Development of an antibody-based diagnostic method for the identification of Bemisia tabaci biotype B

Baek, J.H.,Lee, H.J.,Kim, Y.H.,Lim, K.J.,Lee, S.H.,Kim, B.J. Academic Press 2016 Pesticide biochemistry and physiology Vol.131 No.-

<P>The whitefly Bemisia tabaci is a very destructive pest. B. tabaci is composed of various morphologically undistinguishable biotypes, among which biotypes B and Q, in particular, draw attention because of their wide distribution in Korea and differential potentials for insecticide resistance development. To develop a biotypespecific protein marker that can readily distinguishes biotypes B from other biotypes in the field, we established an ELISA protocol based on carboxylesterase 2 (COE2), which is more abundantly expressed in biotypes B compared with Q. Recombinant COE2 was expressed, purified and used for antibody construction. Polydonal antibodies specific to B. tabaci COE2 [anti-COE2 pAb and deglycosylated anti-COE2 pAb (DG anti-COE2 pAb)] revealed a 3-9-fold higher reactivity to biotype B COE2 than biotype Q COE2 by Western blot and ELISA analyses. DG anti-COE2 pAb exhibited low non-specific activity, demonstrating its compatibility in diagnosing biotypes. Western blot and ELISA analyses determined that one of the 11 field populations examined was biotype B and the others were biotype Q suggesting the saturation of biotype Q in Korea. DG anti-COE2 pAb discriminates B. tabaci biotypes B and Q with high specificity and accuracy and could be useful for the development of a B. tabaci biotype diagnosis kit for on-site field applications. (C) 2016 Elsevier Inc. All rights reserved.</P>

Early Regulation of Viral Infection Reduces Inflammation and Rescues Mx-positive Mice from Lethal avian Influenza Infection

Song, M.S.,Cho, Y.H.,Park, S.J.,Pascua, P.N.Q.,Baek, Y.H.,Kwon, H.I.,Lee, O.J.,Kong, B.W.,Kim, H.,Shin, E.C.,Kim, C.J.,Choi, Y.K. American Association of Pathologists and Bacteriol 2013 The American journal of pathology Vol.182 No.4

Differing sensitivity of influenza A viruses to antiviral effects of the Myxovirus resistance (Mx) protein implies varying global gene expression profiles in the host. The role of Mx protein during lethal avian influenza (AI) virus infection was examined using Mx1-deficient C57BL/6 (B6-Mx1<SUP>-/-</SUP>) and congenic Mx1-expressing (B6-Mx1<SUP>+/+</SUP>) mice infected with a virulent, mouse-adapted avian H5N2 Ab/Korea/ma81/07 (Av/ma81) virus. After infection, B6-Mx1<SUP>+/+</SUP> mice were completely protected from lethal AI-induced mortality, and exhibited attenuated clinical disease and reduced viral titers and pathology in the lungs, compared with B6-Mx1<SUP>-/-</SUP> mice. Transcriptional profiling of lung tissues revealed that most of the genes up-regulated after infection are involved in activation of the immune response and host defense. Notably, more abundant and sustained expression of cytokine/chemokine genes was observed up to 3 dpi in B6-Mx1<SUP>-/-</SUP> mice, and this was associated with excessive induction of cytokines and chemokines. Consequently, massive infiltration of macrophages/monocytes and granulocytes into lung resulted in severe viral pneumonia and potentially contributed to decreased survival of B6-Mx1<SUP>-/-</SUP> mice. Taken together, our data show that dysregulated gene transcriptional activity corresponded to persistent induction of cytokine/chemokines and recruitment of cytokine-producing cells that promote inflammation in B6-Mx1<SUP>-/-</SUP> mouse lungs. Thus, we provide additional evidence of the interplay of genetic, molecular, and cellular correlates governed by the Mx1 protein that critically determine disease outcome during lethal AI virus infection.

