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Bae, Jeehyeon,Won, Miae,Kim, Dae-Yeon,Kim, Jong-Hyeok,Kim, Yong-Man,Kim, Young-Tak,Nam, Joo-Hyun,Suh, Dae-Shik Blackwell Scientific Publications 2012 International journal of gynecological cancer Vol.22 No.4
<P>The main methods of treatment of endometrial carcinoma are hysterectomy and bilateral oophorectomy with lymphadenectomy. However, another option for hormonal treatment of endometrial carcinoma, the use of progesterone in young patients to preserve childbearing capacity, has been reported, and a high remission rate has been described in well-selected stage I, grade 1 endometrial cancer. Although it is intriguing that hormonal therapy alone can treat endometrial carcinoma without surgery or cytotoxic chemotherapy, the molecular basis for the effects of progesterone on endometrial carcinoma is not clearly known. MicroRNAs (miRNAs) are a class of naturally occurring small, noncoding RNA molecules that control cellular function and are known to function as both tumor suppressors and oncogenes. Thus, in the present study, changes in the miRNA profile of endometrial carcinoma cells on progesterone treatment were studied.</P>
KIM, Jae-Hong,BAE, Jeehyeon The Society for Reproduction and Development 2014 The Journal of reproduction and development Vol.60 No.1
<P><B>Abstract</B></P><P> FOXL2 is an essential transcription factor that is required for proper development of the ovary and eyelid. Mutations in FOXL2 cause an autosomal dominant genetic disorder, blepharophimosis-ptosis-epicanthus inversus syndrome (BPES). BPES type I patients have eyelid malformation and premature ovarian failure leading to infertility, whereas women with type II BPES are fertile or subfertile. In the present study, we evaluated and compared apoptotic and antiproliferative activities of wild-type (WT) and mutant FOXL2 proteins found in BPES type I and II in human granulosa cell tumor-derived KGN cells. Ectopic expression of WT FOXL2 induced apoptosis and inhibited cell cycle progression in human granulosa cells. In contrast, mutated FOXL2s found in BPES type I significantly reduced these activities, whereas mutated FOXL2s in BPES type II showed intermediate activities. Furthermore, mutant FOX L2 proteins were defective in activating transcription of target genes including <I>Caspase 8</I>, <I>TNF-R1</I>, <I>FAS</I>, <I>p21</I>, and <I>BMP4</I>, which regulate apoptosis, proliferation, and differentiation of granulosa cells. Thus, decreased apoptotic and antiproliferative activities caused by mutant forms of FOXL2 found in BPES patients may at least partially contribute to the pathophysiology of ovarian dysfunction.</P>
Development of DNA aptamers specific for small therapeutic peptides using a modified SELEX method
Lee Jaemin,Ryu Minkyung,Bae Dayeong,Kim Hong-Man,Eyun Seong-il,Bae Jeehyeon,Lee Kangseok 한국미생물학회 2022 The journal of microbiology Vol.60 No.7
Aptamers are short single-stranded DNA or RNA oligonucleotides capable of binding with high affinity and specificity to target molecules. Because of their durability and ease of synthesis, aptamers are used in a wide range of biomedical fields, including the diagnosis of diseases and targeted delivery of therapeutic agents. The aptamers were selected using a process called systematic evolution of ligands by exponential enrichment (SELEX), which has been improved for various research purposes since its development in 1990. In this protocol, we describe a modified SELEX method that rapidly produces high aptamer screening yields using two types of magnetic beads. Using this method, we isolated an aptamer that specifically binds to an antimicrobial peptide. We suggest that by conjugating a small therapeutic-specific aptamer to a gold nanoparticle-based delivery system, which enhances the stability and intracellular delivery of peptides, aptamers selected by our method can be used for the development of therapeutic agents utilizing small therapeutic peptides.
