Dual aptamer-functionalized silica nanoparticles for the highly sensitive detection of breast cancer

Jo, H.,Her, J.,Ban, C. Elsevier Applied Science 2015 Biosensors & bioelectronics Vol.71 No.-

In this study, we synthesized dual aptamer-modified silica nanoparticles that simultaneously target two types of breast cancer cells: the mucin 1 (MUC1)(+) and human epidermal growth factor receptor 2 (HER2)(+) cell lines. Dual aptamer system enables a broad diagnosis for breast cancer in comparison with the single aptamer system. The dye-doped silica nanoparticles offer great stability with respect to photobleaching and enable the accurate quantification of breast cancer cells. The morphological and spectroscopic characteristics of the designed Dual-SiNPs were demonstrated via diverse methods such as DLS, zeta potential measurements, UV-vis spectroscopy, and fluorescence spectroscopy. Negatively charged Dual-SiNPs with a homogeneous size distribution showed robust and strong fluorescence. In addition, Dual-SiNPs did not affect cell viability, implying that this probe might be readily available for use in an in vivo system. Through ratio optimization of the MUC1 and HER2 aptamers, the binding capacities of the Dual-SiNPs to both cell lines were maximized. Based on Dual-SiNPs, a highly sensitive quantification of breast cancer cells was performed, resulting in a detection limit of 1cell/100μL, which is significantly lower compared with those reported in other studies. Moreover, the developed detection platform displayed high selectivity for only the MUC1(+) and HER2(+) cell lines. It is expected that this valuable diagnostic probe will be a noteworthy platform for the diagnosis and prognosis of breast cancer.

초등학교 1학년 통합단원 "함께하는 한가위" 재구성 실행연구

신혜리 ( Her Yee Shin ),홍영기 ( Young Ki Hong ) 한국통합교육과정학회 2010 통합교육과정연구 Vol.4 No.1

본 연구는 초등학교 1학년 2학기 ``바른 생활``, ``슬기로운 생활``, ``즐거운 생활``과의 통합 단원인 ``함께하는 한가위``를 통합적 관점에서 재구성하여 실행한 연구이다. 34명의 초등학교 1학년 학생을 대상으로 재구성한 통합단원을 교사연구자가 실행하는 과정에서 일어나는 교사의 교육과정 재구성에 대한 인식과 학생들의 수업태도 및 참여의 변화를 이해하고 묘사하는데 초점을 두었다. 통합단원의 재구성은 한 차시의 내용에 ``바른 생활``, ``슬기로운 생활``, ``즐거운 생활``과의 내용을 학생들의 생활과 밀접한 통합 활동으로 재구성하여 통합교과가 한 단원 당 2주간 배정된 시량인 총 22차시로 재구성하여 적용하였다. 이를 위해 교수·학습과정안, 학생용 워크북, 교사용 수업자료 등을 개발하였다. 자료 수집은 연구일지, 참여관찰과 비구조화된 면담, 실행과정에서의 실행일지, 학생들이 작성한 학습일지, 동영상 및 사진촬영 등을 통해 이루어졌다. 연구를 통해 학생들은 학습에 있어 함께하는 방법을 알고, 학교는 즐거운 배움터라는 인식을 하였으며, 교사의 변화로는 학습자 중심 교육에 대한 인식변화, 학생을 바라보는 관점의 변화, 교육과정 재구성 시도에 대한 두려움의 극복 등이 있었다. This is an action research on restructuring the integrated unit, ``Sharing together Hangawee`` that consisted the same theme across three integrated subjects. The purpose of this study was to describe and understand the changes of the action researcher`s awareness of curriculum restructuring and of the student participants` attitudes toward learning and classroom participation. Restructuring the integrated unit was mainly done by the way of designing activities closely related with students` real life and three integrated subjects of the first grade. The unit consisted of twenty-two class periods which was the standardized class period for one unit. For the implementation, teaching materials such as lesson plans, student workbook, powerpoints, hand-outs were developed and applied. Based on the research, the changes of the student were ``knowing how to cooperate with peers`` and ``recognizing school as a place of enjoyable learning``. The action researcher changes her paradigm on student-centered instruction and on the viewpoints of the student as a learner. In addition, her ability of curriculum restructuring was improved.

심리기술훈련이 경쟁 상태 불안과 운동 수행에 미치는 영향

주동엽(Joo Dong-Yeup),범대진(Bhum Dae-Jin),허재호(Her Jeo-Ho) 한국체육과학회 2000 한국체육과학회지 Vol.9 No.2

The purpose of this study was to investigate the effects of psychological skills training on competitive state anxiety and sport performance. The participants were 76 physical education students (40 males, 36 females) ranging in age from 18 to 27 from the Dong-shin University in Naju city. In order to measure competitive state anxiety, the Competitive State Anxiety Inventory-2 (CSAI-2) was used. It was composed of 27 questions covering cognitive, physical instabilities and self-confidence respectively with 9 questions. To measure sports performance, the participants were performed golf putting. Prior to performing the golf putting, the participants completed the CSAI-2 to measure competitive state anxiety and a test of golf putting. After pre-test, the participants divided randomly four different groups to received 16 weeks psychological skills training. One of the groups was a control group and the others were three experimental groups. Three experimental groups were performed a 16 weeks psychological skills training program by using the Progressive Muscle Relaxation and the Imagery Training. The control group was not contacted in any way during the 16 weeks. After 16 weeks, the participants were re-tested using the same methods as in the pre-test to examine the effects of the psychological skills training. The results were as follows: 1) The psychological skills training programs were effective in reducing anxiety levels of the participants. Three experimental groups were meaningful for reducing his/her cognitive anxiety. Its were also effective for lessening his/her physical instability and improving self-confidence. 2) The psychological skills training programs were effective in improving the golf putting scores. The score gap between the control group and three experimental groups were particularly large. This means that the psychological skills training programs were affected to his/her competitive state anxiety, thereby contributing to improving scores in the Golf putting' performance.

