트레드밀 운동이 일부 흡연 남자대학생의 혈액 성분과 항산화효소에 미치는 영향

방현수 김천대학교 2015 김천대학교 논문집 Vol.36 No.-

Purpose : The purpose of this study was to analyze the changes blood components and antioxidant enzymes of by treadmill exercise in smoking male college students. Method : The subjects were consisted of normal eight smoking male college students. They were from 20 to 25 years old. Treadmill exercise was conducted for 3 weeks and was comprised of 60 minutes for three times a week. All subjects measured blood components variables(Red Blood Cell; RBC, White Blood Cell; WBC, hematocrit, neutrophil), antioxidant enzymes variables(glutathione peroxidase, superoxide dismutase) before and after 3-weeks. Statistics analysis used to paired-t test. Result : The results of this study were summarized as follows: 1. The WBC was increased in the pre-post test(p<.05), but the RBC, hematocrit, neutrophil were not increased in the pre-post test(p>.05). 2. The glutathione peroxidase, superoxide dismutase were increased in the pre-post test(p<.05) Conclusion : In the present study, results indicate that treadmill exercise helps to improve the blood components(WBC) and antioxidant enzymes in smoking male college students.

健康한 韓國人의 動脈血液 가스 및 酸一鹽基 平衡値에 對한 觀察

崔震守,曺方煥,朴喜淑,金顯承,金瑛,白正敏,徐錫助 순천향의과대학 1979 Journal of Soonchunhyang Medical Science Vol.2 No.3

The purpose of this paper is to make an attempt in this investigation to determine normal adult values of arterial blood gas and acid-base balance in 53 healthy Korean male and female subjects of varying age and then compare with the data obtained by others. Determinations of the acid-base status of the arterial blood were performed at 37℃ by the Astrup method using a radiometer instruments, consisted of BGA2A/3-MK 2 system. The mean and standard errors of each component were as follows: pH 7.42±0.043, oxygen saturation 96.96±0.089%, pO?? 97.66±0.91mmHg, pCO?? 37.08±0.68mmHg, CO?? content 24.29±0.43mM/L, bicarbonate 23.26±0.68mM/L, buffer base 46.56±0.66mEg/L, and base excess -0.024±0.001mEg/S. Compared with normal adult values in literatures, all the components of the data appear to be in the same range without any significant difference.

전립선암의 예측인자로서 전환대 전립선특이항원의 유용성

김현학,권동득,오봉렬,박광성,류수방,박양일 全南大醫大 2001 전남의대학술지 Vol.37 No.3

비혈연간 동종골수이식에 의해 치료된 중증 재생불량성빈혈 1예

김현중,이홍기,이방훈,이창근,이종태,정현식,김원석,윤성수,강원기,박근칠,김대식,고영혜,박찬형 대한조혈모세포이식학회 1997 대한조혈모세포이식학회지 Vol.2 No.1

비혈연간 동종골수이식은 혈연간 동종골수이식이 불가능한 환자에서 기존의 면역역제요법으로 효과가 없었던 경우나 재발한 경우에 적응증이 될수 있다. 이식 거부와 이식편대숙주병의 발생이 골수 이식의 성공률을 감소시키므로, 보다 강력한 conditioning regimen의 사용과 T cell이 제거된 골수를 사용하는 것이 필요하다. 기존의 high dose corticosteroid나 cyclosporine에 대하여 불응성인 이식편대숙주병의 치료를 위해서 ATG와 Mycophenolate mofetil (Cellcept®) 의 사용이나 Tacrolimus, Methotrexate와 같은 다른 면역억제제치료를 고려해 볼 수 있다. It is known that allogenic bone marrow transplantation is the most effective treatment for aplastic anemia. However, this treatment is only applicable to a minority of patients because the proportion of patients who have HLA-matched siblings about 25% to 30% of all the aplastic anemia patients. In the case of the absence of HLA-matched donor, unrelated allogenic bone marrow transplantation is considered. However, (because of severe acute and chronic GVHD and graft failure) the success rate of this approach is only 20~30%. Recently, it was reported that increased immunosuppressive condition combined with T cell depletion of the marrow graft would result in greater success. There was no case of successfully treated aplastic anemia patient by unrelated allogenic bone marrow transplantation in Korea. Here, we report a case of severe aplastic anemia successfully treated with unrelated allogenic bone marrow transplantation.