Hardening of Bi-Te based alloys by dispersing B₄C nanoparticles

Jung, S.J.,Park, S.Y.,Kim, B.K.,Kwon, B.,Kim, S.K.,Park, H.H.,Kim, D.I.,Kim, J.Y.,Hyun, D.B.,Kim, J.S.,Baek, S.H. Elsevier Science 2015 Acta materialia Vol.97 No.-

Thermoelectric devices have attracted a great attention for renewable energy harvesters and solid-state coolers. For practical applications, the mechanical properties of thermoelectric materials become critical for the device reliability, a persistent performance with a long time and high operation cycles. Bi-Te based single-crystals, mostly used in commercial thermoelectric devices, are intrinsically brittle with weak van der Waals bonding, often leading to device failures such as crack and debonding during fabrication and operation. Thus, it is highly desirable to enhance the mechanical property of Bi-Te based alloys as well as the thermoelectric property. Here, we investigate the effect of B<SUB>4</SUB>C nanoparticles (less than 0.5wt%) dispersed in p-type Bi<SUB>0.4</SUB>Sb<SUB>1.6</SUB>Te<SUB>3</SUB> matrix on the mechanical properties. X-ray diffraction (XRD) result confirms that B<SUB>4</SUB>C-dispersed Bi<SUB>0.4</SUB>Sb<SUB>1.6</SUB>Te<SUB>3</SUB> has a single phase. We observe that the grain size of Bi<SUB>0.4</SUB>Sb<SUB>1.6</SUB>Te<SUB>3</SUB> becomes decreased with the B<SUB>4</SUB>C nanoparticle concentration by electron backscatter diffraction (EBSD) technique. Hardness, Young's modulus, and flexural strength of B<SUB>4</SUB>C-dispersed Bi<SUB>0.4</SUB>Sb<SUB>1.6</SUB>Te<SUB>3</SUB> are enhanced, compared to the B<SUB>4</SUB>C-free Bi<SUB>0.4</SUB>Sb<SUB>1.6</SUB>Te<SUB>3</SUB> polycrystals. On the other hand, the thermoelectric figure-of-merit of B<SUB>4</SUB>C-dispersed Bi<SUB>0.4</SUB>Sb<SUB>1.6</SUB>Te<SUB>3</SUB> is almost identical to that of the pure Bi<SUB>0.4</SUB>Sb<SUB>1.6</SUB>Te<SUB>3</SUB>. Such enhancements of the mechanical properties of the B<SUB>4</SUB>C-dispersed Bi<SUB>0.4</SUB>Sb<SUB>1.6</SUB>Te<SUB>3</SUB> are attributed to the grain boundary hardening and second-phase hardening. Beyond thermoelectric materials, our result implies that the grain refinement by nanoparticle dispersion is a simple and promising way to strengthen the mechanical properties of other brittle materials with layered structure.

Efficient xeno-free culture system for human embryonic stem cells

Baek J.A.,Seol H.W.,Jung J.,Yoon B.A.,Kim H.S.,Oh S.K.,Koo S.,Kim S.H.,Moon S.Y.,Choi Y.M. 한국발생생물학회 2011 한국발생생물학회 학술발표대회 Vol.30 No.-

The development of humanized culture system of human embryonic stem cells (hESCs) hold promise for therapeutic applications. However, conventional culture system contain animal-derived components such as fetal bovine serum and mouse embryonic fibroblasts that bear a risk of transmitting non-human pathogens and incorporation of non-human immunogenic molecules to hESCs. In this study, we developed an efficient xeno-free hESCs culture system using humanized materials, the CELLstartTM, human foreskin feeder and xeno-free medium containing knockOutTM SR XenoFree (XF-medium) without animal-derived material. The hESCs were gradually adapted to the XF-medium; 25:75, 50:50, 75:25 and 100:0. Two karyotypically normal hESC lines, SNUhES4 and H1, were used for the experiments of xeno-free culture condition. The attachment rates at xeno-free culture system were 52.6±12.4%, 67.0±16.6%, 59.0±13.9%, 28.3±2.9% in SNUhES4, 79.3±5.4%, 53.8±20.9%, 69.4 ±6.4%, 59.8±12.6% in H1 and the spontaneous differentiation rates were 42.2±12.7%, 31.4±2.9%, 40.8±14.5%, 55.2±35.5% in SNUhES4, 35.6±8.5%, 36.4±13.5%, 48.4±7.8%, 80.1±6.0% in H1 in the first four passage. Although the attachment rates were low and the spontaneous differentiation rates were high compared to that of conventional system in the early passages using this humanized culture condition, hESCs in this culture condition were found to maintain hESC characterizations; morphology, expression of cell surface markers and stable karyotype. Our results indicate that simplified compositions of humanized culture system can be applicable to the further optimization for a xeno-free culture of hESCs without the loss of pluripotency and contamination from xenogenic sources.

