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      • Aerosol radiative forcing over a high-altitude station Merak, in the trans-Himalayan region during advection of anthropogenic events from the Indo-Gangetic Plain

        Ningombam, S.S.,Bagare, S.P.,Srivastava, A.K.,Sohn, B.J.,Song, H.J.,Larson, E. Pergamon Press ; Elsevier [distribution] 2014 Atmospheric environment Vol.98 No.-

        Advection of anthropogenic aerosols from the Indo-Gangetic Plain (IGP) and dust aerosols from distant deserts towards a high-altitude station Merak, in the trans-Himalayan region are reported during June-July 2011. In order to differentiate the advection event, aerosol optical properties were examined during aged background conditions at the site. During the aged background conditions, aerosol optical depth (AOD at 500 nm) and Angstrom exponent (α) at the station were ~0.06 and 1.36, respectively which were increased to 0.13 and 1.62, respectively during the advection event. Further, a strong signature of fine-mode aerosol volume size distribution, dominated by absorbing aerosols, was observed during the advection event. The average atmospheric forcing during the aged background condition was found to be 0.57 Wm<SUP>-2</SUP> (with corresponding heating rate of 0.05 Kday<SUP>-1</SUP>) and these results were enhanced to 2.58 Wm<SUP>-2</SUP> (with corresponding heating rate of 0.22 Kday<SUP>-1</SUP>) during the advection event. The present study reveals that during the advection event, heating rate in the atmosphere was increased by about four times than the aged background condition. Such atmospheric warming in the region may influence the melting of the Himalayan glaciers and consequently it may effect the local atmospheric circulation.

      • Poster Session : PS 0380 ; Infectious Disease ; Prevalence of Escherichia Coli O157:H7 Causing Children Diarrhea in Sirte, Libya

        ( Shaban Ramadan Abdelsalam Bagar ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

        Background: E. coli O157: H7 is a cause of acute infectious diarrhoea in humans and the leading cause of haemolytic uremic syndrome, especiallyamong children. E. coli O157:H7 was known as Verotoxin producing E. Coli (VTEC).It is also called as Shiga toxin producing E. coli(STEC). More than 50 serotypes of EHEC have been isolated. We undertook a study to Estimate the incidence of Escherichia coli O157:H7 infection in diarrheic children attending Ibn Sinna Hospital, Sirte - Libya Methods: 124 stool specimens of diarrheic children were collected at paediatric department in Ibn Sinna teaching hospital in the periodfrom September 2009 to may 2010. All the samples were cultured on SMAC.Non sorbitol - fermenting colonies are tested for the somatic O157 antigen before being confi rmed as E. Coli O157.The colonies are gram stained and confi rmed with O157 antiserum or latex reagent. These colonies were later biochemically confi rmed as E. coli using API20E and reported as presumptively positive for E. coli O157:H7. Results: 124 stool samples which were obtained from 124 diarrheic children contained 127 bacterial isolates belonging to 6 different genera or strains, as detected on SMAC plates Only 4 isolates were non-sorbitol fermenting colonies on SMAC agar. These sorbitol-negatives isolates were later identifi ed serologically and biochemically as: E. COLI O157:H7, one isolate; Shigella sonnei, one isolate; Enterobcater sp, two isolates; 123 isolates fermented sorbitol on SMAC agar, and later identifi ed serologically and biochemically as:Pseudomonas sp. Conclusions: Among 124 stool specimens, only 1(0.7%) was positive for E.coli O157:H7 Magnitude of the problem due to E. coli O157:H7 appears to be not much at Sirte but, because of its ability to cause complication, E. coli O157:H7 should be screened in routine culture and identifi ed. Out of 124 diarrhea samples tested, only four cases (3.2%).

      • Poster Session : PS 0442 ; Infectious Disease ; Distribution of Hepatitis B Virus Genotypes in Libya Using PCR-Based Diagnostics

        ( Ramadan Abdelsalam Shaban Bagar ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

        Background: Hepatitis B virus (HBV) represents a global health challenge due to its worldwide distribution and serious complications. The HBV genotypes have different infi uence on clinical picture, response to treatment and the long-term prognosis. Eight genotypes have been identifi ed and a ninth is on the horizon, with distinct geographical distributions. Aim: The aim of this study was to investigate the prevalence of HBV genotypes in Libyan patients. Methods: Quantitative real-time PCR assay was carried out on 121 clinical specimens.The specimens were extracted and amplifi ed using INNO-LiPA HBV Genotyping Primers and run on a 2% agarose gel, then hybridized by INNO-LiPA HBV Genotyping assay that allows the identifi cation of HBV genotypes A to H. Results: DNA Quantity ranged from < 50 to 3.6x10 IU/ml, 85 specimens with DNA quantity more than 1.000 copies/ml were extracted and amplified, 60 specimens which had bands on 2% agarose gel were hybridized by the INNO-LiPA HBV genotyping assay. Three genotypes were detected; D in 54 specimens (90%), A and E in 1 specimen for each (1.70%) and 4 specimens (6.70%) were mixed genotypes D/E. Conclusions: This study indicates that the predominant genotype in Libyan patients is genotype D.

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