심부목굽힘근 운동이 VDT 증후군이 유발된 대학생의 등세모근 근피로도에 미치는 영향

이규리 ; 방현수 김천대학교 2010 김천대학교 논문집 Vol.31 No.-

The purpose of this study were to compare and analyze two types of deep cervical flexion exercise, craniocervical flexion and cervical flexion, and to ascertain their muscle fatigue on Trapezius. The participants of the study were 24 college students who were appropriately cho-sen and randomly divided into two groups, the experimental group underwent a cranio-cervical flexion exercise program and the control group a cervical flexion exercise pro-gram. The two-week experiment program consisted of the exercises performed three times a week and the education directed once a week per each group. Upper Trapezius muscle and lower Trapezius muscle fatigue were measured prior to and two weeks after the commencement of the experiment and again after two weeks. At the completion of six wecks, both experimental programs showed the decrease of muscle fatigue (p<.05). But the control group, which the cervical flexion exercise was done on showed no significant changes of the upper-leſt Trapezius muscle fatigue (p>.05). And the craniocervical flexion exercise program which was done on the experimental group was more effective in reducing upper Trapezius muscle fatigue (p<.05). The results of this study showed deep cervical flexion excercise was effective in the treatment of Trapezius muscle fatigue, however craniocervical flexion exercise was more effective than cervical flexion exercise in reducing upper Trapezius muscle fatigue.

신경회로망의 대표적 모델들에 대한 분석 및 평가에 관한 연구 : Hopfield Machine, Boltzman Machine, Back-propagation Model

박찬호,김형호,방정희,이현수 慶熙大學校 1991 論文集 Vol.20 No.-

This paper discuss some problems and effectiveness in each neuron models which are currently wideley known as Hopfield Machine, Boltzman machine, and Back­propagation model through comparing and evaluating these models by simulation, simulation is performed for decimal digit recognition, and exclusive­OR problem for back­propagation.

Baculovirus를 利用한 Canine Parvovirus VP2蛋自質의 發現

朴鍾賢,宋載永,玄芳動,安動濬,姜永源,全茂炯,安壽煥 충남대학교 생물공학연구소 1998 생물공학연구지 Vol.6 No.-

국내에서 分離된 개파보바이러스주(V20주)의 VP2 遺傳子를 baculovirus system을 이용하여 발현시켜 다음의 結果를 얻었다. 1. 개파보바이러스의 VP2 유전자를 PCR에 의해 增幅하여 1755bp의 VP2遺傳子를 pUC19에 클로닝하여, 클로닝된 遺傳子를 polyhedrin promoter를 가지는 baculovirus expression vector인 pVL1393에 옮겨 VP2 발현벡터인 pVL1393-VP2를 얻을 수 있었다. 2. pVL1393-VP2 plasmid와 baculovirus DNA와의 homologous recombination에 의해 재조합바이러스인 VP2-BV를 얻을 수 있었으며, 그 발현효율은 2.000-5.000 HAU/0.05 ml이었다. 3. 免疫沈澱法에 의해 발현된 단백질은 개파보바이러스의 VP2단백질과 유사한 64 kb에 달하는 것이었으며, 血球凝集能을 지니고 있었다 4. 血球凝集能을 지닌 蛋白質이 여러 陽性血淸에 있어서 抗體수준을 測定할 수 있는지 개파보바이러스항원과의 相關性을 比較한 바 0.94 (n=125. p<0.01)의 相關係數를 보였다 5. 발현 VP2白은 virus-like particles를 形成하였으며, 그 크기로는 개파보바이러스와 비슷한 25 ㎚의 크기를 갖았다. Canine parvovirus(CPV) is a member of autonomous replicating parvoviruses and is aetiologically associated with enteritis and mycoarditis in puppies. The capsids of CPV are composed of three structural proteins: VP1, VP2 and VP3. The VP2 protein is the major component of capsid. The VP2 gene of a canine parvovirus. V20 strain isolated in Korea was cloned into baculovirus expression vector, and subsequently the VP2 protein was expressed by a recombinant baculovirus under the control of polyhedrin promoter. The recombinant VP2 protein expressed in Spodoptera frugiperda 9(Sf9) cells was detected by haemagglutination(HA) test and immunofluorescent antibody assay. Molecular weight of the recombinant VP2 protein expressed was estimated as 64Kd when tested by immunoprecipitation test using anti-CPV monoclonal antibody. In haemagglutination inhibition(Ⅲ) test. 8 HA units of the recombinant VP2 protein antigen was successfully utilized to determine a level of antibody against CPV in various positive sera. The recombinant VP2 protein showed also the capability to form virus like particles similar in size and appearance to the CPV virions.