Kim, In Jai,Bae, Jeehyeon,Lim, Sang Wook,Cha, Dong Hoon,Cho, Hyo Jin,Kim, Sun,Yang, Dong Ho,Hwang, Seong Gyu,Oh, Doyeun,Kim, Nam Keun Pergamon Press 2007 Thrombosis research Vol.119 No.5
<P><B>Abstract</B></P><P><B>Introduction</B></P><P>Endothelium-derived nitric oxide (NO) is synthesized from <SMALL>L</SMALL>-arginine by endothelial nitric oxide synthase (eNOS) encoded by the eNOS3 gene on chromosome 7. The effects of the eNOS polymorphisms with the risk of coronary artery disease are conflicting. In this study, we investigated the association of the eNOS genotypes with coronary artery disease in Koreans.</P><P><B>Materials and methods</B></P><P>A case-control study was performed to evaluate the association between the eNOS −786T>C, 4a4b, or 894G>T polymorphism and coronary artery disease. 147 consecutive patients with coronary artery disease and 222 healthy controls were recruited. The genotypes of eNOS −786T>C and 894G>T polymorphisms were determined by the polymerase chain reaction-restriction fragment length polymorphism analysis. The genotypes of a 27?bp insertion/deletion in intron 4 (eNOS 4a4b) were determined by the banding pattern on gel electrophoresis.</P><P><B>Results</B></P><P>The eNOS −786T>C (odds ratio [OR]; 1.61, 95% confidence interval [CI]; 0.97–2.69), 894G>T (OR; 1.12, 95% CI; 0.65–1.92) and 4a4b (OR; 1.44, 95% CI; 0.87–2.39) polymorphisms were not an independent predisposition factor to coronary artery disease. However, a subgroup analysis adjusted with various cardiovascular risk factors confirmed positive association of the −786T>C polymorphism in CAD patients with hypertension and a smoking history and also a significant association of the intron 4 genotypes with a smoking history, but no significance has been found in the eNOS polymorphisms of 894G>T upon any risk adjustment. In this study we also found that the distribution of heterozygotes (−786TC, 894GT, and 4a4b) and variant homozygotes for the −786C, 894T, and intron 4a alleles of eNOS in Koreans were significantly lower than in Caucasian populations.</P><P><B>Conclusions</B></P><P>The present study demonstrates that polymorphisms of the eNOS −786T>C and 4a4b are associated with coronary artery disease with adjustments for cardiovascular risk factors in the Koreans.</P>
Selective Monitoring and Imaging of Eosinophil Peroxidase Activity with a J-Aggregating Probe
Kim, Tae-Il,Hwang, Byunghee,Lee, Boeun,Bae, Jeehyeon,Kim, Youngmi American Chemical Society 2018 JOURNAL OF THE AMERICAN CHEMICAL SOCIETY - Vol.140 No.37
<P>The specific detection of eosinophil peroxidase (EPO) activity requires the difficult distinction between hypobromous acid generated by EPO and hypochlorous acid generated by other haloperoxidases. Here we report a fluorogenic probe that is halogenated with high kinetic selectivity (≥1200:1) for HOBr over HOCl. Heavy-atom effects do not quench the dibrominated product because of its self-assembly into emissive J-aggregates that provide a turn-on signal. Applications of this fluorogen to EPO activity assays, dipstick sensors, fluorescence imaging of EPO activity, assays of oxidative stress in cancer cells, and immune response detection in live mice are reported.</P> [FIG OMISSION]</BR>
Excimer Emission-Based Fluorescent Probe Targeting Caspase-3
Kim, Tae-Il,Jin, Hanyong,Bae, Jeehyeon,Kim, Youngmi American Chemical Society 2017 ANALYTICAL CHEMISTRY - Vol.89 No.19
<P>A fluorescent probe based on an excimer-forming benzothiazolyl-cyanovinylene (<B>CV</B>) dye was developed to target the apoptotic protease caspase-3. Upon the action of caspase-3, the water-soluble fluorescent probe Ac-DEVD-<B>NH-CV</B>, which is weakly green emissive in aqueous solution, is converted to hydrophobic <B>CV-NH<SUB>2</SUB></B>, which spontaneously aggregates. Aggregation of <B>CV-NH<SUB>2</SUB></B> promotes excimer emission of the <B>CV</B> dye, which allows for the study of caspase-3 activity <I>in vitro</I> and for imaging the activity of the enzyme in living cells because of the large red shift and enhanced fluorescence signal of the probe.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancham/2017/ancham.2017.89.issue-19/acs.analchem.7b02790/production/images/medium/ac-2017-02790a_0006.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ac7b02790'>ACS Electronic Supporting Info</A></P>