properties provide important information for further development W-1 as an anti-HIV-1 drug candidate.

Changes in Dust Activity in Spring over East Asia under a Global Warming Scenario

Qi Zong,Rui Mao,Dao-Yi Gong,Chenglai Wu,Bing Pu,Xingya Feng,Yijie Sun 한국기상학회 2021 Asia-Pacific Journal of Atmospheric Sciences Vol.57 No.4

Dust activity not only influences human health through dust storms but also affects climate at local and regional scales through the direct effects of dust aerosols on both solar and longwave radiative heating. In this study, based on dust simulations from seven Coupled Model Intercomparison Project Phase 5 (CMIP5) models, the spatial and temporal changes in dust activity over East Asia under a Representative Concentration Pathway 8.5 global warming scenario were examined for the periods of 2016– 2035 (P1), 2046–2065 (P2) and 2080–2099 (P3). The results show that the multimodel ensemble mean (MME) of the CMIP5 models largely captures the spatial distribution of dust emissions and dust optical depth (DOD) over East Asia during 1986–2005 (P0). The MME reproduces the increasing trend in dust emissions and DOD over dust sources in East Asia during P0. Accompanying emission reductions during P1 to P3, the DOD simultaneously decreases, and the evident DOD decline can also be found over downwind areas in eastern China and the Korean Peninsula. Simulations project increases in precipitation and the LAI (leaf area index). Simultaneously, the weakened East Asian trough leads to anomalous southerly winds and lower wind speeds at the surface. All these results indicate unfavorable conditions for dust emissions over the sources regions, resulting in a decreased DOD over East Asia during P1 to P3.