http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Bernardo R. Raymundo,오인록,김미정,김찬화 대한화학회 2019 Bulletin of the Korean Chemical Society Vol.40 No.12
The role of transgelin (TAGLN) in cancer has been discussed; however, the mechanisms underlying its regulation and correlation with MDA-MB-231 cell plasticity and migratory patterns remain unclear. We generated stable TAGLN-knockdown MDA-MB-231 cells and treated them with phorbol 12-myristate 13-acetate or transforming growth factor (TGF)-?. Chemotaxis, morphology, and invasion were assayed using three-dimensional matrices to evaluate cytoskeletal remodeling and migratory changes. Wound healing assays were conducted using cell inserts. TAGLN knockdown cells exhibited altered morphology due to cytoskeletal remodeling, yet only untreated and TGF?1-treated cells exhibited enhanced migration. Untreated and TGF?1-treated TAGLN knockdown cells showed increased N-WASP, ROCK1, and ROCK2 protein levels, which induce cytoskeletal remodeling. Evaluating phospholipase C?1 (PLC?1)-cofilin signaling-related proteins revealed that only TGF?1-treated TAGLN knockdown cells were influenced by PLC?1-cofilin signaling. Taken together, TAGLN knockdown is necessary for the TGF?1-mediated activation of PLC?1-cofilin pathway-driven amoeboid morphology and enhanced migratory properties in MDA-MB-231 cells.
Transgelin (TAGLN) Regulates IQGAP1 and Alters the Functions of Breast Cancer Cells
Bernardo R. Raymundo,오인록,Ling Xiu,김찬화 대한화학회 2020 Bulletin of the Korean Chemical Society Vol.41 No.10
Several studies have been conducted on the transgelin (TAGLN) protein and its critical role in cancer biology. However, the regulation of this protein and the way in which this regulation is correlated with the functions of IQ motif-containing GTPase-activating protein 1 (IQGAP1) in MDA-MB231 cells, remain unclear. We generated stable TAGLN-knockdown and TAGLN-overexpressing cells. These cells, along with their control counterparts, were cultured in the presence or absence of 17-AAG. The different cell groups were then subjected to functional assays to assess proliferation, chemotaxis, and invasion. TAGLN regulation was found to affect the efficacy of 17-AAG. The ability of TAGLN to influence the levels of IQGAP1 and its binding partners altered the critical functions of breast cancer cells. Therefore, the altered functionality of MDA-MB-231 cells, as a consequence of TAGLN regulation, is correlated with IQGAP1 signaling.
오인록,Bernardo Raymundo,Sung A Jung,Hyun Jung Kim,Jung-Keug Park,김찬화 대한화학회 2020 Bulletin of the Korean Chemical Society Vol.41 No.8
Extremely low-frequency electromagnetic fields (ELF-EMFs) (1?300?Hz) have been found to have practical applications in biological research. A case in point is the effect of ELF-EMF on the regulation of cell fate. In this study, we investigated the correlation between ELF-EMF stimulation of PPAR? to the stemness, tumorigenicity, and invasiveness of breast cancer stem cells in vitro. The CD44+/CD24? subpopulation of the breast CSCs was isolated from the MDA-MB-231 breast cancer cell line. The CSCs were then exposed to ELF-EMF and further assays were carried out. ELF-EMF increased the expression of PPAR? and other critical proteins leading to cell cycle arrest and reduced stemness as evidenced by decreased expression of stemness genes and reduced proliferation rate in ELF-EMF-exposed CSCs compared to that in nonexposed CSCs. There was a decrease in the tumor-forming and invasion ability of CSCs that were exposed to ELF-EMFs.
Aikins, Anastasia R,Kim, MiJung,Raymundo, Bernardo,Kim, Chan-Wha SAGE Publications 2017 Experimental biology and medicine Vol.242 No.6
<P>Vasculogenic mimicry (VM) is a non-classical mechanism recently described in many tumors, whereby cancer cells, rather than endothelial cells, form blood vessels. Transgelin is an actin-binding protein that has been implicated in multiple stages of cancer development. In this study, we investigated the role of transgelin in VM and assessed its effect on the expression of endothelial and angiogenesis-related genes during VM in MDA-MB-231 breast cancer cells. We confirmed the ability of MDA-MB-231 cells to undergo VM through a tube formation assay. Flow cytometry analysis revealed an increase in the expression of the endothelial-related markers VE-cadherin and CD34 in cells that underwent VM, compared with those growing in a monolayer, which was confirmed by immunocytochemistry. We employed siRNA to silence transgelin, and knockdown efficiency was determined by western blot analyses. Downregulation of transgelin suppressed cell proliferation and tube formation, but increased IL-8 levels in Matrigel cultures. RT-PCR analyses revealed that the expression of IL-8, VE-cadherin, and CD34 was unaffected by transgelin knockdown, indicating that increased IL-8 expression was not due to enhanced transcriptional activity. More importantly, the inhibition of IL-8/CXCR2 signaling also resulted in suppression of VM with increased IL-8 levels, confirming that increased IL-8 levels after transgelin knockdown was due to inhibition of IL-8 uptake. Our findings indicate that transgelin regulates VM by enhancing IL uptake. These observations are relevant to the future development of efficient antivascular agents.</P>