Baculovirus를 이용한 Aujeszky's Disease Virus gⅢ 단백질 발현

송재영,이중복,현방훈,박종현,김병한,권창희,전무형,안수환 충남대학교 생물공학연구소 1993 생물공학연구지 Vol.3 No.-

The g Ⅲ gene located in U_L region of Yangsan strain, a field isolate of Aujeszky's disease virus (ADV) in Korea, was cloned into pTZ18R and sequenced. The gⅢ gene consisting of 1,437 nucleotides showed 98% sequence homology with that of Becker strain, a reference strain of ADV. The gene encoding gⅢ of Yangsan strain was placed under the control of Autographa californica nucleopolyhedrosis virus (AcNPV) polyhedrin promoter, and expressed by the derived recombinant baculovirus using Spodoptera frugiperda 9 (Sf9) cells. The expressed gⅢ was a protein with molecular weight of 72kd determined by immunoprecipitation and Western blotting assay using anti-ADV polyclonal antibodies and anti-gⅢ monoclonal antibody. The partially purified gⅢ protein was utilized as antigen in the radial immunodiffusion enzyme assay (RIDEA) to detect to specific antibody against ADV in pig sera. The results indicated that the sensitivity of RIDEA with the recombinant gⅢ protein antigen (98%) was as high as that with the conventional glycoprotein antigen extracted from the ADV infected cells. In addition, the false positive and false nagative reactions in gⅢ RIDEA were significantly reduced than the conventional glycoprotein RIDEA as judged from the results of standard serum neutralization test.

장기간 Risperidone 투여후 지연성으로 발생한 Neuroleptic Malignant Syndrome 1례

이헌정,조방현,김인,이민수 大韓神經精神醫學會 1999 신경정신의학 Vol.38 No.4

Risperidone은 비교적 최근에 개발된 항정신병약물이며, 그것이 가진 도파민 D2 수용체와 세로토닌2 수용체를 차단하는 작용기전, 추체외로 부작용과 지연성 운동장애가 적다는 점, 정신분열병의 음성증상에도 효과가 있다는 점에서 흔히 비전형적 약물로 불리운다. 그러나 이러한 비전형성에도 불구하고, 이미 전세계적으로는 15개 정도의 risperidone에 의한 신경이완제 악성증후군(neuroleptic malignant syndrome : NMS)의 보고가 있으며. 국내에서도 최근 하나의 증례가 보고되었다. 저자들은 정신분열병으로 장기간 risperidone으로 치료받던 젊은 남자 환자에서 치료 1년 9개월만에 발생된 NMS 1례를 경험하였기에 관련문헌의 고찰과 더불어 증례보고하고자 한다. Risperidone is a relatively new antipsychotic agent. It is often referred to as 'atypical', because it has a mechanism of action that blocks post synaptic dopamine-2 and serotonin-2 receptors, it is associated with fewer extrapyramidal side effects, it is not yet associated with tardive dyskinesia, and it may have some efficacy on negative symptoms of schizophrenia. In despite of its 'atypical' nature, there are already more than 15 reports of risperidone-induced neuroleptic malignant syndrome. Only one case of risperidone-induced NMS was reported recently in Korea. We report one case of delayed risperidone-induced neuroleptic malignant syndrome in young male patient and review the related articles.

미세혈관 협심증 환자의 임상상 및 지표에 관한 연구

박상호,온영근,김현정,이태훈,길효욱,김현건,박수진,송해정,한대희,방덕원,신원용,이내희,김성구,권영주 순천향의학연구소 2003 Journal of Soonchunhyang Medical Science Vol.9 No.1

Background : Microvascular angina or syndrom X is heterogenous and encompasses different pathogenetic entities. Several studies suggest that microvascular angina have good prognosis and occurs predominantly in postmenopausal women. Lipoprotein (a), total cholesterol, LDL-cholesterol, HDL-cholesterol, triglyceride, homocystein, CRP and fibrinogen are the independent risk factor of acute coronary syndrome and are the chief underlying cause of atherosclerosis. We investigated clinical characteristics and markers in patients with microvascular angina. Methods : We studies 23 patients diagnosed with microvascular angina and 16 control group with negative treadmill exercise test and no significant coronary angiogram. We investigated clinical characteristics in patients group and evaluated symptom during a follow-up period of 4.2± 2.5 month. Exercise time in treadmill exercise test was compared between patients and control group. We assessed the level of markers, such as lipoprotein (a), total cholesterol, LDL-cholesterol, HDL-cholesterol, triglyceride, homocystein, CRP and ESR in two groups. Results : There is no difference in age between two groups. Patients group had a tendency to increase in female, hypertension, DM, and smoking history, but there was no statistical significance difference. Exercise duration time was significantly decreased in patients with microvascular angina (p<0,05). Serum lipoprotein (a), total cholesterol, LDL-cholesterol, HDL-cholestrol, Triglyceride, ESR, Fibrinogen and Homocystein value were no significant difference between microvascular angina and control group. Only CRP was significantly high in patients with microvascular angina (p<0.05). Conclusion : There was no statistical significance difference in age, sex, hypertension, DM and smoking history between two groups but patients group had a tendency to increase the prevalence in hypertension, DM and smoking history, female. Also, Symptom improvement and disappearance in follow-up was presented in 11 patients of 19(57.9%). Exercise tolerance was worse in patients group than control group. CRP value was significantly higher in patients group than control group. There was no statistical significance difference in lipoprotein (a), total cholesterol, LDL-cholesterol, HDL-cholesterol, triglyceride, homocystein, ESR and Fibrinogen between two groups.