KDM3B Is the H3K9 Demethylase Involved in Transcriptional Activation of imo2 in Leukemia

Kim, J.-Y.,Kim, K.-B.,Eom, G.H.,Choe, N.,Kee, H.J.,Son, H.-J.,Oh, S.-T.,Kim, D.-W.,Pak, J.H.,Baek, H.J. AMERICAN SOCIETY FOR MICROBIOLOGY 2012 Molecular and cellular biology Vol.32 No.14

Estrus synchronization affects galectin-3, FGF-9 signaling in the sow reproductive tract

Baek S. Y,Kim D. W,Min Y. J,Cho E. S,Choi T. J,Soh H. C,Kim Y. M,Kang S. J,Kim B. K,Cho K. H,Cho K. H1 한국수정란이식학회 2017 한국수정란이식학회 학술대회 Vol.2017 No.05

The purpose of this study was to investigate the effect of estrus synchronization to altrenogest regumate (progesterone), PMSG/hCG, and artificial insemination (AI) on galectin-3, FGF-9 gene and protein expression. The morpho-metrical parameters of the endometrium and the number of corpora lutea (CL) were recorded. RNA was isolated from endometrial, oviduct and ovary tissues of non-synchronized (Control; n = 7) and AI synchronized (regumate, PMSG/hCG; n = 7) sows. The total number of CL was higher (P<0.05) in pigs treated with regumate/PMSG/hCG. The content of gelactin-3 and FGF-9 mRNA in pre-embryonic development stages increased on particular days, in control and studied in regumate/PMSG/hCG administered pigs. Gelactin-3 and FGF-9 were affected by regumate/PMSG/hCG treatment in the both pre-embryonic development stages (P<0.001, P<0.05) and encdometrial tissue (P<0.001, P<0.01). The regumate/PMSG/hCG treatment resulted in elevated expression of gelactin-3 (P<0.001) and FGF-9 (P<0.005) in oviduct and ovary tissues in comparison to control sows. Moreover, oviduct amount of gelectin-3 mRNA was higher in regumate/PMSG/hCG sows in comparison to the control group (P<0.05), whereas, expression characteristics of gelactin-3 and FGF-9 were investigated by hematoxylin and eosin stained and immunohistochemical staining. The results showed that galectin-3 and FGF-9 were significantly shown in the endometrium, oviduct and ovary tissues of the regumate/PMSG/ hCG. Presented data show that exogenous hormones administration can affect gene and protein expression in the sow reproductive tract.

B12-O-07 Self-relaxed conductive filament in ReRAM analyzed by in-situ TEM and Atom Probe Tomography

Chae, B. G.,Baek, K. J.,Song, J. H.,Hwang, H. S.,Oh, S. H.,Seol, J. B.,Park, C. G. Oxford University Press 2015 Microscopy Vol.64 No.suppl1

BLDC 모터의 자극위치 검출 보상기를 이용한 PV 양수 시스템

백승길,조정민,이승환,김성남,오봉환,이훈구,한경희 明知大學校 産業技術硏究所 2002 産業技術硏究所論文集 Vol.21 No.-

This paper shows how to design a Global control using Field Orientation, avoiding to use the DC/DC converter, and finding the relationships among the DC magnitudes and AC ones. And this paper deals with water pumping system which economic and durable storage systems for sustained production of power and propose MPPT methods using iqs of BLDC motor without battery module and chopper circuit.

