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항생제 다제내성균 Staphylococcus aureus SA2로부터 분리한 테트라사이클린 내성 플라스미드 pKH6의 염기서열
이대운,윤성준,김우구,신철교,임성환,이백락,문경호 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.4
한국에서 임산균주로부터 분리한 테트라사이클린(Tc) 내성 플라스미드 pKH6의 전체 염기서열을 결정하여 가장 대표적인 Tc 내성 플라스미드인 pT181과 비교하였다. pKH6의 전체 길이는 4439bp로 pT181과 동일하게 나타났으며 염기서열은 7개의 염기에서 차이를 보였다. 전부 다 염기쌍이 치환된 형태로 나타났으며 그 중 3개는 coding 부위에서 일어났다. coding 부위에서 일어난 경우에도 1개는 동일한 아미노산을 coding 하였기 때문에 pT181과 차이가 없었으며 RepC와 Pre 단백질에 있어 각각 1개의 아미노산에서 차이를 나타내었다. The complete nucleotide sequence of pKH6, a tetracycline-resistance (Tc^r) plasmid isolated from multi-drug resistant Staphlococcus aureus SA2, has been determined and compared with that of the staphylococcal Tc^r plasmid pT181. The nucleotide sequences of the two plasmids are in agreement except for 7 nucleotides. All differences are caused by base pair substitutions. Among 6 substitutions, 3 occurred in coding regions. However, only tow base substitutions in coding regions resulted in changes of amino acid sequences in two different ORFs of repC and Pre proteins.
Lee, Seoung Rak,Roh, Hyun-Soo,Lee, Seul,Park, Hyun Bong,Jang, Tae Su,Ko, Yoon-Joo,Baek, Kwan-Hyuck,Kim, Ki Hyun Elsevier 2018 Journal of Functional Foods Vol.40 No.-
<P><B>Abstract</B></P> <P> <I>Morchella esculenta</I> (Morchellaceae), one of the most widely appreciated wild edible mushrooms, has demonstrated antitumor activity both <I>in vivo</I> and <I>in vitro</I>. However, the molecular basis underlying its antitumor activity has not yet been elucidated. In this study, we examined the biological effects of the MeOH extract of <I>M. esculenta</I> fruiting bodies on four human lung adenocarcinoma cell lines. Bioactivity-guided fractionation of the MeOH extract followed by chemical investigation of its most cytotoxic hexane-soluble fraction led to the isolation of eight compounds (<B>1</B> −<B>8</B>), including three fatty acids and five sterols. This is the first report of the isolation of compounds <B>1</B> and <B>3</B>–<B>7</B> from <I>M. esculenta</I>. Among the eight isolated compounds tested, compounds <B>1</B>, <B>3,</B> and <B>5</B> exhibited the most potent cytotoxicity to human lung cancer cell lines, with IC<SUB>50</SUB> values ranging from 156.9 to 278 M; this activity was mediated by induction of apoptosis.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Morel mushroom (<I>Morchella esculenta</I>) is a wild edible mushroom. </LI> <LI> Bioactivity-guided isolation of the extract led to the isolation of eight compounds. </LI> <LI> Compounds <B>1</B> and <B>3</B>–<B>7</B> were isolated for the first time from <I>M. esculenta</I>. </LI> <LI> Compounds <B>1</B>, <B>3</B>, and <B>5</B> exhibited potent cytotoxicity to human lung cancer cells. </LI> <LI> The activity of compounds <B>1</B>, <B>3</B>, and <B>5</B> was mediated by the induction of apoptosis. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
An Improved and Efficient Method for Cosmid Cloning
LEE, BAEK-RAK,WEISBLUM, AERNARD 한국미생물 · 생명공학회 1994 Journal of microbiology and biotechnology Vol.4 No.3
A general improved procedure for preparation of a cosmid library based on the use of a pBLcosT vector was described. The vector was modified to contain 2 tandem Xcml sites and was digested with Xcml to yield 2 terminal 3'T overhangs, capable of ligation with the insert that contains 2 terminal complementary 3'A overhangs. The resultant ligation mixture was packaged and a cosmid library in Escherichia coli was established.
