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한인호,Barbora Vagaska,이대형,박봉주,신성우,김정구,이승진,박종철 한국고분자학회 2011 Macromolecular Research Vol.19 No.7
Recently, three-dimensional polymer scaffolds were developed for tissue regeneration. Conventional prewetting processes require a lengthy processing time and have residue problems. In this study, a helium atmospheric pressure glow discharge (He-APGD) was employed as a pre-wetting process for porous poly-(L-lactide-co-ε-caprolactone)(PLCL) scaffold. The three minutes treated PLCL scaffold absorbed the culture media and contained grafted hydroxyl, carbonyl, and carboxyl groups. Moreover, it did not show cytotoxicity, did not require residual rinsing steps and showed a homogeneous cell distribution. The He-APGD treatment has a short processing time and promotes a homogenous cell distribution. Therefore, this pre-wetting process is expected to find many applications in the future.
Methods for Evaluation of the Chemotactic Migration of the Cells in 3D Biomimetic Matrix
유경은,Dohyun Kim,권병주,Barbora Vagaska,강재경,서혁진,이미희,박종철 한국생체재료학회 2012 생체재료학회지 Vol.16 No.3
Cell migration plays a critical role in various biological processes such as embryogenesis, metastasis of tumor cells and wound healing. Therefore, the migratory behavior of the cells has been researched with various influencing factors including chemoattractants. Most researches have done with 2 dimensional (2D) structured stratums to investigate the cell migration, however, this study suggests three different methods for in vitro studies of cell migratory behavior which are performed in biomimetic 3D structural environment by using collagen matrix. In the firstly introduced method, wound model was built up with a silicon insert and the chemoattractant, PDGF-bb was applied. Then, the movement of the cells to the wound area was observed. It was designed for examination of the potential of wound healing effect of certain agents. Other two methods are to study chemotactic migration of the cells in the 3D matrix in the presence of the chemoattractant. The orientation of the cell migration was examined by the under-agarose cell migration assay as the cells were seeded between the control collagen gel and PDGF-bb containing collagen gel. The cells located in the middle of the migrating chamber were affected by the components in both sides and the direction of their movement was determined. The last method was also performed in a 3D collagen matrix and the cell suspension was directly contacted to the chemoattractant. Therefore, the movement behavior of the cells was influenced by the agent. These protocols have different characteristics to evaluate the migration of the cells, and thus an appropriate method would be chosen for various studies of migration.
한인호,박종철,권병주,Barbora Vagaska,김봉진,강재경,이미희,김학희,Kang-Kyun Wang,김용록,안준성,이지민,현채영,정재환,임수진 한국고분자학회 2011 Macromolecular Research Vol.19 No.11
Plasma surface modifications of polymer scaffolds using biomolecules such as oxygen, nitrogen, and other active grafting molecules have been studied to enhance biological responses such as cell attachment, spreading,and proliferation. According to the reports, nitrogen grafting requires corrosive or mixture gas environment, or post treatment. This study aimed to evaluate a simple atmospheric pressure plasma surface modification in order to graft nitrogen derivatives and to promote biological responses. In this study, a polycarprolactone (PCL) film was modified within 10 min by argon atmospheric pressure discharge (Ar-APGD). Excited argon atoms, nitrogen atoms,oxygen atoms, and hydroxyl functional groups were observed from the optical emission spectra of the discharge. Decreased carbonyl functional groups and ether functional groups were observed; notably, immobilized nitrogen was observed on the PCL surface after the Ar-APGD treatment. Promoted neonatal Human Dermal Fibroblast (nHDF) growth patterns were observed on the Ar-APGD-treated surface.