Viewing Angle Switching in In-Plane Switching Liquid Crystal Display

Her, Jung Hwa,Shin, Suck Jae,Lim, Young Jin,Bhattacharyya, Surjya Sarathi,Kang, Wan-Seok,Lee, Gi-Dong,Lee, Seung Hee Informa UK (TaylorFrancis) 2011 Molecular Crystals and Liquid Crystals Vol.544 No.1

L' engendrement du texte La Fabrique du Pre

Her, Jung A 한국불어불문학회 1982 佛語佛文學硏究 Vol.17 No.1

Superhydrophobic Transparent Surface of Nanostructured Poly(Methyl Methacrylate) Enhanced by a Hydrolysis Reaction

Her, E. K.,Ko, T.‐,J.,Shin, B.,Roh, H.,Dai, W.,Seong, W. K.,Kim, H.‐,Y.,Lee, K.‐,R.,Oh, K. H.,Moon, M.‐,W. WILEY‐VCH Verlag 2013 Plasma Processes and Polymers Vol.10 No.5

<P><B>Abstract</B></P><P>We report a method to create a self‐cleaning, transparent surface on poly(methyl methacrylate) (PMMA) using a dry etching with CF<SUB>4</SUB> plasma and a subsequent hydrolysis process with water immersion. During the CF<SUB>4</SUB> plasma treatment, nanoscale pillar structures were formed and its aspect ratio tended to increase. However, the transparency was reduced due to the deposition and the accumulation of undesired capping layers, which cover the structures. The hydrolysis was used to remove the capping layers of metal fluoride. As a result, the surface improves their clarity based on the transmittance up to 95%, because the reflectivity was reduced. With a subsequent hydrophobic coating, a superhydrophobic surface was achieved showing robust anti‐wetting and anti‐dewing effects.</P>

Integrated control of the differential braking, the suspension damping force and the active roll moment for improvement in the agility and the stability

Her, Hyundong,Suh, Jeeyoon,Yi, Kyongsu Professional Engineering Publishing Ltd 2015 Proceedings of the Institution of Mechanical Engin Vol. No.

<P>This paper describes an integrated control strategy of the differential braking, the semiactive suspension damper and the active roll moment. Integrated control is designed to improve the agility and the stability of a vehicle by simultaneous control of the yaw rate and the roll motion. The proposed integrated chassis control algorithm consists of a supervisor, vehicle motion control algorithms and a coordinator. The supervisor monitors the vehicle status and determines the desired vehicle motions such as the desired yaw rate and the desired roll motion based on control modes to improve the stability and the agility of the vehicle. The control algorithm calculates the desired yaw moment and the desired roll moment for generation of the desired vehicle motions. The coordinator determines the brake pressures, the damper current and the active roll moment to generate the desired yaw moment and the desired roll moment. The roll moment and the yaw moment can be controlled simultaneously by the use of the active anti-roll bar motor torque. Open-loop and closed-loop simulations of a driver–vehicle–controller system were conducted to investigate the performance of the proposed control algorithm. The performance of the proposed integrated chassis control algorithm was compared with those of the individual chassis control systems, such as the electronic control suspension and the an active roll control system. It was shown from the simulation results that the proposed integrated chassis control system can provide a significantly improved performance with respect to the agility, the manoeuvrability and the roll stability of a vehicle compared with the performances of the individual chassis control systems.</P>

Synthesis of Conductive Nano Ink Using 1-Octanethiol Coated Copper Nano Powders in 1-Octanol for Low Temperature Sintering Process

Her, J.,Cho, D.,Lee, C.S. JAPAN INSTITUTE OF METALS 2013 Materials transactions Vol.54 No.4

New Design Approaches for Ultrasonic Reactors: Degradation of Naphthalene and Phenol in Water

Her, Nam-Guk,Park, Jong-Sung,Oh, Jeill,Yoon, Yeomin Springer-Verlag 2011 Water, air, and soil pollution Vol.220 No.1

Bioinspired steel surfaces with extreme wettability contrast.

Her, Eun Kyu,Ko, Tae-Jun,Lee, Kwang-Ryeol,Oh, Kyu Hwan,Moon, Myoung-Woon RSC Pub 2012 Nanoscale Vol.4 No.9

<P>The exterior structures of natural organisms have continuously evolved by controlling wettability, such as the Namib Desert beetle, whose back has hydrophilic/hydrophobic contrast for water harvesting by mist condensation in dry desert environments, and some plant leaves that have hierarchical micro/nanostructures to collect or repel liquid water. In this work, we have provided a method for wettability contrast on alloy steels by both nano-flake or needle patterns and tuning of the surface energy. Steels were provided with hierarchical micro/nanostructures of Fe oxides by fluorination and by a subsequent catalytic reaction of fluorine ions on the steel surfaces in water. A hydrophobic material was deposited on the structured surfaces, rendering superhydrophobicity. Plasma oxidization induces the formation of superhydrophilic surfaces on selective regions surrounded by superhydrophobic surfaces. We show that wettability contrast surfaces align liquid water within patterned hydrophilic regions during the condensation process. Furthermore, this method could have a greater potential to align other liquids or living cells.</P>