Identification and quantification of glucosinolates in Korean leaf mustard germplasm (Brassica juncea var. integrifolia) by liquid chromatography–electrospray ionization/tandem mass spectrometry

Kim, H. W.,Ko, H. C.,Baek, H. J.,Cho, S. M.,Jang, H. H.,Lee, Y. M.,Kim, J. B. Springer Science + Business Media 2016 European food research and technology Vol.242 No.9

<P>In the present investigation, glucosinolate content was identified and quantified in 210 accessions of Korean leaf mustard (Brassica juncea var. integrifolia) by a liquid chromatography (LC) with electrospray ionization (ESI) coupled with a positive-mode ion trap tandem mass spectrometry (LC-ESI-MS/MS). Eleven individual GSLs including two new compounds were identified in the accessions using desulfo-glucosinolates (DS-GSLs) LC-MS library, and they were elucidated by interpreting the fragmentation data ([M + H-glucose](+)) obtained. The newly identified GSLs contained aliphatic and hydroxyl functional group in their back bone were named as 1-ethyl-2-hydroxyethyl DS-GSLs (glucosisautricin) or 2-hydroxy-2-methylpropyl DS-GSLs (glucoconringiin) and 3,4,5-trimethoxybenzyl DS-GSLs, respectively. Among all tested accessions, the total DS-GSLs content was found to be varied between 5.3 and 23.2 mg/g dry weight (DW) with an average of 13.0 mg/g DW in the germplasm. Each individual DS-GSLs component was found in decreasing order of sinigrin (41.7 %) > glucoiberverin (21.7 %) > gluconasturtiin (12.6 %) > glucobarbarin (10.0 %) > glucoiberin (5.1 %) > glucocheirolin (3.6 %) > glucobrassicanapin (2.6 %) > gluconapin (2.1 %), and > glucobrassicin (0.6 %). Interestingly, sinigrin and gluconasturtiin were present in higher content, and progoitrin was not detected significantly in the germplasm of Korean leaf mustard. In particular, accession K046197 (purple) was found to show highest total DS-GSL content (23.2 mg/g DW).</P>

Improved reversible redox cycles on MTiOx (M=Fe, Co, Ni, and Cu) particles afforded by rapid and stable oxygen carrier capacity for use in chemical looping combustion of methane

Kwak, B.S.,Park, N.K.,Ryu, S.O.,Baek, J.I.,Ryu, H.J.,Kang, M. Elsevier 2017 CHEMICAL ENGINEERING JOURNAL -LAUSANNE- Vol.309 No.-

In this study, rutile TiO<SUB>2</SUB> particles (MTiOx) incorporating 3d-metals, with their electrons more than half-filled, are synthesized for use in the chemical looping combustion of methane. The specific structures are obtained by doping the M-sites with Fe, Co, Ni, and Cu (FeTiOx, CoTiOx, NiTiOx, and CuTiOx), and they have the same M/Ti molar ratio of 1.0. XRD, SEM, CH<SUB>4</SUB>-TPD, TGA, and BET techniques were used to characterize the prepared materials. The XRD results confirmed that all of the prepared materials possessed pure hexagonal phases in the case of Co1Ti1Ox and Ni1Ti1Ox and mixed formations composed of metal oxides and rutile TiO<SUB>2</SUB> in the case of Fe1Ti1Ox and Cu1Ti1Ox. The CH<SUB>4</SUB>-TPD and H<SUB>2</SUB>-TPR analyses showed that the highest adsorption is obtained on CuTiOx, in particular on Cu1.5Ti1Ox. The oxygen carrier capacities of the samples were measured in H<SUB>2</SUB> (FR)/air (AR) and CH<SUB>4</SUB>-CO<SUB>2</SUB> (FR)/air (AR) redox systems at 850<SUP>o</SUP>C using TGA analysis. Their variability was shown to be associated with the different doping elements and doping concentrations. In particular, the Cu1.5Ti1Ox material in the CH<SUB>4</SUB>-CO<SUB>2</SUB> (FR)/air (AR) redox system exhibits oxygen uncoupling behaviour, leading to improved capture and release of oxygen compared to the other materials.