벼(Oryza sativa L.)의 지상부로부터 adenosine과 phlomuroside의 분리 및 동정
정락훈 ( Rak Hun Jeong ),이대영 ( Dae Young Lee ),조진경 ( Jin Gyeong Cho ),백윤수 ( Yoon Su Baek ),서경화 ( Kyeong Hwa Seo ),이동걸 ( Dong Geol Lee ),강희철 ( Hee Cheol Kang ),김지영 ( Ji Young Kim ),백남인 ( Nam In Baek ) 한국응용생명화학회(구 한국농화학회) 2014 Journal of Applied Biological Chemistry (J. Appl. Vol.57 No.4
벼 지상부를 MeOH 수용액으로 추출하고 이 추출물을 n-hexane, EtOAc, n-BuOH, 및 H2O 분획으로 나누었다. n-BuOH 분획에 대하여 silica gel, ODS 및 Sephadex LH-20 column chromatography 를 반복실시 하여 2종의 화합물을 분리하였다. NMR, IR 및 MS data를 해석하여 각각 adenosine (1)과 phlomuroside (2)로 구조동정 하였다. 이들 화합물들은 벼에서는 처음으로 분리되었다. Fresh and chopped aerial parts of Oryza sativa were extracted in 80% aqueous mehthanol, and the concentrated extract was successively partitioned in n-hexane, ethyl acetate (EtOAc), n-butanol (n-BuOH), and H2O fractions. From the n-BuOH fraction, two compounds were isolated through repeated silica gel and ODS column chromatography (c.c.). Based on nuclear magnetic resonance (NMR), mass spectrometry and infrared spectroscopy spectroscopic data, the compounds were identified to be adenosine (1) and phlomuroside (2). Especially, the configuration of both the anomer hydroxyl groups was determined as β from the coupling constants of the anomer protons (J =6.0 and 7.6 Hz) in the 1H-NMR spectra. This is the first report for the isolation of these compounds from Oryza sativa L.
New flavonolignan glucoside from the aerial parts of Oryza sativa
Jeong, Rak-Hun,Lee, Dae-Young,Cho, Jin-Gyeong,Seo, Kyeong-Hwa,Lee, Jae-Woong,Lee, Min-Ho,Seo, Woo-Duck,Kang, Hee-Cheol,Kim, Geum-Soog,Noh, Hyung-Jun,Lee, Youn-Hyung,Baek, and Nam-In Springer-Verlag 2014 Chemistry of natural compounds Vol.49 No.6
벼(Oryza sativa L.) 지상부로부터 신규 Flavonolignans 및 Lignan glycosides의 분리 및 동정
정락훈(Rak-Hun Jeong),이대영(Dae-Young Lee),조진경(Jin-GyeongCho),Sabina Shrestha,박지해(Ji-Hae Park),서경화(Kyeong-Hwa Seo),이민호(Min-Ho Lee),노영덕(Yeong-Deok Rho),백남인(Nam-In Baek),이대영(Dae-Yung Lee),서우덕(Woo-Duck Seo),강 한국약용작물학회 2012 한국약용작물학회 학술대회논문집 Vol.2012 No.2
S-476 : Paraneoplastic neutrophilia originated from anaplastic transformation of thyroid carcinoma
( Jeong Su Baek ),( Sung Hyun Lee ),( Ae Ri Cho ),( Joo Young Lee ),( Jeong Mi Jang ),( Hyo Rak Lee ),( Seung Suk Lee ) 대한내과학회 2013 대한내과학회 추계학술대회 Vol.2013 No.1
Anaplastic thyroid carcinoma can arise from preexisting differentiated carcinoma and generally show dire prognosis. It usually occurs in the intrathyroid or regional lymph node, but can be developed in the metastatic site rarely. We report a case of an 69-year old man who, 7 months after being treated with total thyroidectomy and I-131 therapy for papillary thyroid carcinoma, presented with anaplastic transformation in pleural metastatic site. Another interesting point in our case is patient showed an high white blood cell count. We performed him bone marrow biopsy for evaluating the cause of leukocytosis. But, there was no bone marrow involvement of malignant cells and no evidence of hematologic malignancy. Based on elevated serum granulocyte colony-stimulating factor (G-CSF) levels, it maybe due to paraneoplastic leukocytosis. And his WBC count decreased temporarily after systemic chemotherapy. Considering his clinical course, I-131 therapy maybe accelerate to anaplastic transformation. Clinicians, who treat well differentiated thyroid carcinoma, should be aware of the possibility of anaplastic transformation and should keep in mind that leukocytosis can be a initial sign of that change.
Jeong Su Baek,Sung Hyun Lee,Ae Ri Cho,Joo Young Lee,Jeong Mi Jang,Seung Suk Lee,Hyo Rak Lee 조선대학교 의학연구소 2014 The Medical Journal of Chosun University Vol.39 No.1
Marked neutrophilia associated with neoplasia is a relatively rare finding and has been considered as a paraneoplastic manifestation. Thyroid cancer seldom presents with paraneoplastic leukocytosis. We report on a case of a 69-year-old man who presented with paraneoplastic leukocytosis seven months after undergoing total thyroidectomy and I-131 therapy for treatment of papillary thyroidcarcinoma. We found neither bone marrow involvement of malignant cells nor hematologic malignancy. Based on elevated levels of serum granulocyte colony-stimulating factor, we concluded that the cause was paraneoplastic leukocytosis. Another interesting point was the anaplastic transformation in the pleural metastatic site. It usually occurs in the intrathyroid or regional lymph node.